The present study is focused on seasonal variation in groundwater quality, hydrochemistry, and associated human health risk in the Shivganga river basin, Western Maharashtra, India, to promote sustainable development of groundwater resources of this semi-arid region. The qualitative geochemical analysis, contamination levels, and human health risk assessment (HHRA) of groundwater are integral steps in groundwater management in the Deccan Plateau basalt flow region of India. Representative groundwater samples (n = 68) collected from the Shivganga River basin area of Pune district, Maharashtra, during pre-monsoon (PRM) and post-monsoon (POM) seasons in 2015 were analyzed for major cations and anions. According to the World Health Organization (WHO, 2017) drinking standards, EC, total dissolved solids, hardness, bicarbonate, calcium, and magnesium surpassed the desirable limit. Boron and fluoride content exceeded the prescribed desirable limit of the WHO. The pollution and drinking suitability were assessed by computing pollution index of groundwater (PIG), groundwater quality index (GWQI), and HHRA particularly for boron and fluoride toxicity. PIG values inferred that about 6% of groundwater samples has moderate, 24% has low, and 70% has insignificant pollution in the PRM season, while only 1 sample (3%) showed high pollution, 6% showed low, and 91% showed insignificant pollution in the POM season. GWQI classification demonstrated that 27% and 15% samples are within the poor category, and only 15% and 18% of the samples fall into excellent water category in the PRM and the POM seasons, respectively. Total hazard index (THI) revealed that 88% of children, 59% of adults, and about 38% of infants are exposed to non-carcinogenic risk, as THI values (>1) were noted for the PRM season, while 62% of children, 47% of adults, and 24% of infants are vulnerable to non-carcinogenic health hazard during the POM period.