Blood Of Cows Research Articles

Relationships Between Nutrient Of Land And Cow’s Blood Added With Bioactivators On Performance of Soybean (Glycine max (L.) Merr.)

Soybean production in Bengkulu Province is still lower than national production. Soybean production can still be improved by adding nutrients, one source of untapped nutrients is cow's blood which is still a waste. The purpose of this research is to determine the effect of cow’s blood added with bioactivators on growth performance of soybean. This research was conducted by using a complete randomized design (CRD) arranged factorially consisted of two factors with three replications. The first factor was the various kinds of cow’s blood added with bioactivators consisting of Yeast, Stale Rice, Microorganism-Bio(M-Bio), Effective Microorganism-4 (EM-4), and Rumen. The second factor was the concentration of cow’s blood added with bioactivators consisting of 15%, 30%, and 45%. Results showed: 1) There was no treatments interaction between various kinds of cow’s blood added with bioactivators and its concentration in the growth, 2) the various kinds of cow’s blood added with bioactivators had significant effect in the growth of plant height, number of leaves, number of branches, wet and dry weight, but it had no significant effect on plant height and leaf area, and 3) The concentration of cow’s blood added with bioactivators has no significant effect in the growth. This study indicated that cow’s blood added with Rumen as bioactivator showed better growth performance of soybean. Keywords: bioactivators, cow’s blood, nutrient of land, performance of soybean

Результати морфологічного дослідження крові корів за теплового стресу залежно від стадій температурно-вологісного індексу

The results of morphological studies of the blood of cows under heat stress indicate that the temperature - humidity index (THI) of animals in comfortable conditions (THI - 68-71) the number of erythrocytes is 5.78 ± 0.13 T / l, the hematocrit - 24.98 ± 1.02%, hemoglobin content - 89.96 ± 2.63 g / l, MCV - 39.76 ± 2.31 fl; MCH - 15.56 ± 1.32 pg; MCHC - 39.14 ± 1.95 g / dl. The total number of leukocytes in the blood of cows is 7.17 ± 0.54 G / l. The relative content (leukogram) and the absolute number of certain types of leukocytes in the blood of cows has no abnormalities. This suggests that the morphological parameters of the blood of cows in the comfortable conditions of THI meet the normative values for cattle and, in particular, Holstein breed. Under moderate heat stress (THI -72-78), in the blood of cows, along with a significant increase in respiratory rate and heart rate, there is a tendency to increase the number of erythrocytes to 5.82 ± 0.69 T / l, there is a significant increase in hematocrit and MCV by 20 and 47%, and a significant decrease in hemoglobin, MCH and MCHC - by 32, 35 and 55%, respectively, relative to control. However, in the blood of cows there is a tendency to decrease the number of leukocytes, with the simultaneous appearance of metamyelocytes, an increase in the number of eosinophils and rod neutrophils, and a significant decrease in the number of lymphocytes by 27% compared to control. Under severe heat stress (THI - 80-90), when the pulse in cows fluctuates between 88-140 beats per minute, and the respiratory rate is 82.0 ± 3.76 respiratory movements, the number of erythrocytes in the blood of cows tends to decrease and is 5.01 ± 0.91 T / l. The hematocrit value is 21% significantly (p≤0.05) less than in cows under moderate heat stress, and is almost the same as in cows under comfortable conditions. The content of hemoglobin in the blood of cows in this period is significantly lower by 28% (p≤0.05) compared to that in cows under comfortable conditions with a slight tendency to increase compared to this figure in cows under moderate heat stress. Color indices indicate that in the blood of cows under severe heat stress there is a large number of young erythrocytes-macrocytes (MCV - 53.69 ± 3.22 fl), which are poor in hemoglobin (MSN - 12.95 ± 3.48 pg) and impaired hemoglobin formation (MCHC - 24.11 ± 2.23 g / dl). The total number of leukocytes in the blood of cows under severe heat stress is 10.80 ± 1.55 G / l, which is 78% (P≤0.01) more than under moderate heat stress and increases due to the appearance of young and an increase of almost in twice rod-shaped and segment-nuclear neutrophils and eosinophils with a simultaneous decrease in the percentage of lymphocytes.

Взаємозв’язок основних характеристик нервових процесів із вмістом натрію у крові корів залежно від пори року

The type of higher nervous activity determines individual differences and the body's ability to adapt to changing environmental conditions. The most perfect adaptation is provided by a combination of high strength, mobility and balance of nervous processes. It was found that the strength of nervous processes both in summer and in winter was inversely related only to the sodium content in blood cells (r = -0.57–0.71; p<0.01). The balance of excitation and inhibition processes in the cerebral cortex in summer was inversely correlated with the sodium content in whole blood (r = -0.56; p<0.05) and blood cells (r = -0.64; p <0.01), in winter – in blood serum of cows (r = 0.62; p <0.01). It is proved that in summer the effect of the balance on the sodium content in whole blood was ղ²х = 0.29 (p <0.05), in blood cells – ղ²х = 0.63 (p<0.001), and in winter in blood cells – ղ²х = 0.37 (p<0.05). In summer, the mobility of nervous processes to a greater extent limited the sodium content in blood of cows than in winter. Thus, the effect of this indicator of cortical regulation on the sodium content in whole blood and blood cells in summer reached ղ²х = 0.25–0.35 (p<0.05), and in winter in blood cells – ղ²х = 0.24 (p<0.05).

The intensity of the processes of lipid peroxidation in the blood of cows in experimental fasciolosis

Among the most common pathologies of parasitic etiology in ruminants, trematode diseases, namely fasciolosis, occupy a prominent place in terms of the degree of damage and economic damage caused. Activation of lipid peroxidation processes plays a significant role in the mechanisms of the development of fasciolosis in animals. The research aimed to investigate the level of lipid peroxidation products in cows' blood in experimental fasciolosis. For the test, 12 cows of 4–5 years of age, black-spotted breed, were selected, from which 2 groups were formed, six animals in each. Animals in the control group were clinically healthy. Animals of the experimental group were experimentally infected with adolescents. During the research, the rules of compulsory experiments were followed – selecting and keeping analog animals in groups. The cows' diet was balanced in terms of nutrients and minerals. Based on the studies, it was found that after the experimental infestation of cows of the experimental group with the causative agent of fasciolosis, an increase in the level of diene conjugates of their blood on the 15th day of the experiment by 30.3 %, and on the 25th day – by 50.4 % relative to the control group. There was also an increased level of lipid hydroperoxides in their blood, where, respectively, on the 10th and 15th day of the experiment, this figure increased by 18.3 and 32.9 % compared with the control group. On the 25th day of the experiment, the level of lipid hydroperoxides in the blood of cows of the experimental group was the highest. Compared with the control group, it increased by 46.6%, respectively. In the study of the final products of lipid peroxidation, it was found that on the 10th day of the test, the level of TBA-active products in infected cows of the experimental group increased by 9.5 %. On the 15th and 20th days of the experiment, the level of end products of LPO in the experimental group's blood of animals was higher by 20.3 and 26.0 % relative to the control group. The increase in TBA-active products' level reflects the activation of peroxide processes in animals with experimental fasciolosis. Therefore, in the clinical manifestation of fasciolosis, parasites secrete metabolic products that promote the formation of free radicals, which in turn enhance the initiation of lipid peroxidation processes.

Draft Genome Sequence of Serratia marcescens Strain ZZCCN01, Isolated from the Cardiac Blood of a Beef Cow.

Serratia marcescens strain ZZCCN01 was isolated from the cardiac blood of a dead beef cow with a lung infection and a foam-like secretion from the nostril. Here, we introduce the 5.1-Mb draft genome sequence, which comprises 105 scaffolds, and the corresponding annotation.

Association and heritability of traits of milk productivity and blood in the dairy cows

Numerous studies have shown that there is a certain association between the biochemical parameters of blood and milk. It is of big practical importance to determine the heritability of traits involved in the selection process, which gives breeders the opportunity to choose the direction of selection for the fastest solution of the tasks. The purpose of the researches was to establish the association between the indicators of the biochemical composition of milk and blood in the dairy cows, as well as to determine the heritability of the studied traits. It has been found in the course of researches that in most cases the correlations between the biochemical parameters of blood and milk were insignifi cant and unreliable. It can be seen that there is a slight correlation between the main indicators of blood and milk, which should be taken into account by breeders when conducting breeding to improve the quality of milk. The highest regression association has been observed between the content of carotene, calcium and phosphorus in blood and milk. If you increase these indicators in the blood by the corresponding unit, their content in milk will also increase by 0,90, 0,31 and 0,42, respectively. The heritability of milk productivity traits in most cases was at medium and low levels and ranged from 0,03 to 0,66 (straight-line correlation method) and from 0,03 to 0,44 (straight-line regression method). The heritability of hematological traits in most cases was at an average level and ranged from 0,14 to 0,98 (straight-line correlation method) and from 0,11 to 0,76 (straight-line regression method). The total protein content in the blood (h² =0,98 и 0,76) and the number of monocytes (h² =0,82 и 0,60) depended more on the genotypic features of the animal. The average level of heritability coeffi cients has been observed in the blood content of total lipids, phosphorus, eosinophils, young neutrophils, and lymphocytes.

PSIV-22 Validation of 26a and let7 microRNA as early pregnancy markers in single cow blood samples, at day 28 after embryo transfer

Abstract In this work we validated the previous scientific reports done in pooled samples by two different groups, over the miRNA expression in single blood from pregnant cows. Particularly we indagate the expression at day 28 after embryo transfer of let 7a, and 26a as well as 16 microRNAs (miRNA) family. By qPCR technique, we demonstrated that let 7a, and 26a increase maximum 2.1 and 2.6 fold change respectively in pregnant vs non-pregnant nulliparous heifers. This represents an exciting opportunity to use the differential expression of these miRNAs as early pregnancy markers and better understand the relationship with the cellular and molecular interaction between mother and embryo.

Expression-based analysis of genes related to single nucleotide polymorphism hits associated with bovine leukemia virus proviral load in Argentinean dairy cattle

In dairy cattle infected with bovine leukemia virus (BLV), the proviral load (PVL) level is directly related to the viral transmission from infected animals to their healthy herdmates. Two contrasting phenotypic groups can be identified when assessing PVL in peripheral blood of infected cows. A large number of reports point to bovine genetic variants (single nucleotide polymorphisms) as one of the key determinants underlying PVL level. However, biological mechanisms driving BLV PVL profiles and infection progression in cattle have not yet been elucidated. In this study, we evaluated whether a set of candidate genes affecting BLV PVL level according to whole genome association studies are differentially expressed in peripheral blood mononuclear cells derived from phenotypically contrasting groups of BLV-infected cows. During a 10-mo-long sampling scheme, 129 Holstein cows were phenotyped measuring anti-BLV antibody levels, PVL quantification, and white blood cell subpopulation counts. Finally, the expression of 8 genes (BOLA-DRB3, PRRC2A, ABT1, TNF, BAG6, BOLA-A, LY6G5B, and IER3) located within the bovine major histocompatibility complex region harboring whole genome association SNP hits was evaluated in 2 phenotypic groups: high PVL (n = 7) and low PVL (n = 8). The log2 initial fluorescence value (N0) transformed mean expression values for the ABT1 transcription factor were statistically different in high- and low-PVL groups, showing a higher expression of the ABT1 gene in low-PVL cows. The PRRC2A and IER3 genes had a significant positive (correlation coefficient = 0.61) and negative (correlation coefficient = -0.45) correlation with the lymphocyte counts, respectively. Additionally, the relationships between gene expression values and lymphocyte counts were modeled using linear regressions. Lymphocyte levels in infected cows were better explained (coefficient of determination = 0.56) when fitted a multiple linear regression model using both PRRC2A and IER3 expression values as independent variables. The present study showed evidence of differential gene expression between contrasting BLV infection phenotypes. These genes have not been previously related to BLV pathobiology. This valuable information represents a step forward in understanding the BLV biology and the immune response of naturally infected cows under a commercial milk production system. Efforts to elucidate biological mechanisms leading to BLV infection progression in cows are valuable for BLV control programs. Further studies integrating genotypic data, global transcriptome analysis, and BLV progression phenotypes are needed to better understand the BLV-host interaction.

Th17-related mammary immunity, but not a high systemic Th1 immune response is associated with protection against E. coli mastitis

Vaccination against bovine mastitis lags behind despite high demand from the dairy industry and margin for efficacy improvement. We previously compared two immunization protocols against E. coli using either only the intramuscular route or a combination of intramuscular and mammary ductal routes, also known as ‘prime and pull’ strategy. A homologous mammary challenge during the memory phase showed that immunization favorably modified the mastitis course, notably in locally immunized cows in comparison to intramuscular and control adjuvant-only groups. Here, we performed whole-blood profiling through RNA-seq transcriptome and plasma cytokine 15-plex analyses at time points of the E. coli mastitis that showed significant clinical and laboratory differences among the groups. Diminished production of inflammatory cytokines and increased IFNγ were detected in the blood of immunized cows, where a T lymphocyte activation profile was evidenced at 12-h post infection. Acute phase neutropenia was less severe in these cows, and pathways related to neutrophil diapedesis and monocyte activation were also present. Furthermore, three intramammary-immunized cows showing faster healing and shorter mastitis duration had gene profiles that differed from their counterparts, but without any clue for the mastitis susceptibility difference. Inasmuch, when gene expression of CD4 T cells was assessed in mammary tissue, enrichment of IL-17-associated pathways was identified in the quarters of intramammary-immunized cows not only after challenge but also in the control quarters that were not infected. These findings indicate that local immunization mobilizes protective mechanisms that rely on the settlement of type 3 immunity-related CD4 T cells prior to infection.

Reproductive recovery in cows with subclinical ketosis

The development of ketosis in high productive cows is accompanied by damage to various organs and systems of the body.Therefore, even with successful treatment, animals have long-term consequences leading to a decrease in milk production, metritis, steroidogenesis disorders, impaired development of follicles and yellow bodies, and infertility. It is because the problem remains poorly understood, which is associated with the special features of the restoration of reproductive function in cows with ketosis. Our research was carried out on 208 cows of Holstein and Ukrainian black-and-white dairy breeds at the age of 2–6 years, with various fatness and productivity of 6–10 thousand kg of milk per lactation. The concentration of β-ketones was determined on the 5th and 10th days after delivery using a CareSens Dual ketometer. After detecting 1.1 and more mmol/l ketone bodiesin the blood of cows, the animal was considered sick and treatment was started according to the farm scheme. After identifying the stage of the reproductive cycle, insemination was carried out using payets. The pregnancy was diagnosed with an ultrasonic device Kaixin KX 5200 on the 35-40 day after insemination. After the research, it was found that on the fifth day after giving birth, an increased content of β-ketones in the blood was found in 42,8%, and on the tenth day – in 21,4% of cows. Of these, in 18,3% of animals the disease was re-detectedon the tenth day after calving. The emergence of subclinical ketosis after parturition is accompanied by decrease(3,6days)of the period from calving to recovery of reproductive cyclicity and the number of pregnant animals(32,7%, p <0.001), and by increase of 17,6 (p <0.001) days in the duration of infertility and insemination index(by 1,3). Re-development of latent ketosis in cows complicated the course of the disease. After a diseaserelapse there was a decrease by 15,1 days (p <0.001) in the impedance period and by 66,7% (p <0.001) of fertility and an increase by 29,4 days(p <0.001) in the service period and by 11,7 the number of sperm doses used for one successful insemination. Key words: ketosis, cow, fertility, insemination index, ketometer, β-ketones, blood, reproductive cycle.

DAIRY PRODUCTIVITY OF UKRAINIAN BLACK-AND-WHITE DAIRY BREED COWS DEPENDING ON THE GENOTYPE

DAIRY PRODUCTIVITY OF UKRAINIAN BLACK-AND-WHITE DAIRY BREED COWS DEPENDING ON THE GENOTYPE

Effect of feeding with processed by-products of forests biomass on metabolism of cows

The effect of aqueous extract of pine needles, pine nut shells and arabinogalactan on the metabolism of dairy cows was studied. The objectives of the research were to investigate the elemental composition of aqueous extract of pine needles, pine nut shells and arabinogalactan and to study the effect of cows’ feeding on aqueous extract containing these supplements on the biochemical parameters of blood, physical and biochemical parameters of cows’ urine. The experiment was carried out in theKrasnoyarskTerritoryon black-and-white cows at the age of fi calving with an average body condition and life weight of 580-600 kg. Two groups of cows were formed, 10 animals in each. The duration of the experiment was 100 days. The cows were kept tied, in year-round stalls, milking was carried out automatically into the milk pipe. According to the scheme of the experiment, the control group was fed on the main diet, the experimental group was given aqueous extract (200 ml/head/day) of pine needles (15 g/l), pine nut shells (25 g/l) and arabinogalactan (25 g/l) in addition to the main diet. The test infusion was fed in a mixture with concentrated feed. The feeding ration corresponded to the zootechnical standards. It was found that feeding cows on aqueous extract of the studied forest components did not have a negative eff on the metabolism in the body of the animals: the biochemical parameters of blood and the physicochemical parameters of cows’ urine corresponded to the norm of healthy animals. At the same time, metabolic processes in the blood of the cows in the experimental group were very intensive, mineral and carbohydrate metabolism improved, as evidenced by an increase in glucose content by 16.6%, iron – by 17.8, phosphorus – by 13.0%.

Результат использования сенажа из эспарцета песчаного в рационах молочных коров

Abstract. The purpose of the research was to study the effect of feeding by sainfoin haylage in the dry period (21 days before calving) and in the period of early lactation (50 days after calving) on the metabolic processes of dairy cows and the results of reproduction. The article gives the brief description of sainfoin (Onobrychis arenaria Kit.) as a valuable feed protein crop that can be successfully cultivated in Perm region conditions. High sugar content in sainfoin forage was noted 4.86 %, which is 1.6 times higher compared with standard class 1 for legume haylage first class. Methods. Experiments for use of sainfoin haylage in feeding highly productive cows were conducted in 2018. Cows of the experimental groups received a diet including sainfoin haylage. Cows in the control group received 5 kg of poaceous grass hay as a fodder. The first experimental group got 50 % of forage dry matter as sainfoin haylage. The second experimental group received 100 % of forage dry matter as sainfoin haylage. Results. Hay replacing by equal dry matter amount of sainfoin haylage provided positive effect on immuno-biochemical parameters of cows blood. The protein content increased in the blood plasma of cows during the time of the experiment: in the second experimental group by 4.3 g/l (5.47 %, p < 0.01) and reached 82.80 g/l, in the first experimental group by 1.72 % (p < 0.05), in the control – by 2.28 % (p < 0.05). Feeding sainfoin haylage to cows had a positive effect on their reproductive functions. The service period for cows of the second experimental group was shorter by 8.1 days (9.28 %, p < 0.01) compared with the first experimental group and by 21 days (24.05 %, p < 0.05) shorter than in the control group. The scientific originality of the work is that for the first time the biochemical composition of sainfoin was studied thoroughly in Perm region and the results of sainfoin haylage feeding to animals were presented.

The content of total protein and protein fractions in cows during pregnancy and their diagnostic value

The aim of the article was to study the content of total protein and protein fractions in the blood of cows during pregnancy and determine their diagnostic value. The studies were performed as follows. Within 7 days after insemination on the principle of analogues selected a group of 100 cows. Pregnancy was diagnosed by rectal examination of cows 60 days after insemination. In experimental animals, starting from the 25th to the 29th day after insemination, blood samples for morphological and biochemical studies were taken monthly before delivery. The total protein content in the serum was determined by the biuret reaction, protein fractions – nephelometric method. Depending on the course of labor and the postpartum period, cows were divided into two groups. The first group included 30 animals with a physiological course of the postpartum period. The second group was formed of 30 cows with subinvolution of the uterus. According to this goal, the dynamics of total protein, albumin, alpha-, beta- and gammaglobulins in the blood of cows with physiological course of pregnancy and prone to the development of subinvolution of the uterus from the first to 9 months of pregnancy. It was found that in clinically healthy cows the onset of pregnancy is characterized by a high (0.89) albumin-globulin ratio, which in the fourth month of pregnancy decreases to 0.78, in the fifth or sixth months increases to 0.85, followed by a decrease to 0.78 before childbirth. In cows prone to uterine subinvolution, a significant difference in albumin content was found in the first month of pregnancy (47.07 ± 1.21 vs. 41.02 ± 1.26 %). In the third to fifth months of pregnancy, there was an increase in the proportion of albumin in the protein to 44.62 ± 0.88 % and a new decrease in the seventh to ninth months to 41.96 ± 0.3 %. In the first month of pregnancy, the albumin-globulin ratio was 0.7, with a tendency to increase in the fifth to sixth months of pregnancy to 0.8, and decrease to 0.72 before calving. Analyzing the dynamics of alpha-globulins in clinically healthy cows, we noted two peaks of decrease in their percentage - in the third month of pregnancy (from 16.02 ± 0.08 to 15.14 ± 0.06 %) and in the fifth month , 96 ± 0.48 %) and a consistent increase in the seventh to ninth months of pregnancy (up to 17.22 ± 0.13 %). And in cows prone to the development of uterine subinvolution, with a slightly higher percentage at the beginning of pregnancy (16.02 ± 0.08 to 17.08 ± 0.05 %), we did not notice significant changes in the percentage of this globulin, except for a significant increase in seventh to ninth month of pregnancy (up to 18.01 ± 0.24 – 19.01 ± 0.32 %). It is proposed to use the albumin-globulin ratio at 6, 7, 8, 9 months of gestation as a prognostic factor in the detection of cows prone to uterine subinvolution.

СРАВНИТЕЛЬНАЯ ОЦЕНКА КОСТРОМСКОЙ И ЯРОСЛАВСКОЙ ПОРОД КРУПНОГО РОГАТОГО СКОТА ПО ГЕНУ БЕТА-КАЗЕИНА

Abstract. The article presents the results of a comparative assessment of the frequency of alleles and genotypes for the gene of beta-casein (β-CN) in cattle of the Kostroma and Yaroslavl breeds. The data on 137 bulls-producers of comparable and related breeds, presented on the official sites of “OOO Head Center for the Reproduction of Farm Animals” and the breeding farm “OOO Yaroslavskoe”, have been analyzed. The blood of cows from the Kostroma and Yaroslavl breeds in the breeding farms of the Kostroma region served as the object of DNA research. A total of 80 animals were genotyped. Evaluation of the distribution of the desired genotype β-CNА2/А2 in stud bulls showed that two breeds are in the lead – the Schwyz and the Kostroma breed, with the frequency of occurrence – 0.9090 and 0.6250, respectively. Moreover, in the Kostroma breed, there are no bulls carriers of the homozygous β-CNА1/А1 genotype. If we take into account the frequency of distribution of the β-CNА2 allele in bulls of the analyzed breeds, then it is higher – in the Schwyz breed – 0.9091, Kostroma – 0.8125 and Holstein – 0.6776. In bulls of the Yaroslavl breed there is 0.2083 frequency of the β-CNА2/А2 genotype and 0.4167 frequency of the β-CNА2 allele. A comparative assessment of the polymorphism data of the beta-casein locus in cows of the Yaroslavl and Kostroma breeds showed that in the Yaroslavl breed there were least of all animals with the β-CNА2/А2 genotype – 0.0333 and more – with the β-CNА1/А1 genotype – 0.6667. In the Kostroma breed, the genetic situation is completely different. So cows with genotypes β-CNА2/А2 – 0.5490, and with genotype β-CNА1/А1 – 0.0785. However, it is necessary to take into account the main indicators of cattle productivity in order to consider the beta-casein genotype as an additional selection trait. It is not possible to study the milk productivity of Yaroslavl breed cows with different genotypes, since only one cow with the CNА2/А2 genotype has been identified. However, cows of the Kostroma breed homozygous for the CNА2 allele, for the third and older lactations, had a milk yield of 1534 kg higher than cows homozygous for the CNА1 allele. Consequently, the beta-casein genotype can be considered an additional promising breeding trait.

Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows Affected by Parietal Fibrinous Peritonitis after Caesarean Section.

The aim of this study was to identify the pathogens potentially involved in parietal fibrinous peritonitis (PFP). PFP is a complication of laparotomy in cattle, characterized by an accumulation of exudate inside a fibrinous capsule. We have studied 72 cases of PFP in Belgian blue cows, confirmed by a standard diagnostic protocol. Blood was collected to evaluate the presence of antibodies for Mycoplasma bovis (M. bovis), Coxiella burnetii (C. burnetii) and Bovine Herpesvirus 4 (BoHV4) by enzyme-linked immunosorbent assays. Peritoneal exudate was obtained from the PFP cavity to perform bacteriological culture, and to identify the DNA of M. bovis, C. burnetii and BoHV4 using real time polymerase chain reaction (qPCR). Bacteriological culture was positive in most peritoneal samples (59/72); Trueperella pyogenes (T. pyogenes) (51/72) and Escherichia coli (E. coli) (20/72) were the most frequently identified. For BoHV4, the majority of cows showed positive serology and qPCR (56/72 and 49/72, respectively). Contrariwise, M. bovis (17/72 and 6/72, respectively) and C. burnetii (15/72 and 6/72, respectively) were less frequently detected (p < 0.0001). Our study proves that PFP can no longer be qualified as a sterile inflammation. Moreover, we herein describe the first identification of BoHV4 and C. burnetii in cows affected by PFP.

Detection of latent forms of Mycobacterium avium subsp. paratuberculosis infection using host biomarker-based ELISAs greatly improves paratuberculosis diagnostic sensitivity.

Bovine paratuberculosis (PTB) is a chronic granulomatous enteritis, caused by Mycobacterium avium subsp. paratuberculosis (MAP), responsible for important economic losses in the dairy industry. Current diagnostic methods have low sensitivities for detection of latent forms of MAP infection, defined by focal granulomatous lesions and scarce humoral response or MAP presence. In contrast, patent infections correspond to multifocal and diffuse types of enteritis where there is increased antibody production, and substantial mycobacterial load. Our previous RNA-Seq analysis allowed the selection of five candidate biomarkers overexpressed in peripheral blood of MAP infected Holstein cows with focal (ABCA13 and MMP8) and diffuse (FAM84A, SPARC and DES) lesions vs. control animals with no detectable PTB-associated lesions in intestine and regional lymph nodes. The aim of the current study was to assess the PTB diagnostic potential of commercial ELISAs designed for the specific detection of these biomarkers. The ability of these ELISAs to identify animals with latent and/or patent forms of MAP infection was investigated using serum from naturally infected cattle (n = 88) and non-infected control animals (n = 67). ROC analysis revealed that the ABCA13-based ELISA showed the highest diagnostic accuracy for the detection of infected animals with focal lesions (AUC 0.837, sensitivity 79.25% and specificity 88.06%) and with any type of histological lesion (AUC 0.793, sensitivity 69.41% and specificity 86.57%) improving on the diagnostic performance of the popular IDEXX ELISA and other conventional diagnostic methods. SPARC and MMP8 showed the highest diagnostic accuracy for the detection of animals with multifocal (AUC 0.852) and diffuse lesions (AUC 0.831), respectively. In conclusion, our results suggest that quantification of ABCA13, SPARC and MMP8 by ELISA has the potential for implementation as a diagnostic tool to reliably identify MAP infection, greatly improving early detection of MAP latent infections when antibody responses and fecal shedding are undetectable using conventional diagnostic methods.

Age dynamics of hematological indices in dry cows

The requirement for the study of blood parameters is determined by its physiological significance and changes that occur in different physiological and pathological conditions. The hematological profile is an important criterion that shows the physiological condition of animals. The physiological changes of blood in cows have been established concerning the conditions of keeping and feeding, hygienic parameters, in the dynamics of the dry season, in different seasons of the year, etcetera. We have not found any information on studies of the dynamics of blood parameters in pregnant animals, depending on their age, in the study of literary sources in recent years. The purpose of our work was to study the age-related dynamics of hematological blood parameters in cows during the dry period. Our studies aimed to determine the hematological indices of Holstein breed cows in the physiological course of the prenatal period during the dry period, depending on the number of deep calving. Control and experimental groups were formed on the principle of group-analogues (by breed, age, sex, physiological state, weight, milk productivity). Animals kept in typical rooms with satisfactory microclimate parameters (in winter) and at camps (in summer). Laboratory studies were carried out in the laboratory of the Dnipro State Agrarian and Economic University. Hematologic studies were performed according to the following indicators: erythrocytes, hemoglobin, color index, erythrocyte sedimentation rate, leukocytes, monocytes, lymphocytes, neutrophils with rod-like shape and segmented nucleus, basophils and eosinophils according to conventional methods. The results of our studies indicate that the lowest number of erythrocytes was detected in the cows of the sixth calving and the highest hemoglobin content, which led to the rise of the color index – in cows of the fifth pregnancy present the reverse tendency; increase to the maximum values of the number of leukocytes in cows of the third body (1.32 times), which is a reflection of the greater reactivity of the body of cows of this age group; a probable decrease (by 10.59 %, P &lt; 0.05) of lymphocyte counts was found in sixth-calf cows, indicating a weakened immune response; also increased eosinophils almost 1.75 times (P &lt; 0.05) compared to fifth pregnancy; the trend of a gradual decrease in the number of segmented neutrophils in calving cows is followed by a second to fifth pregnancy and a significant reduction in the number of stab neutrophils in cows of the fifth pregnancy of up to 1.33 %.

Influence of farm conditions on sows’ morphological blood indicators

The article summarizes the influence of farm conditions on sows blood morphological parameters. Our research was carried out in the farms of Zolochiv and Starosambir districts of the Lviv region (Ukraine) and revealed strong violations of animals feeding, both in terms of nutrition, and on the macro- and microelement composition. In all the farms, there was practically no appropriate way to feeding sows from different physiological groups, which may further adversely affect the growth and development of the resulting offspring. The parameters of the microclimate in the premises were invested in the limits of permissible norms. The differences between farms were production process and pigs keeping. Animals of the Zolochiv region were not registered and had the opportunity to move freely, to nest before the farrowing, to satisfy their physiological needs for food search (to dig, to throw the bedding), without barriers to consume food and water. In the farm of the Starosambir district the sows were kept in individual fixing machines, they were restricted in movement, free choice of place for rest, manifestation of natural behavior, the ability to satisfy the instinct of nesting and show the instinct of caring for the offspring. Free holding of sows have a positive effect on the processes of hematopoiesis. The blood of these animals had more red blood cells and the hemoglobin concentration was higher. It was also proved that the most susceptible to the conditions of containment were farrowing sows from both farms, as evidenced by the morphological indices of blood. Thus, the number of erythrocytes, hemoglobin concentration, hematocrit magnitude and red blood indices in animals of this physiological group are recorded at the lower limit of the physiological norm.

Physiological characteristics of cattle of different ages

A major role in the normal course of ontogenesis of agricultural animals play age-related changes is highly variable hematological parameters. Even in one household from healthy cattle they can have very wide variations. Especially great practical significance have a total blood protein and its fractions, activity of the system-oxidation and indicators of natural resistance. In the work conducted and the parameters of protein metabolism in cows of three and five years of age were comparable, the globulins in the blood there were a few more, and the albumin was slightly less. Level antiokislitelnoe activity of blood in adult cows with increased age, thus ensuring they have a decrease in the blood between three and five years of life the number of products of peroxidation. In the blood of the examined cows with age increased levels of lysozyme and seromukoidov and decreased the number of circulating immune complexes. In calves between 5 days and 1.5-month age there was increase of total protein and its fractions. This was accompanied by increased antioxidant activity in their blood with a decrease in the level of diene conjugates and malonic dialdehyde. With increasing age of calves lysozyme activity of blood decreased a little in the stability of the concentration of seromukoidov and the increase in the number of circulating immune complexes. The obtained data can be regarded as normative for further research on cattle kept under the conditions of Central Russia.