Material For Dental Implants Research Articles

Investigation of mechanical properties and long-term efficacy of chitosan-reinforced bamboo and nano bio-silica-reinforced composite materials for dental implants

This work is dedicated to fabricated Chitosan-reinforced bamboo and nano bio-silica (1%, 2%, and 3% by volume) using the hand-layup method and investigate their mechanical properties. Mechanical characterization of samples proves that 2% volume nano bio silica-reinforced composites (C2) exhibit the highest tensile, flexural, and compressive strengths. Finite element analysis of dental implants for static and dynamic loading (60 cycles per minute) also confirmed the suitability of the C2 composites for dental implant applications, with Von-Mises stresses below the yield strength in human jaw bones. These findings highlight the potential use of bio-composite material for the dental implants in future.

Enhanced Longevity and Biocompatibility in Modern Dental Implant Materials: A Comprehensive Review

This review encompasses the innovative progress in dental implant materials that has been witnessed over the last decade. A meticulous analysis of the diverse range of materials including metals, ceramics, polymers, and composites is undertaken to discern their efficacy and improvements in terms of biocompatibility, durability, and overall performance. The objective is to evaluate and synthesize the prevailing knowledge, focusing on the enhanced characteristics that these materials impart to dental implants, ensuring optimal integration and longevity.

A chlorogenic acid-chitosan complex bifunctional coating for improving osteogenesis differentiation and bactericidal properties of zirconia implants.

Poor osteogenesis caused by limited bioactivity and peri-implantitis caused by bacterial colonization are the main challenges affecting the use of zirconia-based materials in dental implants. Accordingly, the development of a surface treatment method with an antibacterial effect and that promotes osteogenesis without damage to cells is crucial for yttrium-stabilized tetragonal zirconia (Y-TZP) implants. Herein, we have developed a functional surface modification strategy whereby a poly (ethylene imine)/hyaluronic acid /chitosan-chlorogenic acid (PEI/HA/CGA-CS) conjugate is deposited on a zirconia surface by the layer-by-layer (LBL) technique, enhancing its osteogenic differentiation and antibacterial activities. The results showed that the PEI/HA/CGA-CS coating improved the wettability of the zirconia surface and maintained stable release of CGA. The PEI/HA/CGA-CS functional coating was found to promote early cell adhesion, proliferation, differentiation, and calcification. The results of bacterial adhesion and activity tests showed that the coating effectively inhibits the proliferation and activity of Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) without impairing the biological activity of osteoblasts. In addition, we found that the PEI/HA/CGA-CS coating enhances the osteogenesis of MC3T3-E1 cells by promoting the protein expression of Nephronectin (NPNT) and activating the Wnt/β-catenin signaling pathway. The above results are of profound significance for the practical application of zirconia-based implants. DATA AVAILABILITY: Data will be made available on request.

Biomechanical behavior of carbon fiber-reinforced polyetheretherketone as a dental implant material in implant-supported overdenture under mandibular trauma: A finite element analysis study.

Implant-supported overdentures are well-known and widely accepted treatment modality to increase retention which is a crucial factor for determining patient satisfaction. The placement of two implants in the anterior region can be selected as a first-line treatment in patients with the atrophic mandibular ridge. The purpose of this research was to assess the biomechanical effects of carbon fiber-reinforced polyetheretherketone (CFR-PEEK) implant-supported overdenture in the event of 2,000 N forefront trauma to an atrophic edentulous mandible by using the finite element analysis method. Three types of mandible models were simulated; the first one was an edentulous atrophic mandible model; in the second model, 3.5 × 11.5 mm CFR-PEEK implants; and in the third model, 4.3 × 11.5 mm CFR-PEEK implants were positioned in the region of the lateral incisor of the identical edentulous atrophic mandible. Maximum Von Misses stresses 979.261 MPa, 1,454.69 MPa, and 1,940.71 MPa and maximum principal stresses 1,112.74 MPa, 1,249.88 MPa, and 1,251.33 MPa have been detected at the condylar neck area and minimum principal stresses - 1,203.38 MPa, -1,503.21 MPa, and - 1,990.34 MPa have been recorded at the symphysis and corpus regions from M1 to M3, respectively. In addition, the M2 and M3 models showed low-stress distributions around the implant-bone interface, particularly where the implants were in contact with cancellous bone. The results showed that the insertion of different diameters of CFR-PEEK implants led to low and homogenous stress distribution all around the implant-bone interface and stresses transferred directly to the condylar neck areas. Therefore, it was observed that CRF-PEEK implants did not change the basic behavior of the mandibula in response to frontal stresses.

A Comprehensive Review of Surface Coatings for enhancement of Anti-Microbial and Anti-Corrosive Properties of Titanium Implant Surface

Dental implants have revolutionized the field of restorative dentistry, providing a durable and long-lasting solution for replacing missing teeth. Among various biomaterials, titanium has proven to be the most suitable material for dental implants because of its incredible biocompatibility, mechanical properties, and unique surface characteristics. This scientific write-up reviews the comprehensive overview of various surface modification techniques employed to improve the osseointegration, antimicrobial properties, surface topography, and anti-corrosion behavior of titanium as a dental implant biomaterial, highlighting its role in improving implant success rates and long-term clinical outcomes

Agglomeration effect on biomechanical performance of CNT-reinforced dental implant using micromechanics-based approach

Dental implants have long played an important role in restoring lost teeth, but there are still concerns about their durability and long-term success. Commercial dental implants have traditionally been made of metallic and ceramic materials like titanium and zirconia; however, each kind of material has restrictions regarding osseointegration and mechanical characteristics that differ between native bone and the implant material, limiting the implant's longevity and reliability. To address these concerns, this research explores the use of carbon nanotubes (CNTs) in restorative dentistry, their excellent properties make them an ideal candidate for promoting bone growth around implanted device and ensuring long-lasting success. The objective of this study was to understand how CNT properties when incorporated into the titanium matrix may be able to better adapt to the oral environment taking into consideration the CNT agglomeration effects when designing reinforced nanocomposite materials for dental implant. A mathematical formulation of the micromechanics model was developed and improved to extend its application for the case of CNT-based composite materials for dental implants. A three dimensional (3D) model of bone structure around the osseointegrated dental implant was established considering different compositions of implant material. Finite Element Analysis (FEA) were conducted to assess the aggregation effect of implant incorporating CNTs into the titanium matrix, considering CNTs with both spherical inclusions (CNT clusters), and randomly dispersive ones (CNTs) in the titanium matrix, on osseointegration and bone remodeling around the dental implant and supporting bone system over a period of 48 months. Firstly, the effects of CNT-Ti implantation on time-dependent performance are evaluated in a computational remodeling framework. Then, Von Mises equivalent stresses are investigated to evaluate the stress distributions and micromotions in jaw bones of loaded implant with different composition of prosthetic material. Three agglomeration patterns are considered, particularly without agglomeration (ζ = ξ), partial and complete agglomeration (ζ < ξ, ξ = 1). Further, the influence of CNTs volume fraction variation is taken into account to predict the mechanical response of the bony system after CNT-reinforced dental implantation. It can be inferred that the agglomeration of CNTs reduces the elastic stiffness of the matrix. This is due to the fact that when CNTs are agglomerated, the inter-tube contacts are reduced and the effective stiffness of the matrix is decreased.

Color Formation on Titanium Surface Treated by Anodization and the Surface Characteristics: A Review

Tooth loss is a common problem found in human beings. The challenge in replacing teeth is to restore the natural tooth appearance. Dental implants have overcome this challenge. Titanium (Ti) is an attractive material for dental implants due to its excellent properties and biocompatibility, but the grayish appearance is a concern. A process of supplying the current voltage to an electrochemical cell forming the oxide layer on metal is called anodization. Anodization is a surface modification technique that creates micro- and nano-roughness and an oxide layer on the Ti surface, improving the properties of dental implants. The higher the voltage applied to the anodizing process, the higher the anodic film thickness forms. The different thicknesses of titanium oxide (TiO2) film cause variations in refractive index and reflective index, resulting in a desired color on the Ti surface. The most useful colors for dental application have been gold (10 V), yellow (60 to 65 V), and pink (70 to 80 V) in hue. Yellow and gold hues were mostly used in restoration or abutment areas while pink hue was used for gingival areas. The purposes of this literature review are to assess the current knowledge on the anodization process of titanium and its characteristics and to provide an overview of the literature on color formation on Ti surfaces by anodization.

A Comprehensive Review on Manufacturing and Characterization of Polyetheretherketone Polymers for Dental Implant Applications.

Aging, tooth trauma, and pathological infections cause partial or total tooth loss, leading to the usage of dental implants for restoration treatments. As such, mechanical and tribological properties play an important role in the osseointegration and durability of these implants. Metallic and ceramic implants are shown to have mechanical properties much higher than the natural teeth structure, leading to stress shielding-related failure of an implant. Stress shielding occurs due to the difference in the elastic modulus between the implant material and the surrounding teeth structure, leading to bone loss and implant failure. The implant's properties (i.e., mechanical) should be as close as human teeth components. To achieve this, various materials and coatings are being developed and investigated. This review is a comprehensive survey of materials, manufacturing, coating techniques, and mechanical and tribological characterizations of dental implants, with a particular focus on polyetheretherketone (PEEK) as a potential alternative dental implant material. PEEK has mechanical properties similar to natural teeth, which make it a promising material for dental implants. The findings of this review suggest that PEEK offers superior biocompatibility, osseointegration, and wear resistance for implant applications. With the help of bioactive coatings, bone growth on the implant surface can be promoted. In addition, PEEK dental implants made using three-dimensional (3D) printing technology can significantly reduce the cost of implants, making them more affordable and increasing access to dental care, which can improve oral health significantly. In summary, this review highlights the potential of PEEK as a promising alternative dental implant material, and provides an overview of various techniques, testing, and future directions for PEEK dental implants.

Bioactive Glass Modified with Zirconium Incorporation for Dental Implant Applications: Fabrication, Structural, Electrical, and Biological Analysis.

Implantology is crucial for restoring aesthetics and masticatory function in oral rehabilitation. Despite its advantages, certain issues, such as bacterial infection, may still arise that hinder osseointegration and result in implant rejection. This work aims to address these challenges by developing a biomaterial for dental implant coating based on 45S5 Bioglass® modified by zirconium insertion. The structural characterization of the glasses, by XRD, showed that the introduction of zirconium in the Bioglass network at a concentration higher than 2 mol% promotes phase separation, with crystal phase formation. Impedance spectroscopy was used, in the frequency range of 102-106 Hz and the temperature range of 200-400 K, to investigate the electrical properties of these Bioglasses, due to their ability to store electrical charges and therefore enhance the osseointegration capacity. The electrical study showed that the presence of crystal phases, in the glass ceramic with 8 mol% of zirconium, led to a significant increase in conductivity. In terms of biological properties, the Bioglasses exhibited an antibacterial effect against Gram-positive and Gram-negative bacteria and did not show cytotoxicity for the Saos-2 cell line at extract concentrations up to 25 mg/mL. Furthermore, the results of the bioactivity test revealed that within 24 h, a CaP-rich layer began to form on the surface of all the samples. According to our results, the incorporation of 2 mol% of ZrO2 into the Bioglass significantly improves its potential as a coating material for dental implants, enhancing both its antibacterial and osteointegration properties.

In Vitro Study of Zirconia Surface Modification for Dental Implants by Atomic Layer Deposition.

Zirconia is a promising material for dental implants; however, an appropriate surface modification procedure has not yet been identified. Atomic layer deposition (ALD) is a nanotechnology that deposits thin films of metal oxides or metals on materials. The aim of this study was to deposit thin films of titanium dioxide (TiO2), aluminum oxide (Al2O3), silicon dioxide (SiO2), and zinc oxide (ZnO) on zirconia disks (ZR-Ti, ZR-Al, ZR-Si, and ZR-Zn, respectively) using ALD and evaluate the cell proliferation abilities of mouse fibroblasts (L929) and mouse osteoblastic cells (MC3T3-E1) on each sample. Zirconia disks (ZR; diameter 10 mm) were fabricated using a computer-aided design/computer-aided manufacturing system. Following the ALD of TiO2, Al2O3, SiO2, or ZnO thin film, the thin-film thickness, elemental distribution, contact angle, adhesion strength, and elemental elution were determined. The L929 and MC3T3-E1 cell proliferation and morphologies on each sample were observed on days 1, 3, and 5 (L929) and days 1, 4, and 7 (MC3T3-E1). The ZR-Ti, ZR-Al, ZR-Si, and ZR-Zn thin-film thicknesses were 41.97, 42.36, 62.50, and 61.11 nm, respectively, and their average adhesion strengths were 163.5, 140.9, 157.3, and 161.6 mN, respectively. The contact angle on ZR-Si was significantly lower than that on all the other specimens. The eluted Zr, Ti, and Al amounts were below the detection limits, whereas the total Si and Zn elution amounts over two weeks were 0.019 and 0.695 ppm, respectively. For both L929 and MC3T3-E1, the cell numbers increased over time on ZR, ZR-Ti, ZR-Al, and ZR-Si. Particularly, cell proliferation in ZR-Ti exceeded that in the other samples. These results suggest that ALD application to zirconia, particularly for TiO2 deposition, could be a new surface modification procedure for zirconia dental implants.

Multiscale Evaluation of Jaw Geometry Reproduction Obtained Via the Use of Selected Orthodontic Materials in Dental Implants and Orthodontics—In Vitro Case Study

In this paper, the multiscale analysis of the reproduction accuracy of jaw geometry obtained via the use of selected orthodontic materials is discussed. Impressions were made from two types of impression material. An accuracy assessment of the model geometry mapping was performed using noncontact systems, including a fringe projection optical 3D scanner, computed tomography, and a focus variation microscope. Measurements were made in three modes for comparison, as were the silicone and polyether impression materials. These modes were a jaw model and impression, an impression and plaster model, and plaster and jaw models. The research results are presented as colorful maps of deviations. Data analysis showed that deviations were the smallest in the case of silicone and that the best fit occurred between the silicone impression and the plaster model. The conducted research confirmed the validity of the assumptions considering the use of multiscale analysis for geometric analysis. The use of modern multiscale measurement methods allows for shorter and more efficient prosthetic operations. At present, these devices are expensive and complicated to use, but developments in technology should simplify the process, and prosthetic professionals should be aware of the possibilities described in the paper.

ОСОБЕННОСТИ И ОТЛИЧИЯ ФОРМИРОВАНИЯ БИОПЛЕНКИ В ОБЛАСТИ КЕРАМИЧЕСКИХ И ТИТАНОВЫХ ИМПЛАНТАТОВ

The subject of the study is the formation of a biofilm in the area of ceramic and titanium implants.&#x0D; Objectives. A systematic analysis of modern domestic and foreign literature sources to determine the main features and differences in the formation of a biofilm in the field of ceramic and titanium implants.&#x0D; Methodology. The study was based on the search and study of original articles on dental implantation in databases: Russian State Library, eLibrary, PubMed, The Cochrane Library, Google Scholar. The main selection of materials was carried out by keywords.&#x0D; Results. Rehabilitation of edentulous spaces in patients with osseointegrated dental implants is a scientifically recognized and well-documented treatment method. Bacterial adhesion on dental implants can cause inflammatory conditions such as mucositis and peri-implantitis. Peri-implantitis can lead to bone resorption and eventual loss of the implant. It has been established that zirconium oxide has a lower bacterial capacity for adhesion and inflammatory infiltration compared to titanium, and due to its hygienic properties, it has significant advantages in maintaining the level of soft tissues of the aesthetic zone and maintaining the level of the alveolar bone. Numerous studies show that the clinical use of zirconium oxide implants is practically not accompanied by manifestations of peri-implantitis, and gum epithelial tissues are able to firmly and reliably attach to their surface. Zirconia is bioinert to other materials found in the oral cavity and is particularly suitable for patients who are allergic or intolerant to metals. The latest techniques and materials, which include zirconium dioxide, have important advantages, and its use as a material for dental implants significantly increases the clinical effectiveness of treatment.&#x0D; Conclusions. A review of the literature on the formation of a biofilm in the area of dental implants showed that zirconium oxide has a lower bacterial ability for adhesion and inflammatory infiltration compared to titanium, and due to hygienic properties has significant advantages in maintaining the level of soft tissues of the aesthetic zone and maintaining the level of the alveolar bone.

Effects of surface topography through laser texturing on the surface characteristics of zirconia-based dental materials: surface hydrophobicity, antibacterial behavior, and cellular response

The continuous need for high-performance implants that provide significant biological properties has led to extensive research into the topographic patterns of bioceramics in recent years. Their excellent aesthetics, biocompatibility, low plaque affinity, and ability to reproduce a natural-looking appearance have contributed to their success in dentistry. 3 mol% Yttria-stabilized zirconia (3YSZ) is gaining popularity as a material for dental implants due to its excellent mechanical properties and minimal degradation when exposed to body temperature. However, such materials show limited biological and antibacterial performance for dental applications. The purpose of this work was to develop microtopographies on the surface of 3YSZ ceramic by laser ablation technique, in order to improve its biological response and antibacterial behaviors. Two types of microtextures, including micro-grooves and micro-channels geometries were fabricated onto the zirconia ceramics using the laser ablation technique. The effects of different microtextures on the wettability, biological and antibacterial behaviors of 3YSZ ceramics were studied. The results indicate that all of the microstructure patterns are capable of improving the performance of 3YSZ. Wettability is a decisive factor that determines the antibacterial performance of textured zirconia ceramics. The microtextured surfaces all display hydrophobic behavior, thus yielding an effective improvement of antibacterial performance for 3YSZ ceramics. Cell-surface interactions were assessed for 7 days on both zirconia textured surfaces and a nontextured control with pre-osteoblast MC3T3-E1 cells. The obtained results showed the positive influence of textured zirconia surfaces on cell biological response.

Laser Functionalization: An Emerging Modality to Enhance the Osseointegration Potential of Polyetheretherketone as a Dental Implant Material

The unique physical and mechanical attributes of polyetheretherketone (PEEK) have lent significant momentum to the motion that PEEK can be used as a material of choice for dental implants. However, in contrast to titanium or zirconia, PEEK has very limited inherent osteoconductive properties. A wide variety of research has been conducted till date to improve the bioactivity of PEEK surface. Varied methods have been proposed, laser surface treatment of PEEK being one of the promising techniques. Data collection was limited to the last 10 years from 2012 to 2021. Eleven articles were found suitable for this review found in the databases of PubMed and Google Scholar. Considerable alterations have been observed in surface roughness and wettability characteristics of PEEK after laser surface treatment, which improved the osseointegration property of PEEK to be utilized as a dental implant material. The current paper is aimed at reviewing different types of laser applications to the PEEK surfaces and its effect on bone-to-PEEK implant contact.

Shrinkage Volume, Compressive Strength, and Surface Roughness Y-TTRIA Stabilized Tetragonal Zirconia Polycrystal (Y-TZP) Using Binders Variation PVA:PEG as an Alternative Dental Implants Materials.

Yttria-stabilized tetragonal zirconia polycrystal (Y-TZP) is one of the materials that can be used as an alternative material for dental implants because of its good mechanical, biocompatible, and aesthetic properties. The binder used for ceramic processing to help bond is polyvinyl alcohol (PVA), which can increase the density of the ceramic material, and polyethylene glycol (PEG), which is used as a plasticizer for PVA, so it is pretty soft when pressed. The sample was divided into five groups for volume shrinkage and compressive strength examination consisting of K1 (PVA 100%), K2 (PEG 100%), P1 (PVA:PEG 95:5), P2 (PVA:PEG 90:10), and P3 (PVA:PEG 85:15) and four groups on the surface roughness test, namely, K (PVA:PEG 1%), P1 (PVA:PEG 2%), P2 (PVA:PEG 3%), and P3 (PVA:PEG 4%). PVA:PEG binder with various concentrations was mixed with Y-TZP. The mixture was pressed using a uniaxial pressing method and continued by sintering at 1200°C for 4 hours. Least significant difference (LSD) test result showed that there was a significant difference in the compressive strength value and shrinkage volume between groups K1 and K2 and P3, and groups K2 with P1, P2, and P3. Post hoc LSD surface roughness test showed a significant difference between groups K with P2 and P3 and P1 and P3 (p < 0.05). There were no significant differences (p > 0.05) between K with P1 and P2 with P3. The Y-TZP group with the PVA binder mixture had the highest compressive strength, while the highest volume shrinkage was found in the PEG group. The next highest compressive strength and volume shrinkage values were found in the PVA:PEG group with a ratio of 95:5, 102.44 MPa, and 12.5%. The best PVA:PEG ratio of 95:5 is used to make surface roughness measurement samples. The best results showed that mixing Y-TZP with 4% PVA:PEG binder had the highest surface roughness compared to other PVA:PEG binders, namely 1.3450 μm. From this study, it can be concluded that the best PVA:PEG percentage ratio to produce volume shrinkage and compressive strength is 95:5. The higher the concentration of PVA:PEG (95:5) binder mixed with Y-TZP, the higher the porosity will be.

Polyether ether ketones (PEEK): Properties and applications as implants for alternative dentistry materials: A narrative review

Aim: Titanium has been a popular material for dental implants. However, there are several drawbacks to these materials, including the occurrence of allergies, bone resorption, surface damage, contamination linked to peri-implantitis, high modulus of elasticity, and less aesthetically pleasing hue. As a result, polyether ether ketone (PEEK) can be utilized as a substitute material in dentistry. The purpose of this review of the literature is to identify the attributes and uses of PEEK in dentistry, particularly for implant applications. Materials and Methods: The articles were independently evaluated during the screening procedures in accordance with the eligibility requirements. The criteria also include studies that are written in English and published on PubMed, ScienceDirect, ResearchGate, and Web of Science, as well as narrative reviews, research articles, and other studies. Articles published before 2014, case reports, case series, book chapters, and articles not related to PEEK in dentistry were excluded in the criteria. Strategy to find articles using PICO includes patients in needs of dental implants, PEEK, titanium, and PEEK as an alternative material for dental implant. The risk of bias was assessed using Cochrane Risk of Bias Tool 2 (RoB 2). Results: A total of 26 articles were identified, of which 16 were of particular relevance to the use of PEEK in dental applications, especially implants. Conclusion: Due to its low elastic modulus, good mechanical structures, and bone-contact biocompatibility when used as a bulk implant, PEEK can be a substitute material in oral implantology. It is possible to improve PEEK’s bone-contact biocompatibility and lessen its downsides by altering the material’s surface and mixing.

Osteoblast Response of Additively Manufactured Zirconia and Alumina-Toughened Zirconia.

Zirconia ceramics have been widely used in dentistry. Herein, we assess the surface morphology, surface texture, and osteoblast response of additively manufactured zirconia and alumina-toughened zirconia (ATZ) in comparison with titanium. The surface roughness, contact angle, and surface microstructure of titanium sandblasted with large-grit alumina and subsequently acid-etched using 18% HCl and 49% H2SO4 (SLA-titanium), uniaxially pressed zirconia (UP zirconia), additively manufactured zirconia (AM zirconia), and additively manufactured ATZ (AM ATZ) were investigated. Moreover, the cell viability, alkaline phosphatase (ALP) activity, and gene expression of type I collagen on these materials were evaluated. The data were statistically analyzed using one-way ANOVA with Tukey's post hoc test. SLA-titanium showed the highest surface roughness and contact angle. The other three materials showed comparable surface roughness and contact angles. Micro- and nanoroughness were observed on the surface of SLA-titanium. UP zirconia and AM zirconia had similar surface morphologies. The cell viability, ALP activity, and gene expression of type I collagen on AM zirconia were comparable to or better than those on SLA-titanium. Our results indicate that AM zirconia is a promising material for zirconia dental implants.

Osseointegration behavior of carbon fiber reinforced polyetheretherketone composites modified with amino groups: An in vivo study.

Polyetheretherketone (PEEK) has become increasingly popular in dentistry and orthopedics due to its excellent chemical stability, reliable biosafety, and low elastic modulus. However, PEEK's biomechanical strength and bioactivity are limited and need to be increased as an implant material. The previous study in vitro has shown that the amino-functionalized carbon fiber reinforced PEEK (A-30%-CPEEK) possessed enhanced mechanical property and bioactivity. This study aims to evaluate the effect of amino groups modification on the osseointegration behavior of carbon fiber reinforced PEEK (30%-CPEEK) in rabbits. Herein, 30%-CPEEK and A-30%-CPEEK implant discs were implanted in rabbit skulls for 5 weeks, with pure titanium implants serving as a control. The bone-forming ability and osseointegration in vivo were systematically investigated by micro-computed tomography analysis, scanning electron microscope observation, and histological evaluation. Our results showed that all detection parameters were significantly different between the A-30%-CPEEK and 30%-CPEEK groups, favoring those in the A-30%-CPEEK, whose appraisal parameters were equal to or better than pure titanium. Therefore, this study supported the importance of amino groups in facilitating the new bone formation and bone-implant integration, suggesting that A-30%-CPEEK with enhanced osseointegration will be a promising material for dental or orthopedic implants.

Dissolution Behavior of Hydrothermally Treated Hydroxyapatite-Titanium Nitride Films Coated on PEEK: In Vitro Study.

Polyetheretherketone (PEEK) has become an alternative material for orthopaedics and dental implants. However, bio-inertness is an important limitation in this material. In the present study, a hydroxyapatite (HA)–titanium nitride (TiN) coating was fabricated via pulsed DC magnetron sputtering and treated with hydrothermal treatment to improve the bioactive property of PEEK. The dissolution behavior of the coating was studied in simulated body fluid solution (SBF) at 1, 3, 5, 7, 14, 21, 28, and 56 days. The coating surface was analyzed before and after the immersion process by X-ray photoelectron spectroscopy (XPS), atomic force microscope (AFM), and scanning electron microscope (SEM). The calcium and phosphorus concentration alteration in SBF was quantified by an inductively coupled plasma-optical emission spectrometer (ICP-OES). Coating dissolution and the precipitation of calcium phosphate complex from SBF were observed as occurring suddenly and continuously throughout the immersion times. These processes resulted in an alteration in both physical and chemical coating properties. After 56 days, the coating remained on PEEK surfaces and the Ca/P ratio was 1.16. These results indicate that HA-TiN coating via pulsed DC magnetron sputtering followed by hydrothermal treatment improved the bioactivity of materials and provided a potential benefit to orthopedics and dental applications.

Effects of Carbonate Hydroxyapatite (CHA) on the Development of Heart and Cranium Cartilage of Zebrafish (Danio rerio Hamilton, 1882) Larvae

Abstract One of the most commonly utilized materials for dental implants is carbonate hydroxyapatite (CHA). However, its usage must be free of tissue toxicity. Therefore, this study aimed to investigate how the use of CHA as a dental implant material affected the development of zebrafish (Danio rerio) embryos. CHA treatment was administered to 3–3.5 hours to 72 hours post-fertilization (hpf) embryos. Alterations in embryo and larva shape were all studied to determine toxicity. Several abnormalities that might arise during development were also examined by monitoring morphological alterations in embryos and larvae. Furthermore, heart morphology in larvae aged 72 hpf; heart rate in embryos aged 24, 48, and 72 hpf; and cardiac histology structure in larvae aged 30 dpf were all used to study heart development. Alizarin Red and Alcian Blue (ARAB) staining was employed to determine the anatomy of the cranial cartilage in larvae aged 6 dpf. Data were statistical analysis with SPSS ver. 21 and the significance was determined using one-way analysis of variance (ANOVA). The morphological examination results revealed that the embryo and larvae had no morphological abnormalities. Furthermore, the heart was developing normally, according to examinations of morphology, histology, and heart rate. The cranial cartilage had no flaws, the structure was complete, and the length and angle of the cranial cartilage did not change between the control and CHA treatments. Overall, CHA exposure did not affect the development of zebrafish (Danio rerio) embryos, according to the findings. Keywords: CHA, Cranium, Embryo, Heart, Toxicity, Zebrafish