ABSTRACT This paper studies the non-singular practical fixed-time neural adaptive control issues for multi-input and multi-output (MIMO) nonlinear systems with non-strict feedback form. Neural networks (NN) are used to estimate the unknown nonlinearities and deal with the problem of an algebraic loop. Under the framework of the backstepping control design, a practical fixed-time adaptive NN control method is developed by using the adding power integration technology. According to the Lyapunov function theory, it is proved that the closed-loop system is practical fixed-time stable, and the system can track the desired reference signal within a fixed time. Finally, the proposed practical fixed-time control method is applied to a multi-motor control platform, which proves the effectiveness of the control method.