Controlled Release Properties Research Articles

Responses of microalgae under different physiological phases to struvite as a buffering nutrient source for biomass and lipid production

Microalgae cultivation for biodiesel production is promising, but the high demand for nutrients, such as nitrogen and phosphorus, remains a limiting factor. This study investigated effects of struvite, a low-cost nutrient source, on microalgae production under different physiological phases. Changes in element concentrations were determined to characterize the controllable nutrient release properties of struvite. Results showed that nutrient elements could be effectively supplemented by struvite. However, responses of microalgae under different growth stages to struvite varied obviously, achieving the highest biomass (0.53 g/L) and the lowest (0.32 g/L). Moreover, the microalgal lipid production was obviously increased by adding struvite during the growth phase, providing the first evidence that struvite could serve as an alternative buffering nutrient source to culture microalgae. The integration of microalgae cultivation with struvite as a buffering nutrient source provides a novel strategy for high ammonia nitrogen wastewater treatment with microalgae for biodiesel production.

M6P-modified solid lipid nanoparticles loaded with matrine for the treatment of fibrotic liver

Liver fibrosis is a key pathological process shared by the progression of various chronic liver diseases. Treatment of liver fibrosis can effectively block the occurrence and development of hepatic cirrhosis or even carcinoma. Currently, there is no effective drug delivery vehicle for curing liver fibrosis. In this study, we designed matrine (MT)-loaded mannose 6-phosphate (M6P) modified human serum albumin (HSA) conjugated solid lipid nanoparticles (SLN), named M6P-HSA-MT-SLN for treatment of hepatic fibrosis. We demonstrated that M6P-HSA-MT-SLN exhibited controlled and sustained release properties and good stability over 7 days. The drug release experiments showed that M6P-HSA-MT-SLN exhibited slow and controlled drug release characteristics. In addition, M6P-HSA-MT-SLN showed a significant targeted ability to fibrotic liver. Importantly, in vivo studies indicated that M6P-HSA-MT-SLN could significantly improve histopathological morphology and inhibit the fibrotic phenotype. In addition, in vivo experiments demonstrate that M6P-HSA-MT-SLN could reduce the expression of fibrosis markers and alleviate the damage of liver structure. Hence, the M6P-HSA-MT-SLN provide a promising strategy to deliver therapeutic agents to fibrotic liver to prevent liver fibrosis.

Enhanced Antifungal Activity of Electrosprayed Poly (vinyl alcohol)/Chitosan Nanospheres Loaded with Sage Essential Oil on the Viability of Aspergillus niger and Botrytis cinerea

AbstractIn this study, sage essential oil (SEO) loaded nanoparticles were tested in terms of their antifungal properties. In this respect, the electrospray method was employed to fabricate SEO‐loaded nanoparticles (SNPs) by encapsulation of different concentrations (0.0, 0.25 %, 0.5 %, and 1 % v/v) of SEO within poly(vinyl alcohol)/chitosan‐based nanoparticles (PVA/Chi NPs). The FTIR, DSC, and SEM analyses divulged that SEO could be successfully encapsulated within nanoparticles, possessing a spherical shape and high thermal stability. The encapsulation efficiency values of SNPs at different concentrations varied between 66.1 and 73.3 %. It was determined that SNPs released 62.56 % of their SEO content within 350 minutes. Moreover, the antifungal activity of SEO against Aspergillus niger and Botrytis cinerea could be increased significantly (p<0.05) after it was encapsulated into PVA/Chi NPs. Based on these findings, suggest that SNPs could be used in food preservation applications as a long‐term and natural preservative given their controlled release properties.

Synthesis, and control release properties of Zirconium –AA7075 - beta naphtha oxy acetate nanocomposites

In this research, Beta naphtha oxy acetate (BNOA) was used as the guest anion, and Zirconium-aluminum 7075 layered double hydroxide (Zr-AA7075-LDH) was employed as the inorganic host, to create an organic–inorganic nanohybrid nanocomposite using the co-precipitation process. At a BNOA concentration of 0.08 M and a Zr/AA7075, R = 2 M ratio, a stable nanohybrid nanocomposite was produced. The formation of a Zr-AA7075-BNOA nanocomposite, wherein the nitrate ion interlayer gap in layered double hydroxide grew from 8.8 to 19.6 Å, demonstrates the successful intercalation of Beta naphtha oxy acetate into these spaces. Moreover, it was discovered that the Brunauer-Emmett-Teller surface area for Zr-AA7075-LDH and Zr-AA7075-BNOA nanocomposites rose from 4.23 to 45.84 m2 g−1. The generated nanocomposite is a mesoporous material with a BJH mean pore diameter of199 Å. CHNS analysis of the sample revealed that the Zr-AA7075- Beta naphtha oxy acetate nanocomposite material is 37.4% (w/w) depend on the carbon content. Pseudo-second order kinetics took over after 200 min of BNOA deintercalation from the Zr-AA7075 lamella being driven by zeroth and first order kinetics. It appears that the organic acid herbicide BNOA can be manufactured using layered double hydroxide as a carrier, as shown by the study's findings.

Study on the controlled release properties of modified multi-walled carbon nanotubes on sulforaphane

Study on the controlled release properties of modified multi-walled carbon nanotubes on sulforaphane

Advanced injectable hydrogels for bone tissue regeneration.

Diseases or defects of the skeleton are hazardous because of their specificity and intricacy. Bone tissue engineering has become an important area of research that offers promising new tools for making biomimetic hydrogels that can be used to treat bone diseases. New hydrogels with a distinctive 3D network structure, high water content, and functional capabilities are ranked among the most promising candidates for bone tissue engineering. This makes them helpful in treating cartilage injury, skull deformity, and arthritis. This review will briefly introduce the variety of biocompatible functional hydrogels used in cell culture and bone tissue regeneration. Many gel design concepts, such as crosslinking procedures, controlled release properties, and alternative bionic methodology, were stressed regarding injectable hydrogels to form bone tissue. Hydrogels manufactured from biocompatible materials are a promising option for minimally invasive surgery because of their adaptable physicochemical qualities, ability to fill irregularly shaped defect sites, and ability to grow hormones or release drugs in response to external stimuli. Also included in this overview is a quick rundown of the more practical designs employed in treating bone disorders. Essential details on injectable hydrogel scaffolds for bone tissue regeneration are described in this article.

Immunological evaluation of a recombinant vaccine delivered with an analogous hyaluronic acid chitosan nanoparticle-hydrogel against Toxoplasma gondii in mice

BackgroundToxoplasma gondii (T. gondii) is not only a threat to the public health but it also poses adverse impacts on the livestock industry. This study aimed to develop a recombinant vaccine composed of T. gondii microneme protein 6 (TgMIC6) and T. gondii rhoptry protein 18 (TgROP18). The vaccine was delivered with a novel vector, named analogous hyaluronic acid chitosan nanoparticle-hydrogel (AHACNP-HG) and its immune protection was evaluated. MethodsThe recombinant MIC6 and ROP18 proteins were obtained by affinity chromatography and loaded onto AHACNP-HG by magnetic stirring. The characterizations of AHACNP-HG were investigated, including its structure, rheological property, nanoparticle size and zeta potential, its ability to release protein in vitro and toxicology in vivo. The immunological and anti-infection effects of AHACNP-HG/rMIC6/rROP18 were examined in the mice model. ResultsAHACNP-HG presented a characteristic of composite system and possessed biosecurity with excellent protein control-release property. AHACNP-HG/rMIC6/rROP18 vaccine enhanced a mixed Th1/Th2 cellular immune response accompanied by an increased level of the cytokines, IFN-γ and IL-10. It also provoked a stronger humoral immune response. Additionally, after challenge with T. gondii tachyzoite, AHACNP-HG/rMIC6/rROP18 inoculation prolonged the survival time of mice. ConclusionOur data indicated that mixed rMIC6 and rROP18 induced strong immune response and played a certain protective role in controlling T. gondii infection, and the novel adjuvant AHACNP-HG improved modestly some immunogenicity properties in mouse model, which indicated that it can be used as a novel delivery system in vaccine development.

Curcumin-based nanoformulations alleviate wounds and related disorders: A comprehensive review.

Despite numerous advantages, curcumin's (CUR) low solubility and low bioavailability limit its employment as a free drug. CUR-incorporated nanoformulation enhances the bioavailability and angiogenesis, collagen deposition, fibroblast proliferation, reepithelization, collagen synthesis, neovascularization, and granulation tissue formation in different wounds. Designing nanoformulations with controlled-release properties ensure the presence of CUR in the defective area during treatment. Different nanoformulations encompassing nanofibers, nanoparticles (NPs), nanospray, nanoemulsion, nanosuspension, nanoliposome, nanovesicle, and nanomicelle were described in the present study comprehensively. Moreover, for some other systems which contain nano-CUR or CUR nanoformulations, including some nanofibers, films, composites, scaffolds, gel, and hydrogels seems the CUR-loaded NPs incorporation has better control of the sustained release, and thereby, the presence of CUR until the final stages of wound healing is more possible. Incorporating CUR-loaded chitosan NPs into nanofiber increased the release time, while 80% of CUR was released during 240 h (10 days). Therefore, this system can guarantee the presence of CUR during the entire healing period. Furthermore, porous structures such as sponges, aerogels, some hydrogels, and scaffolds disclosed promising performance. These architectures with interconnected pores can mimic the native extracellular matrix, thereby facilitating attachment and infiltration of cells at the wound site, besides maintaining a free flow of nutrients and oxygen within the three-dimensional structure essential for rapid and proper wound healing, as well as enhancing mechanical strength.

Intranasal Polymeric and Lipid-Based Nanocarriers for CNS Drug Delivery

Nanomedicine is currently focused on the design and development of nanocarriers that enhance drug delivery to the brain to address unmet clinical needs for treating neuropsychiatric disorders and neurological diseases. Polymer and lipid-based drug carriers are advantageous for delivery to the central nervous system (CNS) due to their safety profiles, drug-loading capacity, and controlled-release properties. Polymer and lipid-based nanoparticles (NPs) are reported to penetrate the blood-brain barrier (BBB) and have been extensively assessed in in vitro and animal models of glioblastoma, epilepsy, and neurodegenerative disease. Since approval by the Food and Drug Administration (FDA) of intranasal esketamine for treatment of major depressive disorder, intranasal administration has emerged as an attractive route to bypass the BBB for drug delivery to the CNS. NPs can be specifically designed for intranasal administration by tailoring their size and coating with mucoadhesive agents or other moieties that promote transport across the nasal mucosa. In this review, unique characteristics of polymeric and lipid-based nanocarriers desirable for drug delivery to the brain are explored in addition to their potential for drug repurposing for the treatment of CNS disorders. Progress in intranasal drug delivery using polymeric and lipid-based nanostructures for the development of treatments of various neurological diseases are also described.

Preparation of pH-sensitive chitosan-deoxycholic acid-sodium alginate nanoparticles loaded with ginsenoside Rb1 and its controlled release mechanism

Ginsenoside is a natural extract of the genus ginseng, which has tumor preventive and inhibiting effects. In this study, ginsenoside loaded nanoparticles were prepared by an ionic cross-linking method with sodium alginate to enable a sustained slow release effect of ginsenoside Rb1 in the intestinal fluid through an intelligent response. Chitosan grafted hydrophobic group deoxycholic acid was used to synthesize CS-DA, providing loading space for hydrophobic Rb1. Scanning electron microscopy (SEM) showed that the nanoparticles was spherical with smooth surfaces. The encapsulation rate of Rb1 enhanced with the increase of sodium alginate concentration and could reach to 76.62 ± 1.78 % when concentration was 3.6 mg/mL. It was found that the release process of CDA-NPs was most consistent with the primary kinetic model which is a diffusion-controlled release mechanism. CDA-NPs exhibited good pH sensitivity and controlled release properties in buffer solutions of different pH's at 1.2 and 6.8. The cumulative release of Rb1from CDA-NPs in simulated gastric fluid was <20 % within 2 h, while could release completely around 24 h in the simulated gastrointestinal fluid release system. It was demonstrated that CDA3.6-NPs can effectively control release and intelligently deliver ginsenoside Rb1, which is a promising alternative way for oral delivery.

Amyloid Fibrils Enhance the Topical Bio-Adhesivity of Liquid Crystalline Mesophase-Based Drug Formulations.

Despite their distinctive secondary structure based on cross β-strands, amyloid fibrils (AF) are stable fibrous protein aggregates with features similar to collagen, one of the main components of the extracellular matrix, and thus constitute a potential scaffold for enhancing cell adhesion for topical applications. Here, the contribution of AF to skin bio-adhesivity aiming toward topical treatments is investigated. Liquid crystalline mesophase (LCM) based on phytantriol is formulated, with the aqueous phase containing either water or a solution of 4wt% amyloid fibrils. Then resveratrol is added as a model anti-inflammatory molecule. The developed LCM presents a double gyroid Ia3d mesophase. The incorporation of AF into the LCM increases its bio-adhesive properties. In vitro release and ex vivo permeation and retention confirm the controlled release property of the system, and that resveratrol is retained in epidermis and dermis, but is also permeated through the skin. All formulations are biocompatible with L929 cells. The in vivo assay confirms that systems with AF lead to a higher anti-inflammatory effect of resveratrol. These results confirm the hypothesis that the incorporation of AF in the LCM increases the bio-adhesiveness and efficiency of the system for topical treatment, and consequently, the therapeutical action of the encapsulated drug.

Plasma etching effect on the molecular structure of chitosan-based hydrogels and its biological properties

To reasonably use hydrogels in healthcare field, this study four kinds of chitosan (CTS)-based hydrogels with different molecular structures. With plasma etching, the morphology, chemical groups' proportion, and hydrophilicity of the hydrogel surface were changed. At 40 min of modification, the ratios of CO and NH2 on the CTS40-based hydrogel surface increased and reached their maximum values of 40.31 % and 89.17 %, respectively. Combined with the changes in hydrophilic chemical groups and the hydrogel's network structure, the hydrogel surface's wettability changed after plasma etching. From the results, CTS40-based hydrogel showed the lowest contact angle (77.40 ± 3.89°) with 80 min modification due to its dense network structure of CTS and appropriate ratio of hydrophilic groups on the surface. With these molecular structural changes, the antibacterial properties of CTS-based hydrogels against Staphylococcus aureus were improved. Moreover, the functional components delivery system coating with these CTS-based hydrogels showed colon-site controlled-release property. The hydrogels also facilitated the growth of Caco2 and Hic cells, which had 72.74 %–453.27 % cell viability of Caco2 cells on the surface. Therefore, the antibacterial property and biocompatibility of plasma modified CTS-based hydrogels have been demonstrated. The mechanism between molecular structure changes of CTS with plasma etching and its properties was discussed, which would provide a promising carrier material for utilizing healthcare field.

Oral cyclodextrin supramolecular for ulcerative colitis treatment through macrophages targeting / ROS response-based accurate on-demand drug release strategy

Cyclodextrins (CDs) have been applied widely as an oral micro-nano drug delivery material for the treatment of ulcerative colitis (UC) because of their stability around the stomach. However, the further application of CDs is hindered due to their inherent poor targeting and controlled release properties. In this study, CD-supramolecular nanoparticles (HA-CsT@RH) were developed based on the structural characteristics of CDs (e.g., multi-group modifiability and hydrophobic cavity) to encapsulate rhein (RH) for oral delivery against UC. The HA-CsT@RH showed an average size of 154.70 ± 1.80 nm and exhibited high encapsulation efficiency (95.55 ± 2.37 %). When incubated in simulated gastric fluid for 2 h, HA-CsT@RH only released 20.3 ± 0.47 % RH due to the protection of supramolecular effects of CDs. However, RH was rapidly released from HA-CsT@RH (released 66.3 ± 2.50 %) after H2O2 was treated with the assistance of the ROS-sensitive properties. Moreover, HA-CsT@RH were taken up by macrophages in 1.5 times higher amounts than preparations which did not have CD44-targeted effects. Notably, the results showed that HA-CsT@RH with an accurate on-demand drug release strategy achieved the best anti-inflammatory and antioxidant effects, and they are expected to be a promising carrier in the UC treatment.

Preparation of High Bio-Content Polyurethane Coatings from Co-Liquefaction of Cellulosic Biomass and Starch for Controlled Release Fertilizers

To manufacture high bio-content degradable polyurethane-coated fertilizer, the co-liquefaction of corn straw and starch was carried out to convert more biomass into bio-polyol so as to substitute petroleum-based polyol. The effect of the corn straw to starch ratio on liquefaction behavior was mainly investigated by monitoring acid value, hydroxyl value, and liquefaction rate. Both chemical structures and properties of bio-polyols and their coatings were characterized by Fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), etc. The results indicated that adding a certain amount of starch to corn straw for co-liquefaction could contribute to a higher hydroxyl content of bio-polyol than that from sole biomass. Compared to molecular weight, the hydroxyl value was a more significant index to ensure coating quality. The incorporation of starch segments to bio-polyol led to good thermal stability, limited residual small molecules, and high crosslinking density of the coating. The controlled-release ability of coated urea prepared by co-liquefied bio-polyol was better than that of corn-straw-based bio-polyol, and the optimal ratio of corn straw to starch was 3:1. Overall, this study provides a new route to fabricate corn straw as a cost-effective and degradable coating with good controlled-release properties, which benefits solid biomass recycling.

Carboxymethylpachymaran-Coated Zein Nanoparticles for Oral Delivery of Curcumin: Formation, Characterization, Physicochemical Stability, and Controlled Release Properties

The instability and poor solubility of curcumin limit its intake in the human body. In this work, curcumin-encapsulated carboxymethylpachymaran/zein composite nanoparticles (ZMCNPs) were prepared by anti-solvent precipitation, and the morphology of composite nanoparticles was confirmed by transmission electron microscopy, and the interaction between carboxymethylpachymaran, zein, and curcumin was explored by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and differential scanning calorimetry. The encapsulation efficiency of curcumin by ZMCNPs (83.2%) was significantly higher than that of zein-curcumin nanoparticles (ZCNPs) (44.9%). The ZMCNPs also exhibit high ionic strength tolerance and light, heat, and storage stability. The in vitro release showed that ZMCNPs could effectively prevent the premature release of curcumin in the stomach (9.5%), while curcumin was better sustained in the small intestine stage (47.9%). Finally, the result of cytotoxicity assay in IEC-6 cells and HT-29 cells indicated the good biocompatibility of the ZMCNPs. Therefore, our study may provide some novel options for delivering biologically active substances.

Ca-DEX biomineralization-inducing nuts reverse oxidative stress and bone loss in rheumatoid arthritis.

Rheumatoid arthritis (RA) is a common autoimmune disease, and the inflammatory response during its development can lead to joint cartilage and bone damage up to disability. Dexamethasone (DEX) can effectively alleviate the inflammatory response in RA, but the severe adverse effects that occur after its long-term administration limit its clinical development. Herein, we propose a Ca-DEX biomineralization-inducing nut (CaCO3-DEX) with controlled release properties for mitigating the toxic side effects of DEX in RA treatment, especially the damage to cartilage and bone. CaCO3-DEX releases the drug and Ca2+ preferentially in an inflammatory environment. Both in vitro and in vivo studies demonstrate that CaCO3-DEX significantly reduces the secretion of pro-inflammatory factors and inhibits ROS production in vitro, as well as demonstrates superior pro-biomineralization and osteogenic differentiation potential. In the collagen-induced rheumatoid arthritis model (CIA model), CaCO3-DEX significantly reduces the clinical score of arthritis in mice, and the imaging results show a noticeable relief of edema and bone erosion in CIA model mice treated with CaCO3-DEX, while inflammatory factors at the injury areas are significantly reduced, which provides favorable protection to cartilage and bone.

Increased radical scavenging activity of thymoquinone and l-ascorbic acid dual encapsulated in palmitoyl-chitosan nanoparticles in a human normal lung fibroblast, MRC-5 due to synergistic antioxidative effects.

Less effective antioxidant supplementation in combating free radicals is often related to the lack of the formulation of carriers. The antioxidant may be one of the most powerful substances but is marred by poor uptake by cells when the carrier degraded and dissolved too rapidly. Nanoparticle (NP) systems are promising in overcoming the problem since they provide high surface area to enhance encapsulation and release efficiency. With the right selection of material, NP carriers could function as constructive antioxidant cargos. Generally, NPs carry only one active ingredient; this study, however, utilized chitosan nanoparticles (CNPs) and hydrophobically modified palmitoyl-chitosan nanoparticles (PCNPs) that were dual encapsulated with antioxidants of different polarities, namely, hydrophobic thymoquinone (TQ) and hydrophilic l-ascorbic acid (LAA) to evaluate their combination effects in scavenging free radicals. The antioxidants followed zero-order release kinetics with a controlled release manner for about 48 h. The interaction effects between TQ and LAA loaded in the NP systems were determined by classical isobologram (CI) values. The CI values were derived by a diphenyl picrylhydrazyl (DPPH) assay, a radical scavenging activity assay. Combined TQ and LAA had CI values of less than one, with a lower value in the PCNP system than in the CNP system. This indicates that the interaction between those antioxidants showed higher synergistic effects in PCNPs, which enhanced the DPPH radical scavenging activities. The antioxidative potential of compound(s) encapsulated in the PCNP carrier was further experimented by a reactive oxygen species (ROS) assay on a human normal lung fibroblast cell line (MRC-5) as lung is one of the organs with high accumulation of free radicals. About 48 h post treatment, the dual-loaded TQ and LAA in PCNPs showed the lowest ROS level in comparison to single-loaded antioxidants and bare antioxidant delivery. The hydrogen peroxide (H2O2) radical scavenging was influenced by both the controlled release property of the PCNP system and the synergy between TQ and LAA. In short, dual-loaded TQ and LAA in the hydrophobically modified PCNP had effectively depicted the capability of a single CS-based nanocarrier to hold more than one compound at a time to function as a potent radical scavenger.

3D printed matrix solid forms: Can the drug solubility and dose customisation affect their controlled release behaviour?

The use of 3D printing in pharmaceutics has grown over the last years, along with the number of studies on the impact of the composition of these formulations on their pharmaceutical and biopharmaceutical properties. Recently, we reported the combined effect of the infill percentage and the presence of a pore former on the drug release behaviour of 3D printed matrix solid forms prepared by fused deposition modelling. However, there are some open questions about the effect of the drug solubility and the size of these dosage forms on their controlled release properties. Therefore, we produced poly(Ɛ-caprolactone) filaments containing different soluble forms of dexamethasone (free acid, DEX; acetate ester, DEX-A; and phosphate salt, DEX-P), which showed suitable mechanical properties and printability. 3D printed solid forms were produced in two different sizes. The formulations composed of DEX-P released about 50% of drug after 10 h, while those containing DEX or DEX-A released about 9%. The drug release profiles from the 3D printed forms containing the same drug form but with different sizes were almost completely overlapped. Therefore, these 3D printed matrix solid forms can have their drug content customised by adjusting their size, without changing their controlled release behaviour.

A novel multifunctional carrier with magnetic-NIR luminescent-microwave heating characteristics for drug delivery

We constructed a novel core–shell structured Fe3O4@WO3-x(x = 0∼1)@GdF3: Yb/Er nanoparticles with magnetic-NIR luminescent-microwave heating characteristics used as drug carrier to investigate the loading and controllable release properties of the chemotherapeutic drug doxorubicin (DOX). The porous surface of Fe3O4@WO3-x(x = 0∼1)@GdF3:Yb/Er nanoparticles can store DOX molecules by means of physical adsorption. The Fe3O4 core and GdF3: Yb/Er shell functioned successfully for magnetic targeting (1.40 emu•g−1) and NIR fluorescence imaging (NIR 650–850 nm), respectively. The introduction of WO3-x(x = 0∼1) with LSPR effect enhanced the luminescence of near-infrared region of Fe3O4@WO3-x(x = 0∼1)@GdF3:Yb/Er nanoparticles successfully. In addition, the WO3-x(x = 0∼1) acts as a good microwave absorber with excellent microwave thermal response property for microwave triggered drug release (the DOX release of 12% under microwave irradiation for 10 s outclass the 2% within 1 h without microwave irradiation release). The release profile could be controlled by the duration and number of cycles of microwave application. Moreover, it can monitor the drug release process in real time under the guidance of near infrared imaging, which is convenient to evaluate the therapeutic effect. This work provides a new idea for realizing the visual real-time dynamic monitoring of the chemotherapy process and realizing “positioning-timing-quantitative” administration, thus improving the chemotherapy effect.

Polycaprolactone Electrospun Nanofiber Membrane with Sustained Chlorohexidine Release Capability against Oral Pathogens.

Multiple-pathogen periodontal disease necessitates a local release and concentration of antibacterial medication to control inflammation in a particular location of the mouth cavity. Therefore, it is necessary to effectively load and deliver medicine/antibiotics to treat numerous complex bacterial infections. This study developed chlorhexidine (CHX)/polycaprolactone (PCL) nanofiber membranes with controlled release properties as periodontal dressings to prevent or treat oral disorders. Electrostatic spinning was adopted to endow the nanofiber membranes with a high porosity, hydrophilicity, and CHX loading capability. The release of CHX occurred in a concentration-dependent manner. The CHX/PCL nanofiber membranes exhibited good biocompatibility with human periodontal ligament stem cells, with cell viability over 85% in each group via CCK-8 assay and LIVE/DEAD staining; moreover, the good attachment of the membrane was illustrated by scanning electron microscopy imaging. Through the agar diffusion assay, the nanofiber membranes with only 0.075 wt% CHX exhibited high antibacterial activity against three typical oral infection-causing bacteria: Porphyromonas gingivalis, Enterococcus faecalis, and Prevotella intermedia. The results indicated that the CHX/PCL nanofiber holds great potential as a periodontal dressing for the prevention and treatment periodontal disorders associated with bacteria.