Load shedding could be a solution to water accumulation alleviation during the operation of proton exchange membrane fuel cell (PEMFC) engines. In this paper, multiple-step loading reduction strategies based on two laboratory-scale (25 cm2) single PEMFCs (assembled with triple-serpentine and single-serpentine flow field plates on the both electrodes and thus named after cell TS and cell SS respectively) are experimentally discussed and evaluated. The real-time pressure evolution and HFR are recorded, compared and analyzed to characterize water transfer phenomenon among the components. The investigation results suggest that: 1. From the perspective of output voltage and power after load reduction, the number of reduction step has little effect on the output performance; 2. Cell TS exhibits better homogeneous trend of pressure drop although with less water removal quantity comparing with that of cell SS, which is resulted from multi-channel configuration; 3. The cathode pressure drop of cell SS decreases obviously throughout the experiment, indicating the convinced water removal capability of single-serpentine configuration; 4. Constant increase of HFR reflects membrane dehydration when the loading reduces. This paper demonstrates the possibility of removing accumulated liquid water by reducing the load of PEMFCs.