Railway passing traffic, speed, and load have significantly increased over the years, prompting industry stakeholders and researchers to seek an alternative sleeper material that can demonstrate its ability to potentially possess higher in-service bending resistance and be environmentally friendly and durable. To address these needs and due to environmental concerns, kenaf-reinforced polyamide has become of great importance. However, they could not be applied as railway track components because of the non-availability of their performance in this regard. In bridging this gap, this paper focused on fabricating and characterising six different formulations of treated kenaf fibre (TKF, 0–50% at 10% loading interval) reinforced polyamide biocomposites for railway sleeper applications. The result showed that the incorporation of TKF influenced the behaviour of the polyamide with respect to its water absorption, load-carrying capacity, and thermal stability. The result further demonstrated that the load-bearing capacity peaked at TKF 40 wt.%, surpassing conventional wooden and concrete sleepers. However, its water absorption (64-days saturation) behaviour increased significantly between 11%–21% as TKF rose from 10–50 wt.%, as expected due to TKF hydrophilic characteristics. On the other hand, TKF thermal stability was hampered beyond approximately 220°C for all TKF percentages. Kenaf fibre-reinforced polyamide biocomposites have demonstrated their potential for railway sleeper applications as their load-bearing capacity exceeded the minimum recommended AREMA specifications. Despite the milestone achieved, water absorption of kenaf fibre remained high. The development of sustainable and effective materials to meet the changing needs of contemporary railway infrastructure is greatly aided by the insights gained from this study.