Multiple injection is one of the advanced technologies employed in modern diesel engines to improve combustion efficiency, reduce pollutant emissions, and minimize combustion noise. The application of multiple injection in marine diesel engines differs from its use in vehicles or heavy-duty engines, as it is not commonly combined with Exhaust Gas Recirculation (EGR). However, there is a scarcity of studies specifically examining the combustion characteristics of medium-speed marine diesel engines utilizing multiple injection. Given the large space scale of marine diesel engine, a constant volume chamber with a visible diameter of 240 mm was used, and an injector with a nozzle diameter of 0.465 mm was employed in the experiment. The spray development and combustion process were recorded by Mie-scattering and flame natural luminosity imaging respectively. Both conventional and double injection combustion processes were analyzed in detail. The results show that although the liquid phase spray does not fully penetrate to the cylinder wall in marine diesel engines, the flame penetrates to the cylinder wall rapidly, the flame burns near the wall almost throughout the entire combustion duration. The combustion characteristics of the double injection are significantly different, the flame propagation speed of the pilot and main injection fuel is one-third to one-half of that of a single injection with a long duration. For single injection spray with long injection duration, the flame penetration velocity is determined by the sequential ignition velocity of the fuel. While the flame penetration velocity for sprays with short injection duration mainly depends on the jet penetration velocity. The investigation on the impact of dwell time, fuel distribution, and injection pressure on the combustion process of double injection also provides valuable insights for optimizing multiple injection strategies.