Deep Relationships Research Articles

Old specimens for old branches: Assessing effects of sample age in resolving a rapid Neotropical radiation of squirrels

Ultraconserved Elements (UCEs) have been useful to resolve challenging phylogenies of non-model clades, unpuzzling long-conflicted relationships in key branches of the Tree of Life at both deep and shallow levels. UCEs are often reliably recovered from historical samples, unlocking a vast number of preserved natural history specimens for analysis. However, the extent to which sample age and preservation method impact UCE recovery as well as downstream inferences remains unclear. Furthermore, there is an ongoing debate on how to curate, filter, and properly analyze UCE data when locus recovery is uneven across sample age and quality. In the present study we address these questions with an empirical dataset composed of over 3800 UCE loci from 219 historical and modern samples of Sciuridae, a globally distributed and ecologically important family of rodents. We provide a genome-scale phylogeny of two squirrel subfamilies (Sciurillinae and Sciurinae: Sciurini) and investigate their placement within Sciuridae. For historical specimens, recovery of UCE loci and mean length per locus were inversely related to sample age; deeper sequencing improved the number of UCE loci recovered but not locus length. Most of our phylogenetic inferences—performed on six datasets with alternative data-filtering strategies, and using three distinct optimality criteria—resulted in distinct topologies. Datasets containing more loci (40% and 50% taxa representativeness matrices) yielded more concordant topologies and higher support values than strictly filtered datasets (60% matrices) particularly with IQ-Tree and SVDquartets, while filtering based on information content provided better topological resolution for inferences with the coalescent gene-tree based approach in ASTRAL-III. We resolved deep relationships in Sciuridae (including among the five currently recognized subfamilies) and relationships among the deepest branches of Sciurini, but conflicting relationships remain at both genus- and species-levels for the rapid Neotropical tree squirrel radiation. Our results suggest that phylogenomic consensus can be difficult and heavily influenced by the age of available samples and the filtering steps used to optimize dataset properties.

Identification and Early Warning of Financial Fraud Risk Based on Bidirectional Long-Short Term Memory Model

In modern market economy, corporate financial frauds emerge one after another, which have a huge impact on the stock market and triggered an unprecedented credit crisis. Therefore, it is particularly important to identify financial frauds. Improving the efficiency, accuracy, and coverage of fraud identification in financial reports through digital and intelligent means is one of the important links to improve credit risk control of securities companies and also for securities companies to accurately price related financial products of target companies. Traditional recognition methods based on artificial rules can cover relatively limited indicators; rule parameters are set randomly. Besides, it is difficult to make rules based on high-dimension indicators and to dig the hidden deep relationships between indicators. This paper summarizes the relevant indicators of financial fraud identification from the perspective of financial and nonfinancial characteristics. Then, the identification and early warning of financial fraud risk based on bidirectional long-short term memory model are proposed. This method uses the idea of ensemble learning, weights the probability of financial key indicators, and uses the optimal transfer probability to solve the financial fraud risk results. The results show that the industry-specific modeling can significantly improve the accuracy of the financial fraud identification model and it also can effectively help the government regulatory departments, investors, and audit departments to correctly identify the financial fraud of listed companies.

New insights into the phylogeny of the complex thalloid liverworts (Marchantiopsida) based on chloroplast genomes.

Marchantiopsida (complex thalloid liverworts) are one of the earliest lineages of embryophytes (land plants), and well-known for their air pores and chambers, pegged rhizoids, and absence of organellular RNA editing sites. Despite their importance to an understanding of early embryophyte evolution, many key nodes within this class remain poorly resolved, owing to the paucity of genetic loci previously available for phylogenetic analyses. Here, we sequenced 54 plastomes, representing 28 genera, nearly all families, and all orders of Marchantiopsida. Based on these plastomes, we present a hypothesis of deep relationships within the class, and make the first investigations of gene contents and synteny. Overall, the Marchantiopsida plastomes were well-conserved, with the exception of the genus Cyathodium that has plastomes with higher GC content, fewer single sequence repeats (SSRs), and more structural variations, implying that this genus might possess RNA editing sites. Abundant repetitive elements and six highly divergent regions were identified as suitable for future infrafamilial taxonomic studies. The phylogenetic topology of Sphaerocarpales, Neohodgsoniales and Blasiales within Marchantiopsida was essentially congruent with previous studies but generally we obtained higher support values. Based on molecular evidence and previous morphological studies, we include Lunulariales in Marchantiales and suggest the retention of narrowed delimitation of monotypic families. The phylogenetic relationships within Marchantiales were better resolved, and 13 monophyletic families were recovered. Our analyses confirmed that the loss of intron 2 of ycf3 is a synapomorphy of Marchantiidae. Finally, we propose a new genus, Asterellopsis (Aytoniaceae), and present an updated classification of Marchantiopsida. The highly supported phylogenetic backbone provided here establishes a framework for future comparative and evolutionary studies of the complex thalloid liverworts.

Relationships at Work: Integrating the Perspectives of Disability Partners to Enhance a Peer Navigation Intervention.

ObjectiveThe Our Peers-Empowerment and Navigational Support (OP-ENS) community-based participatory research study developed, implemented, and evaluated a peer navigator intervention aimed at improving health and healthcare access among Medicaid beneficiaries with disabilities. Peer navigators are community partners with physical disabilities trained to deliver structured peer support interventions that can address barriers to care. The purpose of this paper is two-fold. First, it explicates the relational work the peer navigators do with peers in delivering the intervention. Second, it illustrates how our community-based participatory approach empowered peer navigators to share their knowledge to refine the intervention.MethodsClinical coordinator team meeting notes, critical incident documentation reports, peer navigator reflections (n = 20) were analyzed thematically to understand the peer navigators' relational work and intervention refinements. Following Labov's 6-stage approach to personal narratives and a collaborative writing process academic, clinical, and disability partners co-wrote descriptive exemplars to showcase these processes.FindingsThrough the manualized OP-ENS intervention process, peer navigators helped peers achieve incremental successes. Peer navigators used their training and personal experiences to engage with peers and forge deep connections and relationships of trust. As a result, peers identified a wide-range of social health concerns, including poverty, social isolation, and racial and disability related discrimination that might otherwise go unaddressed. True to the principles of community-based participatory research, by fostering an equity-focused collaboration and listening to peer navigators, the project team implemented subtle but salient refinements to the intervention. Refinements included an explicit focus on social determinants of health affecting peers' health and wellbeing and supplemental trainings to help peer navigators support peers with significant mental health needs.ConclusionThe peer navigators were intentional and skilled at relationship building, thus complex elements which impact peers' health were addressed. Peer navigators were empowered to communicate their perspectives with the study team, who worked together to strengthen the intervention processes and infrastructure. This atmosphere of trust and collaboration amongst diverse stakeholders was instrumental to OP-ENS' successful implementation. Healthcare systems should consider implementing peer support interventions that are responsive to consumer input to address social determinants of health for persons with disabilities.

New insights on the evolutionary relationships between the major lineages of Amoebozoa

The supergroup Amoebozoa unites a wide diversity of amoeboid organisms and encompasses enigmatic lineages that have been recalcitrant to modern phylogenetics. Deep divergences, taxonomic placement of some key taxa and character evolution in the group largely remain poorly elucidated or controversial. We surveyed available Amoebozoa genomes and transcriptomes to mine conserved putative single copy genes, which were used to enrich gene sampling and generate the largest supermatrix in the group to date; encompassing 824 genes, including gene sequences not previously analyzed. We recovered a well-resolved and supported tree of Amoebozoa, revealing novel deep level relationships and resolving placement of enigmatic lineages congruent with morphological data. In our analysis the deepest branching group is Tubulinea. A recent proposed major clade Tevosa, uniting Evosea and Tubulinea, is not supported. Based on the new phylogenetic tree, paleoecological and paleontological data as well as data on the biology of presently living amoebozoans, we hypothesize that the evolution of Amoebozoa probably was driven by adaptive responses to a changing environment, where successful survival and predation resulted from a capacity to disrupt and graze on microbial mats-a dominant ecosystem of the mid-Proterozoic period of the Earth history.

ON THE POLITICS OF UNTRANSLATABILITY: A STUDY OF "THE RELUCTANT FUNDAMENTALIST" AND ITS URDU TRANSLATION "BUNYAD PARAST

It is a fact that Translatability and Untranslatability have been disputed by various scholars over a long period and the debate goes ahead to the present time. The translation is such a delicate and intricate undertaking, that it raises some major concerns to deal with, therefore, this paper examines numerous issues related to the translation of source text into the target text. As is known that the translation process is a difficult task, hence to deal with both the apparent and deep relationships of language, a translator should have some critical linguistics expertise to avoid ambiguity in the translated version of a source text. Translating one language into another language faces a bunch of problems including style, syntax, vocabulary, semantics, and grammar. The present paper focuses on the semantic problems between the source text and its translated version. Here those words which create ambiguity between the source text and its translation version are investigated. For this purpose, Apter and Catford‘s concept of untranslatability has been used for the analysis of data. The findings show that there are numerous issues related to semantics that create a tension of meaning in both texts. These include variations between the source and target texts at the lexical and syntactic levels.

Phylogenomic Coalescent Analyses of Avian Retroelements Infer Zero-Length Branches at the Base of Neoaves, Emergent Support for Controversial Clades, and Ancient Introgressive Hybridization in Afroaves.

Retroelement insertions (RIs) are low-homoplasy characters that are ideal data for addressing deep evolutionary radiations, where gene tree reconstruction errors can severely hinder phylogenetic inference with DNA and protein sequence data. Phylogenomic studies of Neoaves, a large clade of birds (>9000 species) that first diversified near the Cretaceous–Paleogene boundary, have yielded an array of robustly supported, contradictory relationships among deep lineages. Here, we reanalyzed a large RI matrix for birds using recently proposed quartet-based coalescent methods that enable inference of large species trees including branch lengths in coalescent units, clade-support, statistical tests for gene flow, and combined analysis with DNA-sequence-based gene trees. Genome-scale coalescent analyses revealed extremely short branches at the base of Neoaves, meager branch support, and limited congruence with previous work at the most challenging nodes. Despite widespread topological conflicts with DNA-sequence-based trees, combined analyses of RIs with thousands of gene trees show emergent support for multiple higher-level clades (Columbea, Passerea, Columbimorphae, Otidimorphae, Phaethoquornithes). RIs express asymmetrical support for deep relationships within the subclade Afroaves that hints at ancient gene flow involving the owl lineage (Strigiformes). Because DNA-sequence data are challenged by gene tree-reconstruction error, analysis of RIs represents one approach for improving gene tree-based methods when divergences are deep, internodes are short, terminal branches are long, and introgressive hybridization further confounds species–tree inference.

City Symphony

City Symphony

Discovering Factors that Influence Physician Scientist Success in Academic Medical Centers.

This study investigated factors influencing success of physician scientists in Academic Medical Centers. These organizations and individuals drive healthcare in the United States. Translation of scientific discovery to medical practice moves at an astoundingly slow and ineffective rate. We must understand what contributes to physician scientist success to speed up translation. Through a lens of dialectic process theory, a grounded theory approach identified emergent factors from lived experiences of 31 individuals, at various experience levels, with MD and PhD degrees. Role balance, autonomy, organizational support, teamwork, life-cycle mentorship, and relational capacity were relevant factors impacting success. Role balance was important for success. Teamwork, organizational support, and life-cycle mentorship helped individuals grow, achieve balance, and respect, but relational capacity emerged as a critical driver for realizing both individual and organizational success. One person cannot execute these complex roles on their own, but development of deep and meaningful relationships through teamwork, collaboration, and life-cycle mentorship are essential for life satisfaction and success.

Scots and France As Seen through Alba Amicorum, 1540s-1720s1

The article will study the Scots found in alba amicorum from the 1540s through to the 1720s along socio-professional lines. The relationships developed by these Scottish contributors with the album owners are usually hard to decipher at the personal level within the strict confines of album entries. Yet certain findings emerge to appreciate and delineate the contours of these contacts which evolved according to the identity of these individuals themselves but also their messages, environments, religious persuasions, gender, status, and social interactions to name but a few. The very notion of relating or rather that of friendship, inherent in these livres d’amitié through their name, is stretched to its limits both chronologically speaking and in terms of intensity, dealing with both deep and superficial relationships. The collation of documents seems to be one promising avenue to investigate that nature of the relationships thus formed or cultivated. Actually, the issue of relating within alba seems to be too restrictively construed and needs to be opened up. These album entries firmly ground these Scots within France and its various milieus, whether student, professional, clerical, diplomatic, or scientific and highlight their relations with these environments and activities or professions.

Resolving deep evolutionary relationships within the RNA virus phylum Lenarviricota.

The RNA virus phylum Lenarviricota is composed of the fungi-associated families Narnaviridae and Mitoviridae, the RNA bacteriophage Leviviridae, and the plant and fungi-associated Botourmiaviridae. Members of the Lenarviricota are abundant in most environments and boast remarkable phylogenetic and genomic diversity. As this phylum includes both RNA bacteriophage and fungi- and plant-associated species, the Lenarviricota likely mark a major evolutionary transition between those RNA viruses associated with prokaryotes and eukaryotes. Despite the remarkable expansion of this phylum following metagenomic studies, the phylogenetic relationships among the families within the Lenarviricota remain uncertain. Utilising a large data set of relevant viral sequences, we performed phylogenetic and genomic analyses to resolve the complex evolutionary history within this phylum and identify patterns in the evolution of virus genome organisation. Despite limitations reflecting very high levels of sequence diversity, our phylogenetic analyses suggest that the Leviviridae comprise the basal lineage within the Lenarviricota. Our phylogenetic results also support the construction of a new virus family—the Narliviridae—comprising a set of diverse and phylogenetically distinct species, including a number of uniquely encapsidated viruses. We propose a taxonomic restructuring within the Lenarviricota to better reflect the phylogenetic relationships documented here, with the Botourmiaviridae and Narliviridae combined into the order Ourlivirales, the Narnaviridae remaining in the order Wolframvirales, and these orders combined into the single class, the Amabiliviricetes. In sum, this study provides insights into the complex evolutionary relationships among the diverse families that make up the Lenarviricota.

Психогігієнічні принпипи використання здоров’язберігаючих технологій у сучасних закладах вищої освіти

Objective: We established and scientifically substantiated the psychohygienic principles of using health-preserving technologies in modern institutions of higher education. Materials and methods. The research was conducted on the basis of National Pyrohov Memorial Medical University. A well-structured set of highly informative modern hygienic, medical-sociological, psychophysiological and psychodiagnostic methods and methods of statistical analysis were used to adequately achieve this goal. Results. The data obtained in the course of research indicate that as conceptual psychohygienic principles of effective use of health-preserving technologies in modern institutions of higher education should be noted: (1) regime-adaptive principle of application of psychohygienic components in the structure of modern health-preserving technologies, which determines the need to take into account the main regime elements of educational and extracurricular activities of students in specific conditions of the educational process, determining and constantly monitoring the level of educational adaptation and academic performance of students, especially in professionally oriented disciplines; (2) psychophysiological principle of application of psychohygienic components in the structure of modern health technologies, which is determined by the level of development of leading socially and professionally significant psychophysiological functions of the body of girls and boys; (3) personal principle of application of psychohygienic components in the structure of modern health technologies, which is related to the peculiarities of assessing the processes of formation of leading personality traits of student youth, which play an important role under the influence of both favorable and, above all, unfavorable educational factors. extracurricular activities; (4) integrative-functional principle of application of psychohygienic components in the structure of modern health technologies, which is determined by the presence of deep relationships between individual components of the functional state and adaptive capacity of young girls and young boys, which have a complex impact on health and theoreticalу- and, mainly, practically-significant components of students’ training. Conclusions. In the course of the research the leading psychohygienic principles of using health-preserving technologies in modern modern institutions of higher education have been identified and scientifically substantiated.

Three-Dimensional Fault Model and Activity in the Arc-Shaped Tectonic Belt in the Northeastern Margin of the Tibetan Plateau

The arc-shaped tectonic belt, located in the northeastern margin of the Tibetan Plateau, is one of the leading edges of the plateau’s outward growth and uplift expansion, with a large number of active faults and frequent seismic activity. Researchers have carried out numerous studies on active faults in this region, and a wealth of reliable basic data has been accumulated. However, integrating multidisciplinary data to establish a 3D geometrical structure model that is concerned about seismogenic tectonics and can be tested, has become the key to restricting the regional seismic hazard evaluation. Based on a series of published active tectonic research, we analyze in detail the surface and deep coupling relationships of the major active faults in this region and establish three sets of 3D fault structure models, which are built respectively by active fault mapping and dip angles (the V1 model), 7 magnetotelluric profiles and 7 auxiliary profiles (the V2 model), and multi-source data (the V3 model) and continuously close to the real geological facts. From the model perspective, it is suggested that the controversial Haiyuan fault is a crustal-scale left-slip fault and the shape of the Liupanshan fault reflects the absorption of the left-slip component of the Haiyuan fault. Comparing the same fault plane of these three models, we find that the V3 model is more consistent with geological facts, showing that by assisting the multi-source data 3D geological modeling technique we can establish a 3D geological model closest to the real regional structure. Finally, combining the V3 model, the fault segmentation, and the empirical formulas of the moment magnitude-rupture parameters, we segment the faults and calculate the potential moment magnitudes of the major active faults in the study region. The faults with relatively higher seismic hazards are the Liupanshan fault, the southeastern segment of the Xiangshan-Tianjingshan fault, the westernmost segment of the Haiyuan fault, and the West Qinling fault, of which the estimated potential moment magnitudes are generally more than 7.0. Our study provides a referenced 3D geological model for exploring the deep structures of the region, regional geological research, and earthquake disaster prevention.

Infrared and visible image fusion based on cross-modal extraction strategy

Infrared and visible images can characterize the same scene by diverse modalities. However, most of existing deep learning-based fusion methods extract the two source image features by the dual independent extraction networks, which leads to the loss of deep feature relationships between source images. To this end, we propose a novel infrared and visible image fusion method based on cross-modal extraction strategy (CMEVIF), which could build an interactional relationship between dual independent extraction networks to help the model extract enough deep features. Specifically, we design two feature extraction networks to extract two kinds of cross-modal feature maps, i.e., background maps and target maps, which contain the main information of one source image and the additional information of another source image. Subsequently, all these cross-modal feature maps are utilized to reconstruct the fusion image. Besides, we design two region-based loss functions to guide the network to focus on specific regions of source images. Ablation experiments demonstrate the effectiveness of cross-modal extraction strategy and region-based loss functions. Compared with typical fusion methods, experimental results demonstrate the effectiveness and superiority of our method in both objective metrics and visual impressions.

How many single-copy orthologous genes from whole genomes reveal deep gastropod relationships?

The Gastropoda contains 80% of existing mollusks and is the most diverse animal class second only to the Insecta. However, the deep phylogeny of gastropods has been controversial for a long time. Especially the position of Patellogastropoda is a major uncertainty. Morphology and some mitochondria studies concluded that Patellogastropoda is likely to be sister to all other gastropods (Orthogastropoda hypothesis), while transcriptomic and other mitogenomic studies indicated that Patellogastropoda and Vetigastropoda are sister taxa (Psilogastropoda). With the release of high-quality genomes, orthologous genes can be better identified and serve as powerful candidates for phylogenetic analysis. The question is, given the current limitations on the taxon sampling side, how many markers are needed to provide robust results. Here, we identified single-copy orthologous genes (SOGs) from 14 gastropods species with whole genomes available which cover five main gastropod subclasses. We generated different datasets from 395 to 1610 SOGs by allowing species missing in different levels. We constructed gene trees of each SOG, and inferred species trees from different collections of gene trees. We found as the number of SOGs increased, the inferred topology changed from Patellogastropoda being sister to all other gastropods to Patellogastropoda being sister to Vetigastropoda + Neomphalina (Psilogastropoda s.l.), with considerable support. Our study thus rejects the Orthogastropoda concept showing that the selection of the representative species and use of sufficient informative sites greatly influence the analysis of deep gastropod phylogeny.

Mitochondrial phylogenomics and mitogenome organization in the parasitoid wasp family Braconidae (Hymenoptera: Ichneumonoidea)

BackgroundMitochondrial (mt) nucleotide sequence data has been by far the most common tool employed to investigate evolutionary relationships. While often considered to be more useful for shallow evolutionary scales, mt genomes have been increasingly shown also to contain valuable phylogenetic information about deep relationships. Further, mt genome organization provides another important source of phylogenetic information and gene reorganizations which are known to be relatively frequent within the insect order Hymenoptera. Here we used a dense taxon sampling comprising 148 mt genomes (132 newly generated) collectively representing members of most of the currently recognised subfamilies of the parasitoid wasp family Braconidae, which is one of the largest radiations of hymenopterans. We employed this data to investigate the evolutionary relationships within the family and to assess the phylogenetic informativeness of previously known and newly discovered mt gene rearrangements.ResultsMost subfamilial relationships and their composition obtained were similar to those recovered in a previous phylogenomic study, such as the restoration of Trachypetinae and the recognition of Apozyginae and Proteropinae as valid braconid subfamilies. We confirmed and detected phylogenetic signal in previously known as well as novel mt gene rearrangements, including mt rearrangements within the cyclostome subfamilies Doryctinae and Rogadinae.ConclusionsOur results showed that both the mt genome DNA sequence data and gene organization contain valuable phylogenetic signal to elucidate the evolution within Braconidae at different taxonomic levels. This study serves as a basis for further investigation of mt gene rearrangements at different taxonomic scales within the family.

VOIP technology in grassroots politics: Transforming political culture and practice?

This article investigates how the adoption and use of digital technologies shape political culture and practice in grassroots political groups, particularly focusing on how VOIP technologies enable and/or constrain groups to work across physical space and form political relationships among participants. While this article is grounded in a case study of one broad-based coalition in Sydney, Australia, the findings expand our understanding of how digital technology shapes political culture and practice in grassroots spaces by (a) analysing an organisation both before and after the adoption of VOIP technology and (b) focusing on a case study where the organisation attempted to maintain rather than transform their political culture and practice with the adoption of new digital organising methods. The article argues that the instrumental benefits of digital technologies come at a cost: VOIP technologies may constrain the formation of deep relationships and flatten distinctive political practices within grassroots political organisations.

Phylogenomics of the Aphididae: Deep relationships between subfamilies clouded by gene tree discordance, introgression and the gene tree anomaly zone

AbstractAphids (Hemiptera: Aphididae) are a lineage of ~5200 plant‐feeding insects most abundant in temperate regions. The diversification of aphids is thought to be a rapid radiation, whereas abiotic and biotic factors heavily influence the morphologies. These factors have clouded the taxonomy at all taxonomic ranks, and the effect can be viewed in many incongruent molecular and morphological phylogenies. In this study, we address this problem using both genome and transcriptome data to estimate the phylogenomic relationships between 12 subfamilies with 48 ingroup taxa. We predicted a novel well‐curated dataset of phylogenetically consistent orthologues that included 3162 genes to estimate a concatenated maximum likelihood and multi‐species coalescent species trees. Our results suggest that there are three main clades of Aphididae subfamilies, which are congruent with a previous Sanger sequencing‐based phylogenetic study. However, the relationship between the three clades of subfamilies is clouded by gene tree discordance, introgression and parent–child branches along the backbone that fall within the gene tree anomaly zone. In addition, our results suggest an introgression event between two agriculturally important species of aphids within the subfamily Aphidinae. Our research provides the first phylogenomic study of the Aphididae subfamilies and a foundation for future molecular and morphological studies into this adaptive radiation of insects.

Towards the plastome evolution and phylogeny of Cycas L. (Cycadaceae): molecular-morphology discordance and gene tree space analysis

BackgroundPlastid genomes (plastomes) present great potential in resolving multiscale phylogenetic relationship but few studies have focused on the influence of genetic characteristics of plastid genes, such as genetic variation and phylogenetic discordance, in resolving the phylogeny within a lineage. Here we examine plastome characteristics of Cycas L., the most diverse genus among extant cycads, and investigate the deep phylogenetic relationships within Cycas by sampling 47 plastomes representing all major clades from six sections.ResultsAll Cycas plastomes shared consistent gene content and structure with only one gene loss detected in Philippine species C. wadei. Three novel plastome regions (psbA-matK, trnN-ndhF, chlL-trnN) were identified as containing the highest nucleotide variability. Molecular evolutionary analysis showed most of the plastid protein-coding genes have been under purifying selection except ndhB. Phylogenomic analyses that alternatively included concatenated and coalescent methods, both identified four clades but with conflicting topologies at shallow nodes. Specifically, we found three species-rich Cycas sections, namely Stangerioides, Indosinenses and Cycas, were not or only weakly supported as monophyly based on plastomic phylogeny. Tree space analyses based on different tree-inference methods both revealed three gene clusters, of which the cluster with moderate genetic properties showed the best congruence with the favored phylogeny.ConclusionsOur exploration in plastomic data for Cycas supports the idea that plastid protein-coding genes may exhibit discordance in phylogenetic signals. The incongruence between molecular phylogeny and morphological classification reported here may largely be attributed to the uniparental attribute of plastid, which cannot offer sufficient information to resolve the phylogeny. Contrasting to a previous consensus that genes with longer sequences and a higher proportion of variances are superior for phylogeny reconstruction, our result implies that the most effective phylogenetic signals could come from loci that own moderate variation, GC content, sequence length, and underwent modest selection.

Overly shallow?: Miscalibrated expectations create a barrier to deeper conversation.

People may want deep and meaningful relationships with others, but may also be reluctant to engage in the deep and meaningful conversations with strangers that could create those relationships. We hypothesized that people systematically underestimate how caring and interested distant strangers are in one's own intimate revelations and that these miscalibrated expectations create a psychological barrier to deeper conversations. As predicted, conversations between strangers felt less awkward, and created more connectedness and happiness, than the participants themselves expected (Experiments 1a-5). Participants were especially prone to overestimate how awkward deep conversations would be compared with shallow conversations (Experiments 2-5). Notably, they also felt more connected to deep conversation partners than shallow conversation partners after having both types of conversations (Experiments 6a-b). Systematic differences between expectations and experiences arose because participants expected others to care less about their disclosures in conversation than others actually did (Experiments 1a, 1b, 4a, 4b, 5, and 6a). As a result, participants more accurately predicted the outcomes of their conversations when speaking with close friends, family, or partners whose care and interest is more clearly known (Experiment 5). Miscalibrated expectations about others matter because they guide decisions about which topics to discuss in conversation, such that more calibrated expectations encourage deeper conversation (Experiments 7a-7b). Misunderstanding others can encourage overly shallow interactions. (PsycInfo Database Record (c) 2022 APA, all rights reserved).