Surface Of Dental Implants Research Articles

Porous titanium fiber mesh with tailored elasticity and its effect on stromal cells.

Porous titanium fiber mesh (TFM) is considered a suitable scaffold material for bone reconstruction. Also, TFM can be used to cover the surface of bone‐anchored devices, that is, orthopedic or dental implants. The titanium fiber size has an effect of the stiffness as well as porosity of the titanium mesh, which can influence the behavior of bone forming cells. Therefore, the aim of this study was to vary TFM composition, in order to achieve different stiffness, and to assess the effects of such variation on the behavior of bone marrow‐derived stromal cells (BMSCs). With that purpose, nine types of TFM (porosities 60–87%; fiber size 22–50 μm), were examined for their mechanical properties as well as their effect on the proliferation and differentiation of rat bone marrow‐derived stromal cells (rBMSCs) up to 21 days. Dynamic mechanical analysis revealed that the stiffness of TFM were lower than of solid titanium and decreased with larger fiber sizes. The stiffness could effectively be tailored by altering fiber properties, which altered the pore simultaneously. For the 22 and 35 μm size fiber meshes with the highest porosity, the stiffness closely matched the value found in literature for cortical bone. Finally, all tested TFM types supported the growth and differentiation of rBMSCs. We concluded that TFM material has been proven cytocompatible. Further preclinical studies are needed to assess which TFM type is most suitable as clinical use for bone ingrowth and bone regeneration.

Stem-cell-derived ECM sheet-implant complexes for enhancing osseointegration.

Numerous treatment methods have been developed to modify the surface of dental implants to improve cell migration and proliferation, removal torque, and osseointegration. Recent studies have constructed cell sheet-implant complexes with enhanced osteogenic capabilities. However, these complexes have some limitations, such as requirements for complex preparation processes, cell vitality maintenance, strict preservation conditions, and the induction of immunogenicity. Extracellular matrix (ECM) sheets without cells may be a more desirable material. To date, the effect of ECM sheets on implant osseointegration has not been reported. In this study, we fabricated ECM sheet-implant complexes through the combination of rat bone marrow mesenchymal stem cell (BMSC)-derived ECM sheets with sandblasted, large-grit, acid-etched (SLA) implants. These complexes were characterized by light microscopy, scanning electron microscopy (SEM), and immunofluorescence (IF) assays. The adhesion, proliferation, and osteogenic differentiation of BMSCs cultured on ECM sheets were detected in vitro. Then, the ECM sheet-implant complexes were transplanted into the metaphysis of the tibias of rats to evaluate the implant osseointegration in vivo. The results showed that ECM sheets were successfully constructed and showed significantly improved adhesion and proliferation. BMSCs cultured on ECM sheets upregulated the expression levels of the osteogenic-related genes alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (Runx2) compared to controls. In vivo, ECM sheet-implant complexes demonstrated superior new bone formation. Our findings proved that the BMSC-derived ECM sheets promoted osseointegration in vitro and in vivo. The current study indicated that the ECM sheet could be an ideal tissue engineering material, and ECM sheet-implant complexes could provide a strategy with low immunogenicity and easy storage and transportation. This research provides a novel strategy for the development of implant surface modification approaches.

Ultrastructural Characterization of the Titanium Surface Degree IV in Dental Implant Aluminum Free (Acid Attack)

Following the worldwide trend of developing heavy metal free materials, dental implants aren’t out of this tendency. Over the years, a number of techniques to condition the surface of dental implants have been designed and used such as oxide blasting, however the scientific medical community has been concerned about the use of these heavy metals which leads us to investigate and develop new conditioning techniques. The aim of the study was the analysis of the Systhex® implant surface in automatic system for the treatment of the surface with acid, where we can identify the surfaces purity level, pore size, deepness and especially the roughness proportionated by the technology of acid conditioning on the titanium surfaces of degree IV dental implants. We conclude that the automatic conditioning of acid attack promoted cleanliness, homogeneity and ideal roughness for the osseointegration process.

Modifications of Dental Implant Surfaces at the Micro- and Nano-Level for Enhanced Osseointegration.

This review paper describes several recent modification methods for biocompatible titanium dental implant surfaces. The micro-roughened surfaces reviewed in the literature are sandblasted, large-grit, acid-etched, and anodically oxidized. These globally-used surfaces have been clinically investigated, showing survival rates higher than 95%. In the past, dental clinicians believed that eukaryotic cells for osteogenesis did not recognize the changes of the nanostructures of dental implant surfaces. However, research findings have recently shown that osteogenic cells respond to chemical and morphological changes at a nanoscale on the surfaces, including titanium dioxide nanotube arrangements, functional peptide coatings, fluoride treatments, calcium–phosphorus applications, and ultraviolet photofunctionalization. Some of the nano-level modifications have not yet been clinically evaluated. However, these modified dental implant surfaces at the nanoscale have shown excellent in vitro and in vivo results, and thus promising potential future clinical use.

Surface parameters of as-built additive manufactured metal for intraosseous dental implants

Statement of problemTo improve osseointegration, current machined implants are submitted to different surface treatments such as airborne-particle abrasion and acid etching. Although additive manufacturing has allowed the fabrication of implants with custom design, porosity, and roughness, whether good osseointegration can be achieved without subsequent surface treatments is still unclear. PurposeThe purpose of this in vitro study was to investigate the feasibility of using additive manufacturing technology for dental implants without the use of subsequent surface treatments. Material and methodsThe roughness, wettability, and surface energy of a flat test stainless-steel specimen produced from a 3D printer were evaluated. The roughness measurements were obtained by using a mechanical contact profilometer. The wettability was characterized by the sessile drop method by using deionized water and ethylene glycol. The surface energy values were calculated by using the Owens, Wendt, Rabel, and Kaeble (OWRK) computational method. ResultsThe experimental data obtained were Ra=4.55 μm, Rq=5.64 μm, RSm=0.235 mm, Rsk=-0.071, Rku=3.740, Rdq=13 degrees; water contact angle=66 degrees; ethylene glycol contact angle=57 degrees; surface energy=38 mN/m. The measured values were compared with data reported in the literature for commercially available implants. The parameter Ra, which is the most used parameter to describe the surface of dental implants, was 50%, 270%, and 329% higher than that reported in the literature for commercial dental implants. The surface energy was 10% and 19% lower than the representative values in the literature for commercial dental implants. ConclusionsThe results indicate that specimens fabricated by additive manufacturing had higher roughness and lower surface energy than reported results in the literature. Therefore, additive manufacturing was found to produce suitable surface parameters for dental implants, and subsequent surface treatments could be removed from the manufacturing process.

Effects of Mechanical Instrumentation with Commercially Available Instruments Used in Supportive Peri-implant Therapy: An In Vitro Study.

To evaluate topographic changes and effectiveness of mechanical instrumentation upon machined (MA) and roughened (RG) surfaces of dental implants. The coronal one-third of seven RG and seven MA implants was coated with a mixture of cyanoacrylate and toluidine blue dye to resemble calculus. Implants were cleaned with three curettes (SS: stainless steel, PT: plastic, TI: titanium), two ultrasonic tips (UM: metal tip, UP: plastic tip), a titanium brush (TB), and an air-polishing device (AA) until visibly clean. Additionally, a simulation of 1- and 5-year supportive peri-implant therapy (SPT) was performed on 14 implants using the aforementioned instruments with 20 strokes/40 s (T1) or 100 strokes/200 s (T5). Each implant was evaluated using stereomicroscopy, atomic force microscopy, and scanning electron microscopy. UM was the most effective instrument, with 0% average percentage of residual artificial calculus (RAC), followed by TB (2.89%) and UP (4.90%). SS was more effective than TI (15.43% vs 20.12% RAC, respectively), while PT failed to remove any deposit (100% RAC). AA completely removed deposits on RG surfaces but not MA surfaces (26.61% RAC). Noticeable topographic changes were observed between both implant surfaces. RG surfaces became less rough, whereas MA surfaces became rougher at both T1 and T5 with the exception of AA. Plastic- and titanium-like remnants were noted after debridement with PT, SS, and TI, respectively. Artificial calculus removal by mechanical instrumentation, with the exception of PT, was proven to be clinically effective. All instruments induced minor to major topographic changes upon dental implant surfaces. AA did not remarkably change MA and RG surfaces at both micrometer and nanometer levels. Findings from this study may impact the selection of instruments or devices used during SPT protocols.

Restoration of Maxillary Arch Using Smooth Surface Dental Implants in a Case of Aggressive Periodontitis with Established Crohn's Disease: A Special Case File

Restoration of Maxillary Arch Using Smooth Surface Dental Implants in a Case of Aggressive Periodontitis with Established Crohn's Disease: A Special Case File

Influence of Titanium Oxide Pillar Array Nanometric Structures and Ultraviolet Irradiation on the Properties of the Surface of Dental Implants-A Pilot Study.

Aim: Titanium implants are commonly used as replacement therapy for lost teeth and much current research is focusing on the improvement of the chemical and physical properties of their surfaces in order to improve the osseointegration process. TiO2, when it is deposited in the form of pillar array nanometric structures, has photocatalytic properties and wet surface control, which, together with UV irradiation, provide it with superhydrophilic surfaces, which may be of interest for improving cell adhesion on the peri-implant surface. In this article, we address the influence of this type of surface treatment on type IV and type V titanium discs on their surface energy and cell growth on them. Materials and methods: Samples from titanium rods used for making dental implants were used. There were two types of samples: grade IV and grade V. In turn, within each grade, two types of samples were differentiated: untreated and treated with sand blasting and subjected to double acid etching. Synthesis of the film consisting of titanium oxide pillar array structures was carried out using plasma-enhanced chemical vapor deposition equipment. The plasma was generated in a quartz vessel by an external SLAN-1 microwave source with a frequency of 2.45 GHz. Five specimens from each group were used (40 discs in total). On the surfaces to be studied, the following determinations were carried out: (a) X-ray photoelectron spectroscopy, (b) scanning electron microscopy, (c) energy dispersive X-ray spectroscopy, (d) profilometry, (e) contact angle measurement or surface wettability, (f) progression of contact angle on applying ultraviolet irradiation, and (g) a biocompatibility test and cytotoxicity with cell cultures. Results: The application of ultraviolet light decreased the hydrophobicity of all the surfaces studied, although it did so to a greater extent on the surfaces with the studied modification applied, this being more evident in samples manufactured in grade V titanium. In samples made in grade IV titanium, this difference was less evident, and even in the sample manufactured with grade IV and SLA treatment, the application of the nanometric modification of the surface made the surface optically less active. Regarding cell growth, all the surfaces studied, grouped in relation to the presence or not of the nanometric treatment, showed similar growth. Conclusions. Treatment of titanium oxide surfaces with ultraviolet irradiation made them change temporarily into superhydrophilic ones, which confirms that their biocompatibility could be improved in this way, or at least be maintained.

The impact of different dental implant surface properties to osseointegration success of dental implants – A systematic review

Background Osseointegration of dental implants can be considered as the main factor for successful implant stability. This healing process can be related with the surface of dental implants because the surface properties of dental implants affect bone apposition and cell responses. Dental implants have hybrid nature consisting of- implant body which is intrabony part, implant neck which contact with soft tissue and the last part interact with oral environment. So, dental implants have several interfaces and there is a challenge between bacterial colonization and cell functional integration of the dental implant surfaces.. Various surface modifying methods such as blasting, etching, anodizing or coating with biomaterials are introduced. Surface modifications of dental implants is one of the most widely researched fields of implant dentistry. These surface modifications improve the adsorption of protein, cells and so result in faster osseointegration. However, no reports have demonstrated the superiority of one surface treatment over the other. Aim/Hypothesis The purpose of this systematic review was to evaluate the clinical success of different implant surface properties and acquire actual results. Material and Methods An electronic search was conducted in MEDLINE (PubMed search form). The study is based on the Cochrane Review Methods. The main question is ëWhich implant surface modification technique is best in terms of clinical outcomes?’ In last decades (from January 2008 to December 2018), published clinical studies on implants with different surface properties were independently evaluated two reviewers (K.D. and M.H.A.) based on the inclusion criteria. In vitro studies, animal studies, retrospective studies, non-comparative studies and case reports were excluded. (1) Comparative clinical studies of different implant surfaces reporting one year or longer follow-up period, (2) more than ten patients including studies and (3) articles to consider mean marginal bone loss result were included. Results A total of 2604 articles were evaluated from database. 110 articles is repeated, 2,357 articles are animal studies and cell studies, 137 articles not relevant outcomes, 21 clinical studies were evaluated. Three studies excluded because follow-up smaller than 1 year. Remaining 18 studies met the inclusion criteria and included to the review. The modification methods were classified as physically, chemically or biochemically treated. Generally implant failure was related with smoking habit. Because of the low number of studies assessing each surface modification for failure, annual failure rates were not measured by surface modification technique. Implant success were favorable for all modification techniques, but chemically and biochemically modification procedures can improve osseointegration at initial stage. Conclusion and Clinical Implications Within the limitation of this systematic review, it can be concluded that biochemical and chemical techniques can accelerate osseointegration process and may be preferred for early loading planning. However, the studies have different methods and it is difficult to figure out scientifically that superiority of one surface modification technique over another. Further comparative and long term clinical studies are required to identify exact results about the surface modification techniques.

Electron Microscopy of the Surface of Dental Implants and Metal-Containing Nanoparticles Obtained in Supernatants

The surface of two dental implant systems, “Nobel Biocare” and “Alpha BiO”, and metal-containing nanoparticles, isolated from the tissues surrounding dental implants, has been investigated. The implant surface structure, the elemental and phase composition of particles, and their arrangement in the granulation tissue have been studied by X-ray tomography, transmission and scanning electron microscopy, z-contrast scanning transmission microscopy, electron diffraction, and energy-dispersive mapping, using microscopes Quanta 200-3D, FEI Тechnai Osiris at an accelerating voltage of 200 kV, and an X-ray microtomograph TOMAC. An analysis of the relief indicates that the emission of nanoparticles from the “Alpha BiO” implant surface to the adjacent tissues is more likely than from the “Nobel Biocare” implant surface. The particles of micrometer and submicrometer sizes of “Nobel Biocare” implants are found to consist mainly of titanium dioxide of both modifications, rutile and anatase, whereas in the case of “Alpha BiO” implants, along with titanium dioxide and titanium nitride, there are aluminum oxides in the particle composition. The elemental composition of nanoparticles is more diverse; it includes Fe, Ca, Na, Cl, S, Si, P, etc. It is revealed that microbial contamination does not always play the leading role in the suppression of previously obtained osteointegration.

Evaluation of dental implants surface after exposure to corrosion agents

Evaluation of dental implants surface after exposure to corrosion agents

What are the long-term survival and complication rates of complete-arch fixed implant rehabilitation in edentulous patients?

Cohort selection The study cohort was selected through screening of electronic records of all patients rehabilitated with interforaminal implants and implant-supported fixed complete dental prostheses (IFCDPs) over an 11-year period at the Tufts University School of Dental Medicine. Evaluation was based on medical and dental history; clinical oral examination of hard and soft tissues; and radiographic examination during a single visit.Data analysis Survival and failure rates of implants and prostheses were recorded based on predefined criteria. Along with descriptive statistics, the observed annual incidence, and the estimated five- and ten-year biologic and technical complications were computed with 95% confidence intervals.Results The study cohort included 41 patients with an average age of 65.8 years (range = 39 to 88 years) and comprised 19 females and 22 males. A total of 359 moderately rough surface dental implants (Nobel Biocare, Straumann, Biomet 3i) were used to rehabilitate 36 cement-retained and 19 screw-retained metal-ceramic IFCDPs in maxilla (N = 32) mandible (N = 23). The mean observation times for implants and prostheses were 5 and 7.5 years, respectively. Two implant failures in a single patient were recorded 11 years post-insertion, yielding an implant survival rate of 99.4%. The cumulative prostheses survival rate was 98.2% (100% at five years and 92.9% at ten years). Biologic and/or technical complications were associated with all 55 prostheses. Among major complications, the most frequent biologic complication was peri-implantitis while porcelain fractures were the most common technical complication. The cumulative rates of 'prostheses free of biologic complications' were 50.4% (95% CI: 36.4% to 63.0%) at five years and 10.1% (95% CI: 3.5% to 20.8%) at ten years. The cumulative rates for 'prosthesis free of technical complications' were 56.4% (95% CI: 41.7% to 68.8%) at five years and 9.8% (95% CI: 3.2% to 21.0%) at ten years.Conclusions Metal-ceramic implant-supported fixed full arch dental prostheses show high survival rates at five-year follow-up, including an implant survival rate of 99.4% and prosthesis survival rate of 98.2%. However biological and technical complications were observed in 47.1% and 42%, respectively.

Investigation of silver nanoparticles on titanium surface created by ion implantation technology.

Objectives: Using dental Ti implants has become a well-accepted and used method for replacing missing dentition. It has become evident that in many cases peri-implant inflammation develops. The objective was to create and evaluate the antibacterial effect of silver nanoparticle (Ag-NP) coated Ti surfaces that can help to prevent such processes if applied on the surface of dental implants.Methods: Annealing I, Ag ion implantation by the beam of an Electron Cyclotron Resonance Ion Source (ECRIS), Ag Physical Vapor Deposition (PVD), Annealing II procedures were used, respectively, to create a safely anchored Ag-NP layer on 1x1 cm2 Grade 2 titanium samples. The antibacterial effect was evaluated by culturing Staphylococcus aureus (ATCC 29213) on the surfaces of the samples for 8 hours, and comparing the results to that of glass as control and of pure titanium samples. Alamar Blue assay was carried out to check cytotoxicity.Results: It was proved that silver nanoparticles were present on the treated surfaces. The average diameter of the particles was 58 nm, with a 25 nm deviation and Gaussian distribution, the the filling factor was 25%. Antibacterial evaluation revealed that the nanoparticle covered samples had an antibacterial effect of 64.6% that was statistically significant. Tests also proved that the nanoparticles are safely anchored to the titanium surface and are not cytotoxic.Conclusion: Creating a silver nanoparticle layer can be an option to add antibacterial features to the implant surface and to help in the prevention of peri-implant inflammatory processes. Recent studies demonstrated that silver nanoparticles can induce pathology in mammal cells, thus safe fixation of the particles is essential to prevent them from getting into the circulation.

Antimicrobial efficacy of copper-doped titanium surfaces for dental implants.

The aim of this in vitro study was to quantify the antibacterial effect of a copper-deposited titanium surface as a model for dental implants on the peri-implantitis-associated strain Porphyromonas gingivalis (DSM 20709). A spark-assisted anodization method in a combined deposition-anodization process was applied to deposit copper on discs made of titanium. This method allows the deposition of different concentrations of copper on the surface by varying the process time. Conventional culturing was used to investigate the adhesion of P. gingivalis onto the discs over 2, 4, and 6 h as well as to study the antibacterial effect of copper released in solution. The viability of the bacterial cells is strongly inhibited on copper-deposited discs and reaches a CFU reduction of 3 log-units after 6 h in comparison to the reference. The copper released in solution causes a reduction of 4 log-units after a 6 h incubation time. With a 6 h incubation time, the CFU count decreases with increasing copper concentrations on the disc (by 2% for the 1.3 µg/disc; 32% for the 5.6 µg/disc; and 34% for the 9.5 µg/disc). However, at a higher copper concentration of 17.7 µg/disc, after 6 h, the decrease in the CFU count is less pronounced than that observed in solution, where a further decrease is observed. In conclusion, copper-functionalized titanium significantly reduces the survival of adhered bacteria and decreases the viable bacterial count in the environment surrounding the titanium. Thus, the area surrounding implants is being protected by copper released from the surface, forming a "safe zone" for improved implant healing.

The Effect of Er,Cr:YSGG and Diode Laser Applications on Dental Implant Surfaces Contaminated with Acinetobacter Baumannii and Pseudomonas Aeruginosa.

Treatment of peri-implantitis through several implant surface decontamination techniques have been reported, however, some of them can negatively alter the implant surface or enhance more bacterial resistance. The aim of this in vitro study was to evaluate implant surface decontamination by means of Er,Cr:YSGG and diode lasers. Fifty micro-textured (MTX) dental implants were contaminated with Acinetobacter baumannii (n = 25) and with Pseudomonas aeruginosa (n = 25). All implants were then divided into five groups for the decontamination procedure. In group I (GI), decontamination was done with an Er,Cr:YSGG laser (2780 nm), while in group II (GII) decontamination was performed using photodynamic therapy (a 650 nm diode laser). In Group III (GIII) decontamination was performed with photodynamic therapy (an 808 nm diode laser), and in group IV (GIV) decontamination was performed with 0.12% chlorhexidine. Group V (GV) was the control group with no decontamination. After decontamination, colony forming units (CFU) were counted and implants were prepared for SEM analysis. A significant difference (p < 0.001) was observed for GI compared to the other groups, and also for GIV compared to both GII and GIII. The Er,Cr:YSGG laser (GI) showed the best results in decontaminating the implant surface. Chlorhexidine (GIV), proved to be better in decontaminating the implant surface than photodynamic therapy GII and diode laser GIII. No significant difference was found between group GII and GIII. The SEM analysis showed no significant change in the implant surface topography. The results of this study suggest that the Er,Cr:YSGG laser can be considered as an effective technique for reducing bacteria contamination on implant surfaces.

Metal Nanoparticles Released from Dental Implant Surfaces: Potential Contribution to Chronic Inflammation and Peri-Implant Bone Loss.

Peri-implantitis is an inflammatory disease affecting tissues surrounding dental implants. Although it represents a common complication of dental implant treatments, the underlying mechanisms have not yet been fully described. The aim of this study is to identify the role of titanium nanoparticles released form the implants on the chronic inflammation and bone lysis in the surrounding tissue. We analyzed the in vitro effect of titanium (Ti) particle exposure on mesenchymal stem cells (MSCs) and fibroblasts (FU), evaluating cell proliferation by MTT test and the generation of reactive oxygen species (ROS). Subsequently, in vivo analysis of peri-implant Ti particle distribution, histological, and molecular analyses were performed. Ti particles led to a time-dependent decrease in cell viability and increase in ROS production in both MSCs and FU. Tissue analyses revealed presence of oxidative stress, high extracellular and intracellular Ti levels and imbalanced bone turnover. High expression of ZFP467 and the presence of adipose-like tissue suggested dysregulation of the MSC population; alterations in vessel morphology were identified. The results suggest that Ti particles may induce the production of high ROS levels, recruiting abnormal quantity of neutrophils able to produce high level of metalloproteinase. This induces the degradation of collagen fibers. These events may influence MSC commitment, with an imbalance of bone regeneration.

In Vitro Evaluation of Bacterial Adhesion and Bacterial Viability of Streptococcus mutans, Streptococcus sanguinis, and Porphyromonas gingivalis on the Abutment Surface of Titanium and Zirconium Dental Implants.

Objective To evaluate the in vitro adherence and viability of 3 bacterial species Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), and Porphyromonas gingivalis (ATCC 33277) on the surfaces of dental implants of titanium, zirconium, and their respective fixing screws. Methods Two analysis groups were formed: group 1 with 3 titanium pillars and group 2 with 3 zirconium pillars, each with their respective fixing screws. Each of these groups was included in tubes with bacterial cultures of Streptococcus mutans (ATCC 25175), Streptococcus sanguinis (ATCC 10556), and Porphyromonas gingivalis (ATCC 33277). These samples were incubated at 37°C under anaerobic conditions. Bacterial adherence was assessed by measurement of the change in colony-forming units (CFU), and bacterial viability was evaluated with the colorimetric test of 3-(4,5-dimethylthiazol-2)-2,5 diphenyl tetrazolium bromide (MTT). Results The bacterial adhesion in the titanium abutments was higher for Streptococcus mutans (190.90 CFU/mL), and the viability was greater in Porphyromonas gingivalis (73.22%). The zirconium abutment group showed the highest adherence with Streptococcus mutans (331.82 CFU/mL) and the highest bacterial viability with the S. sanguinis strain (38.42%). The titanium fixation screws showed the highest adhesion with S. sanguinis (132.5 CFU/mL) compared to the zirconium fixation screws where S. mutans had the highest adhesion (145.5 CFU/mL). The bacterial viability of S. mutans was greater both in the titanium fixation screws and in the zirconium fixation screws 78.04% and 57.38%, respectively. Conclusions Our results indicate that there is in vitro bacterial adherence and viability in both titanium abutments and zirconium abutments and fixation screws for both. Streptococcus mutans is the microorganism that shows the greatest adherence to the surfaces of both titanium and zirconium and the fixing screws of the latter. On the contrary, bacterial viability is greater on the titanium abutments with P. gingivalis than on the zirconium abutments with S. sanguinis. With respect to the fixation screws, in both cases, the viability of S. mutans was greater with respect to the other bacteria. In general, the titanium abutments showed less adherence but greater bacterial viability.

Surface roughness of biomaterials and process parameters of titanium dioxide gritblasting for productivity enhancement

Titanium is used in dental implants because of its properties as a biomaterial. Surface roughness is very important and fundamental for obtaining required results of biocompatibility. Dental implants with moderately rough surfaces are commonly used in the treatment of edentulous patients. Aiming for improvements in productivity, experiments were conducted in a dental implant industry to evaluate the influence of machining parameters on the surface of dental implants. Samples of different diameters were machined with specific parameters and their roughnesses were measured, before and after blasting, with and without asepsis chemical attack. The results indicate a random increase in roughness according to increase of feed parameter, evidencing that there is no relation between roughness and machining feed after gritblasting. Samples exposed to gritblasting that did not undergo chemical asepsis showed a decrease in roughness, compared to roughness of blasted samples and submitted to asepsis process. However, this trend remained constant leading to the conclusion that titanium-blasting process makes the surface roughness uniform, independently of first roughness variation. No relationship was observed between the variation of feed and the variation of roughness. Thus, in terms of productivity, cutting feed was increased with no harm to the biocompatibility. After more than twenty years of its first use in the industry, this study proves the possibility of changes in the process parameters of titanium dioxide gritblasting to increase productivity in the manufacture of dental implants.

Very low-quality evidence suggests that there are no differences in implant failure and bone loss between machined and rough surface dental implants in patients with periodontitis

Very low-quality evidence suggests that there are no differences in implant failure and bone loss between machined and rough surface dental implants in patients with periodontitis

Release of VEGF from Dental Implant Surface (IML® Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations

This study aimed to evaluate the combined use of the Concentrated Growth Factor (CGF) and the liquid phase of CGF (LPCGF) on dental implant surfaces, using a medical device to determine the migration of growth factors, from the implant surface to the recipient. The implants were permeated by autologous growth factors, using a specific centrifuge device. CGF adhesion on the implant surface was evaluated through a scanning electron microscope analysis. To assess the release of the vascular endothelial growth factor (VEGF) from CGF, LPCGF, and CGF- or LPCGF-permeated implant, an ELISA assay was carried out. The results showed that the concentration of the growth factor VEGF was greater in CGF than in LPCGF. Our innovative technique allowed the incorporation of autologous growth factors on the surface of the dental implants. Moreover, we reported the release of VEGF, over time, by CGF- or LPCGF-permeated implant. On this basis, it was possible to obtain a biologically active implant surface, essential to create intercellular communication and neo-angiogenesis, to facilitate wound healing and tissue regeneration.