Abstract
Unsheared DNA from normal or crown gall tumor tissue of tobacco and the sheared DNA from the normal tissue gave only one band (ϱ = 1.694) on CsCl density-gradient centrifugation. CsCl density-gradient centrifugation of sheared DNA from the tumor tissue, however, produced two bands, one corresponding to tobacco DNA (ϱ = 1.694) and the other corresponding to A. tumefaciens DNA (ϱ= 1.717). These results further strengthen the concept that crown gall tumor formation may involve integration of bacterial genome with the host cell genome.
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