Concentrations Of Benzyladenine Research Articles

Indirect shoot regeneration from callus of the shoot tip explants of Gerbera jamesonii Bolus cv. "Festival"

The study was conducted in the Tissue Culture Laboratory, Fadak Agricultural Company, Basrah, Iraq. Mother plants of the “Festival” gerbera cultivar were used for micropropagation by taking the shoot tip explants. The concentration of 2 mgL-1 benzyl adenine (BA) resulted in recording the highest percentage of response to callus induction, reaching 90%, which was significantly superior to the other two treatments, 1 and 1.5mgL-1 BA. The 1 mgL-1 BA treatment was significantly superior in response to indirect shoot generation when compared with the 1.5 and 2 mgL-1 BA treatments. The 1 mgL-1 BA treatment recorded the highest percentage of response to shoot growth induced by callus, which reached 100%. The treatment of callus grown in MS medium supplied with BA concentration of 1 mgL-1 recorded the highest average number of shoots, which reached 10 shoots per 50 mg of callus. The MS medium treatment supplied with a concentration of 0.5 mgL-1 of naphthalene acetic acid (NAA) was significantly superior to the other two treatments of 1 and 1.5 mgL-1 NAA in the percentage of shoot response to root formation. The concentration of 0.5mgL-1 NAA recorded the highest response to root formation, which reached 100%. The treatment of the MS medium supplied with a concentration of 0.5 mgL-1 NAA was significantly superior to the treatments of 1 and 1.5 mgL-1 NAA in the average number of main roots formed on the shoots. This treatment recorded the highest average of main roots, amounting to 3.2 main roots shoot-1. Callus, cultivar, indirect shoot, micropropagation, proliferation, MS salts.

Exogenously applied gibberellic acid and benzylamine modulate growth and chemical constituents of dwarf schefflera: a stepwise regression analysis

Ornamental foliage plants that have a dense appearance are highly valued. One way to achieve this is by using plant growth regulators as a tool for plant growth management. In a greenhouse with a mist irrigation system, a study was conducted on dwarf schefflera, an ornamental foliage plant, which was exposed to foliar application of gibberellic acid and benzyladenine hormones. The hormones were sprayed on dwarf schefflera leaves at 0, 100, and 200 mg/l concentrations, at 15-day intervals in three stages. The experiment was conducted as a factorial based on a completely randomized design, with four replicates. The combination of gibberellic acid and benzyladenine at 200 mg/l concentration had a significant effect on leaf number, leaf area, and plant height. The treatment also resulted in the highest content of photosynthetic pigments. Furthermore, the highest soluble carbohydrate to reducing sugars ratio was observed in treatments of 100 and 200 mg/l benzyladenine, and 200 mg/l gibberellic acid + benzyladenine. Stepwise regression analysis showed that root volume was the first variable to enter the model, explaining 44% of variations. The next variable was root fresh weight, and the two-variable model explained 63% of variations in leaf number. The greatest positive effect on leaf number was related to root fresh weight (0.43), which had a positive correlation with leaf number (0.47). The results showed that 200 mg/l concentration of gibberellic acid and benzyladenine significantly improved morphological growth, chlorophyll and carotenoid synthesis, and reducing sugar and soluble carbohydrate contents in dwarf schefflera.

EFFECT OF BIO HEALTH, TECAMIN MAX AND BENZYL ADENINE ON GROWTH CHARACTERISTICS OF SWEET ORANGE SEEDLINGS

This investigation was aimed to study the effect of soil application of Bio health and foliar application with Tecamin max and Benzyl adenine on growth characteristic of sweet orange (Citrus sinensis L.) seedlings during two successive seasons (2020 and 2021). Bio health was added with three concentrations (0, 6 and 12 g.L-1) to the soil and foliar application of Tecamin max with three concentration (0, 5 and 10 ml.L-1), Benzyl adenine with three concentration (0, 100 and 200 mg. L-1) on the sweet orange seedlings which were brought from private nursery in Duhok city and have two- years old and nearly uniform in growth vigor. The results proved that all parameters such as increases in plant high, stem diameter, branch numbers, single leaf area, total chlorophyll as soon as leaves carbohydrate and leaves dry weight in both seasons were increased significantly as compared with control, accept Tecamin max had no significant effect on leaves carbohydrates and leaves dry weight, Benzyl adenine no significant effect on leaves dry weight on both seasons, Furthermore, combination among high concentration of Bio health, Tecamin max and Benzyl adenine improved all parameters in comparison with the control.

Effect of the source of the plant part and plant growth regulators on the establishment and multiplication of vegetative branches of Rosmarinus officinalis L. in vitro

This search was carried out in the plant tissue culture laboratory at the College of Agriculture at the University of Kerbala from 2021 to 2022. The study included the use of in vitro technology in the use of plant parts and different combinations in the emergence and multiplication of farms vegetative branches of rosemary, the study was carried out in two stages after performing the sterilization process: the first included the emergence of vegetative farms by planting the growing tops and side nodes on the MS nutrient medium with different concentrations (0 , 1, 2, 3 mg.L-1) BA, and the second stage was carried out by cultivating the growths resulting from the nodes grown in the previous stage on the (MS) media, that was prepared with various concentrations (0, 1, 2, 3 mg.L-1) BA and ( 0, 0.1, 0.2, 0.4mg.L-1) NAA, The results of the study showed the superiority of the apical, as it achieved a response rate of 57.5% compared to the lateral shoots, which recorded a response rate of 37.5%, and the con. of 1 mg.l-1 BA was superior and achieved a response rate of 80% comparison with the neutral treat that earn a response rate It reached 15%, and the results showed that the same concentration of benzyl adenine at a concentration of 2 mg.l-1 achieved of higher average in the number and length of branches (4.74 branches.plantlet-1, 4.12 cm), while the concentration achieved 3 mg .l- 1 of it had the higher rate of leaves number and the Fresh and Dry weight of the branches (15.24 leaves.plantlet-1, 2986 mg and 1823 mg), respectively, comparison with the neutral treat that achieved the lower average, and the concentration 0.2 mg.L-1 NAA exceeded in achieving The highest average number of branches, leaves, fresh &dry weight of branches was (4.16 branches.plantlet-1 and 14.21 leaves.plantlet-1, 2606 mg and 1594 mg) respectively, while the concentration exceeded 0.4 mg.L-1 NAA in achieving the highest rate The length of the branches reached 3.74 cm. All experiments were carried out using CRD (utterly randomized design). Keywords: explants type ; plant growth regulators ; micro propagation ; rosemary ; tissue culture

Effect of spraying Benzyl adenine (BA) and adding nitrogen fertilizer on yield of Avena sativa L.

The field experiment was conducted in the second agricultural research station located in the Al-Bandar area, which is affiliated with the College of Agriculture at Al-Muthanna University. This Study was conducted during the winter season 2022-2023 was conducted during the 2022-2023 winter agricultural season. The experiment investigated the relationship between two factors. The first factor is nitrogen, which was applied at four distinct four levels (0, 40, 80, and 120 kg N ha-1). After emergence, during the elongation stage, and at the Booting stage, nitrogen was applied in three separate batches. Benzyl adenine is the second factor examined in this study. Four Different concentrations of benzyl adenine were applied to the plant at 0, 100, 150, and 200 mg L-1. The benzyl adenine was applied in two Different concentration, with the first batch being applied during the elongation phase and the second batch being applied during the Booting phase. The application consisted of completely saturating the plant with a spray. The experiment was carried out with randomized complete block design using split plot with three replicates. The Main plots were sprayed with varied concentrations of benzyl adenine, while the Sub- plots received varying levels of nitrogen fertilization. The results showed Significant differences in the Nitrogen application, especially in the Significant of 120 kgN.ha-1 In the following characteristics (number of grains Per panicle, weight of 1,000 grains, grains yield, biological yield), it gave averages of 105.6 grains panicle-1 , 38.36 g, 6.27 ton ha-1, 25.41 ton ha-1), and at 40 kg N ha-1, it was greater than the number of panicle of 429.8 m2. The results also indicated significant effect in the spraying of Benzyl adenine and the highest average treatment of 200 mg L-1 (1,000 grain weight) and gave an average of 37.68 g The interaction between factors under study showed the combination (120 kg N ha-1 x 200 mg L-1 BA) gave averages of 41.49 g in grain weight.

Effect of 2,4-D and NAA in Callus Induction and Differentiation from Different Explants of Moringa Oleifera Lam

This study was carry out in the Laboratory of Plant Cells and Tissue Culture / Department of Forestry Sciences in the College of Agriculture and Forestry / University of Mosul during the year 2021-2022 to study the effect of adding different concentrations of 2,4-Dichloro-Phenoxyacetic acid (2,4-D) or Naphthalene Acetic Acid (NAA) to the MS medium in the induction of callus from different parts of the Moringa oleifera Lam. and then study the effect of different concentrations of 6-Benzyladenine (BA) and Kinetin (Kin) on the differentiation of callus into buds and embryogenesis. Concerning callus induction, the results showed that 2,4-D or NAA in the nutrient medium was necessary after three weeks of cultivation 2,4-D was superior to NAA in the percentage of callus induction, as callus induction occurred by 100% of the explants cultured in the media supplemented with 0.1 mg.l−1 2,4-D. On the other hand, the parts (apical buds, single node, parts of the leaf blade, epicotyl, cotyledon, hypocotyl) differed significantly among themselves in the percentage of their ability on callus induction, as the cotyledons gave the highest rate of induction, amounting to 80%. The shoots resulting from the callus rooted after four weeks of cultivation in media supplemented with different concentrations of IBA at a rate of 100%, regardless of the concentration of IBA. The study proved the existence of a genetic match between the mother plants and the plants produced from the callus induction from them.

Some Agronomical and Physiological Characteristics of Sang Yod Phatthalung Rice from In Vitro Regenerated Plant After Transfer to Clay Soil in Field Conditions

Sang Yod Phatthalung rice is indica rice and grown as Geographical Indications (GI) in Patthalung province. Its dark red color seed contains high antioxidant activity that helps reduction in the risk of some chronic diseases such as diabetes, cancer, and cardiovascular diseases. So far, yield per planting area of this rice is still low. Thus, application of PGRs might play an alternative role in agronomical and physiological characteristics leading to improving yield of this rice. First, shoot tips of Sang Yod Phatthalung rice were cultured to induce multiple shoots and root on liquified PGR-free oil palm culture medium (OPCM) with different concentrations of 6-benzyladenine (BA) (0, 0.5, 1, 1.5 mg/L) alone or in combination with 1-naphthaleneacetic acid (NAA) (0 and 0.5 mg/L). Complete plants were acclimatized to field condition at Phatthalung Rice Research Station (PRRS). After acclimatization for 120 and 140 days, some agronomical and physiological characteristics of rice plant obtained from different PGR containing OPCM were statistically compared. A 2×4 factorial design in RCBD with 4 replication was performed. The results were clearly demonstrated that adding BA in the culture medium increase plant height, number of panicles, panicle length and leaf chlorophyll content. Plantlets obtained from in vitro culturing shoot tip on 0.5 mg/L BA containing medium gave the highest results in average plant height at 189.50 cm, number of panicles at 34.00 panicles, panicle length at 32.50 cm and leaf chlorophyll content at 39.65 SPAD unit. The results from this present study indicated the production of new plant types or characteristics for Sang Yod Phatthalung rice for high yield in future. HIGHLIGHTS The effect of concentrations of PGRs, and culture conditions on agronomical and physiological characteristic were evaluated 5 mg/L BA-derived plants germinated in vitro gave higher results than ex vitro in agronomical and physiological characteristic Addition of 0.5 mg/L BA in the medium increases the plant height, number of panicles, panicle length and leaf chlorophyll content 5 mg/L BA-derived plants in vitro gave the highest plant height, number of panicles, panicle length, and leaf chlorophyll content at 189.50 cm, 34.00 panicles, 32.50 cm, and 39.65 SPAD units, respectively GRAPHICAL ABSTRACT

Application of NAA and BA to Calotropis gigantea (L.) W.T. Aiton in vitro

Crown flower (Calotropis gigantea (L.)) is a weed plant potentially to be used as raw material for textile fibers yet the utilization is not optimal. Efforts to optimize the utilization of C. gigantea are needed. This research aimed to determine the appropriate concentration of plant growth regulators, including NAA (Naphthalene Acetic Acid) and BA (Benzyl Adenine) to stimulate optimal growth of C. gigantea to be used as the basis for in vitro propagation of C. gigantea. The research was arranged in a completely randomized design (CRD). Explants were planted on Murashige and Skoog (MS) medium with various concentrations of NAA (0; 0.5; 1; 1.5; and 2 ppm) and BA (0; 1; 2; 3; and 4 ppm). Based on the research result, the addition of 3 ppm BA followed by increasing the concentration of NAA to 1.5 ppm could increase the number of C. gigantea shoots, and giving 1 ppm BA followed by adding 1.5 to 2 ppm NAA increased shoot height. The best plantlet response was found in media with 0 ppm NAA and 4 ppm BA, which could produce an optimal shoot height of 5.9 cm and a large number of shoots of 5.67 shoots. Increasing NAA concentration retarded root formation and reduced the root length and number of leaves, while the medium without NAA gave the earliest root emergence of 11 days after planting, the number of leaves of 20 strands, and the longest root of 10.9 cm. Applying a single substance BA did not accelerate all the variables observed.

Effects of different plant growth regulators as the foliar application on growth and flower quality of potted rose (Rosa chinensis Jacq. cv. Nhung)

This study was conducted to evaluate the different concentrations of Naphthalene acetic acid (NAA), Benzyladenine (BA) and Gibberellic acid (GA3­) as foliar applications on growth and flower quality of potted rose Nhung (Rosa chinensis Jacq. cv. Nhung). The experiment was constructed in a completely randomized design, that included seven treatments, such as control (sprayed water), NAA, BA and GA3 at two different concentrations of 25 and 50 ppm, in six replications, with one plant/pot for each replication. The chemical solutions were applied three times at an interval of fifteen days. The results revealed that some plant growth regulators increased the growth and flowering time of rose cv. Nhung. Using pre-harvest foliar sprays of NAA and BA at 25 and 50 ppm concentrations showed maximum bud diameter, flower diameter and flowering duration as compared with the control treatment. The concentration of GA3 foliar application at 25 ppm increased plant growth (the height of flowering branches and Spad values) without affecting flower quality values and the flowering duration in comparison with the control treatment.

In Vitro Propagation of Two Tomato Hybrids (Lycopersicon Esculentum Mill.) Via Tissue Culture Technique

This investigation was carried out at the Tissue Culture Lab., Biotechnology Research Center (BTRC) in Tripoli, Libya in 2009 to establish a micro propagation protocol for two hybrids of Tomato in which several factors were studied including the effect of concentration of growth regulator on shoot multiplication, and rooting of explant on MS media. Shoot tips were cultured into (MS medium) with different concentrations of benzyl adenine (BA) + 0.5 mg/l (IAA) compared with MS basal medium as a control treatment. Generally, the results showed that there were no significant differences between several concentrations of (BA+IAA) on the number of leaves and shoot length. The obtained results illustrated was M1 gave high number of shoots/explant, shoot length, and leaves number for two hybrids of Tomato which were 1.90, 3.89, 4.48 respectively compared with the control. The results showed that there were no significant differences between Tomato hybrids on shoot length and the number of shoots. The best result for leaves number was hybrid HH-56 which gave 4.54 leaves. From the foregoing results, MS media supplementing IAA at 0.2 mg/l was the best treatment for rooting stage of hybrid HH-56 and Hybrid AZHAR F1. The plantlets were transferred to plastic cups containing peatmoss + sand at 1:1 (V/V). Acclimatization of plantlets were successfully.

Regeneration of Transgenic Ficus lyrata via Indirect Somatic Embryogenesis and Isolation of Variants for Development of New Cultivars

Ficus lyrata is a popular ornamental foliage plant with unique violin- or guitar-shaped green leaves. In our previous study, a grapevine gene VvMybA1 was introduced into F. lyrata via Agrobacterium-mediated transformation, which resulted in the availability of purple-leaved plants. Since VvMybA1 is a transcription factor, the regeneration of transgenic purple-leaved plants might potentially produce variants with multicolored leaves. The objective of this study was to establish a method for regenerating purple-leaved F. lyrata and determine if variants with different coloration or variegation could be isolated from regenerated populations. Leaf explants derived from a completely purple-leaved transgenic plant were cultured on Murashige and Skoog (MS) basal medium supplemented with different concentrations of 6-benzyladenine (BA) and α-naphthalene acetic acid (NAA). Callogenesis occurred in leaf explants, and a subculture of callus-borne explants on the same medium resulted in callus proliferation and the occurrence of somatic embryos. Somatic embryos were more effectively induced from callus pieces cultured on MS medium supplemented with 8.88 μM of BA and 0.27 μM of NAA. More than 30 embryos were induced per callus piece, and the embryos matured and converted to plantlets. MS medium supplemented with 4.92 μM of indolyl-3-butanoic acid (IBA) greatly improved root development. Plantlets were transplanted into soilless substrate and grown in a shaded greenhouse for morphological evaluation. Nine variants with different degrees of coloration and variegation were isolated from the regenerated populations. Our results suggest that the regeneration of transgenic plants that harbor a transcription factor, such as VvMybA1, could be an additional way of isolating novel variants for the development of new cultivars of ornamental plants.

Effect of Coconut Water and Benzyl Adenine with Different Concentrations on Potato Tissue Culture (Solanum tuberosum L.) Var. Granola

This study aimed to determine the effect of giving coconut water and the right concentration of benzyl adenine in MS media for potato tissue culture of Granola variety. The study was carried out from November to January 2022, in the Plant Tissue Culture Laboratory of the Faculty of Agriculture, Pattimura University, Ambon. This study used a 2-factor completely randomized design (CRD); the first factor was the concentration of coconut water 0 with 100 ml/l, and the second factor was the concentration of BA consisting of 0.1,2,3,4 mg/l. The study was conducted with 7 replicates and each replication consisted of 5 explants in each culture bottle, so that there were 350 cultures as the unit of observation. The variables observed in this study included the number of shoots, the number of internodes, the number of branches, and the number of roots per plantlet. The results showed that the concentration of coconut water 0 and 100 ml/l had an effect on the number of internodes and the number of roots at 4 weeks after planting. Benzyl adenine at 0, 1, 2, 3 and 4 mg/l had an effect on the variable number of shoots at 8 WAP and the number of internodes at 2 - 8 WAP; which also applies to the number of roots. Whereas, the interaction between both factors affected the number of internodes at 4, 6, and 8 weeks, which also applies to the number of roots.

Effect of Explants Type and Plant Growth Regulators on the Stimulation and Multiplication of Vegetative Branches of Trigonella foenum-graecum L. in Vitro

The study included the use of plant tissue culture technology in the use of plant parts and different combinations in the emergence and multiplication of farms of the vegetative branches of the fenugreek plant(1,2,3)mg.l-1 BA, and the second stage was carried out by cultivating the growths resulting from the nodes grown in the previous stage on the MS medium, which was prepared with different concentrations (0, 1, 2, 3) mg.l-1 BA and (0.0,0.1,0.2,0.4)mg.L-1 NAA. The study’s results showed that the concentration of 2 mg.L-1 BA achieved a response rate of 80% compared to the peaks that did not respond. The results also showed that the same concentration of benzyl adenine achieved the highest rate in the number and length of branches, and the number of leaves reached 8.69.plant-1, 4.00 cm, 26.33 leaves.plant) respectively, compared with the control treatment that achieved the lowest rate, and the concentration 0.2 mg.l-1 NAA excelled in achieving the highest rate of the number of branches and leaves reached (7.49 branches. plant-1 and 21.50 leaves.plant-1) respectively. In comparison, the concentration of 0.4 mg.l-1 NAA excelled in achieving the highest average length of branches, reaching 3.92 cm.

Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques

Blood clotting has become one of the most dangerous side effects associated with Corona virus, as well as the high level of cholesterol and triglycerides in the blood. Therefore, it has become necessary to use medicinal plants that are biologically safe and containing anti-clotting compound. Feijoa sellowiana represents a prolific source diverse compounds that may have thrombolytic activity. Therefore, the main research point is the production and scaling up of a target contents that have anticoagulants by using biotechnological techniques; calli production, and bioreactors and assessed their activity through in-vivo study. Murashige and Skoog (MS) medium enriched with varying concentrations of benzyl adenine (BA) and naphthalene acetic acid (NAA) was used to cultivate calli and cell suspension cultures from F. sellowiana seeds. Bioreactors were employed to boost active constituent's production. Moreover, the bioreactor physical factors such as effect of controlled or uncontrolled pH medium were investigated. The leaves of the main plant were extracted by ethanol 70% and polar and non-polar extracts were also prepared. The ethanol extract of calli and cells resulting from bioreactors were also prepared. All prepared extracts were subjected to chemical analysis by HPLC, in-vitro antioxidant assays, in-vivo anticoagulant activity and histopathological examination. Calli and cell suspension cultures were produced by using MS medium fortified with 1 mg/L BA+ 0.1 mg/L NAA. It was found that culturing of cell cultures in a bioreactor with uncontrolled pH and aeration at the value of 0.5 L/min gave the maximum and economical fresh and dry weights of the plants. After evaluation of all extracts; it was found that the calli ethanol extract for each plant was the highest value of total phenolic and total flavonoid contents either quantitatively or qualitatively. All extracts of Feijoa had antioxidant activity. The IC50 of the DPPH of Feijoa calli extract was 13.45 μg/mL, it was also confirmed by FRAP and ABTs values. Feijoa calli extract decreased platelet aggregation by suppression of thrombin, extended aPTT, PT, bleeding and clotting times. It was safer than warfarin medication. From these findings the authors can conclude that Feijoa had highly anticoagulant activity and the calli production achieved the goal of the enhancement of the phenolic constituent and thus their activity.

Effect of Cultivar and Nutrient Medium on In Vitro Shoot Formation on Capitulum Explants of Gerbera (Gerbera jamesonii Bolus)

The objective of this study was to examine the shoot formationcapacity of the capitulum explants of some gerbera cultivars in differentnutrient media. Immature capitulum explants of the gerbera cultivarsAmeretto, Red Bull, Ruby Red and Yanara were cultured on Pierikmedium (P) with different concentrations of benzyladenine (BA) andRadice and Marconi medium (RM). The main P and RM media were usedeither in liquid form using cotton wool as support or solidified with agar.There was a significant interaction between the different media and thegerbera cultivars in all the parameters measured. The gerbera cultivarsshowed different responses to various media compositions with regard toshoot formation on the capitulum explant.There was a general increase invitrification rate with the increase in the BA concentration in the medium.Shoots formed in liquid media showed a high vitrification rate.

Effect of BA on Bud Grafting in Grapefruit

Experiments were conducted at Shambat Research Station,Agricultural Research Corporation, Khartoum North, to test the effect ofconcentrations and application methods of benzyladenine (BA), as a pre-budding treatment, on scion bud-break and shoot elongation of grapefruit(Citrus paradisi Macf. cv. “Redblush”). Nine- to twelve- months old sourorange (Citrus aurantium L.) seedlings were used as rootstock. Twoapplication methods of BA (foliar and soaking) were evaluated. Theresults showed that increasing BA concentration increased all parametersmeasured relative to the controls, irrespective of the application method.The highest percentage of bud-break, the largest number of leaves, thelongest scion shoots and the most growth rating values were obtainedwith soaking in 400 mg BA/litre treatment, compared with the control orthe lower or higher BA concentrations tested or plants treated with thefoliar application method. No significant differences were obtainedamong BA concentrations tested or application methods used on scionshoot length. However, soak application method gave high values for allparameters measured compared with the soak application method.Treatment duration had no effect on the efficiency of BA on bud break.

Combined Effects of Cytokinin and UV-C Light on Phenolic Pattern in Ceratonia siliqua Shoot Cultures

Carob (Ceratonia siliqua L.) is an underutilized traditional crop in the Mediterranean regions that has gained much interest due to its high nutritional traits and resilience to drought and salinity. However, conventional methods of carob propagation are not enough to meet its increasing market demands. The present study analyzes to what extent benzyl adenine (BA) treatments (0.1, 0.5 and 1.0 mg L−1) alone or in combination with UV-C irradiation (3.34 and 10.01 kJ m−2) affect the antioxidant capacity and the levels of phenolic compounds in in vitro carob shoot cultures as well as their performance in terms of the content of photosynthetic pigments and sugars. Results showed that the combination of both treatments resulted in an increased content of chlorophylls, carotenoids, and sugars, particularly at 0.5 mg L−1 BA and the highest UV-C dose. Antioxidant capacity, assessed by the DPPH method, and the levels of soluble flavonoids, flavones and flavonols, and hydroxycinnamic acids were highly influenced by the interaction between BA and UV-C in the combined treatments. This indicates a clear dependence on BA concentration in the response of carob in vitro shoots to UV-C. This could be the basis for the implementation of more efficient carob micropropagation processes.

Optimization of In Vitro Propagation of Pear (Pyrus communis L.) ‘Pyrodwarf®(S)’ Rootstock

Pears are among the most economically important fruits in the world that are grown in all temperate zones. Pyrus communis L., ‘Pyrodwarf®(S)’ rootstock is one of the gene sources used to improve fruit productivity, rootstock resistance, and tolerance to biotic and abiotic stresses. Traditional propagation of P. communis L. is time-consuming and limited by a short growing season and harsh winter conditions. Therefore, in vitro propagation is a suitable alternative. Murashige and Skoog medium (MS) and woody plant medium (WPM) supplemented with different concentrations of 6-benzyladenine (BA) and kinetin (Kin), individually or in combination, were used for in vitro shoot proliferation. Nodal segments were used as explants. MS medium augmented with indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) was then used for rooting of microshoots. A combination of 2 mg·L−1 BA and 1 mg·L−1 Kin in MS medium resulted in a significant improvement in shoot proliferation. This combination produced the highest number of shoots (4.352 per explant) and leaves (10.02 per explant). The longest shoots (4.045 cm) were obtained in WPM enriched with 1 mg·L−1 BA. However, these shoots were not suitable for multiplication and rooting steps. The largest number of roots (5.50 per microshoot) was obtained on MS medium augmented with IAA at 1 mg·L−1. The produced plantlets were cultivated in pots filled with perlite and cocopeat (in a ratio of 1:3) and acclimatized gradually in a greenhouse, recording an even 90% survival rate.

Establishment of Direct Organogenesis Protocol for Arachis hypogaea cv. Virginia in Liquid Medium by Temporary Immersion System (TIS)

Peanuts (Arachis hypogaea L.) are a rich source of herbal oil, proteins, minerals, vitamins, fibers, essential amino acids, as well as bioactive compounds, and are thus widely used for human nutrition and animal feed, and for prevention from certain diseases. However, the in vitro regeneration response of the species is generally low, and it also displays a significant variability among its varieties. Thus, the development of advanced protocols and approaches for the in vitro propagation of peanut is still of immense importance. A recently developed in vitro propagation technique, TIS; Temporary Immersion Bioreactor System, provides a new approach for the mass propagation of plants. Accordingly, the present study provides an efficient de novo regeneration protocol for Arachis hypogaea L. cv. Virginia by using a TIS. Different concentrations of cytokinins, i.e., benzyladenine (BA) or thidiazuron (TDZ), were tested with several combinations of dry and medium immersion periods of TIS, corresponding to a total of 8, 12, 16, 24, 32, 36, 48, 64, 72, and 96 min daily immersions for the induction of direct organogenesis. The study exhibited that an MS medium added to 110 µM BA or 10 µM TDZ are the most appropriate medium formulations in TIS, when applied for 16 min every 16 h. The application of optimized procedures to cv. NC7 and two valuable Turkish autochthonous varieties, 7 × 77 and Com74, is also reported. To the best of our knowledge, the present study draws attention also for being the first study in which a TIS was used for peanuts.

In vitro Propagation of Curcuma caesia Roxb. via Bud Culture Technique and ISSR Profiling of the Plantlets for Genetic Homogeneity

An in vitro propagation protocol has been developed for Curcuma caesia Roxb., an endangered medicinal plant by Foundation for Revitalisation of Local Health Traditions and Central Forest Department of India. The plant bears poorly germinated seeds and produces two-storage organs: rhizomes and multiple root tubers. Only rhizomes have medicinal-economic values. They serve as propagules too, which results in a shortage of planting material. Therefore, a complete one-year production cycle of C. caesia has been standardized through in vitro propagation including explants establishment (one month), subculture cycles (seven months), rooting (one month) followed by primary hardening (one month) and secondary hardening (two months). Dormant shoot buds on rhizome served as explants for culture initiation on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyladenine (BA) and kinetin (KIN) in combination with citric acid (CA), adenine sulfate (AdS) and indole- 3-acetic acid (IAA). Maximum bud break (70%) was obtained on MS with 8 mg L-1 BA, 8 mg L-1 KIN, 100 mg L-1 CA, 200 mg L-1 AdS and 2 mg L-1 IAA (standard medium). Shoot production potential continued on this medium during the subsequent seven-month-long subculture cycle. The in vitro raised shoots rooted best on ½-strength MS containing 1 mg L-1 indole-3-butyric acid. Plantlet survival rate was >95% after acclimatization. The genetic homogeneity of plantlets with the mother plant was analyzed using Inter Simple Sequence Repeats which generated a monomorphic banding pattern to confirm the uniformity of in vitro raised plantlets of C. caesia.