Cu In Seawater Research Articles

Copper as a Complex Indicator of the Status of the Marine Environment Concerning Climate Change

Studies covering key elements of the marine ecosystem based on current and long-term data have made it possible to assess both the current situation in terms of copper concentrations in commercially used fish and benthic plants and in surface bottom sediments, as well as enabled the analysis of the temporal variability of copper levels in relation to changes in its inflow to the southern Baltic Sea. By applying the threshold values, determining the boundary between good and not good status of the marine environment, set in this study, it was found that good environmental status has been achieved in the case of Cu in seawater and plants and has not been achieved in the case of sediments and fish for consumption. The study showed that climate change, the main feature of which is an increase in seawater temperature, significantly impacts the distribution and levels of copper in individual elements of the marine environment. It influences the vegetative season length and bioaccumulation efficiency and is of key importance for copper toxicity.

An antifouling gel-protected iridium needle sensor: Long-term, on-site monitoring of copper in seawater

Copper (Cu), a natural micronutrient with ecotoxicological significance, is involved in the carbon and nitrogen cycles occurring in marine ecosystems. Here, we developed a novel, antifouling gel-protected iridium (Ir) needle electrode modified with gold nanoparticles (G-IrNS) for long-term continuous and steady Cu monitoring. The gel formed an efficient membrane that effectively prevented the fouling of the sensing surface and displayed anti-convective properties, ensuring that mass transport toward the sensor surface was wholly controlled via diffusion. The repeatability, reproducibility, and stability of G-IrNS showed that it was suitable for long-term and on-site monitoring of Cu in seawater. Cu concentrations were successfully measured via fixed-point continuous monitoring for >2 weeks and onboard continuous monitoring in Bohai Sea using one sensor. Moreover, the relationship between Cu concentrations measured on-site via G-IrNS and its dissolved concentration in Bohai Sea was evaluated. G-IrNS can be applied to other metal ions as well, especially for long-term automatic on-site monitoring, thereby providing a basis for further research.

Wide-range detection of Cu-binding organic ligands in seawater using reverse titration

The toxicity and bioavailability of Cu in seawater were estimated using its chemical speciation. In this study, the concentrations and conditional stability constants (K'CuL) of Cu complexes with Cu-binding organic ligands (L) in seawater were determined using the reverse titration–competitive ligand exchange–adsorptive cathodic stripping voltammetry method. The concentrations of strong and weak ligands (L1 and L2, respectively) were determined using reverse titration via the addition of Cu to seawater samples to achieve ligand saturation. Our results revealed that the reverse titration method can be successfully used for the detection of high concentrations of L2. Using the reverse titration method, we detected L2 concentrations that exceeded 80 and 120 nM in seawater samples from the subtropical western North Pacific Ocean and Otsuchi Bay Japan, respectively. The K′CuL1 and K′CuL2 values obtained using the reverse titration method were comparable with those obtained using the forward titration method. Therefore, the reverse titration method can be used to determine the chemical speciation of Cu in the ocean and coastal regions as supplement to the more conventional forward titration method.

Isotopic disequilibrium of Cu in marine ferromanganese crusts: Evidence from ab initio predictions of Cu isotope fractionation on sorption to birnessite

In the oceans, Cu is strongly scavenged by ferromanganese (Fe-Mn) crusts. The isotopic fractionation of Cu between seawater and crusts provides insight into the mechanisms of trace metal cycling in the oceans. Dissolved Cu in seawater is isotopically heavy (+0.66±0.19‰) relative to Cu in crusts (+0.31±0.24‰). The primary mineral phase sorbing divalent trace metals in Fe-Mn crusts is birnessite. Recent laboratory measurements show that isotopically light Cu is preferentially sorbed on birnessite, with a fractionation factor of 0.45±0.18‰. Here, we use first-principles (quantum mechanical) calculations to predict the isotopic fractionation between aqueous Cu2+ complexes and Cu as a surface complex on birnessite. We find that isotopic fractionation between the Cu(H2O)5+ complex and sorbed Cu should be 0.49‰ (at 25°C), in close agreement with experiments, confirming that these experimental results reflects equilibrium fractionation. We then predict the isotopic fractionation between dissolved inorganic Cu in seawater and birnessite given the thermodynamic speciation of dissolved Cu at pH 8. We find dissolved inorganic Cu should be 0.94‰ (at 5°C) heavier than Cu sorbed to birnessite. This value is substantially greater than the observed fractionation between seawater and Fe-Mn crusts (Δsw-fmc≈+0.35‰). Moreover, it is well established that dissolved Cu in seawater is strongly complexed by organic ligands. Based on model Cu complexes and published experimental data, we estimate that fractionation of Cu by organic ligands should increase the equilibrium fractionation between seawater and Fe-Mn crusts by 0.2 to 1.5‰ to yield Δsw-fmc = +1.1 to 2.4‰. We conclude that Cu in marine Fe-Mn crusts in not in isotopic equilibrium with dissolved Cu in seawater, and consider the possible explanations of this surprising finding.

Baseline study on the levels of heavy metals in seawater and macroalgae near an abandoned mine in Manicani, Guiuan, Eastern Samar, Philippines.

Lateritic nickel ore stockpiles are still present in Manicani Island after mining activities were suspended in 2001. This study aimed to assess the water quality in the island by measuring the levels of Pb, Ni and Cu in seawater and Sargassum polycystum samples collected quarterly in 2018. The levels of the three heavy metals in seawater samples were 0.388 ± 0.058-1.508 ± 0.120 mg/L Pb, 0.457 ± 0.003-0.531 ± 0.005 mg/L Cu, and 0.122 ± 0.008-0.628 ± 0.281 mg/L Ni. All values are above the permissible limits recommended by DENR and USEPA. Ni (13.630 ± 7.341-160.120 ± 3.375 mg/kg) had the highest concentration in S. polycystum, significantly higher than Pb (<LD-21.579 ± 0.730 mg/kg) and Cu (2.825 ± 0.110-6.302 ± 0.864 mg/kg). Ni levels in S. polycystum near the mining runoff were significantly higher than in samples from other stations. The results of this study show the contribution of the abandoned mine to the heavy metal pollution of the marine waters in Manicani Island.

The heavy metals lead (Pb), chromium (Cr), copper (Cu) and cadmium (Cd) contents in the white shell Meretrix meretrix Linnaeus, 1758

The research about the contain of heavy metals lead (Pb), chromium (Cr), copper (Cu) and cadmium (Cd) in the soft body of the white shells Meretrix meretrix Linnaeus, 1758 from local market Makassar has been conducted. This research was aimed to determine heavy metals Pb, Cr, Cu and Cd content in the white shells M. meretrix Linnaeus from local markets of Makassar. Heavy metal contain in white shells, sea water and sediment were analysed using Atomic Absorption Spectrophotometer (AAS). The result of the analysis shows that the content of Pb in M. meretrix L. from the central market and the Tanjung market are 8,322 and 5,627 ppm. Cr content in shell from Sentral market and Tanjung market was 0.102 and 0.052 ppm. Moreover, copper (Cu) and cadmium (Cd) content in white shells was not detected. The sea water from Metro Tanjung Bunga contains Cd 0.33 ppm and Cu was not detected. While the heavy metal content of Cd and Cu in sea water from Pangkep was not detected. The sediment from Metro Tanjung Bunga, contain Cd was 1.38 ppm and Cu was 1.84 ppm. While the heavy metal contain of Cd in sediment from Pangkep was not detected and Cu was 15.96 ppm. The content of metal Pb and Cr in M. meretrix L. exceeds the environmental quality standard.

Spatial distribution and potential ecological risk assessment of heavy metals in the North-West Coast of Kundur Island, Kepulauan Riau Province, Indonesia

The objective of the present study was to analyze spatial distribution potential ecological risk of heavy metals in North-West Coast of Kundur Island. Sampels of seawater and sediment were taken from five stations and were analysed by using AAS Perkin Elmer 3110 in Marine Chemistry Laboratory Faculty of Fisheries and Marine Science, Universitas Riau. The arrangement of the metals studied from higher to lower mean concentrations are: Fe>Ni>Zn>Mn>Cr>Sn>Pb>Cu in seawater and Fe>Mn>Zn>Ni>Cr>Pb>Sn>Cu in sediments, respectively. Distribution of heavy metal concentrations in seawater and sediment were found to have almost similar trends where higher concentrations were found in stations closed to the mining activities. Concentration of all metals in seawater were possitively correlated with that in sediment, except for Mn. Contamination factor, geoaccumulation index and enrichment factor demonstrated that most of the sediment samples were moderately to heavily contaminated by Cr, Ni and Zn but not yet for other metals. However, PERI value indicated that the studied area is still has low ecological risk from the mining activity in the surrounding area.

Determination of the copper isotope composition of seawater revisited: A case study from the Mediterranean Sea

A new technique for the determination of dissolved copper isotope composition (δ65Cu) of seawater was applied to examine copper sources and internal cycling in the Mediterranean Sea along the GEOTRACES GA04N transect. A succession of chelating resin with nitrilotriacetic acid functional groups and strong base anion exchange resin, together with optimization of the multi-collector inductively coupled plasma mass spectrometry set-up allowed to isolate copper from seawater matrix and to measure the 65Cu/63Cu ratios in seawater with an external precision of 0.06‰ (2 s.d.). This method was first applied for inter-comparison measurements to surface and deep waters sampled at the Bermuda Atlantic Time-Series station (BATS) in the North Atlantic Ocean during the GEOTRACES Intercalibration Cruise 1. Disparities in δ65Cu reported here and in the literature over these samples showed a need to investigate whether a new inter-comparison sample should be decided upon, or whether the use of UV-oxidation can also affect the measurement of δ65Cu. We also measured total dissolved Cu concentration ([dCu]T) and δ65Cu for 12 stations in the Mediterranean Sea. The δ65Cu distribution showed significant variations in the euphotic zone, at the Chl a maximum and at bottom depths. Copper isotope ratios ranged from +0.21‰ to +0.76‰ (±0.06‰, 2 s.d.), yielding an average of +0.51‰ (±0.20‰, 2 s.d.; n = 96) for the Mediterranean Sea. A strong zonation between natural aerosol deposition to the South and anthropogenic aerosol deposition to the North was seen in the sample set (Dulaquais et al. 2017; Gerringa et al. 2017; Rolison et al. 2015). Natural dust deposits seemed to draw δ65Cu toward lower values and deeper in the euphotic zone whereas the impact of anthropogenic aerosols seemed restricted to an increase in [dCu]T. At the Chl a maximum, δ65Cu showed significant increase which we attributed to scavenging on and/or uptake by phytoplankton. The isotope signature of Cu sources to the Mediterranean Sea was also investigated and we inferred a significant source of isotopically heavy Cu in the Gibraltar area, potentially originating from a release of Cu sulfide leached in the mining area of Southern Spain (i.e. Iberian Pyrite Belt) in rivers and transported to the Mediterranean Sea in surface by seasonal water mass circulation. Conversely, marine sediments appear to be a source of isotopically-light Cu to deep waters. This study provides new hints on the use of copper isotopes to study sources and sinks of Cu in seawater. This article is part of a special issue entitled: “Cycles of trace elements and isotopes in the ocean – GEOTRACES and beyond” - edited by Tim M. Conway, Tristan Horner, Yves Plancherel, and Aridane G. González.

Needle-shaped electrode for speciation analysis of copper in seawater

Here, a needle-shaped electrode based on commercial acupuncture needle was fabricated and functionalized with dendritic gold nanostructures (AuNDs) and ion-exchange polymer (Nafion) for voltammetric speciation analysis of copper (Cu) in seawater. To achieve the enhanced performance for voltammetric determination of Cu in seawater, the acupuncture needle electrode (ANE) was functionalized with AuNDs via electrochemical deposition and covered with Nafion. AuNDs facilitate the electrochemical responses of Cu due to their excellent catalytic and electroconductive properties, whereas Nafion can stabilize the AuNDs deposited on the surface of the needle tip and enhance the voltammetric responses through its specific cation-exchange ability with Cu2+. The so-designed AuNDs/Nafion/ANE showed excellent performance for the determination of Cu in complex matrix like seawater. Under the optimal conditions, the response of Cu at this functional needle-shaped electrode was linear in the concentration range from 0.05 to 1 nmol L−1 with an ultralow detection limit of 15.4 pmol L−1. Additionally, the functional needle-shaped electrode has been successfully used for the direct determination of different Cu species in seawater via anodic stripping voltammetry.

Organic complexation of copper in estuarine waters: An assessment of the multi-detection window approach

Abstract Speciation of dissolved Cu in seawater is mainly dominated by organic complexation . However, the analysis is complicated by the presence of many different classes of naturally-occurring organic ligands. In this study, we successfully determined the total dissolved Cu concentrations, Cu-complexing ligand concentrations and their conditional stability constants in Japanese estuarine waters using competitive ligand exchange adsorptive stripping voltammetry (CLE-AdCSV) coupled with a multi detection window approach. The Cu speciation parameters were calculated using ProMCC software. The use of the 5 μM SA detection window was sufficient and effective in detecting two ligand classes in the waters of Otsuchi Bay. The concentrations of the strong ligands (L 1) were between 1.95 and 4.21 nM, while the concentrations of the weak ligand (L2) were in the range of 6.3–33 nM. The conditional stability constants of the strong ligand were around 1015, while those of the weak ligands averaged around 1013. In all of the samples, the L1 concentrations exceeded the total dissolved Cu concentrations, and the two parameters were strongly correlated. Free Cu2+ concentrations were between 10−13 and 10−15 M, a range that is not toxic for most phytoplankton .

Study of copper (Cu) contents in blood cockles (Anadara sp.) at Surabaya coastal waters

Heavy metal pollution categorized as harmful effect on the environment. This study aims to determine the content and the rate of accumulation of heavy metals copper (Cu) in the mussel of cockles (Anadara sp.) This study uses observation method with descriptive analysis. The test results Cu in sea water was <0.0223 mg/L. The results have exceeded the water quality standard according to state environmental ministerial decree number 51 2004 on marine water quality standards for marine biota maximum limit of Cu in sea water is 0.008 mg/L. Copper concentration in the sediment of 6.16 mg/kg at the Wonokromo station and at the Dadapan station is 4.03 mg/kg. According to the US-EPA in 2004 on the instructions of sediment pollution classification 49.98 mg Cu/kg. The results of shells fur Cu on Wonokromo was 1.833 mg/kg and Dadapan was 1.352 mg/kg. According to the MOH Decree No.0375/B/SK/1989 about Quality Standard Biota Consumption of Metals to the maximum limit of the existing Cu concentrations in marine biota was 20 ppm or 20 mg / kg so that the content of Cu in shellfish feathers still below the limit of existing quality standards.

Analisis Tembaga (Cu) Dan Timbal (Pb) dalam Air Laut dan Sedimen di Perairan Pantai Loli Kecamatan Banawa Kabupaten Donggala

This study aimed to determine the concentration of heavy Cu and Pb in seawater and sediment in coastal waters Loli subdistrict Banawa, district Donggala. The method used was destruction and using Atomic Absorption Spectrophotometer (AAS) instrument.Samples of seawater and sediment were taken by two sampling points. Samples were destructed by using a solution of HNO3 and HClO4with ratio 4: 1. Levels were measured at a wavelength of 325.7 nm for Cu and 217 nm for Pb. The analysis result obtained that the levels of Pb in the seawater in the range of 0,531-0.671 mg/L, while for Cu in the range of 0.009-0.090 mg/L it can be concluded that the levels of Pb and Cu in seawater has exceeded the levels were of 0,008 mg/L. For sediment samples obtained that Pb levels were around 1,78-7,73 mg/kg while for Cu 15.86-68.88 mg/kg so Cu in sediment was up to 30 mg/kg while the levels of Pb did not exceed the tolerance limits of 33 mg/kg.

Effects of increasing temperature alone and combined with copper exposure on biochemical and physiological parameters in the zooxanthellate scleractinian coral Mussismilia harttii

Effects of increasing temperature alone and in combination with exposure to dissolved copper (Cu) were evaluated in the zooxanthellate scleractinian coral Mussismilia harttii using a marine mesocosm system. Endpoints analyzed included parameters involved in metabolism [maximum photosynthetic capacity of zooxanthellae (Fv/Fm), chlorophyll a and ATP concentrations], calcification [carbonic anhydrase (CA) and Ca2+-Mg2+-ATPase activity], and oxidative status [antioxidant capacity against peroxyl radicals (ACAP) and lipid peroxidation (LPO)]. Coral polyps were collected, acclimated and exposed to three increasing temperature conditions [25.0±0.1°C (control; average temperature of local seawater), 26.6±0.1°C and 27.3±0.1°C] using a marine mesocosm system. They were tested alone and in combination with four environmentally relevant concentrations of dissolved Cu in seawater [2.9±0.7 (control; average concentration in local seawater), 3.8±0.8, 5.4±0.9 and 8.6±0.3μg/L] for 4, 8 and 12days. Fv/Fm reduced over the experimental period with increasing temperature. Combination of increasing temperature with Cu exposure enhanced this effect. CA and Ca2+-Mg2+-ATPase activities increased up to 8days of exposure, but recovered back after 12days of experiment. Short-term exposure to increasing temperature or long-term exposure to the combination of stressors reduced LPO, suggesting the occurrence of a remodeling process in the lipid composition of biological membranes. ACAP, ATP and chlorophyll a were not significantly affected by the stressors. These findings indicate that increasing temperature combined with exposure to dissolved Cu increase susceptibility to bleaching and reduce growth in the zooxanthellate scleractinian coral M. harttii.

Determination of Ni, Co, and Cu in seawater by total external reflection X-ray fluorescence spectrometry

Total external reflection X-ray fluorescence spectrometry is a promising method for the highly sensitive determination of heavy metals in seawater, offering such advantages as the mobility of the used equipment and the low cost of analysis. Various methods of sample preparation to the analysis of seawater aimed at the elimination of interferences with the salt matrix were compared. It was shown that the method based on the preliminary extraction of metals as diehyldithiocarbamate complexes followed by back extraction is characterized by the highest efficiency. A procedure was developed for the determination of Ni, Cu, and Co in seawater with limits of detection at a level of 0.16–0.26 μg/L.

Automated preconcentration of Fe, Zn, Cu, Ni, Cd, Pb, Co, and Mn in seawater with analysis using high-resolution sector field inductively-coupled plasma mass spectrometry

A rapid, automated, high-throughput analytical method capable of simultaneous analysis of multiple elements at trace and ultratrace levels is required to investigate the biogeochemical cycle of trace metals in the ocean. Here we present an analytical approach which uses a commercially available automated preconcentration device (SeaFAST) with accurate volume loading and in-line pH buffering of the sample prior to loading onto a chelating resin (WAKO) and subsequent simultaneous analysis of iron (Fe), zinc (Zn), copper (Cu), nickel (Ni), cadmium (Cd), lead (Pb), cobalt (Co) and manganese (Mn) by high-resolution inductively-coupled plasma mass spectrometry (HR-ICP-MS). Quantification of sample concentration was undertaken using isotope dilution for Fe, Zn, Cu, Ni, Cd and Pb, and standard addition for Co and Mn. The chelating resin is shown to have a high affinity for all analyzed elements, with recoveries between 83 and 100% for all elements, except Mn (60%) and Ni (48%), and showed higher recoveries for Ni, Cd, Pb, Co and Mn in direct comparison to an alternative resin (NOBIAS Chelate-PA1). The reduced recoveries for Ni and Mn using the WAKO resin did not affect the quantification accuracy. A relatively constant retention efficiency on the resin over a broad pH range (pH 5–8) was observed for the trace metals, except for Mn. Mn quantification using standard addition required accurate sample pH adjustment with optimal recoveries at pH 7.5 ± 0.3. UV digestion was necessary to increase recovery of Co and Cu in seawater by 15.6% and 11.4%, respectively, and achieved full break-down of spiked Co-containing vitamin B12 complexes. Low blank levels and detection limits could be achieved (e.g., 0.029 nmol L−1 for Fe and 0.028 nmol L−1 for Zn) with the use of high purity reagents. Precision and accuracy were assessed using SAFe S, D1, and D2 reference seawaters, and results were in good agreement with available consensus values. The presented method is ideal for high throughput simultaneous analysis of trace elements in coastal and oceanic seawaters. We present a successful application of the analytical method to samples collected in June 2014 in the Northeast Atlantic Ocean.

Performance of Seawater Activated Battery as Alternative Energy Resources

Research on Freshwater and Seawater batteries, which using Al and Cu electrodes at variation of different electrolyte salinity, distance between electrodes and flowrate of electrolyte has been carried out. The galvanostatic discharge and Electrochemical Impedance Spectroscopy (EIS) of Al and Cu in seawater were investigated. The results showed the optimum condition with significant average potential discharge of 45,02 mV and current of 0,440 mA obtained from combination of 5% electrolyte salinity, 2 cm space between electrodes and 0,5 liter/hour electrolyte rate. EIS studies revealed that the Al and Cu interficial electrochemical process in seawater was determined by an activation controlled reaction

Performance of Seawater Activated Battery as Alternative Energy Resources

Research on Freshwater and Seawater batteries, which using Al and Cu electrodes at variation of different electrolyte salinity, distance between electrodes and flowrate of electrolyte has been carried out. The galvanostatic discharge and Electrochemical Impedance Spectroscopy (EIS) of Al and Cu in seawater were investigated. The results showed the optimum condition with significant average potential discharge of 45,02 mV and current of 0,440 mA obtained from combination of 5% electrolyte salinity, 2 cm space between electrodes and 0,5 liter/hour electrolyte rate. EIS studies revealed that the Al and Cu interficial electrochemical process in seawater was determined by an activation controlled reaction

Redox interactions of Fe and Cu in seawater

The interaction between the redox chemistry of Fe and Cu at nanomolar has been studied in UV-treated seawater. The oxidation of Fe(II) was studied as a function of concentrations of Cu(II) and Cu(I) from 0 to 200nM. The effect of added H2O2 (0–500nM), pH (6.0–8.5) and NaHCO3 (2–9mM) on the Fe(II) rate constants was studied at Cu(II) levels (0–200nM). To understand the competition between Fe and Cu, the reduction of Cu(II) to Cu(I) was also studied as a function of oxygen (air-saturated and anoxic seawater), Fe(II) (0–200nM) and H2O2 (0–300nM). The Fe(II) oxidation was accelerated by the presence of Cu(II) and Cu(I). This acceleration has been explained by the redox coupling between Fe and Cu, competition for different inorganic species (hydroxyl and carbonate groups studied independently) and by the formation of Fe–Cu particles (cupric or cuprous ferrite). Superoxide played a key role in the oxidation rate of Fe(II) in the presence of Cu(II). The presence of Fe(II) caused a greater reduction of Cu(II) to Cu(I). This is directly related to the levels of oxygen, Fe(II) and H2O2 concentrations. The presence of Fe(II) produced a rapid formation of Cu(I) in the first 2–3min of reaction. The Cu(I) is oxidized reaching a steady-state around 20nM levels of Cu(I). These experimental results demonstrated that the presence of Fe and Cu strongly affected the inorganic redox chemistry of both metals in UV-treated seawater.

Pollution in the Bay of Bengal: Impact on Marine Ecosystem

The study focused on heavy metal concentration in sea water, sea sediment and their toxic effect on sea shells, and on oyster along the east coast of the Bay of Bengal. The hierarchy of heavy metals in sea sediment of the bay showed as: Fe > Zn > Ni > Cr > Pb > Cd. The average concentration of nickel exceeded ERL (20.9 μg·g-1) value and the incidence of adverse effects on biological components exceeded 16.7%. The present study showed that the concentration of Cd, Fe, Pb and Cu in sea water were relatively higher than the standard concentration. The study showed a considerable amount of heavy metals (0.035%) such as Zn, Pb, Cu, Fe, and Mg in sea shells, and oyster which may make them toxic. The toxic effect of Pb and Zn may reduce their growth which is a great threat to marine ecosystem.

Synthesis, characterization and application of a novel ion-imprinted polymer for selective solid phase extraction of copper(II) ions from high salt matrices prior to its determination by FAAS

A new Cu(II)-imprinted sorbent has been prepared by using 5-methyl-2-thiozylmethacrylamide (MTMAAm). The monomer of Cu(II)–MTMAAm complex was synthesized and copolymerized in the presence of ethyleneglycol dimethacrylate cross-linker via bulk polymerization method. The resulting Cu(II)-imprinted polymer was characterized by FT-IR spectroscopy and scanning electron microscopy (SEM). Copper ions were removed from the polymer with 1.0molL−1 HNO3 and determined by flame atomic absorption spectrometry (FAAS). The imprinted polymer showed higher selectivity for Cu(II) in comparison to the non-imprinted polymer. Relative selectivity coefficients (k′) for Cu(II)/Zn(II), Cu(II)/Ni(II) and Cu(II)/Co(II) were 9.1, 14.8 and 26.6, respectively. The imprinted polymer was examined as a column packing material for solid phase extraction of Cu(II) from various matrices. The effects of solution pH, acid eluents and interfering ions were investigated. The poylmer possesses selective extraction of Cu(II) within pH range from 5.0 to 6.5. The relative standard deviation and limit of detection (3s) of the method were evaluated as 1.4% and 0.9µgL−1, respectively. The accuracy of the method was verified by analysis of two certified reference materials (CWW-TM-D and SRM 3280) and then applied to the determination of Cu in seawater, lake water and tap water samples, and hemodialysis concentrates and multivitamin/multielement supplements.