Curcumin Nanoparticles Research Articles

Nano apatite growth on demineralized bone matrix capped with curcumin and silver nanoparticles: Dental implant mechanical stability and optimal cell growth analysis

ObjectivesThe prevention of implant-associated infections is becoming increasingly clinically important in the field of dentistry. Extensive investigations into the development of innovative antibacterial materials that interact effectively to reinforce their functionality are currently being conducted in the biomedical sector. In the present study, a novel dental nano putty (D-nP) has been developed using demineralized bone matrix (DBM), calcium sulfate hemihydrate (CSH), curcumin nanoparticles (CU-NPs), and silver nanoparticles (AgNPs). MethodsThe produced D-nP was evaluated using physicochemical, mechanical, and in vitro analyses. Surface characterization, particularly the analysis of calcium and phosphorus content, was performed before and after immersion in the simulated body fluid (SBF). In addition, the impact of surface treatment on biological activity was studied. ResultsThe results showed that the mechanical properties of the D-nP were outstanding and its performance is promising. D-nP exhibited excellent antibacterial activity against Actinomyces naeslundii (5.22 ± 0.07 mm) and Streptococcus oralis (5.41 ± 0.1 mm). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was conducted using MG-63 osteoblast cells, which exhibited 95 % viability in D-nP. ConclusionsBased on these characterization results, the D-nP developed in this study exhibited excellent performance for tooth tissue in bone repair.

Effect of Adding Different Levels of Silver -Curcumin Nanoparticles on Some Productive Traits, Blood Parameters and Antioxidant Status of Broiler Chickens

The objective of the current study was to investigate the impact of adding different levels of silver-curcumin nanoparticles on some productive traits, blood parameters and antioxidant status of broiler chickens. A total of 144 broiler chicks (Ross308),one day old, with an average weight of 40 grams, were used in this study. The chicks were randomly distributed into four experimental treatments (each with 36 chicks), with three replicates for each treatment (12 chicks per replicate), using a completely randomized design for a period of 35 days. The first treatment was a control without addition, while the second, third, and fourth treatments, each added 1, 1.5, and 2 mg of silver-curcumin nanoparticles per liter of drinking water, respectively. The results indicated that the best average body weight, overall weight gain, and feed conversion efficiency were in the fourth treatment. The results indicated a significant increase (p≤0.05) in some blood parameters such as the packed cells volume , red blood cells, and hemoglobin concentration in the third and fourth treatments compared to the other experimental treatments. The results indicated a significant increase (p≤0.05) in the third and fourth treatments for the effectiveness of the GPX enzyme compared to the control and the second treatments. In terms of the CAT enzyme's efficiency, the results showed an improvement all treatments when compared to the control treatment. While the results did not indicate a significant difference in the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), the activity of the enzyme superoxide dismutase (SOD), and the concentration of malondialdehyde (MDA) among the study treatments. It can be concluded that adding 2 mg/L of silver- curcumin nanoparticles has improved the growth of broilers and their feed conversion efficiency, as well as improving blood parameters and enhancing antioxidant enzymes such as glutathione peroxidase and catalase.

Macrophage membrane-coated self-assembled curcumin nanoparticle missile for the treatment of colorectal cancer

Curcumin is a natural compound with various anti-cancer properties, but its poor solubility and low bioavailability limit its clinical application. In this study, we developed a novel nanoparticle system based on curcumin self-assembly, polydopamine modification and macrophage membrane coating (Cur-PDA@CM) to overcome these challenges. The Cur-PDA@CM nanoparticles exhibited high drug loading, enhanced stability, excellent photothermal effects and powerful anti-tumor efficacy. Moreover, the macrophage membrane endows the nanoparticles with active targeting ability to tumor tissues. We evaluated the anti-tumor efficacy of Cur-PDA@CM in vitro and in vivo using a murine colon carcinoma model. The results showed that Cur-PDA@CM could effectively inhibit tumor growth and induce apoptosis by releasing curcumin, generating reactive oxygen species and polarizing tumor-associated macrophages towards the M1 phenotype. The combination of Cur-PDA@CM with near-infrared irradiation further enhanced the therapeutic outcomes by facilitating drug release and photothermal therapy. The Cur-PDA@CM system represents a promising platform for delivering natural products with poor solubility and harnessing their full anti-cancer potential.

Evaluation of the therapeutic efficacy of curcumin nanoparticles in the intestinal and muscular phases of murine trichinosis

Evaluation of the therapeutic efficacy of curcumin nanoparticles in the intestinal and muscular phases of murine trichinosis

Physico-chemical properties of curcumin nanoparticles and its efficacy against Ehrlich ascites carcinoma

Curcumin is a bioactive component with anticancer characteristics; nevertheless, it has poor solubility and fast metabolism, resulting in low bioavailability and so restricting its application. Curcumin loaded in nano emulsions (Cur-NE) was developed to improve water solubility and eliminate all the limitations of curcumin. Size distribution, zeta potential, transmission electron microscopy (TEM) measurements, UV–Visible spectra, IR spectra and thermogravimetric analysis (TGA), were used to characterize the prepared Cur-NE. Cancer therapeutic efficacy was assessed by oxidative stress (superoxide dismutase (SOD), Glutathione–S–Transferase (GST), malondialdehyde (MDA) and nitric oxide (NO), DNA damage, apoptotic proteins (caspase-3 and 9), besides investigating tumor histology and monitoring tumor growth. Additionally, the cytotoxicity and genotoxicity of the liver, kidney, heart, and spleen tissues were examined to gauge the adverse effects of the treatment method’s toxicity. The results showed that Cur-NE is more effective than free curcumin at slowing the growth of Ehrlich tumors while significantly increasing the levels of apoptotic proteins. On the other hand, Cur-NE-treated mice showed some damage in other organs when compared to mice treated with free curcumin. Cur-NE has a higher efficacy in treating Ehrlich tumor.

Investigating the Selective Toxicity of Iron-Loaded Curcumin Nanoparticles on Fibroblast Cells Isolated from the Skin of an Animal Model of Diabetic Foot Ulcer

Investigating the Selective Toxicity of Iron-Loaded Curcumin Nanoparticles on Fibroblast Cells Isolated from the Skin of an Animal Model of Diabetic Foot Ulcer

Anticancer potential of blue-green algae extract (Spirulina plantesis) and Curcumin nanoparticles on Ehrlich ascites carcinoma–bearing mice

Anticancer potential of blue-green algae extract (Spirulina plantesis) and Curcumin nanoparticles on Ehrlich ascites carcinoma–bearing mice

Chitosan tamarind-based nanoparticles as a promising approach for topical application of curcumin intended for burn healing: in vitro and in vivo study

One of the most prevalent worldwide problems that affect all ages and genders is skin burn. The goal of our study was to assess the ability of curcumin nanoparticles to cure a rat burn model. Three formulations were selected after several tests were performed including investigation of encapsulation efficiency, particle size and zeta potential measurements. In vitro release was achieved on the three selected formulations. The effectiveness of the chosen formulation for healing was evaluated. The induced burn wound was smeared, starting just after excision, once daily with curcumin nanoparticles for 18 days. Our findings revealed that curcumin nanoparticles improved the burn healing potential by augmenting the skin regeneration indices as evidenced by enhancing the new production of hyaluronic acid and collagen type I. Additionally, curcumin nanoparticles could increase levels of vascular endothelial growth factor and alpha smooth muscle activity while drastically reducing the skin’s tumour necrosis factor content, revealing a significant potential for burn healing process that is also reflected in the histopathological and immunohistochemical studies. Finally, our results demonstrated that curcumin nanoparticles revealed a significant potential for burn healing than curcumin alone due to its potent antimicrobial, antioxidant and anti-inflammatory properties.

Evaluating the Antifungal Activity of Nano-curcumin against Candida Species Isolated from Otomycosis Patients

Background & Objective: Candida species are one of the most common causes of otomycosis. Antifungal drugs commonly used in treatment often have a variety of side effects, including toxicity, cross-reactivity, and drug resistance. In recent years, along with the advances in nanotechnology and the motivation to find new antifungal drugs, there has been a growing interest in the use of nanoparticles such as nano-curcumin in the treatment of fungal infections. The aim of this study was to evaluate the antifungal sensitivity of nano-curcumin on Candida species isolated from otomycosis.
 Materials & Methods: In this experimental study, 100 isolated Candida samples from patients with otomycosis were included. Synthesis of nano-curcumin using chitosan (CS) nanoparticles was performed by ionic gelation method. Antifungal susceptibility testing was performed using broth microdilution method according to CLSI-M27-S4 guidelines on all Candida isolates to miconazole, clotrimazole and nanocurcumin. Data were analyzed in SPSS 27 software with independent t-test and chi-squared tests.
 Results: Curcumin nanoparticles showed antifungal activity against all Candida species. However, the mean MIC of miconazole and clotrimazole for different Candida species was significantly lower than curcumin nanoparticles, indicating the lower antifungal effect of nano-curcumin than these two antifungals. Also, the mean MIC of nano-curcumin was not significantly different among Candida species (P < 0.05), whereas it was significantly different for miconazole and clotrimazole (P < 0.05).
 Conclusion: The results of the present study showed that nano-curcumin had a lower antifungal effect than miconazole and clotrimazole, but due to nano-curcumin's safety, it can be used as a potential antifungal drug for the treatment of otomycosis after further investigation.

(NaPO3)6-modulated reassembly of encapsulated curcumin nanoparticles from yolk granules: Preparation, characterization, and bioavailability

Our previous research has shown that yolk granules can act as a unitary carrier of lipophilic substances. In this study, the Ca2+ content was adjusted using (NaPO3)6 to control the reassembly of yolk granules for curcumin encapsulation to generate yolk granule-curcumin nanoparticles (Gra-Cur NPs). (NaPO3)6 reduced the particle hydrodynamic diameter (Dh). Dissociated granules had the same protein profile as untreated granules, indicating that no new protein subunits were produced. Hydrophobic interaction was the main force of curcumin binding to yolk granules, and curcumin is amorphous after encapsulated. Gra-Cur NPs had excellent thermal and storage stability. Flow cytometry revealed that the rate of HT-29 cell apoptosis and curcumin absorption of encapsulated curcumin was higher than that of curcumin dissolved in DMSO, with smaller Dh. The findings of this study will allow for the development and implementation of bioactive compounds as novel carriers.

Enhance potato resistance to Potato virus YNTN using curcumin nanoparticles Inhibitory Effects of Curcumin nanoparticles on Potato Plants Infected by Potato Virus Y

Enhance potato resistance to Potato virus YNTN using curcumin nanoparticles Inhibitory Effects of Curcumin nanoparticles on Potato Plants Infected by Potato Virus Y

Encapsulation of Sericin-Decorated Efficient Agents in Silk Hydrogels for Wound Dressings.

Excessive oxidative stress, bacterial infections, and inflammation are the primary factors impeding the healing of skin wounds. Bioactive hydrogels are commonly employed in the treatment of skin injuries. However, the limited solubility of many drugs and active agents in water significantly hampers their effectiveness in hydrogel dressings. In this research, prior to incorporation into the silk fibroin (SF) hydrogel matrix, two active agents curcumin and silver nanoparticles (Ag NPs) were decorated by silk sericin to improve their dispersibility and stability in water. The resultant SF/Ag/C hydrogels combined the biological safety and nontoxicity of SF, the antioxidant and anti-inflammatory efficacy of curcumin, and the antibacterial effect of Ag NPs. These properties effectively enhanced wound repair by reducing bacterial infections, mitigating oxidative stress, suppressing the expression of pro-inflammatory factors, and promoting angiogenesis. This study presented a straightforward approach for constructing bioactive hydrogels for the promotion of the wound healing process.

Curcumin nanoparticles as a natural antioxidant and antimicrobial preservative against foodborne pathogens in processed chicken fingers

IntroductionCurcumin has highly antimicrobial and antioxidant activities but has poor bioavailability and low solubility in water. The current study aimed to assess curcumin nanoparticles (Curcumin-NPs) antibacterial and antioxidant efficacy against some pathogens in chicken fingers at 4 °C/27 days.MethodsCurcumin-NPs with particle sizes of 80 ± 2 nm were synthesized using a planetary ball-mill and chitosan-gelatin nanoparticle (CS-G-NPs) solution and then placed into chicken fingers at three concentrations, (2, 5, and 10 μg g−1). The physicochemical properties, antioxidant activity, and antibacterial capacity were evaluated.Results and discussionCurcumin-NPs showed high phenolic content (67.48 mg GAE g−1) and antioxidant activity (22.47 μg ml−1) at 10 μg g−1 compared to other concentrations and curcumin bulk. Curcumin-NPs showed noticeably greater antibacterial ability (in vitro) against S. aureus (18 mm), E. coli (15 mm), and B. cereus (13 mm). In challenge studies, Curcumin-NPs effectively inhibited the three inoculated pathogens ~3–4 log CFU g−1; in vivo; in chicken fingers kept for up to 27 days, compared to the control. In curcumin-NPs chicken samples, the contents of thiobarbituric acid reactive substances (TBARS) and total volatile base nitrogen (TVB-N) compared to the control were substantially lower (27 days). TEM analysis provided an estimate of the antibacterial mechanism of Curcumin-NPs. The findings demonstrated that Curcumin-NPs at 10 μg g−1 were more successful in reducing microbial load in chicken fingers as well as improving quality parameters, enhancing shelf life, and reducing lipid oxidation in poultry meat products.

Effectiveness of curcumin nanoparticles in rat liver fibrosis caused by thioacetamide.

Although curcumin possesses anti-inflammatory, antioxidant, and cytoprotective qualities, its low absorption limits its medicinal uses. Before examining how curcumin influenced rats' liver fibrosis when thioacetamide (TAA) was produced, the current study employed nanoparticles (NPs) to improve curcumin bioavailability. Sixty mature rats were separated into six groups (Group 1, control; Group 2, curcumin; Group 3, curcumin nanoparticles; Group 4, TAA; Group 5, TAA + curcumin; Group 6, TAA + curcumin NPs). TAA administration caused considerable increases in serum liver enzymes associated with a remarkable depletion in the levels of albumin and total protein relative to the control. In addition, a significant elevation in malonaldehyde (MDA) level with a significant depletion in the antioxidant enzymes activity was detected. Also, TAA had a significant effect on the inflammation markers represented by the elevation in tumor necrosis factor (TNFα) and DNA damage. Administration of curcumin or curcumin NPs in TAA-intoxicated rats significantly (p < .001, p < .0001) alleviates liver injury by correcting antioxidant status, inflammatory markers, and oxidative stress. The results of comparing TAA-intoxicated rats treated with curcumin NPs to TAA-intoxicated rats treated with bulk curcumin revealed that the ameliorative effect of nanocurcumin was stronger. These observations concluded that nanoparticle formulation can increase curcumin bioavailability and solubility, enhancing its antioxidant and anti-inflammatory efficiency, resulting in greater potential against thioacetamide-induced hepatotoxicity in rats.

Site-Specifically Launched Microneedles for the Combined Treatment of Psoriasis-Diabetic Comorbidity.

Psoriasis and diabetes are both common comorbidities for each other, where inflammation and insulin resistance act in a vicious cycle, driving the progression of disease through the activation of the NF-κB signaling pathway. Therefore, disrupting the linkage between inflammation and insulin resistance by inhibiting the NF-κB pathway presents a promising therapeutic strategy for addressing psoriasis-diabetic comorbidity. Herein, an open-loop therapy was developed by integrating microneedle-mediated short- and long-range missiles to target psoriasis and diabetes, respectively. The short-range missile (curcumin nanoparticle) could be stationed in the psoriatic skin for topical and prolonged antipsoriasis therapy, while the long-range missile (metformin) is capable of penetrating transdermal barriers to induce a systemic hypoglycemic effect. More attractively, the short- and long-range missiles could join hands to inhibit the NF-κB signaling pathway and diminish inflammation, effectively disrupting the crosstalk between inflammation and insulin resistance. Pharmacodynamic studies showed that this microneedle-mediated combination, possessing dual anti-inflammatory and antihyperglycemic properties, proves to be highly efficacious in alleviating typical symptoms and inflammatory response in both nondiabetic and diabetic mice with imiquimod (IMQ)-induced psoriasis models. Hence, the microneedle-mediated open-loop therapy shows great potential in the management of psoriasis-diabetes comorbidity.

The Effect of Curcumin Nanoparticles on Paracetamol-Induced Liver Injury in Male Wistar Rats.

Curcumin is a naturally occurring compound that has antioxidant properties, acts as a hepatoprotective, and lowers lipid peroxidation. However, curcumin's low solubility and bioavailability are its primary drawbacks and prevent its use as a therapeutic agent. In this study, curcumin nanoparticles will be created using the ultrasonic-assisted extraction method, and their effectiveness against paracetamol-induced changes in ALT, AST, SOD, MDA, and TNF-α will be compared to that of pure curcumin. This study aimed to determine the hepatoprotective effect of curcumin nanoparticles in paracetamol-induced rats as a model for liver injury. Thirty-six male Wistar rats, aged 6 to 8 weeks, with a minimum weight of 120 grams, were used in an experimental laboratory investigation with a post-test-only group design. Rats in each group received 100 mg/kgBW pure curcumin, 100 mg/kgBW curcumin nanoparticles, and 50 mg/kgBW curcumin nanoparticles for 7 days before paracetamol induction. On day 8, 300 mg/kgBW of paracetamol was intraperitoneally injected to cause liver damage. One of the groups received NAC as an antidote 10 hours after paracetamol induction. Detection of ALT and AST using a Chemistry Analyzer. ELISA approach for the detection of SOD, MDA, and TNF-α. The Roenigk score was calculated by two examiners after the liver histopathology preparations were stained using the Hematoxylin-Eosin method. Post hoc analyses were performed after the One Way Annova and Kruskal Wallis tests to examine the data. According to PSA results, the smallest formula that formed curcumin nanoparticles (10.2 nm) was 8 g of curcumin formula mixed with a mixture of Tween 20 4.5 ml, Kolliphor EL 1.5 ml, Propylene Glycol 1.5 ml, and Capryol 90 1 ml for 21 minutes using an ultrasonic process. MDA and TNF-α levels, as well as the liver's histological Roenigk score, were significantly lower in the 100 mg/kgBB pure curcumin group (C100) when compared to the model group (model). The levels of AST, MDA, TNF-α, and the liver histopathology score were significantly lower in the 100 mg/kgBB (NC100) and 50 mg/kgBB (NC50) curcumin nanoparticle groups compared to the model group (model) and pure curcumin group (C100) (p< 0.05). Curcumin nanoparticles showed better hepatoprotective ability than pure curcumin.

Curcumin nanoparticles and the therapeutic potential of curcumin for musculoskeletal disorders.

Musculoskeletal disorders (MSD) are a collection of degenerative conditions impacting the body's bones, joints, muscles, tendons, ligaments, and nerves. MSDs affect approximately 1.71 billion individuals worldwide and are a significant cause of disability. Curcumin is a polyphenolic compound with anti-inflammatory, antioxidant, and antitumor properties. In this review, we will discuss the research progress of structural analogs, derivatives, and nanomaterials that can improve the bioavailability of this natural drug. Curcumin may potentially retard the progression of osteoporosis, osteoarthritis, and rheumatoid arthritis. These effects may be related to curcumin's targeting of multiple signalling pathways.

The impact of curcumin nanoparticles (CurNPs) on growth performance, antioxidant indices, blood biochemistry, gut morphology and cecal microbial profile of broiler chickens

ABSTRACT A total 375 one-day-old male broiler chickens were allotted into five groups including: 0, 25, 50, 100 and 200 mg curcumin nanoparticles) CurNPs). Final body weight increased and feed conversion ratio improved by CurNPs supplementation. Antioxidant enzymes activities were increased and malondialdehyde was reduced by CurNPs. The white blood cells (except heterophil) increased and red blood cells and hematocrit decreased by CurNPs supplementation. The serum enzyme activity, cholesterol and triglyceride reduced and protein increased by CurNPs supplementation. Also, The CurNPs, increased relative weight of spleen and immune indices concentrations in serum. Moreover, The CurNPs addition improved gut morphological traits in the Jejunum. CurNPs supplementation. Moreover, CurNPs supplementation tended to lower cecal E. coli, coliform, aerobic, anaerobic bacteria, and total yeast and molds count, in concomitance to greater Lactic acid bacteria population.

A Smart Nanovector of Cationic Starch Modified Curcumin with Excellent Targeting and Sonodynamic Properties for Effective Therapy of MRSA‐Induced Osteomyelitis

AbstractDrugs treatment including antibiotics or herbal medicines cannot cure osteomyelitis effectively accompanied by drug‐resistance bacteria and even amputation after long‐term debridement. Herein, a materioherbological strategy is utilized to construct a smart nanovector of cationic starch (CS) modified curcumin nanoparticles (CS@Cur NPs) for treating methicillin‐resistant Staphylococcus aureus (MRSA) induced osteomyelitis. On the one hand, CS modification can reduce the π–π accumulation of curcumin to endow the nanocomposite with excellent water solubility. On the other hand, the hydroxyl group in the curcumin is hydrogen‐bonded to the carbonyl group in the monomer (methyl methacrylate, MMA) of CS, resulting in a decrease in both the bandgap (between the highest and lowest occupied molecular orbitals, labeled as ΔE) and the S1–T1 energy gap (ΔES‐T) while the spin–orbital coupling (SOC) values increase, which benefits the formation of triplet‐state molecules under ultrasonic irradiation, significantly improving the yields of reactive oxygen species (ROS) capacity. The CS@Cur NPs nanocomposite constructed in this paper produced a large number of ROS synergic targeting effects under continuous 15 min ultrasonic irradiation, the clearance rate of MRSA infection induced osteomyelitis in rats can be as high as 99.93%. This study provides a new therapeutic strategy for the treatment of osteomyelitis induced by pathogenic bacteria.

Comparable bio-evaluation of curcumin and chitosan-encapsulated curcumin nanoparticles against the reprotoxic potential of fenpropathrin pyrethroid in rats: Genomic and morphometric prospectives.

This study delves into the intricate exploration of potential toxic effects resulting from subchronic exposure to fenpropathrin (FNP) on the reproductive system of male SD rats. Adding to the novelty, our study undertakes a pioneering comparison of the effects of curcumin (CUR) and curcumin-encapsulated chitosan nanoparticles (CS.CUR.NPs) on these toxic effects. The study involved a cohort of sixty male SD rats (six groups): vehicle control, CUR, Cs.CUR.NPs, FNP, and two combination groups (FNP with CUR or Cs.CUR.NPs). The synthesized Cs.CUR.NPs nanoparticles underwent meticulous characterization using Fourier Infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM). The findings revealed that FNP caused oxidative stress, sperm abnormalities, reduced motility and sperm count FNP decreased serum LH, FSH, 17-β estradiol, and testosterone levels. FNP downregulated the mRNA expression of the spermatogenesis and steroidogenesis-related genes, While, downregulated hypothalamic KISS-1 and KISS-1r expression. Histopathological alterations were assessed and scored. Surprisingly, the treatment with CUR and Cs.CUR.NPs exhibited remarkable restorative effects on semen quality, sex hormone levels, antioxidant capacity, and mRNA expression of the targeted genes. Notably, Cs.CUR.NPs displayed superior properties when compared to CUR. Nevertheless, further research is imperative to evaluate their efficacy across various bodily tissues.