Body Fat Deposition Research Articles

Validation of a mechanistic dynamic pre-weaned lamb growth and body composition simulation model

Lamb growth and body composition simulation models can provide valuable insights into the assessment of feeding regimens and rearing systems, allowing optimisation of farm profitability without the need for in-vivo trials. The objective of the present study was to evaluate the performance of a published mechanistic dynamic model on the growth and body composition of pre-weaned lambs using independently published data sets. Data generated from eight treatments, representing three artificial lamb rearing experiments (n = 77 lambs) were used. Initial body composition data were obtained from four lambs at approximately two days of age, with the remaining lambs being provided with defined nutritional treatments until the trial end-point. The body composition of lambs at slaughter was determined in six treatments across two experiments. Feed intake and live weight (LW) of lambs were recorded in all treatments. The LW, average daily gain (ADG) and ash deposition rates of lambs were accurately simulated by the model (paired t-test, P > 0.05). The overall empty body weight (EBW), gutfill, protein and fat deposition rates were overestimated, and water deposition rate was underestimated by the model (paired t-test, P < 0.05). Collectively, these results suggest that the model can be used at the farm level with acceptable accuracy to determine lamb growth. Further improvements in the model are required for protein, fat and water deposition rates, gutfill and EBW to make it more applicable for wider usage.

Effect of xylo-oligosaccharides on reproduction, lipid metabolism, and adipokines of hens during the late egg-laying period.

The present study aimed to investigate the effect of xylo-oligosaccharides (XOS) administration on egg production, reproductive hormones, serum lipids and adipokines of hens at the late cycle of reproduction. Four treatments included control (basal diet) and XOS addition at 2.0 (XOS-2), 4.0 (XOS-4), or 6.0 (XOS-6) g/kg of diet using 288 commercial Hy-Line brown hens from 73 to 84 wk of age. Egg production, body fat deposition, reproductive tract and hormones, lipid metabolism and adipokines were determined. At 84 wk, compared to the control, XOS supplementation at the three doses increased (p<0.001) egg-laying rates by 13.2% averagely, which led to a higher egg mass by 131 g/hen throughout the whole trial period. Abdominal fat and skinfold of XOS treatments were decreased (p<0.001) by 26.1% and 18.6%, respectively; large follicles and ovary weight were increased (p<0.001) by 0.73 follicle/hen and 18.6%, respectively. For serum parameters, cholesterol and triglyceride were decreased (p<0.001) by 17.5% and 29.2%, respectively; luteinizing hormone, follicle-stimulating hormone, and progesterone were increased (p≤0.001) by 16%, 31%, 29%, respectively; adiponectin and visfatin were increased (p<0.001) by 34% and 44%, respectively; but chemerin and leptin were decreased (p≤0.001) by 22% and 14%, respectively. With the increased XOS doses, linear decreases (p<0.05) were found on abdominal skinfold and serum triglyceride. The obtained data indicate that XOS can be used as an additive to improve fecundity by beneficially modulating fat deposition, lipid metabolism, reproductive hormones, and adipokines of hens at the late cycle of reproduction.

Correlation between body condition score and body composition in a rat model for obesity research

The incidences of obesity-associated chronic diseases are increasing worldwide. Research into the causes of obesity as well as potential treatments has highlighted the crucial role of preclinical studies using animal models. Rats are one of the most widely used species in obesity research. However, even with decades of research in both genetically obese rats and diet-induced obese rat models, definitive criteria to practically classify levels of obesity in the rat are not well established. The current study proposes new criteria modified from a 5-point body condition score (BCS) using in an animal health monitoring system and added a half-point scale to extend the range of body weight associated with subcutaneous fat deposition. The modified criteria were tested and compared with body composition from dual energy X-ray absorptiometry scans and selected adipose tissue weights. The results showed that the modified body condition scale was highly correlated with fat deposition in the rat body, particularly the visceral and inguinal fat pads. Both pads were closely related to changes in some specific landmarks used for the scale determination. These finding should extrapolate to obese rats in other models, with the advantage that data classified in BCS can pair the animal data with human body mass index. This will enhance the value of information from preclinical studies to design and predict outcomes of subsequent human clinical trials.

Daisaikoto improves fatty liver and obesity in melanocortin-4 receptor gene-deficient mice via the activation of brown adipose tissue

Melanocortin 4 receptor gene-knockout (MC4R-KO) mice are known to develop obesity with a high-fat diet. Meanwhile, daisaikoto, one of Kampo medicines, is a drug that is expected to have therapeutic effects on obesity. Here, we report the efficacy of daisaikoto in MC4R-KO mice. Eight-week-old MC4R-KO male mice (n = 12) were divided into three groups as follows: the SD group, which is fed with a standard diet; the HFD group, fed a high-fat diet; and the DSK group, fed with a high-fat diet containing 10% of daisaikoto. After the four-week observation period, mice in each group were sacrificed and samples were collected. The body weights at 12 weeks were significantly higher in the HFD group than in the other groups, indicating that daisaikoto significantly reduced body weight gain and fat deposition of the liver. The metabolome analysis indicated that degradation of triglycerides and fatty acid oxidation in the liver were enhanced by daisaikoto administration. In MC4R-KO mice, the cytoplasm and uncoupling protein 1 expression of brown adipose tissue was decreased; however, it was reversed in the DSK group. In conclusion, daisaikoto has potentially improved fatty liver and obesity, making it a useful therapeutic agent for obesity and fatty liver.

Dietary Succinate Impacts the Nutritional Metabolism, Protein Succinylation and Gut Microbiota of Zebrafish

Succinate is widely used in the food and feed industry as an acidulant, flavoring additive, and antimicrobial agent. This study investigated the effects of dietary succinate on growth, energy budget, nutritional metabolism, protein succinylation, and gut microbiota composition of zebrafish. Zebrafish were fed a control-check (0% succinate) or four succinate-supplemented diets (0.05, 0.10, 0.15, and 0.2%) for 4 weeks. The results showed that dietary succinate at the 0.15% additive amount (S0.15) can optimally promote weight gain and feed intake. Whole body protein, fat, and energy deposition increased in the S0.15 group. Fasting plasma glucose level decreased in fish fed the S0.15 diet, along with improved glucose tolerance. Lipid synthesis in the intestine, liver, and muscle increased with S0.15 feeding. Diet with 0.15% succinate inhibited intestinal gluconeogenesis but promoted hepatic gluconeogenesis. Glycogen synthesis increased in the liver and muscle of S0.15-fed fish. Glycolysis was increased in the muscle of S0.15-fed fish. In addition, 0.15% succinate-supplemented diet inhibited protein degradation in the intestine, liver, and muscle. Interestingly, different protein succinylation patterns in the intestine and liver were observed in fish fed the S0.15 diet. Intestinal proteins with increased succinylation levels were enriched in the tricarboxylic acid cycle while proteins with decreased succinylation levels were enriched in pathways related to fatty acid and amino acid degradation. Hepatic proteins with increased succinylation levels were enriched in oxidative phosphorylation while proteins with decreased succinylation levels were enriched in the processes of protein processing and transport in the endoplasmic reticulum. Finally, fish fed the S0.15 diet had a higher abundance of Proteobacteria but a lower abundance of Fusobacteria and Cetobacterium. In conclusion, dietary succinate could promote growth and feed intake, promote lipid anabolism, improve glucose homeostasis, and spare protein. The effects of succinate on nutritional metabolism are associated with alterations in the levels of metabolic intermediates, transcriptional regulation, and protein succinylation levels. However, hepatic fat accumulation and gut microbiota dysbiosis induced by dietary succinate suggest potential risks of succinate application as a feed additive for fish. This study would be beneficial in understanding the application of succinate as an aquatic feed additive.

Dietary Inclusion of Seabuckthorn (Hippophae rhamnoides) Mitigates Foodborne Enteritis in Zebrafish Through the Gut-Liver Immune Axis.

To help prevent foodborne enteritis in aquaculture, several feed additives, such as herbal medicine, have been added to fish diets. Predictions of effective herb medicines for treating fish foodborne enteritis from key regulated DEGs (differentially expressed genes) in transcriptomic data can aid in the development of feed additives using the Traditional Chinese Medicine Integrated Database. Seabuckthorn has been assessed as a promising candidate for treating grass carp soybean-induced enteritis (SBMIE). In the present study, the SBMIE zebrafish model was used to assess seabuckthorn’s therapeutic or preventative effects. The results showed that intestinal and hepatic inflammation was reduced when seabuckthorn was added, either pathologically (improved intestinal villi morphology, less oil-drops) or growth-related (body fat deposition). Moreover, seabuckthorn may block the intestinal p53 signaling pathway, while activating the PPAR signaling pathway and fatty acid metabolism in the liver. 16S rRNA gene sequencing results also indicated a significant increase in OTU numbers and skewed overlapping with the fish meal group following the addition of seabuckthorn. Additionally, there were signs of altered gut microbiota taxa composition, particularly for reduced TM7, Sphingomonas, and Shigella, following the addition of seabuckthorn. Hindgut imaging of fluorescent immune cells in SBMIE larvae revealed the immune regulatory mechanisms at the cellular level. Seabuckthorn may significantly inhibit the inflammatory gathering of neutrophils, macrophages, and mature T cells, as well as cellular protrusions’ formation. On the other hand, in larvae, seabuckthorn inhibited the inflammatory aggregation of lck+ T cells but not immature lymphocytes, indicating that it affected intestinal adaptive immunity. Although seabuckthorn did not affect the distribution of intestinal CD4+ cells, the number of hepatic CD4+ cells were reduced in fish from the seabuckthorn supplementation group. Thus, the current data indicate that seabuckthorn may alleviate foodborne gut-liver symptoms by enhancing intestinal mucosal immunity and microbiota while simultaneously inhibiting hepatic adipose disposition, making it a potential additive for preventing fish foodborne gut-liver symptoms.

Lactobacillus paracasei 24 Attenuates Lipid Accumulation in High-Fat Diet-Induced Obese Mice by Regulating the Gut Microbiota.

Obesity has become a worldwide public health problem. Lactic acid bacteria have attracted extensive attention for alleviating obesity and fat accumulation. This study aimed to evaluate the alleviating effects of Lactobacillus paracasei 24 (LP24) on lipid accumulation in an obese mouse model induced by a high-fat diet (HFD). The results showed that LP24 treatment significantly reduced body weight and fat deposition in HFD mice, improved blood lipid levels and liver steatosis, reduced liver oxidative stress injury and the inflammatory response, and regulated fat metabolism-related factors. Moreover, LP24 regulated the abundance and diversity of the gut microbiota, reduced the abundance of Firmicutes and the ratio of Firmicutes/Bacteroidetes (F/B), and increased the abundance of Akkermansia. In summary, LP24 regulates lipid metabolism by activating the expression level of related genes and regulating the gut microbiota through the gut-liver axis to attenuate the development of obesity. This study provides a theoretical basis for probiotics to regulate gut microbiota to reduce lipid accumulation.

Dietary Supplementation With Fine-Grinding Wheat Bran Improves Lipid Metabolism and Inflammatory Response via Modulating the Gut Microbiota Structure in Pregnant Sow.

This study investigated the effects of fine-grinding wheat bran on pregnant sow body condition, lipid metabolism, inflammatory response, and gut microbiota. In this study, wheat bran was crushed into three particle sizes. A total of 60 Landrace × Yorkshire second parity sows were allotted to two groups: CWB (a diet containing coarse wheat bran with particle size of 605 μm) and FWB (a diet containing fine wheat bran with particle size of 438 μm). Fine-grinding wheat bran had higher soluble dietary fiber concentration, swelling capacity, water-holding capacity, and fermentability than coarse wheat bran. Pregnant sows fed FWB throughout pregnancy had lower body weight and fat deposition than sows fed CWB. And the piglet body weight at birth of the FWB group was remarkably increased. Serum concentrations of lipids (triglycerides, total cholesterol, and free fatty acid), interleukin 6, leptin, and resistin were decreased on day 90 of pregnancy by fine wheat bran supplementation. Feeding FWB significantly decreased abundance of Firmicutes and dramatically increased the abundance of Bacteroidetes at phylum level. At genus level, the abundance of Terrisporobacter was decreased in FWB feeding sows, but the abundance of Parabacteroides was increased. Fecal total short-chain fatty acids, propionate, and butyrate contents were markedly increased in the FWB group. The results suggested that the physicochemical properties of finely ground wheat bran had been improved. Dietary supplementation with fine wheat bran changed the gut microbiota structure and enhanced the short-chain fatty acids level, which improved the maternal body condition, metabolic and inflammatory status, and reproductive performance in sows.

Association of early menarche with elevated BMI, lower body height and relative leg length among 14- to 16-year-old post-menarcheal girls from a Maya community in Yucatan, Mexico

Human body segments have different timing and tempo of growth. Early menarche (EM) as an indicator of early reproductive maturity results in a shortened height and leg length. Relatively larger trunk may increase risk for more body fat deposit and higher body mass index (BMI) due to the allometry of total body fat with body proportions. The objective of the study was to assess the association of EM with BMI, absolute body size [height, sitting height (SH), subischial leg length (SLL)] and relative body dimensions [sitting height to subischial leg length ratio (SHSLLR), relative subischial leg length (RSLL)] among 14- to 16-year-old post-menarcheal girls from a rural Maya community in Quintana Roo, Yucatan, Mexico. In a cross-sectional study, post-menarcheal girls (n=51) aged 14 to 16 years had EM (n=22) (&lt;12 years of age) and not early menarche (NEM, n=29). Anthropometric measurements of height, weight, and SH were recorded. Derived variables were BMI, height and BMI-for-age z-scores, SLL, SHSLLR, and RSLL. Mean value of age at menarche (AM) was 13 years (EM 11 years, NEM 14 years). Mean values of height (EM 159 cm, NEM 164 cm), BMI (EM 20 kg/m2, NEM 19 kg/m2), sitting height (EM 81 cm, NEM 78 cm), SLL (EM 79 cm, NEM 85 cm), SHSLLR (EM 102.93%, NEM 92.03%), and RSLL (EM 49%, NEM 52%) were different (p&lt;0.05) in the two groups. BMI showed significant negative correlation with AM (Pearson’s r=-0.29, p&lt;0.04). Linear regression models adjusted for age showed that EM had different interrelationships (p&lt;0.05) with body dimensions: positive with BMI, SH, SHSLLR, and negative with height, SLL, and RSLL. Earlier AM was associated with higher BMI, SH, SHSLLR and lower SLL, RSLL, explaining lower body height and leg length among the participant EM girls. In the light of life history theory, EM results in a growth trade-off, short stature and larger trunk relative to leg length that might enhance risk for body fat gain.

Combination Effects of Metformin and a Mixture of Lemon Balm and Dandelion on High-Fat Diet-Induced Metabolic Alterations in Mice.

Metformin, the first-line drug for type 2 diabetes mellitus (T2DM), has additional effects on improvements of nonalcoholic fatty liver disease (NAFLD); however, there are no treatments for both T2DM and NAFLD. Previous studies have shown hepatoprotective effects of a mixture of lemon balm and dandelion (LD) through its antioxidant and anti-steatosis properties. Thus, combination effects of metformin and LD were examined in a high-fat diet (HFD)-induced metabolic disease mouse model. The model received an oral administration of distilled water, monotherapies of metformin and LD, or a metformin combination with LD for 12 weeks. The HFD-induced weight gain and body fat deposition were reduced more by the combination than either monotherapy. Blood parameters for NAFLD (i.e., alanine aminotransferase and triglyceride), T2DM (i.e., glucose and insulin), and renal functions (i.e., blood urea nitrogen and creatinine) were reduced in the combination. The combination further enhanced hepatic antioxidant activities, and improved insulin resistance via the AMP-activated protein kinase and lipid metabolism pathways. Histopathological analyses revealed that the metformin combination ameliorated the hepatic hypertrophy/steatosis, pancreatic endocrine/exocrine alteration, fat tissue hypertrophy, and renal steatosis, more than either monotherapy. These results suggest that metformin combined with LD can be promising for preventing and treating metabolic diseases involving insulin resistance.

Ramadan Diurnal Intermittent Fasting Is Associated With Attenuated FTO Gene Expression in Subjects With Overweight and Obesity: A Prospective Cohort Study.

Aim and BackgroundA growing body of evidence supports the impact of intermittent fasting (IF) on normalizing body weight and that the interaction between body genes and environmental factors shapes human susceptibility to developing obesity. FTO gene is one of these genes with metabolic effects related to energy metabolism and body fat deposition. This research examined the changes in FTO gene expression upon Ramadan intermittent fasting (RIF) in a group of metabolically healthy subjects with overweight and obesity.MethodsSixty-three (63) subjects were recruited, of which 57 (17 males and 40 females, mean age 38.4 ± 11.2 years) subjects with overweight and obesity (BMI = 29.89 ± 5.02 kg/m2were recruited and monitored before and at the end of Ramadan month), and 6 healthy subjects with normal BMI (21.4 ± 2.20 kg/m2) recruited only to standardize the reference for normal levels of FTO gene expression. In the two-time points, anthropometric, biochemical, and dietary assessments were undertaken, and FTO gene expression tests were performed using RNA extracted from the whole blood sample.ResultsIn contrast to normal BMI subjects, the relative gene expressions in overweight/obese were significantly decreased at the end of Ramadan (−32.30%, 95% CI–0.052 −0.981) in comparison with the pre-fasting state. Significant reductions were found in body weight, BMI, fat mass, body fat percent, hip circumference, LDL, IL-6, TNF-α (P<0.001), and in waist circumference (P<0.05), whilst HDL and IL-10 significantly increased (P<0.001) at the end of Ramadan in comparison with the pre-fasting levels. Binary logistic regression analysis for genetic expressions showed no significant association between high-energy intake, waist circumference, or obesity and FTO gene expression.ConclusionsRIF is associated with the downregulation of the FTO gene expression in subjects with obesity, and this may explain, at least in part, its favorable metabolic effects. Hence, RIF presumably may entail a protective impact against body weight gain and its adverse metabolic-related derangements in subjects with obesity.

Central body fat deposits are associated with poor vitamin D status in Chinese children and adolescents

ObjectivesTo examine the association between regional fat deposits and vitamin D levels in Chinese children. Whether regional fat distribution contributes to vitamin D deficiency remains uncertain. MethodsA population-based, nationwide and multicenter cross-sectional study involving 6,589 pediatric participants (49.9% boys) ages 6 to 18 y. The regional fat mass index (FMI) variables were calculated as regional fat mass (kg) divided by the square of height (m2), and plasma 25-hydroxyvitamin D (25[OH]D) levels were measured. ResultsAmong boys, after adjustment for multiple variables (including opposite regional fat), higher values for trunk FMI and android FMI were associated with a greater likelihood of vitamin D inadequacy (odds ratios [ORs], 2.14 and 1.87, respectively), whereas the extremity FMI and gynoid FMI were associated with protection from vitamin D inadequacy (ORs, 0.63 and 0.71, respectively). Consistently, increased central fat distribution (e.g., trunk or android to total fat ratio) and decreased peripheral fat distribution (e.g., extremity or gynoid to total fat ratio) were associated with greater odds of vitamin D inadequacy. Among girls, the associations of regional fat with vitamin D were weaker than those in boys, and most analyses did not reach statistical significance. ConclusionsRegional fat deposits were associated with vitamin D status in Chinese school-aged children and more pronounced among boys.

Selective targeting of chronic social stress-induced activated neurons identifies neurogenesis-related genes to be associated with resilience in female mice

Prolonged social stress is a major cause for depression in humans and is associated with a wide range of subsequent pathophysiological changes such as elevated blood pressure. A routinely used model for investigating this kind of stress in mice is the chronic social defeat paradigm where a smaller intruder is exposed to an aggressive inhabitant of a home cage. This model is restricted to males and includes a high proportion of physical stress that might e.g., interfere with immunological aspects of the stress. The prevalence of depression in humans is even higher in women than in men. Therefore, expanding models to female individuals is desirable. We here tested the social instability model as a tool for administering chronic social stress to female C57BL/6J mice and analyzed short-term as well as long-lasting effects. Animals were housed in groups of four and were shuffled two times a week, resulting in a permanent re-structuration of their social hierarchy. While directly after the stress exposure, serum corticosterone was elevated, increased body weight and fat deposits were observed in stressed mice even one year after discontinuation of the stress. At the behavioral level, animals could be stratified into resilient and susceptible animals directly post-stress, but those subgroups were not distinguishable any more in the long-term analysis. To identify molecular contributors to resilience in the here presented social instability induced stress model, Arc-activity dependent trapping of neurons was conducted in Arc-creERT2/sun1sfGFP mice. RNA samples derived from activated nuclei from the ventral hippocampus, a brain region involved in stress-regulation during attacks or explorative behavior of mice, were subjected to a neurogenesis pathway array. While several genes were differentially regulated by stress, in particular, artemin, a neurotrophic factor was upregulated in resilient versus susceptible individuals.

Neck circumference as a screening measure for identifying NAFLD among a group of academic employees in Bangkok, Thailand.

Neck circumference (NC) reflects the fat deposition in upper body and has potential to be used as a predictor of Non-Alcoholic Fatty Liver Disease (NAFLD). Our objectives were to examine the association of NC with NAFLD prevalence, and to determine the optimal cut-off of NC in identifying the presence of NAFLD among the employees of an academic institution in Bangkok, Thailand. In this cross-sectional study, 635 employees of an academic institution underwent anthropometric measurement and transient elastography following an overnight fast. NAFLD was defined as a CAP value >238 dB.m-1. The NAFLD prevalence in men and women were 66.17% and 46.22%, respectively. The mean NCs for men and women with NAFLD were higher (38.53±0.31 cm and 35.83±0.48 cm, respectively) than those without NAFLD (33.58±0.24 and 31.098±0.14 cm, respectively) (p<0.001). Metabolic markers including age, weight, BMI, NC, WC, WHR, FBS, triglycerides were significantly higher, HDL was significantly lower among participants with NAFLD compared to those without NAFLD (p<0.05). NC was independently associated with NAFLD among women with OR (95%CI) of 1.17 (1.05, 1.32). The optimal cut-offs of NC to predict NAFLD were 37.07 cm (sensitivity: 70.50%; specificity: 68.90%) and 32.07 cm (sensitivity: 70.70%; specificity: 62.10%), respectively for men and women. NC significantly correlated with NAFLD in women. The optimal cut-off points of 32 cm and 37 cm for men and women, which similar to Chinese populations. Therefore, it can be used as a cost-effective tool to predict NAFLD.Trial Registration: Thai Clinical Trials Registry (TCTR20210329006)

Exigência de valina dietética para tambaqui (Colossoma macropomum) com diferente peso corporal

ABSTRACT Tambaqui (Colossoma macropomum ) is one of the highly produced freshwater fish in Brazilian fish farming. However, its production efficiency associated with the refinement of diet formulations is limited due to a lack of information on nutritional requirements for certain essential amino acids such as valine. This study was designed to estimate the valine requirement of tambaqui having different body weights. A total of 720 juveniles were used in the feed trials. The first trial consisted of 360 fish with an average initial weight of 33.28 ±0.19 g, and the second trial comprised 360 fish (121.19 ±1.29 g). The trials consisted of dose-response experiments with five levels of valine (5.0, 7.0, 9.0, 11.0, and 13.0 g/kg diet) and four replicate tanks per treatment. The control diet was obtained by adding L-valine to the diet containing 5.0 g/kg diet (the first level of valine tested) to ensure it reached the diet containing 7.0 g/kg diet, corresponding to the second level tested. Analysis of variance, Dunnett’s test for comparison with the control diet, and regression analysis were performed. The results showed that the valine intake, weight gain, specific growth rate, and feed conversion ratio were significantly improved by the dietary valine (P &lt; 0.05) in both trials. Dietary levels of valine exerted quadratic effects on body protein, body fat, body protein deposition, and nitrogen retention efficiency of tambaqui with 33 g and 121 g as body fat deposition was affected by the dietary valine level only in tambaqui weighing 33 g. Quadratic regression analysis of body protein deposition data revealed the optimum dietary valine requirement of tambaqui (33.0 g-83.0 g) and (121.0 g-277.0 g) as 11.9 g/kg diet and 9.1 g/kg diet, respectively.

Межгрупповая изменчивость обхватных размеров тела и подкожного жироотложения у молодых мужчин различных этнических групп

Introduction. This study is devoted to intergroup variability of body circumferences, degree and to-pography of subcutaneous fat deposition in samples of young men of different ethnic backgrounds. Materials and Methods. We used anthropometric survey data from Russian men from various regions of Russia, indigenous Altaians and Tuvans from the Altai and Tyva Republics measured in the period from 2015 to 2019. Anthropometric measurements were performed according to standard methods of the Re-search Institute and the Museum of Anthropology of Moscow State University using GPM (DKSH, Switzer-land) and Holtain (Holtain Ltd, UK) anthropometric instruments. Body composition was assessed by bioelec-trical impedance analysis using ABC-02 Medass (Medass, Russia). We analyzed body circumferences, the value of fat and skin folds in various points of the body, bioimpedance measurements, and central fat dep-osition indices. Mathematical processing of the data was performed using the standard Statistica 10 soft-ware package. Discriminant (canonical) analysis was performed to study the topography of subcutaneous fat deposition. The materials have been collected from the population of these territories randomly and anonymously, according to the rules of bioethics and protocols of informed consent. Results. A comparative analysis of the main statistical parameters in three ethnic samples of men showed no significant differences in body mass index, the amount of fat mass, and most circumferences measurements. Intergroup variability was clearly evident in the thickness of fat and skin folds, in the index of central obesity (waist-to-height ratio), in the waist-to-hip ratio, and in the body adiposity index (BAI). In Tuvans, the value of fat and skin folds in the abdominal area and on the inner surface of the shoulder is significantly greater than in Russians and Altaians; in Russians, the mean values of all fat deposition indices are significantly lower than in Altaians and Tuvans. The canonical analysis of the set of body circumferences and fat and skin folds showed the predominant development of waist and shoulder circumferences and subcutaneous fat deposition on the body in Altaians and Tuvans as compared to Russians. Conclusions. The nature of intergroup variability indicates a predisposition of the Altaians and Tu-vans to the central type of fat deposition, which can be regarded as a result of adaptation under environ-mental stress in most Mongoloid populations of Southern Siberia.

Consumption of dietary fat causes loss of olfactory sensory neurons and associated circuitry that is not mitigated by voluntary exercise in mice.

Excess nutrition causes loss of olfactory sensory neurons (OSNs) and reduces odour discrimination and odour perception in mice. To separate diet-induced obesity from the consumption of dietary fat, we designed pair-feeding experiments whereby mice were maintained on isocaloric diets for 5months, which prevented increased fat storage. To test our hypothesis that adiposity was not a prerequisite for loss of OSNs and bulbar projections, we used male and female mice with an odorant receptor-linked genetic reporter (M72tauLacZ; Olfr160) to visualize neural circuitry changes resulting from elevated fat in the diet. Simultaneously we monitored glucose clearance (diagnostic for prediabetes), body fat deposition, ingestive behaviours, select inflammatory markers and energy metabolism. Axonal projections to defined olfactory glomeruli were visualized in whole-mount brains, and the number of OSNs was manually counted across whole olfactory epithelia. After being pair fed a moderately high-fat (MHF) diet, mice of both sexes had body weight, adipose deposits, energy expenditure, respiratory exchange ratios and locomotor activity that were unchanged from control-fed mice. Despite this, they were still found to lose OSNs and associated bulbar projections. Even with unchanged adipocyte storage, pair-fed animals had an elevation in TNF cytokines and an intermediate ability for glucose clearance. Albeit improving health metrics, access to voluntary running while consuming an ad libitum fatty diet still precipitated a loss of OSNs and associated axonal projections for male mice. Our results support that long-term macronutrient imbalance can drive anatomical loss in the olfactory system regardless of total energy expenditure. KEY POINTS: Obesity can disrupt the structure and function of organ systems, including the olfactory system that is important for food selection and satiety. We designed dietary treatments in mice such that mice received fat, but the total calories provided were the same as in control diets so that they would not gain weight or increase adipose tissue. Mice that were not obese but consumed isocaloric fatty diets still lost olfactory neuronal circuits, had fewer numbers of olfactory neurons, had an elevation in inflammatory signals and had an intermediate ability to clear glucose (prediabetes). Mice were allowed access to running wheels while consuming fatty diets, yet still lost olfactory structures. We conclude that a long-term imbalance in nutrition that favours fat in the diet disrupts the olfactory system of mice in the absence of obesity.

Growth and Body Composition of Artificially-Reared Lambs Exposed to Three Different Rearing Regimens.

Simple SummaryOptimum lamb growth and cost-effective artificial lamb rearing are important to maximise farm profit. In addition to milk feeding, supplementation of a solid feed with balanced nutrients and an adequate level of fibre will aid rumen development, allowing for a smooth transition from a liquid to a solely solid diet. This study aimed to determine the effect of pellet fibre level, milk replacer composition and early milk weaning at 42 days of age, on the growth and body composition of lambs reared artificially to 57 days of age. Results demonstrate that lamb growth rate to 57 days of age was not affected by early weaning or pellet fibre level. Early milk weaning increased pellet intake and empty rumen weight. Early weaning resulted in leaner carcasses due to reduced fat deposition and lower total feed costs compared to lambs offered milk to 57 days of age. Overall, feeding lambs either low or high fibre pellets and weaning early (42 days of age) was shown to be beneficial, as growth was similar to unweaned lambs, but leaner carcasses and reduced feed costs were observed.This study was designed to investigate the influence of pellet fibre level, milk replacer composition and age at weaning on growth and body composition of lambs reared artificially. Romney ram lambs were randomly allocated to one of three rearing treatments; HFP57: commercial milk replacer to 57 days of age, and high fibre concentrate pellets; HFP42: commercial milk replacer with early weaning at 42 days of age, and high fibre concentrate pellets; LFP42: high protein milk replacer from 2–16 days of age followed by commercial milk replacer with early weaning at 42 days of age, and low fibre concentrate pellets. Lambs were slaughtered at 57 days of age. Overall average daily liveweight gain of lambs did not differ (p > 0.05) between treatments. Dressing out percentage, carcass weight, empty small intestine and omental fat were higher (p < 0.05) in HFP57 than in both HFP42 and LFP42 lambs. HFP42 and LFP42 lambs had heavier (p < 0.05) empty rumen weights. Whole body protein content was higher (p < 0.05) in HFP42 lambs compared to both HFP57 and LFP42 lambs. Fat content and daily fat deposition were greater (p < 0.05) in HFP57 lambs than HFP42 and LFP42 lambs. Weaning lambs at 42 days of age with provision of either low or high fibre concentrate pellets, resulted in similar growth rates, reduced whole body fat deposition and was a more cost-effective rearing regimen.

The Influence of Obesity, Ovariectomy, and Greenshell Mussel Supplementation on Bone Mineral Density in Rats.

ABSTRACTObesity is considered to impair long‐term health by disturbing multiple physiological functions. However, it remains a controversial issue as to whether obesity has beneficial or detrimental effects on bone health in postmenopausal women. The aims of this study were to investigate the relationships between obesity and bone mineral density (BMD) under conditions of ovarian hormone deficiency in an animal model and to evaluate the potential health benefits of Greenshell mussel (GSM) on bone health. A total of 144 adult female Sprague–Dawley rats were fed from age 12 weeks on one of four diets (normal [ND]; ND + GSM; high fat/high sugar [HF/HS]; HF/HS + GSM; n = 36 per diet). At age 20 weeks, after a dual‐energy X‐ray absorptiometry (DXA) scan, 12 of the rats on each diet underwent ovariectomy (OVX) and the remaining rats were left intact. Twelve of the intact rats in each diet group were culled at age 26 weeks (short‐term cohort). The remaining rats were culled at age 48 weeks (long‐term cohort). Rats were DXA scanned before cull, then various fat pads were dissected. The results revealed that HF/HS rats and OVX rats dramatically increased body weight and fat deposition in correlation with leptin. In the long‐term cohort, vertebral spine BMD rapidly declined after OVX. At termination, the OVX rats had decreased plasma bone turnover markers of CTX‐1 and TRAP when compared with sham rats. Significantly higher BMD was found in OVX rats fed the HF/HS diet compared with ND, but this difference was not recapitulated in intact rats. BMD of right femur was significantly increased 5% to 10% by GSM in the short‐term cohort. The data demonstrated that obesity can be beneficial by increasing BMD in OVX rats, and this may extrapolate to postmenopausal women as adipocyte‐produced estrogen may slightly compensate for the reduction in ovarian hormones. Finally, the data showed that GSM may be beneficial to bone health by increasing BMD accrual. © 2021 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

Metabolizable energy levels in diets with a fixed nutrient: calorie ratio for free-range broilers

The present study proposes to examine the effect of dietary levels of metabolizable energy, under a fixed nutrient:calorie ratio, on the production performance; body fat and protein deposition; and carcass characteristics of free-range broilers from 1 to 84 days of age. Nine hundred unsexed chicks were allocated to six treatments in a completely randomized design with six replicates of 25 birds each. Treatments consisted of diets with varying levels of metabolizable energy (2700, 2800, 2900, 3000, 3100 and 3200 Kcal ME/kg of diet) and a fixed proportion of nutrients relative to the energy level according to the nutritional requirements for each rearing phase. Body weight, weight gain, feed intake, feed conversion, production viability, metabolizable energy intake, protein intake, lysine intake, body fat deposition, body protein deposition and carcass characteristics were evaluated. Data were subjected to analysis of variance and, later, to regression analysis. Increasing levels of metabolizable energy, coupled with a fixed nutrient:calorie ratio, reduced feed intake, increased body weight and weight gain, improved feed conversion and did not affect carcass characteristics. In conclusion, adjusting the nutrient supply according to the dietary energy level improves production performance by improving feed conversion, ensuring adequate nutrient intake and preserving fat and protein deposition in the carcass when the metabolizable energy level is raised up to 3200 Kcal/kg in all rearing stages.