Crown Gall Tumor Cells Research Articles

Transfer of Ti plasmids between Agrobacterium strains by mobilisation with the conjugative plasmid RP4

The P type conjugative plasmid RP4 has been shown to be able to promote the transfer of the Agrobacterium Ti-plasmid. The results provide additional evidence that agrocin 84 sensitivity, exclusion of phage AP1, ability to catabolize the guanidine derivatives octopine and nopaline and tumor inducing ability, are Ti-plasmid determined properties. Furthermore, the results strongly support the notion that at least part of the Ti-plasmid is transferred from the bacterium to the target plant cells, since it was demonstrated that Ti-plasmid linked genes specify the synthesis of octopine or nopaline by crown-gall tumor cells.

Studies on the recovery of crown gall tumor cells

Our previous studies have shown that, while a persistent but potentially reversible suppression of the tumorous state appears to be a characteristic feature of the vegetative phase of teratoma shoot growth in the crown gall disease of plants, a recovery from that state occurs during the reproductive phase. An analysis has now been made of the reproductive process in an attempt to define the precise stage at which recovery occurs. The results of this analysis have shown that diploid somatic cells of teratoma-derived flower parts such as those found in petals and filaments are inherently neoplastic. On the other hand, haploid cells of plants grown from anthers of the same flowers and diploid cells of F(1) generation plants grown from teratoma-derived seed have, by generally accepted criteria, recovered from the tumorous state. These findings have been interpreted to mean that the loss of neoplastic properties occurs in crown gall teratoma cells during meiosis rather than during fertilization or later stages of the reproductive process.An analysis of more than 2000 teratoma-derived tumor shoots has shown, moreover, that a recovery from the tumorous state may also occur, although apparently as a very rare event, during the vegetative phase of teratoma shoot growth.

The Presence of Agrobacterium tumefaciens Plasmid DNA in Crown Gall Tumour Cells

SUMMARY: DNA hybridization studies indicate the presence of DNA complementary to Agrobacterium tumefaciens plasmid in bacterium-free crown gall tumour cells. The amount of this DNA is estimated to be about 0·1% of the tumour cell DNA.

High molecular weight deoxyribonucleic acid polymerase from crown gall tumor cells of periwinkle (Vinca rosea).

A high molecular weight (6 S) plant DNA polymerase from axenic Vinca rosea tissue culture cells has been purified 2200-fold and characterized. The enzyme has a molecular weight of 105 000 (+/-5000). Sodium dodecyl sulfate-acrylamide gel electrophoresis of the purified enzyme yields polypeptide subunits having molecular weights of 70 000 and 34 000. The purified enzyme has a pH optimum of 7.5; a cation requirement optimum of 6 mM Mg2+ or 0.5 mM Mn2+; an apparent requirement for Zn2+; a Km of 1 muM for dTTP; and a 3.5-fold stimulation by 50 mM KCl. The enzyme is sensitive to N-ethylmaleimide (1 mM), heparin (0.1 muM), ethanol (5%), pyrophosphate (0.05 muM), and o-phenanthroline (0.1 mM) but is insensitive to rifamycin. Denatured DNA is found to be the best natural template, and only negligible activity can be demonstrated with the ribopolymer templates poly(dT)n-poly(rA)n and p(dT)10-poly(rA)n. In addition to the polymerization reaction, the enzyme catalyzes a pyrophosphate exchange reaction. Antibody to calf thymus 6-8S DNA polymerase does not inhibit DNA polymerase from Vinca rosea, suggesting no antigenic relationships between the mammalian and plant enzymes.

Revised identification of the chromophore of a cell division factor from crown gall tumor cells of Vinca rosea L

Revised identification of the chromophore of a cell division factor from crown gall tumor cells of <i>Vinca rosea</i> L

Revised identification of the chromophore of a cell division factor from crown gall tumor cells of Vinca rosea L.

Low-resolution mass spectrometry, UV spectra in acid, neutral, and alkaline solution, and nuclear magnetic resonance indicated that the chromophore of one member of a new class of cell division-promoting factors isolated from crown gall tumor tissues consisted of a 3,7-alkyl-2-alkylthio-6-purinone with one free proton in the ring. Glucose constitutes the sugar moiety.

Liberation of agrobacterium tumefaciens dna from the crown gall tumor cell DNA from the crown gall tumor cell DNA by shearing

Unsheared DNA from normal or crown gall tumor tissue of tobacco and the sheared DNA from the normal tissue gave only one band (ϱ = 1.694) on CsCl density-gradient centrifugation. CsCl density-gradient centrifugation of sheared DNA from the tumor tissue, however, produced two bands, one corresponding to tobacco DNA (ϱ = 1.694) and the other corresponding to A. tumefaciens DNA (ϱ= 1.717). These results further strengthen the concept that crown gall tumor formation may involve integration of bacterial genome with the host cell genome.

DNA-DNA hybridization studies between bacterial DNA, crown gall tumor cell DNA and the normal cell DNA

DNA-DNA hybridization studies between bacterial DNA, crown gall tumor cell DNA and the normal cell DNA

Studies on the net uptake of solutes by normal and crown-gall tumor cells.

Studies on the net uptake of solutes by normal and crown-gall tumor cells.

Regulation of biosynthetic systems in normal and crown-gall tumor cells.

Transactions of the New York Academy of SciencesVolume 24, Issue 7 Series II p. 750-752 REGULATION OF BIOSYNTHETIC SYSTEMS IN NORMAL AND CROWN-GALL TUMOR CELLS* Henry N. Wood, Henry N. Wood The Rockefeller Institute, New York, N.Y.Search for more papers by this author Henry N. Wood, Henry N. Wood The Rockefeller Institute, New York, N.Y.Search for more papers by this author First published: May 1962 https://doi.org/10.1111/j.2164-0947.1962.tb01440.xCitations: 3 † This paper was the fourth of four papers presented at a joint meeting of the Section of Biological and Medical Sciences and the Division of Microbiology on April 9, 1962. The studies reported here were supported in part by grants No. E-159 and E-160 from the American Cancer Society, Inc. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume24, Issue7 Series IIMay 1962Pages 750-752 RelatedInformation

The Plant Tumor Problem

Publisher Summary This chapter focuses on the plant tumor problem. The tumor problem is a cellular problem and, at that level, no real fundamental differences exist between members of the two kingdoms. In comparing plant and animal tumors, it must be remembered that there are certain developmental and functional differences commonly used in the differentiation of animal cancers that are more or less restricted to animals and cannot be carried over and applied to plants. The first of the nonself-limiting neoplastic diseases of plants is known as Black's wound tumor disease. The second nonself-limiting tumorous disease of plants, known as the Kostoff genetic tumors, is one in which the genetic constitution of the host and, more particularly, all of the cells comprising the host play a primary role. The third nonself-limiting tumor disease is the crown-gall disease. Tumor formation takes place in essentially two distinct phases. In the first phase, normal cells are altered to tumor cells which do not, as yet, develop into a neoplastic growth. The second phase, according to this concept, is concerned with the continued abnormal and autonomous proliferation of the tumor cells once the cellular alteration has been accomplished. The rapid and consistent response of susceptible plant cells to transformation by a tumor-inducing principle in the crown-gall disease makes possible a very accurate seriation of events that occur during tumor inception. Studies dealing specifically with the biochemistry of the inception phase of tumor formation in the crown-gall disease are very limited in number and of recent origin. The abnormal histological and cytological picture observed in the plant tumor cells can be accounted for in terms of the abnormal growth hormone physiology, which is characteristic of the tumor tissue. Findings indicate that the crown-gall tumor cell contains both genetic and nongenetic factors that are present in a normal cell.

In vitro synthesis of the crown-gall tumour-inducing principle.

PREVIOUS research on crown-gall has shown that the transformation of normal plant cells into crown-gall tumour cells occurs in delimited stages involving specific causal agents1.

A Physiological Basis for Autonomous Growth of the Crown-Gall Tumor Cell

A Physiological Basis for Autonomous Growth of the Crown-Gall Tumor Cell

On the Role of Omega Bacteriophage in Formation of Crown-Gall Tumor Cells

Previous articleNext article No AccessLetters to the EditorsOn the Role of Omega Bacteriophage in Formation of Crown-Gall Tumor CellsRichard M. Klein and Robert E. BeardsleyRichard M. Klein Search for more articles by this author and Robert E. Beardsley Search for more articles by this author PDFPDF PLUS Add to favoritesDownload CitationTrack CitationsPermissionsReprints Share onFacebookTwitterLinkedInRedditEmail SectionsMoreDetailsFiguresReferencesCited by The American Naturalist Volume 91, Number 860Sep. - Oct., 1957 Published for The American Society of Naturalists Article DOIhttps://doi.org/10.1086/281996 Views: 4Total views on this site Citations: 11Citations are reported from Crossref PDF download Crossref reports the following articles citing this article:D. Owen, Kim Pearce, J. Hewitt, Julie Bryan, D. Coates, A. To, G. R. K. Sastry Studies on multiple drug resistance and its relationship to tumorigenicity in Agrobacterium tumefaciens B6-806, Archives of Microbiology 135, no.11 (Jan 1983): 42–44.https://doi.org/10.1007/BF00419480ARMIN C. BRAUN A History of the Crown Gall Problem, (Jan 1982): 155–210.https://doi.org/10.1016/B978-0-12-394380-4.50012-8U. C. Knopf Crown-Gall and Agrobacterium tumefaciens: Survey of a Plant-Cell-Transformation System of Interest to Medicine and Agriculture, (Jan 1979): 143–173.https://doi.org/10.1007/978-1-4615-7945-8_3I. Zaenen, N. van Larebeke, H. Teuchy, M. van Montagu, J. Schell Supercoiled circular DNA in crown-gall inducing Agrobacterium strains, Journal of Molecular Biology 86, no.11 (Jun 1974): 109–127.https://doi.org/10.1016/S0022-2836(74)80011-2W.P. Roberts, A. Kerr Crown gall induction: serological reactions, isozyme patterns and sensitivity to mitomycin C and to bacteriocin, of pathogenic and non-pathogenic strains of Agrobacterium radiobacter, Physiological Plant Pathology 4, no.11 (Jan 1974): 81–91.https://doi.org/10.1016/0048-4059(74)90047-2A. Kerr Acquisition of virulence by non-pathogenic isolates of Agrobacterium radiobacter, Physiological Plant Pathology 1, no.33 (Jul 1971): 241–246.https://doi.org/10.1016/0048-4059(71)90045-2M. Brunner, C. F. Pootjes Bacteriophage Release in a Lysogenic Strain of Agrobacterium tumefaciens, Journal of Virology 3, no.22 (Feb 1969): 181–186.https://doi.org/10.1128/jvi.3.2.181-186.1969R. M. Klein The physiology of bacterial tumors in plants and of habituation, (Jan 1965): 1856–1882.https://doi.org/10.1007/978-3-642-50088-6_46H. R. Hohl Über die submikroskopische Struktur hyperplastischer Gewebe von Datura stramonium L., Journal of Phytopathology 40, no.44 (Dec 1960): 317–356.https://doi.org/10.1111/j.1439-0434.1960.tb01929.xRobert E. Beardsley LYSOGENICITY IN AGROBACTERIUM TUMEFACIENS, Journal of Bacteriology 80, no.22 (Aug 1960): 180–187.https://doi.org/10.1128/jb.80.2.180-187.1960Tom Stonier AGROBACTERIUM TUMEFACIENS CONN I. Release of P 32and S 35 in Vitro, Journal of Bacteriology 79, no.66 (Jun 1960): 880–888.https://doi.org/10.1128/jb.79.6.880-888.1960

Necessity of Indoleacetic Acid for the Duplication of Crown-Gall Tumor Cells.

Necessity of Indoleacetic Acid for the Duplication of Crown-Gall Tumor Cells.

THE PROBABLE CHEMICAL NATURE OF CROWN‐GALL TUMOR‐INDUCING PRINCIPLE

IT HAS BEEN DEMONSTRATED that specific etiological agent-ttumor-inducing principle-is required as one of several agents and factors which, acting in sequence, transform normal cells into crown-gall tumor cells (Braun, 1947b). The temporal relation of tumor-inducing principle (TIP) to the transformation processes was investigated by Braun (1947a, 1952) and Braun and Mandle (1948), who showed that it was active subsequent to conditioning period and just prior to or during the first histologically detectable stages of wound healing. Thus, TIP begins acting on conditioned cells about 24 hr. after inoculation of crown-gall bacteria into healthy tissues. The period of its activity can be as short as 10 hr. under optimal conditions. Braun and Mandle (1948), in an extension of earlier studies (Riker, 1926), found that the principle apparently is destroyed or inactivated in vivo at 320C. at about the same rate as it is formed at 25?C. At 28?C. to 31?C. the rate of formation to TIP exceeded its rate of destruction. Braun (1950) reported that the activation energy for TIP inactivation was about 80,000 cal./mole and suggested that TIP or something intimately associated with its inactivation, may be . . . a factor of complex structure. Although conclusive data are lacking, it may be assumed that TIP is product of virulent crowngall bacteria synthesized by the bacteria in the host. The suppression of tumor cell formation by antibiotics (de Ropp, 1949) or by bacteriophage treatments of crown-gall bacteria (Brown and Quirk, 1929) supports this assumption. Klein and Klein (1953) showed that completely avirulent strains of crown-gall bacteria, as well as avirulent species of related bacteria, could acquire the property of virulence from nucleic acids produced by virulent forms and from the DNA isolated from virulent bacteria. This transmittance factor had no tumor-inducing ability and is not TIP. Once acquired, virulence appeared to be genetically fixed, indicating that hereditary units for virulence were acquired by bacteria deficient for these units (Cf. Lederberg, 1952). The formation of TIP by virulent bacteria thus seems to be conditioned by the presence and activity of hereditary units which regulate the production of the principle. Attenuated cultures of crowngall bacteria, on the other hand, appear to possess the ability to form TIP but apparently lack the abil-

Studies on the Metabolism of Plant Neoplasms

Studies on the Metabolism of Plant Neoplasms