Effect of Explants Type and Plant Growth Regulators on the Stimulation and Multiplication of Vegetative Branches of Trigonella foenum-graecum L. in Vitro

Abstract

The study included the use of plant tissue culture technology in the use of plant parts and different combinations in the emergence and multiplication of farms of the vegetative branches of the fenugreek plant(1,2,3)mg.l-1 BA, and the second stage was carried out by cultivating the growths resulting from the nodes grown in the previous stage on the MS medium, which was prepared with different concentrations (0, 1, 2, 3) mg.l-1 BA and (0.0,0.1,0.2,0.4)mg.L-1 NAA. The study’s results showed that the concentration of 2 mg.L-1 BA achieved a response rate of 80% compared to the peaks that did not respond. The results also showed that the same concentration of benzyl adenine achieved the highest rate in the number and length of branches, and the number of leaves reached 8.69.plant-1, 4.00 cm, 26.33 leaves.plant) respectively, compared with the control treatment that achieved the lowest rate, and the concentration 0.2 mg.l-1 NAA excelled in achieving the highest rate of the number of branches and leaves reached (7.49 branches. plant-1 and 21.50 leaves.plant-1) respectively. In comparison, the concentration of 0.4 mg.l-1 NAA excelled in achieving the highest average length of branches, reaching 3.92 cm.