Cross Interference Research Articles

A bivariate fluorescence biosensor based on Janus DNA nanoarchitecture-loaded dual-emissive Ag nanoclusters as bi-responsive signaling reporters

Constructing label-free bivariate fluorescence biosensor would be intriguing and desired for the recognizable and accurate detection of two specific DNA segments, yet the design of functional DNA structures with low overlapped interference might be challenging. Herein in this work, a double-faced Janus DNA nanoarchitecture (JDNA) with bi-responsive recognition regions on opposite sides was assembled, which consisted of two substrate strands and two template strands for loading green-/red-emissive Ag nanoclusters (gAgNC and rAgNC) as bivariate signaling reporters. Of note, the hybridized double helix in the middle rationally oriented two flank faces and stabilized the rigid conformation of JDNA, while the template sequences of bicolor clusters were blocked to minimize non-specific background leakage. Upon inputting two targets, the discernible hairpins lost their hairpin structures due to forming two dsDNA complexes. They were executed to simultaneously invade JDNA for activating two individual target-recycled strand displacement (TRSD) events, guiding signal transduction and efficient amplification. Consequently, the clustering templates were unlocked via the tailored conformation switch of JDNA, in which gAgNC and rAgNC were in situ synthesized in two diagonal positions, thereby significantly emitting bi-responsive signal without cross interference. Benefited from the logic integration of double-faced JDNA and TRSD, a label-free, sensitive and specific bivariate fluorescence approach was developed, which would open a new avenue for the potential application in biosensing and bioanalysis.

Click chemical ligation-enabled digital particle counting for multiplexed microRNA analysis

Digital counting assays, that quantify targets by counting individual signal entities, provide a promising way for the sensitive analysis of biomarkers even at the single-molecule level. Considering the requirements of complex enzyme-catalyzed amplification techniques and specialized instruments in traditional digital counting biosensors, herein, a simple digital counting platform for microRNA (miRNA) analysis is developed by employing the miRNA-templated click chemical ligation to hinge ultrabright quantum dot-doped nanoparticles (QDNPs) on the bottom of microplate well. Compared with the traditional short miRNA-mediated sandwich hybridization mechanism, the click chemistry-mediated ligation featured enhanced stability, achieving higher sensitivity by directly counting the number of QDNPs with a common wide-field fluorescence microscope. Furthermore, enzyme-free cycling click ligation strategy is adopted to push the detection limit of miRNA down to a low level of 8 fM. What is more, taking advantages of the tunable emission wavelength and narrow emission spectra of fluorescent nanoparticles, the platform enables simultaneous detection of multiplex miRNA targets without cross interference. Benefiting from the simple operation, high sensitivity, and good generality, miRNA analysis in complex samples is successfully achieved. This method not only pioneers a new route for digital counting assays but also holds great potential in miRNA-related biological researches.

Design of a mining carbon monoxide sensor and analysis of multi-gas cross-interference performance

Abstract A new type of carbon monoxide sensor is designed to address the issue of cross interference of nitrogen oxide gas in the exhaust emissions of underground diesel engine rubber-tired vehicles with electrochemical carbon monoxide sensors, which leads to frequent false alarm accidents. The hardware circuit design of the DC-DC voltage reduction power supply circuit, display circuit, component signal processing circuit, etc. is detailed, At the same time, the sensitive components used in the sensor adopt a special gas filtration membrane design. Six types of gases, including hydrogen sulfide, nitric oxide, nitrogen dioxide, hydrogen, ethylene, and acetylene, were sequentially introduced in the laboratory for experimental analysis of multi-gas cross-interference performance. The experimental results have shown that the new carbon monoxide sensor can filter out nitrogen oxide gas, thereby solving the impact of underground rubber tire vehicle exhaust on the accuracy of carbon monoxide gas detection. It has broad prospects for promotion and application in coal mines underground.

Research on the preparation of multi-core photonic crystal fiber and the curvature sensing performance of three-core photonic crystal fiber

Two- and three-core photonic crystal fibers are prepared by using the same photonic crystal fiber preform structure. Not only the fiber diameter is reduced to standard size of 125μm, but also the core diameter is reduced to below 4μm. A curvature and temperature sensing system is built, and the curvature and temperature sensing performance of the three-core optical fiber is tested. When the length of three-core optical fiber is 150 mm, the measured curvature sensitivity is −7.27069 nm/m−1 in the range of 0 to 0.990 m−1, and the temperature sensitivity is −34 pm/°C during the temperature reduction from 75 to 45 °C. When the length of three-core photonic crystal fiber is 68 mm, the curvature sensitivity and temperature sensitivity are 2.42419 nm/m−1 and −7 pm/°C, respectively. In a small range of temperature, the sensor is insensitive to temperature, and the cross interference of temperature to curvature is very small.

Rapid Photoacoustic Exhaust Gas Analyzer for Simultaneous Measurement of Nitrogen Dioxide and Sulfur Dioxide.

A rapid photoacoustic (PA) exhaust gas analyzer is presented for simultaneous measurements of nitrogen dioxide (NO2) and sulfur dioxide (SO2). A laser diode (LD) emitting at 450 nm and a light-emitting diode (LED) with a peak wavelength of 275 nm operated simultaneously, producing PA signals of NO2 and SO2, respectively. The LD and LED were modulated at different frequencies of 2568 and 2570 Hz, and their emission light beams were transmitted through two resonant tubes in a differential PA cell (DPAC), respectively. A self-made dual-channel digital lock-in amplifier was used to realize the simultaneous detection of dual-frequency PA signals. Cross interference between the PA signals at the two different frequencies was reduced to 0.02% by using a lock-in amplifier. In order to achieve a rapid dynamic measurement, gas sampling was accelerated by an air pump. The use of mufflers and the differential PA detection technique significantly reduced the gas sampling noise. When the gas flow rate was 1000 sccm, the response time of the PA dual-gas analyzer was 8 and 17 s for NO2 and SO2, respectively. The minimum detection limits of NO2 and SO2 were 1.7 and 26.1 ppb when the averaging time of the system was 10 s, respectively. Due to the wide spectral bandwidth of the LED, NO2 produced an interference to the detection of SO2. The interference was reduced by the precise detection of NO2. Since the radiations of the LD and LED passed through two different PA tubes, the impact of NO2 photochemical dissociation caused by UV LED luminescence on NO2 gas detection was negligible. The sharing of the PA cell, the gas lines, and the signal processing modules significantly reduced the size and cost of the PA dual-gas analyzer.

Potential-Resolved Ratiometric Aptasensor for Sensitive Acetamiprid Analysis Based on Coreactant-free Electrochemiluminescence Luminophores of Gd-MOF and "Light Switch" Molecule of [Ru(bpy)2dppz]2.

For conventional potential-resolved ratiometric electrochemiluminescence (ECL) systems, the introduction of multiplex coreactants is imperative. However, the undesirable interactions between different coreactants inevitably affect analytical accuracy and sensitivity. Herein, through the coordination of aggregation-induced emission ligands with gadolinium cations, the self-luminescent metal-organic framework (Gd-MOF) is prepared and serves as a novel coreactant-free anodic ECL emitter. By the intercalation of [Ru(bpy)2dppz]2+ with light switch effect into DNA duplex, one high-efficiency cathodic ECL probe is obtained using K2S2O8 as a coreactant. In the presence of acetamiprid, the strong affinity between the target and its aptamer induces the release of [Ru(bpy)2dppz]2+, resulting in a decreasing cathode signal and an increasing anode signal owing to the ECL resonance energy transfer from Gd-MOF to [Ru(bpy)2dppz]2+. In this way, an efficient dual-signal ECL aptasensor is constructed for the ratiometric analysis of acetamiprid, exhibiting a remarkably low detection limit of 0.033 pM. Strikingly, by using only one exogenous coreactant, the cross interference from multiple coreactants can be eliminated, thus improving the detection accuracy. The developed high-performance ECL sensing platform is successfully applied to monitor the residual level of acetamiprid in real samples, demonstrating its potential application in the field of food security.

A Decoupled Multichannel Based Wireless SRM System With Tunable Compensation Network and Multifrequency Pulse Density Control

This paper proposes and implements a decoupled multichannel based wireless switched reluctance motor (WSRM) system with tunable compensation network (TCN) and multifrequency pulse density control (MPDC). In previous studies, several wireless motor systems have been proposed. However, there are still some issues such as cross interference, power balance and dynamic speed regulation that are not well considered. The proposed system firstly suppresses the cross interference between receivers via the resonant filters to reduce the coupling between multiple power channels. Then, the switch-controlled capacitor (SCC) are employed to implement a tunable compensation network to ensure power balance and load-independent output characteristics under multiple operation frequencies. Additionally, the speed regulation is realized by the proposed MPDC strategy, which achieves the commutation by switching the operation frequency and energizes the phase winding by adjusting the pulse density of the inverter. Finally, a 120 W prototype with 36 V input voltage is constructed and evaluated. The experimental results validate the improvement of the decoupled power channel and the feasibility of the proposed speed control method. The steady-state and dynamic performance of the proposed system are both tested. The efficiencies and losses of the system are analyzed and the peak efficiency of 83.58% is reached.

Quantitative, multi-species gas sensing using broadband terahertz time-domain spectroscopy

The broadband terahertz wave, with its correspondence to the fingerprint spectra of gas molecules and relatively high transmittance through smoke, aerosol, and combustion environments, bears great potential for gas detection and combustion diagnostics. While access to rotational spectral fingerprints in the terahertz region provides opportunities for species-selective diagnostics with minimized background and cross interference, few studies have been devoted to direct, quantitative, and simultaneous analysis of multiple components exploiting the terahertz region. In this work, we achieve quantitative measurements of CO, NH3 and H2O gas concentrations at standard temperatures and pressures over a bandwidth of 1 THz, using direct absorption spectrum from femtosecond-laser-based terahertz time-domain spectroscopy. Spectral fitting of the fully resolved rotational lines yields good precision and accuracy with validation against calibrated mixtures. The estimated detection limits of the multi-species sensing system are 250 ppm m, 7 ppm m and 4 ppm m for CO, NH3 and H2O, respectively. The demonstration of quantitative, multi-species gas sensing indicates the feasibility and practical value of using broadband terahertz absorption spectroscopy for real-time, quantitative analysis and speciation of multicomponent gases in complicated practical environments such as combustion and multi-phase flows.

A novel fluorescent probe for the detection of Fe3+ ions with Eu3+/SnO2 nanocrystals Co-doped silica glasses

Detection of Fe3+ ions is of paramount importance for environmental protection and human health. Herein, we reported a highly selective and sensitive fluorescence probe for the quantification of Fe3+ ions in aqueous solution with Eu3+/SnO2 nanocrystals co-doped silica glasses as the sensing unit. Due to the high stability of silica glasses and their efficient protection for emitting centers, the probe has good recyclability and can be applied in real water environment, indicating its potential for on-site sensing. The detection limit of Fe3+ ions is 7.54 nmol/L. Experiments were carried out to verify that the probe shows excellent cross interference with other metal ions. The detecting mechanism can be ascribed to the excitation/emission absorption and energy transfer of Fe3+ ions. Our work paves a novel way of semiconductor oxide nanocrystals for applications in optoelectronics.

Redox Behavior of Arsenic (III) and Copper (II) Mixtures on Ultraflat Au (111) Thin Film Electrodes

The electrochemical behavior of arsenic on gold (Au) electrodes surfaces is of great interest as As is well-recognized as a highly toxic and relatively ubiquitous ground water species. The United States Environmental Protection Agency (USEPA) and World Health Organization (WHO) drinking water standards for arsenic are 10 PPB, or 𝜇g L-1 [1]. These minute ion concentrations necessitate the use of analytical methods with high sensitivity and a low susceptibility to cross interferences. Electrochemical stripping voltammetry is a well-established electroanalytical method for arsenic ions [2]. Polycrystalline gold electrodes are often used due to their high redox stability and relatively low overpotential for hydrogen evolution. We recently explored the use of ultra-flat, thin film, Au(111) electrodes to replace polycrystalline Au for As stripping voltammetry. Previous studies on oriented single crystals showed relatively narrow stripping peaks with greatly improved peak to background ratios on the (111) surfaces [3]. We observed comparatively similar or better stripping behavior on the Au(111) thin film electrodes with correspondingly far less gold usage per electrode. Unfortunately, copper is a known interference for arsenic stripping electroanalysis, with a strong affinity for UPD deposition on gold. We therefore studied the deposition behavior of Cu on Au(111) thin film electrodes and the co- deposition of Cu and As on the Au(111) thin film electrodes to evaluate cross interference effects. Copper redox behavior in 0.5M H2SO4 was characterized by cyclic voltammetry, electro- deposition and linear stripping voltammetry (LSV) measurements. Strong Cu UPD behavior was observed on Au(111) thin films with sharper, better defined redox peaks than polycrystalline electrodes. Arsenic and Cu ions were also co-deposited over a wide range of Cu (II) and As (III) concentrations. Complex redox behavior was observed with strong indications of Cu-As alloy formation. This was confirmed via angle resolved, X-ray Photoemission Spectroscopy (XPS) measurements. Our results also indicate that the electrodeposition efficiency during LSV of As is increased by the co-deposition of Cu. The results of this study have important implications for understanding the nature of Cu (II) interference with electrochemical detection of trace As (III) in water.[1] USEPA. National Secondary Drinking Water Regulations. 2009. https://www.epa.gov/sdwa/drinking-water- regulations-and-contaminants (accessed.[2] USEPA. METHOD 7063: ARSENIC IN AQUEOUS SAMPLES AND EXTRACTSBY ANODIC STRIPPING VOLTAMMETRY (ASV). 1996.[3] Casuse-Driovínto, T. Q.; Rizo, R.; Benavidez, A.; Brearley, A. J.; Cerrato, J. M.; Garzon, F. H.; Herrero, E.; Feliu, J. M. Increased Sensitivity and Selectivity for As(III) Detection at the Au(111) Surface: Single Crystals and Ultraflat Thin Films Comparison. The Journal of Physical Chemistry C 2022, 126 (48), 20343-20353. DOI: 10.1021/acs.jpcc.2c05541.

Study on preparation and curvature sensing performance of dual-core photonic crystal fibers

A method of fabricating dual-core photonic crystal fiber(PCF) using secondary drawing technique is presented. The diameter of fiber core can be reduced to 4μm by placing the thin preform into glass sleeve for secondary drawing. Dual-core PCF, partial dual-core PCF and non-centrosymmetric dual-core PCF are successfully prepared by secondary drawing technology combined with air pressure regulation. A curvature sensing system is built. When the length of partial dual-core PCF is 105 mm, the measured curvature sensitivity is −3.79944 nm/m−1. Through the water bath temperature experiment, the measured temperature sensitivity is 21 pm/∘C, and the cross interference caused by temperature value on curvature value is very small. When the length of non-centrosymmetric dual-core PCF is 207 mm, the curvature sensitivity measured by forward bending and negative bending is 4.68429 nm/m−1 and −4.68808 nm/m−1, respectively. The measured temperature sensitivity is −13 pm/∘C, and the cross interference of temperature on curvature can be ignored.

Anti-interference detection of mixed NOX via In2O3-based sensor array combining with neural network model at room temperature

Herein, we report a noble metal doped In2O3-based sensor array applying back propagation neural network (BPNN) combined with whale optimization algorithm (WOA) toward anti-interference detection of mixed NOX. The synthesis (simple hydrothermal methods) and characterization (XRD, SEM, EDS and XPS) of Pt, Au and Pd doped In2O3 with different morphologies were reported. The three of In2O3-based sensors were systematically tested at room temperature to investigate the performance of sensitivity, response-recovery time, repeatability and selectivity to NO and NOX. Based on the sensor array composed of Pt, Au, and Pd doped In2O3 sensors combining WOA-BPNN prediction model, this work finally achieved the quantitative prediction of the components in the mixed NO and NOX under the influence of cross interference.

Distance-Regulated Photoelectrochemical Sensor "Signal-On" and "Signal-Off" Transitions for the Multiplexed Detection of Viruses Exposed in the Aquatic Environment.

Photochemical (PEC) sensors were severely limited for multiplex detection applications due to the cross interference between multiplex signals at the single recognition interface. In this work, a distance-regulated PEC sensor was developed for multiplex detection by using an i-Motif sequence with conformational transformation activity as the signal transduction unit. Through dynamic regulation of the spatial distance between the end site of the functional sequence and the electrode material, the photogenerated electrons on the surface of the sensor were directionally transferred. Thus, a PEC sensor with "signal-on" and "signal-off" dual signal output modes was developed for simultaneous detection of multitarget molecules. Combining isothermal nucleic acid amplification, the PEC sensor constructed in this work was successfully applied to the detection of two virus (Norovirus and Rotavirus) nucleic acid sequences. Under the optimal condition, this bioassay protocol exhibits a linear range of 0.01-100 nM for both viruses with detection limits of 0.72 and 0.53 pM, respectively. In this study, a stimulus-mediated distance regulation strategy successfully addressed the transduction of multiplex detection signals at the single recognition interface of the PEC sensor. It is expected that the technical barriers to multiplex detection of PEC sensors will be overcome and the application of PEC sensing technology will be expanded in the field of environmental analysis.

A new approach for determination of orthophosphate based on mixed valent molybdenum oxide/poly 1,2-diaminoanthraquinone in seawater

Orthophosphate is an essential macronutrient in natural water that controls primary production and strongly influences the global ocean carbon cycle. Electrochemical determination of orthophosphate is highly recommended because electrochemistry provides the simplest means of determination. Here the determination of orthophosphate based on the formation of a phosphomolybdate complex is reported. Mixed-valent molybdenum oxide (MoxOy) was prepared by cyclic voltammetry on poly-1,2-diaminoanthraquinone (1,2-DAAQ), which was performed by cyclic voltammetry on the surface of a glassy carbon electrode under pre-optimized conditions for the thickness of the modified electrode layers. The proposed modified electrode was used for square-wave voltammetry of orthophosphate ions under pre-optimized square-wave parameters (i.e., frequency and amplitude) in strongly acidic medium (pH < 1). The linear range was 0.05–4 µM with a limit of quantification (LOD) of 0.0093 µM with no effect on two peaks due to cross interference from silicate. Furthermore, MoxOy/PDAAQ shows good reproducibility with a relative standard deviation (RSD) of 2.17% for the peak at 0.035 V and 3.56% for the peak at 0.2 V. Real seawater samples were also analyzed for PO43− analysis by UV spectrophotometry and the results were compared with the measurement results of our proposed electrode, with good recoveries obtained.

Potential-resolved electrochemiluminescence biosensor for simultaneous determination of multiplex miRNA

The multi-target simultaneous detection strategy based on potential-resolved electrochemiluminescence (ECL) has still been a research hotspot in analytical science, but the limited selection of ECL luminophores hinders the development of this field. Herein, polyethyleneimine functionalized perylene derivatives (PTC-PEI) and luminol functionalized gold nanoparticles (Lu–Au NPs) possessed significantly resolved emission potentials as ECL luminophore. The ternary ECL system was constructed with MoS2 nanoflowers and K2S2O8 as the coreaction accelerator and coreactant respectively, which significantly improved the cathode ECL emission of PTC-PEI. Simultaneously, the anode coreaction accelerator ZnO nanoflowers could promote the anode coreactant dissolved O2 reduction, and extremely enhanced the anode ECL emission of Lu–Au NPs. The proposed strategy addressed the major technical challenge of cross interference and competition of the coreactants for dual-biomarker detection, thus enabling accurate detection of miRNA-205 and miRNA-21 from 10 fM to 100 nM, with detection limits of 2.57 and 1.15 fM, respectively. In general, this work achieved a single-step synchronous detection of dual biomarkers, providing a new idea for the ECL detection of multiple biomarkers, and having potential value in the clinical diagnosis.

Electrochemical Sensors Based on Manganese and Cobalt Oxide Nanostructures for the Detection of Flutamide and its Derivatives in Real Water Samples

AbstractFlutamide (FLU), bicalutamide (BIC), and hydroxyflutamide (OHF), having a low biodegradability, may cause severe health effects on humans as antiandrogens. In this work, we have developed two electrochemical sensors using manganese oxide (MnO) and Cobalt oxide (CoO) nanostructures (NSs) as electrocatalysts. The GCE modified with MnO is referred to as MnO/GCE and the GCE modified by CoO is referred to as CoO/GCE. The electrochemical behaviours of CoO/GCE and MnO/GCE were examined in ferricyanide solution. It was observed through the employment of cyclic voltammetry that MnO/GCE exhibit better electron transfer than CoO/GCE. The calculated surface coverage values, 1.46 x10−9 mol cm−2 and 5.02 x10−9 mol cm−2 of MnO/GCE and CoO/GCE suggest a multilayer of a metal oxide molecule film at the surfaces of glassy carbon electrodes (GCE). FLU, BIC and OHF were detected at a linear range from 32.01 to 50.00 µM. The limits of detection of FLU, BIC and OHF were 18.5, 13.0 and 78.8 µM at MnO/GCE respectively and 18.8, 18.7 and 18.5 µM at CoO/GCE respectively. Both MnO/GCE and CoO/GCE showed good catalytic stability towards detecting FLU and its derivatives. FLU, BIC and OHF were also detected in the presence of interferents for both electrochemical sensors in phosphate buffer solution. Both MnO/GCE and CoO/GCE confirmed good selectivity without cross interference. Some of the health effects associated with FLU, BIC and OHF are liver damage, prostate inflammation, and methamoglobenia. Although FLU, BIC and OHF are detected in low concentration levels in water bodies, their continuous ingestion is a great concern. As far as we know, MnO and CoO NSs have not been used to electrochemically detect FLU, BIC and OHF. Furthermore, OHF has not been detected electrochemically before and there are only a few studies on the electrochemical detection of BIC. Hence, MnO and CoO NSs are used in this study for the first time for an electrochemical sensor fabrication towards the detection of FLU, BIC and OHF.

Detection of gaseous halocarbon refrigerants and extinguishing agent based on photoacoustic spectroscopy

A photoacoustic (PA) gaseous halocarbon refrigerants and extinguishing agent detector based on a mid-infrared broadband source was developed. H1301 (extinguishing agent), R134a and R410A (refrigerants) were the target gases. A mid-infrared thermal radiation source radiated along the longitudinal direction of the cylindrical PA cell and was reflected by a gold-plated mirror to excite PA signals. The concentrations of CO2 and H2O were measured to reduce their interference. The PA signals of H1301, R134a, R410A, CO2 and H2O were excited by using a thermal radiation source with five different optical filters, respectively. The fundamental-frequency intensity modulation (1f-IM) technique was used to measure the generated PA signals. The detection limits (1σ) of H1301, R134a and R410A were 49 ppb, 80 ppb and 211 ppb with an average time of 100 s, respectively. According to the absorption spectrum of the three target gases, there are strong cross interference around the wavelength of 8.5 µm. By using the proposed calculation method to obtain the concentrations of the target gases, the high cross interference among these gases is reduced to less than 2 %.

A Mid-Infrared Quantum Cascade Laser Ultra-Sensitive Trace Formaldehyde Detection System Based on Improved Dual-Incidence Multipass Gas Cell

Formaldehyde (HCHO) is a tracer of volatile organic compounds (VOCs), and its concentration has gradually decreased with the reduction in VOC emissions in recent years, which puts forward higher requirements for the detection of trace HCHO. Therefore, a quantum cascade laser (QCL) with a central excitation wavelength of 5.68 μm was applied to detect the trace HCHO under an effective absorption optical pathlength of 67 m. An improved, dual-incidence multi-pass cell, with a simple structure and easy adjustment, was designed to further improve the absorption optical pathlength of the gas. The instrument detection sensitivity of 28 pptv (1σ) was achieved within a 40 s response time. The experimental results show that the developed HCHO detection system is almost unaffected by the cross interference of common atmospheric gases and the change of ambient humidity. Additionally, the instrument was successfully deployed in a field campaign, and it delivered results that correlated well with those of a commercial instrument based on continuous wave cavity ring-down spectroscopy (R2 = 0.967), which indicates that the instrument has a good ability to monitor ambient trace HCHO in unattended continuous operation for long periods of time.

Development and validation of LC-MS/MS methods for the quantification of 101BHG-D01, a novel, long-acting and selective muscarinic receptor antagonist, and its main metabolite M6 in human plasma, urine and feces: Application to a clinical study in healthy Chinese subjects

101BHG-D01 is a novel, long-acting and selective muscarinic receptor antagonist for the treatment of chronic obstructive pulmonary disease (COPD) and rhinorrhea in rhinitis. To support its clinical study, several liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for the quantification of 101BHG-D01 and its main metabolite M6 in human plasma, urine and feces were developed. The plasma samples were prepared by protein precipitation, and the urine and fecal homogenate samples were pretreated by direct dilution, respectively. The chromatographic separation was performed on an Agilent InfinityLab Poroshell 120 C18 column with 0.1% formic acid and 10.0 mM ammonium acetate buffer solution in water and methanol as the mobile phase. The MS/MS analysis was performed by using multiple reaction monitoring (MRM) under a positive ion electrospray ionization mode. The methods were validated with regards to selectivity, linearity, lower limit of quantitation (LLOQ), accuracy and precision, matrix effect, extraction recovery, dilution integrity, batch size, carryover and stability. The calibration ranges were as follows: 1.00–800 pg/mL for 101BHG-D01 and 1.00–20.0 pg/mL for M6 in plasma; 0.0500–20.0 ng/mL for 101BHG-D01 and M6 in urine; 0.400–400 ng/mL for 101BHG-D01 and 0.100–100 ng/mL for M6 in feces. There was no endogenous or cross interference observed at the retention time of the analytes and internal standard in various biological matrices. Across these matrices, for the lower limit of quantitation quality control (LLOQ QC) samples, the intra- and inter-batch coefficients of variation were within 15.7%. For other QC samples, the intra- and inter-batch coefficients of variation were within 8.9%. The intra- and inter-batch accuracy deviations for all QC samples were within the range of − 6.2–12.0%. No significant matrix effect was observed from the matrices. The extraction recoveries of these methods at different concentrations were consistent and reproducible. The analytes were stable in different matrices under various storage conditions. The other bioanalytical parameters were also fully validated and met the criteria given in the FDA guidance. These methods were successfully applied to a clinical study in healthy Chinese subjects after a single dose administration of 101BHG-D01 inhalation aerosol. After inhalation, 101BHG-D01 was absorbed into plasma rapidly with the time to reach the maximum drug concentration (Tmax) of 5 min and eliminated slowly with a half-life time about 30 h. The cumulative urinary and fecal excretion rates revealed 101BHG-D01 was mainly excreted in feces, rather than urine. The pharmacokinetic results of the study drug laid a foundation for its further clinical development.

Ionic Gel Based Multifunctional Sensor for Body Temperature Monitoring and Joint Motion Detection

Flexible temperature and strain sensors are key components of wearable devices for healthcare monitoring. Currently, the insufficient precision, undesirable mechanical properties, and the cross interference between different external stimuli are still bottleneck problems for flexible sensors especially in body temperature and human motion monitoring. Herein, an ionic gel based on ionic liquids (ILs) and commercial thermoplastic polyurethane (TPU) fiber is fabricated via a feasible and scalable immersion method for temperature and strain multifunctional sensing. Wearable sensor based on ionic gel exhibits high temperature sensitivity (2.73% ◦C−1) and sufficient resolution (0.1 ◦C) in the skin temperature range. Meanwhile, the sensor exhibits linear and stable strain sensing performance and has the ability to respond to tiny strains. More importantly, the multifunctional fiber‐like sensor is easy to integrate with traditional textile, which is beneficial to solve the wearability problems such as discomfort and impermeability in practical applications. Notably, the stimulus caused by strain can be almost avoided by sewing smart fibers into S‐shape during the temperature monitoring, which helps decouple the temperature and strain signals. As prepared intelligent textiles can accurately capture small fluctuations in temperature and detect the action amplitude of the human body, which is important for disease prediction and rehabilitation training of patients.