Coral Fragments Research Articles

Transcriptome characterization of Pocillopora grandis transplanted into reefs with different health conditions: potential stress indicators at the holobiont level

Understanding the potential of coral to adapt to environmental stressors that cause coral bleaching is urgent. The molecular responses of the coral holobiont to such stress conditions determine the success of symbiosis. Therefore, by characterizing molecular stress responses at the holobiont level, we can develop better tools to diagnose its health and resilience. However, only some genomic-scale resources are available for reefbuilding corals from the Eastern Tropical Pacific. This study aimed to perform a transcriptomic characterization of the Pocillopora grandis holobiont following transplantation into environments with different health conditions in Colima, Mexico. Healthy specimens of two color morphotypes (green and brown) were collected in Carrizales, a reef in good condition. Coral fragments from the two morphotypes were relocated within the source location (local transplant stress). In contrast, similar fragments were translocated into another reef with a poorer health state, La Boquita. After 24 h, the transplanted fragments were collected, and RNA-seq was performed with the Illumina system. De novo transcriptome assembly, functional annotation, identification of co-expression modules, and enrichment analysis of molecular pathways were performed. The analysis of rRNA LSU, rRNA SSU, and COI sequences confirmed the coral species, whereas analysis of Rubisco, psbA, and psaA transcripts revealed that the dominant endosymbiont was Durusdinium sp. Gene expression patterns observed across samples suggest that the transplantation to a reef with a poorer state of health affected processes such as photosynthesis, calcium homeostasis, and immune response. The transcriptomic indicators proposed here are valuable for further studies examining the adaptation of Pocillopora corals to global changes.

Stronger Hurricanes and Climate Change in the Caribbean Sea: Threats to the Sustainability of Endangered Coral Species

An increasing sea surface temperature as a result of climate change has led to a higher frequency and strengthening of hurricanes across the northeastern Caribbean in recent decades, with increasing risks of impacts to endangered corals and to the sustainability of coral reefs. Category five Hurricanes Irma and María during 2017 caused unprecedented damage to coral reef ecosystems across northeastern Puerto Rico, including mechanical destruction, localized sediment bedload (horizontal sediment transport and abrasion), and burial by hurricane-generated rubble fields. Hurricanes inflicted significant site-, depth-, and life history trait-specific impacts to endangered corals, with substantial and widespread mechanical damage to branching species, moderate mechanical damage to foliose species, and moderate to high localized damage to small-sized encrusting and massive morphotypes due to sediment bedload and burial by rubble. There was a mean 35% decline in Acropora palmata live cover, 79% in A. cervicornis, 12% in Orbicella annularis, 7% in O. faveolata, 12% in O. franksi, and 96% in Dendrogyra cylindrus. Hurricane disturbances resulted in a major regime shift favoring dominance by macroalgae, algal turf, and cyanobacteria. Recovery from coral recruitment or fragment reattachment in A. palmata was significantly higher on more distant coral reefs, but there was none for massive endangered species. Stronger hurricanes under projected climate change may represent a major threat to the conservation of endangered coral species and reef sustainability which will require enhancing coral propagation and restoration strategies, and the integration of adaptive, ecosystem-based management approaches. Recommendations are discussed to enhance redundancy, rapid restoration responses, and conservation-oriented strategies.

Flexi-chambers and 3D photogrammetry effectively measure coral fragment physiology in situ

Non-invasive techniques for measuring the physiological performance of corals in situ are important for understanding responses to changing environmental conditions. Two such methods are the ‘flexi-chamber’ and three-dimensional (3D) photogrammetry. While these have been verified for coral colonies, they have not been tested on coral fragments which are commonly used in field experiments. This study aimed to determine if these methods can effectively measure respiration, photosynthesis, and growth of fragments of massive and sub-massive coral species in the intertidal and subtidal habitats in Park Rynie, South Africa (30.3167° S, 30.7333° E). Differences in respiration and photosynthesis rates between species and habitat were successfully measured using the flexi-chambers. The coral fragments did not show any visual signs of stress (excess mucus production or loss of pigmentation) during the 3-h incubations or an hour after the incubations. 3D models were successfully constructed for all fragments. This study showed that flexi-chambers and 3D photogrammetry can be effectively used to measure the respiration, photosynthesis, and growth of individual coral fragments (3 cm > 4 cm) of different morphology and habitats. The combination of the two methods permits measures of fundamental physiological processes to be attained at relatively low cost and low complexity. This will facilitate studies of a wide range of coral species in situ generating more knowledge on responses to environmental variability.

Impacts of plastic-free materials on coral-associated bacterial communities during reef restoration.

Coral propagation and out-planting based restoration approaches are increasingly being applied to assist natural recovery of coral reefs. However, many restoration methods rely on plastic zip-ties to secure coral material which is potentially problematic for the marine environment. Plastic-free biodegradable alternatives may however pose unique risks to coral-associated bacterial communities integral to coral health. Therefore, to identify whether biodegradable materials differentially impact coral-associated bacterial communities we examined Acropora millepora coral-associated bacterial communities during propagation in two experiments on the Great Barrier Reef. Coral fragments were secured to coral nurseries with conventional plastic, metal, or biodegradable (polyester and polycaprolactone) ties. Tie failure and coral-associated bacterial communities were then characterized over six months. Minimal coral mortality was observed (3.6%-8%) and all ties had low failure rates (0%-4.2%) except for biodegradable polyester ties (29.2% failure). No differences were observed between coral-associated bacterial communities of fragments secured with different ties, and no proliferation of putatively pathogenic bacteria was recorded. Overall, our findings suggest that reducing reliance on conventional plastic is feasible through transitions to biodegradable materials, without any notable impacts on coral-associated bacterial communities. However, we caution the need to examine more coral taxa of different morphologies and new plastic-free materials prior to application.

Escaping the benthos with Coral Reef Arks: effects on coral translocation and fish biomass.

Anthropogenic stressors like overfishing, land based runoff, and increasing temperatures cause the degradation of coral reefs, leading to the loss of corals and other calcifiers, increases in competitive fleshy algae, and increases in microbial pathogen abundance and hypoxia. To test the hypothesis that corals would be healthier by moving them off the benthos, a common garden experiment was conducted in which corals were translocated to midwater geodesic spheres (hereafter called Coral Reef Arks or Arks). Coral fragments translocated to the Arks survived significantly longer than equivalent coral fragments translocated to Control sites (i.e., benthos at the same depth). Over time, average living coral surface area and volume were higher on the Arks than the Control sites. The abundance and biomass of fish were also generally higher on the Arks compared to the Control sites, with more piscivorous fish on the Arks. The addition of Autonomous Reef Monitoring Structures (ARMS), which served as habitat for sessile and motile reef-associated organisms, also generally significantly increased fish associated with the Arks. Overall, the Arks increased translocated coral survivorship and growth, and exhibited knock-on effects such as higher fish abundance.

Evaluation of coral growth and survival on spider frame transplant media on Samalona Island, Makassar

Coral reefs are one of the main ecosystems in coastal areas that are very stretched with damage, so it requires efforts to preserve them through rehabilitation activities. This study aims to determine the growth rate and survival of transplanted corals from 2021 - 2023 on Samalona Island, Makassar City. This study uses a survey method approach by making direct observations and measurements of transplanted corals on spider frames by utilizing previous data (2021) and comparing current measurement data (2023). The type of transplanted coral studied was Acropora branching at a depth of 3-4 meters. The results showed that the growth rate of transplanted corals was 9.22 cm with an average per month of 0.38 cm. Its survival was 58.15% with the number of surviving coral fragments as many as 314 and dead as many as 226.

Acute Toxicity Assays with Adult Coral Fragments: A Method for Standardization.

Coral reefs are globally declining due to various anthropogenic stressors. Amongst those, chemical pollutants, such as pesticides from agricultural runoff, sewage or an overabundance of personal care products in coastal waters due to intense tourism, may be considered as a local stressor for reef-building corals. The extent to which such chemicals exhibit toxic effects towards corals at environmentally relevant concentrations is currently controversially discussed and existing studies are often based on varying and sometimes deficient test methods. To address this uncertainty, we adapted available methods into a reliable and comprehensive acute coral toxicity test method for the reef-building coral Montipora digitata. The toxicities of the four substances benzophenone-3 (BP-3), Diuron (DCMU), copper (Cu2+ as CuCl2, positive control) and dimethylformamide (DMF, solvent) were assessed in a 96 h semi-static test design. Endpoints such as maximum quantum yield, bleaching, tissue loss and mortality were evaluated with respect to their suitability for regulatory purposes. Overall, the endpoints bleaching and mortality yielded sensitive and robust results for the four tested substances. As the test method follows the principles of internationally standardized testing methods (ISO, OECD), it can be considered suitable for further validation and standardization. Once validated, a standardized test method will help to obtain reproducible toxicity results useful for marine hazard and risk assessment and regulatory decision making.

The Growth Rate of Cyphastrea Coral Fragment Under Controlled Condition

The role of corals was very important in coastal and marine ecosystems, whether in terms of education, ecology, and socio-economy. Coral growth can be used as an indicators of marine ecosystem health. This study was aimed to observed survival rate and the growth rate of Cyphastrea coral fragments. Cyphastrea coral fragments growth rate was observed in ex-situ sites and was conducted over a period of 3 months. The micro-fragmentation method was applied in this study. Several factors such as water temperature, light levels, and nutrient concentrations were measured and controlled throughout the study. The growth rate of Cyphastrea coral fragments was measured using ImageJ software. Result of this study exhibited that survival rate of Cyphastrea was 100% and the growth rate of Cyphastrea was 0.012 cm2/month. This study provides important information on the factors that affect the growth rate of Cyphastrea coral fragments in ex-situ sites. The result of this study can be used to develop more effective strategies for coral reef restoration.

Selectively Positioned Catechol Moiety Supports Ultrashort Self-Assembling Peptide Hydrogel Adhesion for Coral Restoration.

Coral reef survival is threatened globally. One way to restore this delicate ecosystem is to enhance coral growth by the controlled propagation of coral fragments. To be sustainable, this technique requires the use of biocompatible underwater adhesives. Hydrogels based on rationally designed ultrashort self-assembling peptides (USP) are of great interest for various biological and environmental applications, due to their biocompatibility and tunable mechanical properties. Implementing superior adhesion properties to the USP hydrogel compounds is crucial in both water and high ionic strength solutions and is relevant in medical and marine environmental applications such as coral regeneration. Some marine animals secrete large quantities of the aminoacids dopa and lysine to enhance their adhesion to wet surfaces. Therefore, the addition of catechol moieties to the USP sequence containing lysine (IIZK) should improve the adhesive properties of USP hydrogels. However, it is challenging to place the catechol moiety (Do) within the USP sequence at an optimal position without compromising the hydrogel self-assembly process and mechanical properties. Here, we demonstrate that, among three USP hydrogels, DoIIZK is the least adhesive and that the adhesiveness of the IIZDoK hydrogel is compromised by its poor mechanical properties. The best adhesion outcome was achieved using the IIZKDo hydrogel, the only one to show equally sound adhesive and mechanical properties. A mechanistic understanding of this outcome is presented here. This property was confirmed by the successful gluing of coral fragments by means of IIZKDo hydrogel that are still thriving after more than three years since the deployment. The validated biocompatibility of this underwater hydrogel glue suggests that it could be advantageously implemented for other applications, such as surgical interventions.

Effect of fragment size on growth and survival rate of Sarcophyton sp.

The Sarcophyton sp. are very strong and dominant in many coral reef areas. Sarcophyton species are characterized by a distinct sterile stalk, a broad, flared, smooth, mushroom-shaped top, with a wide distribution and dominance in numerous coral reef areas extending from the Red Sea and eastern Africa to the western Pacific Ocean. They are cherished in marine aquariums for their diverse colors and adaptability. However, their excessive exploitation has significantly impacted resources and disrupted the balance of the soft coral biome that inhabits coral reefs. The objective of the current study was to assess the impact of fragment size (0.5 × 0.5; 1.0 × 1.0; 1.5 × 1.5; 2.0 × 2.0, and 2.5 × 2.5 cm) on survival rate, growth rate and time of attachment of coral Sarcophyton sp. in a closed seawater system. Each treatment involved three replicates with 20 cuttings per replicate, and the experimental period was 90 days. The experiment showed that the fragment size did not influence the growth rate of oral disc diameter, pedal disk diameter, and the time attachment of Sarcophyton sp. However, the size of the fragment affects the survival rate, height growth rate, and weight of Sarcophyton sp. colonies. These findings collectively suggest that the initial coral fragment size of 1.5 cm was suitable in laboratory conditions.

Coral trophic pathways impact the allocation of carbon and nitrogen for egg development after bleaching

The persistence of coral reefs requires the survival of adult coral colonies and their continued sexual reproduction despite thermal stress. To assess the trophic pathway (i.e., autotrophy and/or heterotrophy) used to develop gametes following bleaching, we thermally stressed Montipora capitata for one month at a time when corals in Hawai’i typically experience elevated seawater temperatures. After six and nine months of recovery, we pulse-chased non-bleached and previously bleached colonies using a dual-label design to compare the allocation of carbon and nitrogen at significant stages of gamete development. Dissolved inorganic carbon- (DI13C) and nitrogen- (DI15N) labelled seawater or 13C- and 15N-labelled rotifers were used to assess the autotrophic and heterotrophic pathways, respectively. At multiple time points for up to two years later, we collected adult coral fragments and isolated host tissue, Symbiodiniaceae cells, and developing eggs and captured gamete bundles to analyze their carbon (δ13C) and nitrogen (δ15N) stable isotopes. We found that the presence of Symbiodiniaceae was important for gametogenesis in both non-bleached and previously bleached colonies in two main ways. First, autotrophically-acquired carbon and nitrogen were both allocated to gametes during development, suggesting that recovery of photosynthesis after bleaching is critical for gametogenesis. Second, only heterotrophically-acquired nitrogen, not carbon, was incorporated into gametes and was readily recycled between host tissues and Symbiodiniaceae cells. This suggests that one of the purposes of heterotrophy following coral bleaching for M. capitata may be to supplement the nitrogen pool, providing available nutrients for endosymbiotic algal growth. Allocation of carbon and nitrogen to eggs coincided with the period when vertical transmission of symbionts to gametes occurs, further supporting the important relationship between gametogenesis and availability of Symbiodiniaceae for M. capitata.

Video Transect-Based Coral Demographic Investigation

Demographic studies that quantify species’ performances for survival, growth, and reproduction are powerful means to characterize sources of demographic bottlenecks and predict community dynamics. However, they require fine-scale surveys of populations in the field, and are often too effort-intensive to be replicable at a large scale and in the long term. We developed a standardized digital approach for extracting demographic data on species’ abundances, sizes, and positions within video transects, enabling back-from-the-field data acquisition and therefore optimizing time spent in the field. The approach is based on manual species identification, size measurements, and mapping in video transects, mimicking what is traditionally performed in the field, though it can be automated in the future with the deployment of artificial intelligence. We illustrate our approach using video surveys of a reef-building coral community in New Caledonia. The results characterize the composition of the coral community and demographic performances as key ecological indicators of coral reef health, shed light on species’ life strategies and constraints to their demographics, and open paths for further quantitative investigations. Key findings include the diversity of life strategies with contrasting levels of investment in survival, growth, and reproduction found among the six taxa dominating the coral community (Acropora, Montipora, Porites, Galaxea, Favia, Millepora), indicating the diversity of demographic paths to ecological success. Our results also indicate that several species have adapted mechanisms to prevail under limiting hydrodynamic environments through the propagation of coral fragments. Our approach facilitates image-based demographic investigations, supporting endeavors in ecology and ecosystem management.

The success of coral Acropora sp. transplantation with various methods in Raja Ampat Regency, Indonesia

Climate change and anthropogenic pressures have caused massive damage and degradation of coral reef ecosystem services in almost all countries in the Indian Ocean. The status of coral reefs at 1,067 sites in Indonesia is included in the poor category with 386 sites (36.18%) and the reefs in the moderate category with 366 sites (34.3%). The condition, area, and function of this coral reef ecosystem can be restored through rehabilitation activities using the transplant method. This study aimed to determine the success of coral Acropora sp transplantation in two different methods in Raja Ampat Regency, i.e., Yensawai Village. The research was conducted from September to December 2021 using PVC and iron racks. The increase in length of coral fragments was measured using a caliper and survival was calculated for the number of coral fragments at the end of the study. The results showed that coral fragments with PVC racks had a growth rate of 0.67±0.54 mm/day and a survival rate of 81.25%, while those on iron racks had a growth rate of 0.64±0,66 mm/day and a survival rate of 90%. Both methods showed a high coral survival rate (>80%). T-test statistical analysis revealed no apparent variance between the two approaches (p= 0.85). Our findings suggest that both methods are suitable for use in Yensawai Village.

Heat-evolved algal symbionts enhance bleaching tolerance of adult corals without trade-off against growth.

Ocean warming has caused coral mass bleaching and mortality worldwide and the persistence of symbiotic reef-building corals requires rapid acclimation or adaptation. Experimental evolution of the coral's microalgal symbionts followed by their introduction into coral is one potential method to enhance coral thermotolerance. Heat-evolved microalgal symbionts of the generalist species, Cladocopium proliferum (strain SS8), were exposed to elevated temperature (31°C) for ~10 years, and were introduced into four genotypes of chemically bleached adult fragments of the scleractinian coral, Galaxea fascicularis. Two of the four coral genotypes acquired SS8. The new symbionts persisted for the 5 months of the experiment and enhanced adult coral thermotolerance, compared with corals that were inoculated with the wild-type C. proliferum strain. Thermotolerance of SS8-corals was similar to that of coral fragments from the same colony hosting the homologous symbiont, Durusdinium sp., which is naturally heat tolerant. However, SS8-coral fragments exhibited faster growth and recovered cell density and photochemical efficiency more quickly following chemical bleaching and inoculation under ambient temperature relative to Durusdinium-corals. Mass spectrometry imaging suggests that algal pigments involved in photobiology and oxidative stress were the greatest contributors to the thermotolerance differences between coral hosting heat-evolved versus wild-type C. proliferum. These pigments may have increased photoprotection in the heat-evolved symbionts. This is the first laboratory study to show that thermotolerance of adult corals (G. fascicularis) can be enhanced via the uptake of exogenously supplied, heat-evolved symbionts, without a trade-off against growth under ambient temperature. Importantly, heat-evolved C. proliferum remained in the corals in moderate abundance 2 years after first inoculation, suggesting long-term stability of this novel symbiosis and potential long-term benefits to coral thermotolerance.

Evidence of Sexual Reproduction in Out-Planted Coral Colonies

Intervention techniques to restore coral communities have become an important management tool to help recover and rehabilitate damaged reefs. The direct transplantation of healthy coral fragments is the most common method; however, there is controversy in the long-term success, as using coral clones may diminish the genetic diversity of the coral population. Genetic recombination can be achieved when the coral colony produces gametes and eventually reproduces; therefore, it is important to provide evidence that restored colonies produce gametes as their naturally recruited counterparts with similar colony size (age). Natural and restored Pocillopora coral colonies of the same size range (between 40 and 50 cm in diameter) were tagged and sampled during the rainy season to determine gamete maturation. Our results show no differences in the reproductive activity among colonies: natural and restored coral colonies matured gametes from June to October, with a peak in sexually active coral colonies in July. Also, gamete malformation was not observed. During the gamete production period, the area’s temperature ranged from 27.9 to 30.02 °C. The results’ evidence that coral colonies formed through active restoration contribute not only to the increase in live coral cover as seen in previous studies but potentially contribute in the medium term (>5 years after out-planting) to the production of larvae and local and subsidiary recruitment, since they exhibit the same reproductive patterns as their naturally formed counterparts and no differences in the reproductive activity among coral colonies. Therefore, long-term coral restoration projects contribute to maintaining the live coral cover and the genetic diversity in the region, eventually rehabilitating the coral community.

Cellular traits regulate fluorescence-based light-response phenotypes of coral photosymbionts living in-hospite.

Diversity across algal family Symbiodiniaceae contributes to the environmental resilience of certain coral species. Chlorophyll-a fluorescence measurements are frequently used to determine symbiont health and resilience, but more work is needed to refine these tools and establish how they relate to underlying cellular traits. We examined trait diversity in symbionts from the generas Cladocopium and Durusdinium, collected from 12 aquacultured coral species. Photophysiological metrics (ΦPSII, σPSII, ρ, τ1, τ2, antenna bed quenching, non-photochemical quenching, and qP) were assessed using a prototype multi-spectral fluorometer over a variable light protocol which yielded a total of 1,360 individual metrics. Photophysiological metrics were then used to establish four unique light-response phenotypic variants. Corals harboring C15 were predominantly found within a single light-response phenotype which clustered separately from all other coral fragments. The majority of Durusdinium dominated colonies also formed a separate light-response phenotype which it shared with a few C1 dominated corals. C15 and D1 symbionts appear to differ in which mechanisms they use to dissipate excess light energy. Spectrally dependent variability is also observed across light-response phenotypes that may relate to differences in photopigment utilization. Symbiont cell biochemical and structural traits (atomic C:N:P, cell size, chlorophyll-a, neutral lipid content) was also assessed within each sample and differ across light-response phenotypes, linking photophysiological metrics with underlying primary cellular traits. Strong correlations between first- and second-order traits, such as Quantum Yield and cellular N:P content, or light dissipation pathways (qP and NPQ) and C:P underline differences across symbiont types and may also provide a means for using fluorescence-based metrics as biomarkers for certain primary-cellular traits.

Spatial extent of dysbiosis in the branching coral Pocillopora damicornis during an acute disease outbreak

Globally, coral reefs face increasing disease prevalence and large-scale outbreak events. These outbreaks offer insights into microbial and functional patterns of coral disease, including early indicators of disease that may be present in visually-healthy tissues. Outbreak events also allow investigation of how reef-building corals, typically colonial organisms, respond to disease. We studied Pocillopora damicornis during an acute tissue loss disease outbreak on Guam to determine whether dysbiosis was present in visually-healthy tissues ahead of advancing disease lesions. These data reveal that coral fragments with visual evidence of disease are expectedly dysbiotic with high microbial and metabolomic variability. However, visually-healthy tissues from the same colonies lacked dysbiosis, suggesting disease containment near the affected area. These results challenge the idea of using broad dysbiosis as a pre-visual disease indicator and prompt reevaluation of disease assessment in colonial organisms such as reef-building corals.

Shallow Coastal Carbonate Sediment Mapping for Biomimetics MICP, Case Study of Indonesia Beachrock, Yogyakarta

The nomenclature for beachrock sedimentary has been in scientific discussions prior to the notable methodology of sustainable cementitious binding material based on microbially induced calcium carbonate (MICP). Typically, coastal erosion mitigation involves modifying surface conditions using materials like concrete, among other materials. This study aims to challenge the conventional practice by evaluating the feasibility of expeditiously constructing artificial beachrock utilizing natural components (e.g., microbes, sand, shells, coral fragments, seaweed, etc.) and propose it as an innovative coastal protection strategy. The primary focus of this research is to characterize the Java South coast, specifically Parangtritis Beach and Krakal-Sadranan Beach, where beachrock formations are present. Subsequent investigations were conducted to (i) identify occurrences of beachrock deposits in Krakal-Sadranan, Yogyakarta, and (ii) develop cementation method based on ureolytic bacteria derived from naturally formed beachrock as an alternative technique for stabilizing sandy coastal environments. The experiment employed bacteria isolated from the Yogyakarta coast (Pseudoalteromonas tetradonis). The outcomes revealed that the sample treated with a gradually injected cementation solution achieved an approximate strength of 10 MPa after a 14-day treatment.

Coral reef rehabilitation sites selection based on seasonal waves and environmental quality as ecological supporting system on Mandangin Island

The natural rehabilitation process in the coral reef ecosystem requires a genuinely suitable environment minimum disturbance marine threats. Baby coral is a fragile organism used to increase the quantity of coral reef cover during rehabilitating coral reef ecosystems— determination for coral rehabilitation sites with minimum marine threats for increasing success. The destructive fishing around Mandangin Island induces a declination of live coral coverage. On average, live coral cover around Mandangin Islands is under 10 percent. However, there is high coverage of dead coral that is still functional as a shelter for reef fish in the southeast Mandangin Islands. The dead reef structure can still prevent the coastal area from high seasonal waves hitting the southeast coast from July to September. A large-scale reef structure makes it difficult for small-scale fishers to disturb the rehabilitation area. Water quality indicated that east-to-south coastal areas are considered coral rehabilitation sites. Unique water quality affects the coral adaptation on Mandangin Island. East to Southeast coastal areas are considered coral reef rehabilitation locations based on coral reef cover, water quality, and seasonal waves. The limitation of the coral fragment from Mandangin Islands became the most concerned variable for rehabilitation and reef-structure design to anticipate the seasonal waves.

Dipeptide-Based Photoreactive Instant Glue for Environmental and Biomedical Applications.

Nature-inspired smart materials offer numerous advantages over environmental friendliness and efficiency. Emulating the excellent adhesive properties of mussels foot proteins, where the lysine is in close proximity with the 3,4-dihydroxy-l-phenylalanine (DOPA), we report the synthesis of a novel photocurable peptide-based adhesive consisting exclusively of these two amino acids. Our adhesive is a highly concentrated aqueous solution of a monomer, a cross-linker, and a photoinitiator. Lap-shear adhesion measurements on plastic and glass surfaces and comparison with different types of commercial adhesives showed that the adhesive strength of our glue is comparable when applied in air and superior when used underwater. No toxicity of our adhesive was observed when the cytocompatibility on human dermal fibroblast cells was assessed. Preliminary experiments with various tissues and coral fragments showed that our adhesive could be applied to wound healing and coral reef restoration. Given the convenience of the facile synthesis, biocompatibility, ease of application underwater, and high adhesive strength, we expect that our adhesive may find application, but not limited, to the biomedical and environmental field.