Abstract
The pink-colored Cypridina luciferase (CypLase∗) from Cypridina (Vargula) hilgendorfii contains an unknown chromophore (CypL∗), derived from Cypridina luciferin (CypL). When CypLase∗ was treated with NAD(P)H-FMN flavin reductase (FRase) and NADH, the luminescence intensity in the reaction mixture increased significantly after gentle tapping. This observation suggests that CypL∗ in CypLase is enzymatically converted to CypL by the reduced flavin (FMNH2) through the FRase reaction, and the resulting complex of CypL and CypLase reacts with O2 to emit light. Therefore, CypL∗ might be a dehydrogenated form of CypL and might serve as another storage form in Cypridina specimens.
Published Version
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