In Vitro Propagation of Two Tomato Hybrids (Lycopersicon Esculentum Mill.) Via Tissue Culture Technique

Abstract

This investigation was carried out at the Tissue Culture Lab., Biotechnology Research Center (BTRC) in Tripoli, Libya in 2009 to establish a micro propagation protocol for two hybrids of Tomato in which several factors were studied including the effect of concentration of growth regulator on shoot multiplication, and rooting of explant on MS media. Shoot tips were cultured into (MS medium) with different concentrations of benzyl adenine (BA) + 0.5 mg/l (IAA) compared with MS basal medium as a control treatment. Generally, the results showed that there were no significant differences between several concentrations of (BA+IAA) on the number of leaves and shoot length. The obtained results illustrated was M1 gave high number of shoots/explant, shoot length, and leaves number for two hybrids of Tomato which were 1.90, 3.89, 4.48 respectively compared with the control. The results showed that there were no significant differences between Tomato hybrids on shoot length and the number of shoots. The best result for leaves number was hybrid HH-56 which gave 4.54 leaves. From the foregoing results, MS media supplementing IAA at 0.2 mg/l was the best treatment for rooting stage of hybrid HH-56 and Hybrid AZHAR F1. The plantlets were transferred to plastic cups containing peatmoss + sand at 1:1 (V/V). Acclimatization of plantlets were successfully.