Results of chlorophyll fluorescence parameters (ChlF) informativity measurement and comparison of 10-d-old spring wheat seedlings under laboratory conditions under separate and combined stressors action are presented. It was found that separate and combined action of chloride salinity (1,3%), infection with cereal root rot pathogen Bipolaris sorokiniana Shoem. (5000 conidia per grain) suppressed light and dark reactions of photosynthesis. The effective quantum yield Y(II), photochemical quenching qP and electron transport ETR decreased significantly in both cultivars, most significantly in the co-activated version (up to 62,7%). The maximum photochemical quantum yield of FS II Fv / Fm was less informative, no significant changes in the parameter were found. Inhibition of light-dependent reactions was accompanied by a significant increase in the values of the parameters of non-photochemical quenching ChlF - coefficient qN and quantum yield of regulated non-photochemical quenching ChlF Y (NPQ) from 24.1 to 72.1% in both varieties, most pronounced in the variety Sibirskaya 12. The parameter Y(NO), the quantum yield of unregulated non-photochemical quenching of ChlF, changed insignificantly relative to the control in both varieties. The positive effect of seed pre-heating (43 °C) on the functional activity of photosynthetic apparatus of seedlings - the reliable (p ≤ 0,05) increase of the parameter Y(II), qP, ETR (by 18,0-59,0%) and decrease of the parameter Y(NPQ), Y(NO) and qN (by 18,8-35,1%) at further infection and chloride salinization in both sorts, mainly in the variety Omskaya 18 was revealed. The informativeness of the parameters ChlF for assessment of varieties stress tolerance was established. Significant intervariety differences (from 1.2-6.2 times) were revealed for almost all parameters (except for Fv / Fm, Y(NO), Fv) for all variants of experiment. The varietal specificity was established - the least changes in ChlF parameters relative to the control were in the stable variety Omskaya 18 in all variants of the experiment. The proposed approach will make it possible to develop a noninvasive method for early diagnosis of stress tolerance (phenotyping) of new wheat genotypes to biotic and abiotic stressors.