Department Of Pharmaceutical Chemistry Research Articles

Corrigendum to: Review on Pyrazole Hybrids as Anti-microbial Agents

Similar affiliation error appeared in the author's affiliation in the article entitled “Review on Pyrazole Hybrids as Anti-microbial Agents”, published in Letters in Organic Chemistry, 2024, 21(4), 320-332 [1]. <p> Details of the error and a correction are provided here. <p> Original: <P> Author Affiliation: <sup>1</sup>Department of Pharmaceutical Chemistry, Bharati Vidyapeeth (Deemed to be) University, Poona College of Pharmacy, Pune, India; <sup>2</sup>Department of Pharmaceutical Chemistry, Bharati Vidyapeeth (Deemed to be) University, Poona College of Pharmacy, Pune, India <p> Corrected: <p> Author Affiliation: <sup>1</sup>Department of Pharmaceutical Chemistry, Bharati Vidyapeeth (Deemed to be) University, Poona College of Pharmacy, Pune, India <p> We regret the error and apologize to readers. <p> The original article can be found online at https://www.eurekaselect.com/article/133287

Characteristics of HIF-1Α and HSP70 MRNA Expression, Level, and Interleukins in Experimental Chronic Generalized Periodontitis.

Periodontal diseases are a rather complex problem of modern dentistry and do not have only medical but also social significance. The objective of this study is to weigh the effect of a mixture of Thiotriazoline and L-arginine (1:4) on the parameters of the system of endogenous cytoprotection of blood and periodontal illness in rats with experimental chronic generalized periodontitis and substantiate further study of this blend. The study aimed to evaluate the impact of a combination of Thiotriazoline and L-arginine (in a ratio of 1:4) on the parameters of the endogenous blood cytoprotection system and periodontium in rats with experimental chronic generalized periodontitis. A group of outbred rats weighing 190-220 g and sourced from the vivarium of the Institute of Pharmacology and Toxicology of the Academy of Medical Sciences of Ukraine were divided into four groups, each consisting of 10 animals. (1) Intact group, animals that were injected intragastrically with a solution of sodium chloride to chloride 0.9% for 30 days. (2) control, animals with experimental CGP who intragastrically sodium chloride solution 0.9% for 30 days. (3) animals with experimental CGP were injected intramuscularly with Thiotriazoline + L-arginine (1:4) in a dosage of 200 mg/kg (30 days). (4) animals with experimental CGP, for which daily intragastric reference drug Mexidol, in dosage 250 mg/kg (30 days). In this study, we utilized two substances: Thiotriazoline and L-arginine hydrochloride. The combination of Thiotriazoline and L-arginine (in a ratio of 1:4) was prepared at the Department of Pharmaceutical Chemistry of ZSMU. At the conclusion of the experiment, the rats were carefully removed from the study while under thiopental-sodium anesthesia, and administered at a dosage of 40 mg/kg. We have found that the administration of a combined preparation of Thiotriazoline with L-arginine to rats with CGP leads to a significant decrease in the blood concentration of pro-inflammatory cytokines IL-1b and TNF-a by 56.1% and 71%, respectively. The administration of Mexidol at a dosage of 250 mg/kg, as well as the combination of Thiotriazoline and Larginine in a ratio of 1:4 at a dosage of 200 mg/kg, resulted in a significant reduction in gingival pocket depth in animals with CGP. Specifically, the gingival pocket depth was reduced to 6 mm (p < 0.05) with Mexidol and further reduced to 4 mm (p < 0.05) with the combination of Thiotriazoline and L-arginine. Additionally, the animals exhibited minimal bleeding, swelling, and tooth mobility when treated with the combination of Thiotriazoline and L-arginine. The administration of a combination of Thiotriazoline and L-arginine (in a ratio of 1:4) at a dosage of 200 mg/kg to animals with CGP resulted in a noteworthy reduction in the blood concentration of pro-inflammatory cytokines IL-1b and TNF-a. Specifically, there was a significant decrease of 56.1% (p < 0.05) in IL-1b and 71% (p < 0.05) in TNF-a levels. The course administration of a combination of Thiotriazoline and L-arginine (1:4) (200 mg/kg) to animals with CGP led to an increased expression of HSP70 mRNA (p < 0.05) in the periodontium by 8.2 times and HIF-1a mRNA by 8.2 times. 2.8 times (p < 0.05) against the background of an increase in the blood concentration of HSP70 by 95% (p < 0.05). Also, in the periodontium of animals in this group, a decrease in the expression of c-Fos mRNA by 36.7% (p < 0.05) was found compared to the control group.

Total Polyphenolic Content, Antioxidant Activity and Chromatographic Profiling of Extracts of Ardissia solanacea

Pharmacognosy Research,2023,15,2,356-362.DOI:10.5530/pres.15.2.038Published:February 2023Type:Original Article Authors:Jyotsna Chopade, Asha Thomas, and Sohan Chitlange Author(s) affiliations:Jyotsna Chopade, Asha Thomas*, Sohan Chitlange Department of Pharmaceutical Chemistry, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pimpri, Pune, Maharashtra, INDIA. Abstract:Background: The current study focuses on the preliminary phytochemical analysis, antioxidant potential and identification of phytochemicals in Ardissia solanacea leaves extract using chromatography and HR-MS. Materials and Methods: The occurrence of phytoconstituents in different extracts of Ardissia solanacea were assessed by standard procedures. In vitro antioxidant potential was determined by Hydrogen peroxide and DPPH method. The extract was fractionated by Flash Chromatography. The fractions were analysed using HPTLC and further analyzed by HR-MS method to detect and characterize the active phytoconstituents present in it. Results: The highest concentration of flavonoids (371.91± 0.167 mg/g of Quercetin Equivalent), phenolics (10.138±0.010 mg/g of Gallic acid Equivalent) and tannins (148.23±0.510 mg/g of Tannic acid Equivalent) was observed in the ethanol extract. The antioxidant activity by DPPH and H2O2 assay demonstrated that the ethanolic extract (AsEt) showed highest anti-oxidant potential (74.21% and 83.16% respectively). The ASEt was fractionated by using Flash chromatography. As a result, Out of 11 fractions, one fraction namely AsEt3 was selected further based on HPTLC results. The HR-MS analysis of ASEt3 indicated presence of thirteen compounds wherein bergapten was predominantly identified. Conclusion: It was revealed that the leaves of Ardissia solanacea possesses high antioxidant potential. The known phytoconstituents namely quercetin and taxifolin along with one unknown compound were identified through the HR-MS study. Using mass library data, we report herein 2 flavonoids and 1 phenolic in the AsEt extract. Keywords:Antioxidant activity, Ardissia solanacea, Flash chromatography, HR-MS analysis.View:PDF (252.11 KB)

Development and Validation of Tranexamic Acid in Human Plasma by Using Ultra-Performance Liquid Chromatography-Mass Spectrometry

Harshini Shanmugam and Vijey Aanandhi Muthukumar Author informationArticle notesCopyright and License information Department of Pharmaceutical Chemistry and Analysis, Vels Institute of Science Technology and Advanced Studies (VISTAS), Chennai, Tamil Nadu, INDIA Corresponding author. *Correspondence: Vijey Aanandhi Muthukumar Department of Pharmaceutical Chemistry and Analysis, Vels Institute of Science Technology, and Advanced Studies (VISTAS), Chennai–600,117, Tamil Nadu,

Simultaneous Analysis of Lamivudine and Dolutegravir Sodium in Formulation Using First Order Derivative Method

Sapna M. Rathod, Paresh U. Patel and Nisarg C. Patel Author informationArticle notesCopyright and License information Department of Pharmaceutical Chemistry and Quality Assurance, APMC College of Pharmaceutical Education and Research, Himatnagar, Gujarat, INDIA Department of Pharmaceutical Chemistry and Quality Assurance, S. K. Patel College of Pharmaceutical Education and Research, Ganpat University, Kherva, Gujarat, INDIA Department

Assessment of Peripheral Neuropathy Pain by Biothesiometer in Diabetes Mellitus Patients

Manju Devi, Sukhdeep Singh, Meenakshi Dhanawat, Kirti Gupta, Sumeet Gupta, B K Agarwal, Inderjeet Verma and Parminder Nain Author informationArticle notesCopyright and License information Department of Pharmacy Practice, MM College of Pharmacy, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana, INDIA Department of Pharmaceutical Chemistry, MM College of Pharmacy, Maharishi Markandeshwar (Deemed to be

The life path of A.M. Khaletsky

Many brilliant scientists, professors, whose life and fate were directly connected with the history of the university, worked at the St. Petersburg State Chemical and Pharmaceutical University. Our national pharmacy owes their talent and enthusiasm.&#x0D; Based on unpublished archival records, the article reconstructs the biography, professional, scientific and social activities of Abram Mikhailovich Khaletsky, a wellknown chemist, professor, Doctor of Chemistry, who made a great contribution to the development of Russian science, who for many years worked as the Head of the Department of Pharmaceutical Chemistry of the Leningrad Pharmaceutical Institution. A graduate of the Medical Department of Kazan University, a student of Academy A.E. Favorsky, he took an active part in the reformation of the educational process of the LCPI. It was A.M. Khaletsky who proposed a new form of appraisal of pharmacists at that time the defense of theses, the first of them were performed at the Department of Organic Chemistry. Among the scientific achievements made under the leadership of A.M. Khaletsky: studies of isomeric changes of keto-alcohol, technology for the production of standard galenical preparations, the method of isolation of -sitosterol, improvement of hormone technology and the search for new hormonal drugs, and many others. Actively promoted the integration of scientific developments in the pharmaceutical industry. Research by Professor A. M. Khaletsky undoubtedly enriched the theory and practice of organic and pharmaceutical chemistry, made the contribution to the science of the relationships between biological structure and activity, replenished the range of medicine with new drugs and methods of their analysis.&#x0D; The article provides information about the work of one of the oldest departments of the Leningrad Chemical and Pharmaceutical Institute - pharmaceutical chemistry during the leadership of A.M. Khaletsky (1950-x).

Synthesis Characterization and Molinspiration Analysis, Anti-bacterial activity of Novel 2,4,6-tri Substituted Pyrimidines

Published on:June 2022 Journal of Young Pharmacists, 2022; 14(2):174-178 Original Article | doi:10.5530/jyp.2022.14.33 Authors: Sugali Brahmani Bai1, Meka Geethavani2, Chintakunta Ramakrishna2,*1Department of Pharmaceutical Chemistry, Aditya College of Pharmacy, Bengaluru, Karnataka, INDIA.2Department of Pharmaceutical Chemistry, Balaji College of Pharmacy, Ananthapuramu, Andhra Pradesh, INDIA. Abstract: Background: Antibiotics are drugs used to prevent or treat bacterial infections. Globally this antibiotic resistance is rising in high levels and the emergence of new mechanisms to treat infectious diseases. This resistance can be reversed by resistance breakers or a more effective antibiotic against that organism. Antibiotics can save lives but their use contributes to resistant germs. This antibiotic resistance is accelerated when the presence of antibiotics pressure bacteria and fungi to adapt. The three mechanisms of antimicrobial resistance are enzymatic degradation of antibacterial drugs, altering bacterial proteins and changes in membrane permeability to antibiotics. There is a need to synthesise new drugs. Pyrimidines are the most important constituents of nucleic acids to present use in the chemotherapy of AIDS. Methods: Many researchers have attempted to determine the synthetic routes and various biological activities of these compounds. A Variety of 2,4,6-tri substituted Pyrimidines were synthesised by reacting Chalcones with Metformin as one of the guanidine moieties. Results: All the new compounds were characterised by IR, 1H NMR and MASS Spectrometry. The newly prepared compounds were evaluated for their antibacterial action and some of the compounds have shown significant action when compared with Ciprofloxacin as standard in the concentration of 10μg/ml. Conclusion: Among five Synthesized compounds 3 and 4 exhibits significant activity in comparison with that of standard. This is due to the presence of electron-donating groups and predicted molecular descriptors and bioactivity scores using Molinspiration online software and compound 5 showed a good percentage of drug absorption in the gut.Key words: Novel Pyrimidines, Chalcones, Guanidine Moiety, Antibacterial activity, Molinspiration.

Microwave Assisted Synthesis, Characterization and Evaluation for Antimicrobial Activity of Novel 1, 5-Benzothiazepines

Aims: 1,5-benzothiazepine ring is privileged aromatic heterocycles of interest to organic and medicinal chemists because of its ease of synthesis and biological activities. This study aims to synthesize new series of 1, 5-benzothiazepine by direct and efficient microwave assistance and to evaluate for antimicrobial activity by MIC method.&#x0D; Place and Duration of Study: The study was conducted at Department of Pharmaceutical Chemistry, Chebrolu Hanumaiah Institute of Pharmaceutical Sciences, Guntur-19, A. P. from January, 2019 to October, 2021.&#x0D; Methodology: 1, 5-benzothiazepines (BT-21 to BT-40) were synthesized by microwave irradiation. The structures of the products were established by elemental analysis, FTIR, 1H-NMR, 13C-NMR and mass spectroscopic studies. The synthesized compounds were also evaluated for their Antimicrobial activity by MIC method.&#x0D; Results: The microwave assisted synthetic procedure adopted yielded the 1,5-benzothiazepine derivatives BT-21 to BT-40 in good amounts and at a lesser time span. The synthesized 1, 5-benzothiazepine derivatives showed good to moderate antibacterial and antifungal activities.BT-25 having a dihydroxy-methyl-phenyl moiety proved to be more potent against all selected bacterial strains, B. subtilis, S. aureus, E. coli and P. aeruginosa with a MIC value of 64 µg/ml. BT-33 having fluorophenyl moiety, BT-35 having hydroxyl-nitrophenyl moiety and BT-40having dibromophenyl moiety proved to be more potent against all selected fungal strains, A. niger and C. tropicalis with a MIC value of 16 µg/ml.&#x0D; Conclusion: These results showed that the synthesized 1, 5-benzothiazepine derivatives have better scope for further development as antimicrobial agents.

Selective SGLT2 Inhibitor’s Estimation and Validation from Galenical Form by RP-HPLC Method

Aim: The recent analysis is required to do novel and simple, sensitive, precise, efficient, instant and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method for estimation of antidiabetic drug in the unit dosage form. Validation of this method is also planned to make it suitable for the actual use.&#x0D; Study Design: Department of Pharmaceutical Chemistry, Datta Meghe Institute of Medical Sciences deemed to be university, Wardha in collaboration with Balkh university, Mazar-e-sharif, Afghanistan between August 2021 and December 2021.&#x0D; Methodology: In that article develop the method and validate it by estimation of antidiabetic drugs in solid dosage form by RP-HPLC, by using System suitability test, Repeatability, Precision studies (Intra-day and Interday/Intermediate), Linearity/Calibration studies, Robustness, Force degradation, Specificity, Drug recovery/accuracy studies.&#x0D; Results: as per ICH guidelines, the performance if system suitability in remogliflozin were achieved all guidelines; in that, tailing factor (T),separation factors(α),theoretical plates(N),capacity factor (k’), resolution (R) and RSD (%). The validated stress degradation studies under thermal, oxidative, alkali and acid, in few degradation products for remogliflozin (REM).&#x0D; Conclusion: From the results we conclude that, this novel technique which validated for exploration by reverse phase high performance liquid chromatography (RP-HPLC) should be used for routine quality control of remogliflozin (REM) prediction from developed formulation.

"RP-HPLC Method Development and Validation for Estimation of Naftidrofuryl Oxalate Using Box- Behnken Design"

Krishna R Gupta*, Anita R Pounikar and Milind J Umekar Author Affiliations Department of Pharmaceutical Chemistry, Smt Kishoritai Bhoyar College of Pharmacy, India Received: November 26, 2021 | Published: December 07, 2021 Corresponding author: Krishna R Gupta, Professor Department of Pharmaceutical Chemistry, Smt Kishoritai Bhoyar College of Pharmacy, Kamptee, Nagpur (MS), India DOI: 10.26717/BJSTR.2021.40.006447

Comparative Phytochemical Profiling of Garlics (Allium sativum L.) and Onion (Alium cepa L.)

Aims: This research aims to perform the extraction of garlic samples and onion as well as to carry out qualitative phytochemical screening by using Thin Layer Chromatography (TLC) and phytochemical analyses for the detection of carbohydrates, flavonoids, and alkaloids. &#x0D; Study Design: Thin Layer Chromatography (TLC) and some preliminary qualitative phytochemical tests to detect the presence of carbohydrates and reducing sugars, flavonoids, and alkaloids were carried out to compare and identify the chemical compositions in black and white garlic as well as onion.&#x0D; Place and Duration of Study: Pharmaceutical Chemistry Department laboratory, Faculty of Pharmacy Universiti Teknologi MARA Puncak Alam. The whole study was conducted and completed in 12 months.&#x0D; Methodology: Black garlic (BG) is derived from raw white garlic (WG) that is modified by treating it with highly controlled temperature and humidity. In addition to the black and white garlic powder samples, this comparative study was also performed on smoked garlic and onion. The extraction of black garlic (BG), white garlic (WG), crushed smoked garlic (CSG), and onion was performed by using ethanol and ethyl acetate. The comparative phytochemical profiling was conducted by using TLC and qualitative phytochemical analyses were done using standard methods.&#x0D; Results: The presence of amino acid in the ethanolic extracts of black garlic (BG), crushed smoked garlic (CSG), and onion were detected. Ethyl acetate extracts might contain triterpene. Phytochemical screening tests showed the presence of reducing sugars and alkaloids in ethanolic extracts of black garlic (BG), while white garlic (WG) contains flavonoids, alkaloids, and a trace amount of carbohydrates. Both extracts of onion showed the presence of carbohydrate, reducing sugars and flavonoids. Meanwhile alkaloids are only detected in ethanolic extract of onion.&#x0D; Conclusion: The extracts of garlics and onion samples in this study had shown appreciable presence of some important phytochemical compounds in Allium species such as amino acids, flavonoids, alkaloids and carbohydrates.

Theoretical study of the possibility of decamethoxin complexes with thiotriazolinе to be formed

Over the last decade, much attention has been paid to the prevention and treatment of chronic diseases of the oral mucosa.&#x0D; This is primarily due to the increase in the number of patients who seek dental care for diseases of the oral mucosa (DOM).&#x0D; Currently, due to the lack of special epidemiological studies, information on the pathology of the oral mucosa in the literature is almost non-existent. The etiology and pathogenesis have not been definitively elucidated. It is established that a significant role in the pathogenesis of chronic inflammatory processes belongs to the state of the microbiocenosis of the oral mucosa.&#x0D; One of the most striking examples of domestic drugs of the antioxidant group is thiotriazoline, developed by the staff of the NGO “Pharmatron”, as well as specialists of the Department of Pharmaceutical Chemistry of Zaporozhye State Medical University under the leadership of professor Mazur I. A. This drug has an antioxidant and membrane-stabilizing effect.&#x0D; The aim. The combined use of several drugs can be complicated by the formation of their supramolecular complexes. Therefore, before the introduction of several substances in it is necessary to model the possibility of intermolecular interactions between them. For this purpose, methods of quantum chemistry are used.&#x0D; Material and methods. We examined the structures and energy characteristics of the complexes, formed thiotriazoline and decamethoxin, also carried out analysis and established at what temperature the formation of a substance from two active components was possible.&#x0D; Results. As a result, the probability of the formation of intermolecular hydrogen bonds is reduced, which is very well seen in the trend of interactions between acid and morpholine.&#x0D; Conclusions. Quantum chemical study of a two-component system consisting of thiotriazoline and decamethoxine showed that the most energetically advantageous three-component complexes have a sufficiently low interaction energy of thiotriazoline and decamethoxine. In addition, the data that are provided in the analysis of the tree diagram suggest that in the technological process in the manufacture of dosage forms, it is advisable to use a temperature not higher than 115℃.

Molecular Modification: A Tool for Pruning the Molecule

Modern Approaches in Drug Designing Molecular Modification: A Tool for Pruning the Molecule John Sunil R1*, Sridhar V1, Sarbani Pal2 and Jayashree A2 1Department of Pharmaceutical Chemistry, India 2Department of Chemistry, India *Corresponding author: John Sunil R, Department of Pharmaceutical Chemistry, Karimnagar, Telangana, India Submission: July 30, 2021;Published: August 26, 2021 DOI: 10.31031/MADD.2021.03.000565 ISSN: 2576-9170 Volume3 Issue3

Effects of Solvents and Extraction Methods on Naringenin, LC-MS Based Quantitative Studies and Major Metabolite of Cochlospermum gossypium

Aim: The present study was aimed to investigate “Effects of Solvents and Extraction Methods on Naringenin, LC-MS Based Quantitative Studies and major Metabolite of Cochlospermum Gossypium”.&#x0D; Study Design: LC-MS based Quantification Studies.&#x0D; Place and Duration of Study: Department of Pharmaceutical Chemistry, GITAM Institute of Pharmacy, Visakhapatnam, Andhra Pradesh between April 2019 and July 2020.&#x0D; Methodology: Rapid and Quantitative efficient extraction methods were used by using soxhlet and Ultrasound mediated extraction techniques for a range of polar solvents and Isolation of Naringenin by gradient elution technique, In the present study Naringenin was isolated in abundance (1%) by repetitive chromatographic purifications of the ethyl acetate extract of the bark part of C.gossypium. In view of high therapeutic potential, detailed HPLC based quantitative studies was carried out on C. gossypium bark, which contains significant levels of Naringenin.&#x0D; Results: The chromatographic separation was performed on the stationary phase, based on different retention mechanisms due to hydrophilic and hydrophobic interactions between compounds and stationary phase. In total, 24 different polar extracts were generated by soxhlet and ultrasound extraction methods from the two bark samples which were collected from Jaipur town (Telangana state) and Trichur town (Kerala state).&#x0D; Conclusion: The highest Naringenin content (0.59%) was found in the methanol + chloroform (1:1) extract (E8) generated by direct Soxhlet extraction of C.gossypium bark collected from Jaipur (Telangana state).Naringenin was isolated from a non-citrus plant for first time from Cochlospermum gosspyium and more significantly it is isolated in large quantity. It was obtained in 99.9% purity as shown by LCMS.

Analysis of Canagliflozin in Rat Plasma After Oral Administration by Liquid Chromatographic as (Pharmacokinetic Study)

The research work aims to develop a bioanalytical method using liquid chromatography and validated for the determination of canagliflozin by using an internal standard. Department of Pharmaceutical Chemistry, Smt. Kishoritai Bhoyar College of Pharmacy, New Kamptee, Nagpur (MS). Isocratic chromatography separation was achieved on an LC system with PDA detector on an ACE C18 (150mm× 4.6mm × 5µm) column using a mobile phase composition of acetonitrile: ammonium acetate buffer in the ration of 50:50 v/v (pH 4.5), orthophosphoric acid is used to adjust pH of mobile phase and the flow rate at 1.0ml/ min. and estimation was carried out at 291 nm. The retention time of a drug was 4.633 minutes. The method was validated for several parameters (specificity, linearity, precision, and accuracy) and also successfully applied for the pharmacokinetic in female rats. Calibration plot was linear (r2 &gt; 0.9973) over the concentration range of 5-30 µg/ml for canagliflozin. The high recovery and low relative standard deviation (%RSD) confirm the suitability of the method. The result of Limit of Detection (LOD) and Limit of Quantitation (LOQ) were found to be 0.1099 μg/ml and 0.3331 μg/ml, respectively. The new RP-HPLC method can be conveniently adapted for examining canagliflozin concentration in rat plasma after oral administration.

Pharmaceutico-Analytical study of Shadbindu taila

Introduction: Shadbindu taila is an Ayurvedic herbo-mineral preparation used in a variety of clinical conditions especially in diseases of upper part of body. This taila is described as exceptionally beneficial for improving the strength of hands. Before a human trial it is essential to evaluate the quality and authenticity of the trial drug, Standardization is needed for assuring quality of any drug and it should be started with the identification and authentication of the drug Materials and method: Pharmacognostical evaluation carried out at Pharmacognosy department, ITRA, Jamnagar, pharmaceutical and HPTLC study were done at Pharmaceutical chemistry department, ITRA, Jamnagar. Microbiological evaluation was carried out at department of Microbiology I.T.R.A, Jamnagar. Results: Organoleptic examination: Colour: Greenish Brown, Odour: Aromatic, Taste: Salty, Touch: Viscous, oily. Pharmaceutical evaluation: specific gravity 0.9177, density 0.8753 Refractive Index 1 .4860, Acid Value 4.042, Saponification Value 132.43, Iodine Value 169.33 HPTLC at 254nm (short wave) showed mainly 6 major spot and at 366 nm (long wave) showed 2 major spots. No organisms isolated in aerobic and fungal culture, in microbiology evaluation. Conclusion: The trial drug is authentic and devoid of any adulteration. All quality control parameters were found to be within the permissible pharmacopeial limits and the stability and shelf life of the trial drug also will be more as per the pharmaceutical parameters.

Microfluidic Paper-Based Analytical Devices (ΜPADS) in Modern Analysis

Hobbs Bower Reang, Gagandeep and Rohit Bhatia* Author Affiliations Department of Pharmaceutical Chemistry & Analysis, ISF College of Pharmacy, India Received: May 10, 2021 | Published: May 25, 2021 Corresponding author: Rohit Bhatia, Department of Pharmaceutical Chemistry & Analysis, ISF College of Pharmacy, India DOI: 10.26717/BJSTR.2021.36.005794

Scientific substantiation of the concentration of excipients for the manufacture of eye drops Angiolin

Throughout human history, cataracts have been one of the leading causes of blindness. For this disease, we studied the market of drugs of domestic and foreign production.&#x0D; The object of our study was the subgroup S10X Other ophthalmic drugs. Employees of the Department of Pharmaceutical Chemistry of Zaporizhzhia State Medical University (ZSMU) together with specialists of the NGO “Pharmatron” was synthesized a new compound, which was named Angiolin. A rational dosage form in the form of eye drops was proposed for the new drug. Since the drops continue to be the most common and widely used in practice dosage form.&#x0D; We have previously selected the optimal content of the active substance in eye drops. As is known from the technological parameters, eye drops must be isotonic, ie in their composition should be added excipients.&#x0D; The aim of our work is to select the concentration of excipients for the manufacture of eye drops Angiolin.&#x0D; Materials and methods. During the work at the Department of Pharmaceutical Chemistry of ZSMU, three solutions of eye drops Angiolin with different composition were prepared, and later the theoretical osmolarity was calculated.&#x0D; Results. Accurate theoretical calculation of the osmolarity of solutions containing substances with high molecular weight, complex total extracts, and highly concentrated solutions is impossible. Since the excipient was used methylcellulose, it was better to perform such a calculation experimentally, through the determination of osmolality. On the basis of the conducted researches, for correction of osmolarity, we were chosen – sodium chloride. Sodium chloride was selected at a concentration of 7.0 g/l, which creates an osmolality of the drug equal to 234.3 mosmol/kg. The estimated value at the same concentration of sodium chloride was 239.56 mosmol/l. The value of osmolarity of eye drops was calculated from it makes 302,18 mosmol/l that was confirmed the correctness of the chosen concentration of sodium chloride as a part of eye drops.&#x0D; Conclusions. Based on the above, we selected the concentration of excipients for the manufacture of eye drops Angiolin.

A Study on the Inhibitory Potential of Dpp-Iv Enzyme by Lobeline through In silico and In vivo Approaches

Aims: To evaluate the inhibitory activity of Lobeline natural alkaloid against dipeptidyl peptidase IV (DPP IV) enzyme by in silico and in vivo experiments.&#x0D; Study Design: Evaluation of Antidiabetic Activity of Lobeline alkaloid.&#x0D; Place and Duration of Study: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Aleppo University, Aleppo, Syria, between March 2020 and December 2020.&#x0D; Methodology: in silico study was carried out using iGEM docking software to predict the binding affinity of lobeline with DPP IV enzyme in comparison with the reference synthetic compound Sitagliptin. Then in vivo experiment was performed on HFD/alloxan induced diabetic mice to evaluate the anti hyperglycemic effect of lobeline. After treatment duration of 21 days, FBG and the inhibitory effect on DPP IV enzyme activity were measured.&#x0D; Results: Lobeline bound efficiently to the active site of DPP IV enzyme and consumed less binding energy than Sitagliptin. This finding was confirmed by the in vivo study.&#x0D; Administration of lobe line at a dose of 25 mg/kg in HFD/alloxan induced diabetic mice produced a significant reduction in blood glucose level and in DPP IV activity compared to the diabetic control group (P value&gt; .01).&#x0D; Conclusion: Lobe line could be a good candidate to be developed as a natural compound for treating diabetes mellitus.