Aim: The purpose of this study is to investigate the effects of commercial and non-commercial agents used to clean dentures on the surface roughness and hardness of two soft lining materials. Material and Methods: A total of 50 samples were used in this study. Samples were prepared from acrylic resin (Visco Gel) and silicone-based (Molloplast B) soft lining materials. The samples’ roughness and hardness values were measured. After the samples were kept in distilled water, white vinegar, denture cleanser tablet, 2% chlorhexidine gluconate, and 5% sodium hypochlorite for 15 minutes, 30 minutes, 45 minutes, 60 minutes, 2 hours, 8 hours, 24 hours, and 48 hours, roughness and hardness values were measured again. Statistical analysis was performed using the computer program SPSS Statistics 20.0 (SPSS Inc. Chicago, IL, USA) at a 95% confidence interval and p=0.05 significance level. Three-way ANOVA and Tukey’s multiple comparison tests were used for the statistical analysis of data. Results: The analysis of variance determined that the material type and time had a very significant effect (p<.001) on surface roughness and hardness values, while the solutions in which samples were kept had an insignificant effect (p>.05). Conclusion: The surface roughness and hardness of acrylic resin and silicone-based soft lining materials were affected by denture cleaning solutions to different extents.