Highlights The response of dicamba-susceptible soybean to three simulated rates and growth stages has been studied. Soybean physiological data, such as plant height and phototoxicity, was collected at the selected growth stage. Aerial images of the experiment field using UAV were collected on specific days after treatment. Soybean yield, biological response, and the correlation of vegetative indices in predicting soybean yield were achieved. Abstract. Many dicotyledonous weeds can be selectively controlled by dicamba application during the growing season. However, vapor or spray drift from dicamba application could cause significant yield loss on non-target species such as soybean [Glycine max (L.) Merrill] that do not contain the dicamba resistance trait. In this study, the impact of simulated dicamba drift was investigated on dicamba-susceptible soybeans during different growth stages. A field experiment was conducted employing small unmanned aircraft systems (sUAS) and multispectral image sensors to assess the dose-response relationship between dicamba drift and soybean yield. The experiment followed a randomized complete block design, with main plots representing three distinct soybean growth stages (third trifoliate, sixth trifoliate, and early reproductive stage) and sub-plots encompassing various simulated dicamba drift rates. The analysis, involving non-linear regression and normalized difference vegetation index (NDVI) regression, revealed that visible soybean injuries were observed at minimal dicamba rates. However, significant yield loss occurred during the V6 growth stage at a dicamba rate of 98 g.ai ha-1, emphasizing the critical growth stage sensitivity. Moreover, a moderate yet meaningful relationship (R2 = 0.46) was established between sUAS multispectral NDVI data and soybean yield, highlighting the potential for sUAS with multispectral sensors to predict soybean yield losses. These results contribute valuable insights into the impact of dicamba drift on soybeans and the efficacy of remote sensing technology in assessing yield outcomes. Keywords: Dicamba-drift injury, Multispectral image, Precision agriculture, Soybean yield, sUAS, Yield prediction.
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