Seminal Plasma Concentrations Research Articles

Effect of Coenzyme Q10 supplementation on antioxidant enzymes activity and oxidative stress of seminal plasma: a double-blind randomised clinical trial

Low seminal plasma concentrations of coenzyme Q10 (CoQ10) have been correlated with impaired sperm parameters, but the exact mechanism remains of dominating interest. This randomised, placebo-controlled study examined the effect of CoQ10 on catalase, superoxide dismutase (SOD) and F2 -isoprostanes in seminal plasma in infertile men and their relation with CoQ10 concentration. Sixty infertile men with idiopathic oligoasthenoteratozoospermia (OAT) were randomised to receive 200 mg d(-1) of CoQ10 or placebo for 3 months. 47 persons of them completed the study. Semen analysis, anthropometric measurements, diet and physical activity assessment were performed for subjects before and after treatment. Independent and paired t-test, chi-square test and ancova were compared outcomes of supplementation between two groups. CoQ10 levels increased from 44.74 ± 36.47 to 68.17 ± 42.41 ng ml(-1) following supplementation in CoQ10 (P < 0.001). CoQ10 group had higher catalase and SOD activity than the placebo group. There was a significant positive correlation between CoQ10 concentration and normal sperm morphology (P = 0.037), catalase (P = 0.041) and SOD (P < 0.001). Significant difference was shown between the mean of changes in seminal plasma 8-isoprostane in two groups (P = 0.003) after supplementation. Three-month supplementation with CoQ10 in OAT infertile men can attenuate oxidative stress in seminal plasma and improve semen parameters and antioxidant enzymes activity.

The detoxication of nitrate by two antioxidants or a probiotic, and the effects on blood and seminal plasma profiles and reproductive function of New Zealand White rabbit bucks

Forty-two New Zealand White male rabbits were housed individually in wire cages and randomly distributed among six experimental groups of seven rabbits each, during 16 to 61 weeks of age. There were three main nitrate groups: 0 (tap water), 350 and 700 ppm. Within the 700 ppm of nitrate, there were four subgroups, in which one group was used as control group and the other three groups were supplemented with either 200 ppm of ascorbic acid (vitamin (Vit) C), 200 ppm of Vit E with 0.2 ppm of selenium (Se) and 1000 ppm of probiotic. The nitrate was supplemented as a sodium nitrate. The aim is to test the ability of Vit C and Vit E, Se and probiotic on the deleterious effects (blood and seminal plasma biochemical constituents, semen quality and productive performance) of nitrate in drinking water. Rabbits given nitrate at 700 ppm had significantly lower plasma globulin, red blood cells (RBCs), hemoglobin (Hgb), packed cell volume % (PCV%) and total antioxidant capacity (TAC) than those given the other concentrations of nitrate. Vit C, Vit E with Se and probiotic resulted in significantly (P < 0.05) greater Hgb, RBCs, PCV% and TAC than those of bucks given water supplemented with only 700 ppm nitrate, but the aspartate aminotransferase and alanine aminotransferase concentrations in seminal plasma were lower. Testosterone in the blood plasma and the seminal plasma was significantly (P < 0.05) lower in rabbits given 700 ppm nitrate than in those given other concentrations of nitrate. Vit C, Vit E with Se and the probiotic significantly increased testosterone, fertility, number of offspring and total offspring weight of rabbits sired by bucks supplemented with 700 ppm of nitrate.

Semen of Beluga, Huso huso: Ionic Content and Osmolality of Seminal Plasma and Their Physiological Correlation with Sperm Motility Indices

The ionic contents and osmolality of seminal plasma of beluga spermatozoa to determine and their physiological relationship with sperm motility characteristics (total duration of sperm motility and percentage of motile cell) were studied. The results obtained show that Na+ (82.54 ± 5.46 mM L-1), Cl- (15.95 ± 0.72 mM L-1) and K+ (3.57 ± 0.15 mM L-1) were predominant ions in the seminal plasma. The osmolality of seminal plasma ranged from 57 to 93 mOsm kg-1. Significant positive correlations were observed between osmolality vs. Na+ (p<0.01) and Cl- (P<0.05) and Na+ vs. Cl- (P<0.05). No significant correlations were found between sperm motility and others composition of the seminal plasma. Probably, the Na+ and Cl- are the main electrolytes playing a key role in maintaining the osmolality of the seminal plasma and the viability of the spermatozoa in vivo. However analysis of ionic contents of the seminal plasma revealed some species-specific characteristics especially for K+ which is important because of its inhibitory effect on sperm motility. Results of the present study provide new data on beluga spermatozoa quality and can be utilized for successful reproduction management and cryopreservation protocols of this endangered species.

Importance of the assessment of coenzyme Q10, alpha-tocopherol and oxidative stress for the diagnosis and therapy of infertility in men

Male infertility is one of the most stressful factors of couples, being present in about 40% cases. It is usually caused by a low number of sperm (oligozoospermia) or poor sperm motility (asthenozoospermia). The sperm motility is used as an indicator of semen quality and male infertility. To the impairment of male reproduction health can contribute genetic, nutritional and environmental factors, smoking and drugs. It is well documented that excessive reactive oxygen species (ROS) production decreases sperm motility, impairs sperm function, damages the morphology of spermatozoa (1, 2). To the decreased sperm motility contribute also disturbances of sperm mitochondrial function and energy production, low levels of coenzyme Q10 and carnitine, as well as sperm mitochondrial deoxyribonucleic acid (DNA) defects. The origin of sperm dysfunction, however, is not well understood. Oxidative stress has been established as a major factor in the pathogenesis of male infertility. Low level of coenzyme Q10 contributes to the decreased sperm motility, which plays a vital role in sperm mitochondrial energy production and neutralization of reactive oxygen species (ROS).The aim of the present study was to find out, if an assessment of coenzyme Q10-TOTAL (CoQ10-TOTAL), α-tocopherol, γ-tocopherol and oxidative stress could contribute to the diagnosis of infertility in men. Two groups of infertile men, according to sperm motility (a+b and b+c) were included in the study. CoQ10-TOTAL, α-tocopherol, γ-tocopherol in plasma and seminal fluid, and parameter of oxidative stress (thiobarbituric acid reactive substances - TBARS) in plasma were determined. Higher sperm density and decreased sperm pathology were found in group a+b vs b+c (class a and b - fast and weak forward motility, class c - nonprogressive motility). Concentrations of CoQ10-TOTAL and α-tocopherol were significantly increased in seminal fluid of groups a+b vs b+c, opposite results were estimated in plasma. Concentrations of γ-tocopherol in plasma and seminal fluid of both groups were similar. Plasmatic TBARS concentrations were increased in both groups of infertile men. We suppose that incorporation of oxidative stress assessment, CoQ10-TOTAL and α-tocopherol concentrations in seminal fluid and plasma into routine andrology can play an important role for the diagnosis and targeted therapy of male infertility (Tab. 1, Ref. 16).

Prostatic acid phosphatase in serum and semen of dogs

The incidence of prostatic malignancy has increased the use of tissue markers to detect cancer. Tissue specific antigens or differentiation antigens are found on the surface of normal cells. Clinically, these antigens are important to diagnose alterations in the tissues and for immunotherapy. The objective of the present study was to evaluate the prostatic acid phosphatase concentration in blood and seminal plasma of intact and healthy dogs at different ages. The evaluation was carried out by spectrophotometer, using a commercial kit. The prostatic acid phosphatase (PAP) levels did not differ according to the age and did not correlate with age or prostatic dimensions verified by ultrasonography. The PAP concentration values varied greatly within each group. However, more studies are necessary to evaluate the role of prostatic acid phosphatase in the canine prostate and its importance as a diagnostic test for prostate disorders.

Evaluation of lipid peroxidation in cases of idiopathic male infertility: correlation with the hypo-osmotic swelling test

Background Lipid peroxidation of sperm plasma membranes has a deleterious effect on the semen quality, and measurement of malondialdehyde acid (MDA) is the most widely used method for assessing lipid peroxidation. High levels of seminal MDA represent increased lipid peroxidation rates and oxidative damage to the sperm plasma membranes, which may result in the impairment of the integrity and functioning of sperm plasma membranes and damage of both nuclear and mitochondrial DNA. Aim of the study The aim of the present study was to investigate the seminal plasma MDA concentration as a marker of lipid peroxidation and its correlation with the hypo-osmotic swelling test (HOST) scores as a predictor for the functional integrity of the sperm membrane in cases of idiopathic male infertility. Participants and methods A total of 93 men were included in this study: 68 men who had been attending to our andrology outpatient clinics with idiopathic infertility were selected as cases and 25 healthy fertile men were assigned as control group. Both groups were subjected to the following laboratory investigations: semen analysis, peroxidase test, HOST, and measurement of MDA in the seminal plasma. Results Our results showed that seminal plasma concentration of MDA was significantly higher in men with idiopathic infertility compared with the fertile control group ( P ≤ 0.001), and HOST was significantly lower in men with idiopathic infertility compared with the fertile control group ( P ≤ 0.001). In addition, the results showed a strong negative correlation between MDA concentration and HOST ( P ≤ 0.001). Conclusion Our results suggest that the evaluation of lipid peroxidation must be used in combination with the HOST as a diagnostic tool in men with idiopathic infertility in addition to standard semen analysis.

Seminal plasma effects on sex-sorting bovine sperm

The objective was to determine which characteristics of bovine ejaculates affected efficacy of sex sorting bovine sperm by flow cytometry. The effects of first versus second ejaculates, seminal plasma content, addition of BSA, and seminal plasma from different bulls during staining were all studied, as was the effect of 8-hour storage with and without seminal plasma. Semen collected by artificial vagina was centrifuged at 1000 ×g for 15 minutes to separate sperm from seminal plasma; seminal plasma was clarified by 10 minutes of additional centrifugation at 2000 ×g. Sperm were rediluted to 160 × 106 sperm per mL with: Tyrode's medium plus albumin, lactate, and pyruvate (TALP) containing 0%, 5%, 10%, or 20% homologous seminal plasma, TALP containing 10% heterologous seminal plasma, or TALP containing 0.3% (control), 0.6%, or 1.2% BSA. After incubation with Hoechst 33342 for 45 minutes, an equal volume of TALP containing red food dye was added, and sperm were analyzed by flow cytometry/cell sorting to determine percent of live-oriented sperm, X sort rate, percent of membrane-impaired sperm, and split (degree of separation between X- and Y-bearing sperm populations). The percent of live-oriented sperm was higher for sperm incubated with 0% seminal plasma (64%) than for sperm incubated with 5%, 10%, or 20% seminal plasma (60%, 59%, and 58%, respectively; P < 0.05). The X sort rate was higher for sperm incubated with 0% seminal plasma than sperm with 20% seminal plasma (4.26 vs. 3.61 × 103 sperm per second). When seminal plasma was exchanged between bull ejaculates, only one bull had seminal plasma that was detrimental to sperm, resulting in 31% membrane-impaired sperm compared with a range of 16% to 19% for seminal plasmas from other bulls (P < 0.05). The addition of BSA did not affect sort efficiency at the concentrations studied. Sperm from six bulls stored for 8 hours without seminal plasma had more membrane-impaired sperm (which were discarded) during sorting (28%) than with seminal plasma (19%; P < 0.01), but higher postthaw motility postsorting (63%) than with seminal plasma (52%; P < 0.05). In conclusion, the presence of seminal plasma during staining and sorting decreased sort rates and percent of live-oriented sperm, and storing sperm without seminal plasma increased postthaw motility.

Detection of haptoglobin in seminal plasma of Awassi rams and the relation with its level in serum and some semen parameters

The study was conducted to detect haptoglobin in seminal plasma (SP-Hp) of Awassi rams and the effect of the breeding season on its concentration, along with determining the correlation with its concentration in serum (S-Hp) and main semen variables. Pre-warmed artificial vagina was used to collect semen samples biweekly from five Awassi rams. Semen samples were evaluated for volume, concentration and sperm motility. Blood samples were collected 10–30min after semen collection. The concentration of serum and seminal plasma Hp was determined using ELISA. The mean±SESP-Hp concentrations ranged from 0.25±0.05 to 0.81±0.44μg/ml, whereas those of S-Hp-ranged from 0.99±0.29 to 2.99±0.18mg/ml. There was a significant (P<0.05) positive correlation (r=0.329) between SP-Hp and S-Hp concentrations. Both SP-Hp and S-Hp concentrations were significantly (P<0.05) higher in winter as compared with the other seasons. The concentrations of SP-Hp and S-Hp during the breeding season were significantly lower (P<0.01) than those of the out season period. SP-Hp concentration negatively correlated with semen volume and sperm concentration (r=−0.164 and −0.121), whereas sperm concentration positively correlated with individual sperm motility (r=0.100). No significant correlation was detected between SP-Hp and semen parameters. It can be concluded that, Hp is present in ram seminal plasma and its concentration was about 2000 folds lower than that of the serum, and Hp concentration was lower during the breeding season, but its concentration in seminal plasma has no significant correlation with semen parameters.

Spermatozoa motility and variation in the seminal plasma proteome of Eurasian perch (Perca fluviatilis) during the reproductive season

This study evaluated physiological and functional sperm parameters and the seminal plasma proteome of Eurasian perch (Perca fluviatilis) over the course of their reproductive season. Spermatozoa velocity (169.56 ± 6.53 to 158.5 ± 7.4 µm sec(-1)), percent motility (95.89 ± 4.28% to 89.55 ± 4.5%), and osmolality of seminal plasma (290 ± 5 to 297 ± 12 mOsmol kg(-1)) remained stable throughout the reproductive season. Milt volume and protein concentration of seminal plasma gradually increased and reached the highest values late in the reproductive period. Spermatozoa concentration peaked in the mid-reproductive season (66.90 ± 13 × 10(9) spermatozoa ml(-1)) and decreased towards the end (54 ± 10 × 10(9) spermatozoa ml(-1)). A proteomic analysis of seminal plasma using two-dimensional polyacrylamide gel electrophoresis revealed 10 protein spots significantly altered over the course of the reproductive season. Subsequent protein characterization suggested that time in the reproductive season predominantly affected proteins involved in membrane trafficking, organization, cell motility, and oxido-reductase activity. This study provides new data on physiological properties of sperm and protein patterns of seminal plasma over the course of the reproductive season that should be considered in the development of methods for artificial reproduction of perch.

Lead level in seminal plasma may affect semen quality for men without occupational exposure to lead

BackgroundInfertility affects approximately 10–15% of reproductive-age couples. Poor semen quality contributes to about 25% of infertile cases. Resulting from the direct effect on testicular function or hormonal alterations, heavy metals exposure has been related to impaired semen quality. The objective of this study was to assess the level of lead in the seminal plasma in men without occupational exposure to lead, and to determine the relationship between semen quality and lead concentration in the semen.MethodsThis is a prospective and nonrandomized clinical study conducted in University infertility clinic and academic research laboratory. Three hundred and forty-one male partners of infertile couples undergoing infertility evaluation and management were recruited to the study. Semen samples collected for the analyses of semen quality were also used for the measurement of lead concentrations. Semen samples were evaluated according to the WHO standards.ResultsAll subjects were married and from infertile couples without occupational exposure to lead. There is a significant inverse correlation between the lead concentration in seminal plasma and sperm count. A higher semen lead concentration was correlated with lower sperm count, but not with semen volume, sperm motility or sperm morphology as assessed by simple linear regression.ConclusionsWe found that semen lead concentration was significantly higher among the patients with lower sperm count. To our knowledge, this is the first study to demonstrate that a high level of lead accumulation in semen may reduce the sperm count contributing to infertility of men without occupational exposure to lead.

Etravirine concentrations in seminal plasma in HIV‐infected patients

Purpose of the studyGood penetration of antiretroviral drugs to the seminal plasma may be associated with a decrease in viral replication and play an important role in the prevention of sexual transmission of HIV. We present data from a series of HIV‐infected ARV‐experienced patients receiving etravirine‐containing regimens, in whom etravirine concentrations and viral loads were determined in blood plasma and seminal plasma. The objective was to determine etravirine concentrations and HIV‐1 viral load (VL) in blood plasma (BP) and seminal plasma (SP) of HIV‐infected patients.MethodsTen HIV‐1 adult antiretroviral‐experienced patients receiving an etravirine‐containing regimen for at least 1 month were enrolled. Semen and blood samples were both collected around 12–24 h after the last etravirine dose, depending on once‐daily or twice‐daily dosing, respectively. HPLC/MS/MS was used to determine etravirine concentrations, and HIV‐1 VL was determined by real‐time PCR (limit of detection, VL 40 copies/mL).ResultsTen blood and twenty semen samples were collected. Median (range) CD4 count was 502 cells/mm3 (252–817) and median (range) BP VL was&lt;40 copies/mL (40–362). Median (range) time on etravirine was 52 weeks (12–124). Median (range) BP etravirine concentration was 452.5 ng/mL (258–751). Median (range) SP etravirine concentration was 62.9 ng/mL (31.2–166), and values were above the protein‐free IC50 range (0.39–2.4 ng/mL) in all cases. Median (range) etravirine SP:BP ratio was 0.16 (0.07–0.26). SP VL was&lt;40 copies/mL in all patients, whereas BP VL was detectable in one patient with poor adherence to treatment. Patient SP (ng/ml) BP (ng/ml) SP VL (copies/ml) BP VL (copies/ml) SP:BP Concomitant ARV 1 31.2 375 &lt;40 &lt;40 0.08 DRVr/TDF 2* 57.1 307 &lt;40 &lt;40 0.18 3TC/ABC 3* 36.9 506 &lt;40 &lt;40 0.07 TDF/FTC 4* 60.6 399 &lt;40 &lt;40 0.15 3TC/ABC 5 68.1 258 &lt;40 &lt;40 0.26 LPVr/TDF 6* 104 414 &lt;40 &lt;40 0.25 3TC/ABC 7 107 491 &lt;40 362 0.21 DRVr/TDF/FTC 8* 65.3 592 &lt;40 &lt;40 0.11 TDF/FTC 9 52.2 518 &lt;40 &lt;40 0.1 DRVr 10 166 751 &lt;40 &lt;40 0.22 TDF/FTC Median 62.9 452.5 &lt;40 &lt;40 0.16 Patients taking ETR once a day. ConclusionsTotal etravirine concentrations in male genital secretion are modest, reaching only 16% of the BP concentration, but nevertheless, more than 10 times above the wild type IC50 range.

Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters

This study was designed to test a vitrification method on spermatozoa of sex-reversed rainbow trout and determine the ability of sucrose, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA) and seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium comprised a standard buffer for fish spermatozoa (Cortland®medium)+10% DMSO+2% BSA+0.13M sucrose+SP at concentrations of 30% (group 1), 40% (group 2) or 50% (group 3). Fresh sperm (F) was used as a control. For cooling, 30μL suspensions of spermatozoa from each group were dropped directly into liquid nitrogen. After cooling, the spheres were placed in cryotubes for storage in liquid nitrogen. For thawing, cryotubes with the vitrified spermatozoa were placed in a water bath at 37°C for 60s. After warming, the following sperm quality parameters were determined by flow cytometry: viability and plasma membrane integrity (SYBR-14/PI, staining technique); mitochondrial membrane integrity (JC-1 staining); and DNA fragmentation (TUNEL). Sperm function was assessed by fertility trials. Spermatozoa quality variables were best preserved when the highest SP concentration (50%) was used (viability 97.3%, plasma membrane integrity 98.4%, mitochondrial membrane integrity 36.2%, DNA fragmentation 11.1%). Similarly, fertilization rates were higher with sperm that were vitrified in medium with 50% SP. This is the first report on the physiological parameters of the cryopreservation of spermatozoa from sex-reversed rainbow trout (Oncorhynchus mykiss) by direct plunging into liquid nitrogen (vitrification).

Boar seminal plasma components and their relation with semen quality

Select boar seminal plasma (SP) components and their relation to semen quality were investigated. Thirty nine boars from three artificial insemination (AI) centers were divided into group A (GA: > 80% normal sperm and >70% motility) and group B (GB: < 80% normal sperm and < 70% motility). Each ejaculate was collected and semen volume, concentration, sperm motility (computer aided semen analysis; CASA), morphology, and vitality (both eosin nigrosin staining) were investigated. The SP was separated and analyzed for aspartate-amino-transferase (AST), γ-glutamyl-transferase (GGT), alkaline phosphatase (ALP) activity, and the concentrations of sodium (Na), potassium (K), chloride (Cl), calcium (Ca), phosphate (PO43-), magnesium (Mg), selenium (Se) and zinc (Zn) were assessed. Repeated measures (2 months interval) were conducted in eight boars of GA from one AI center. The activity of GGT (r = -0.482) and ALP (r = -0.459) was moderately associated (p < 0.05) with ejaculate volume and strongly associated with concentration (r = 0.580 and r = 0.618, respectively; p = 0.000). Moderate associations (p < 0.05) were found between ALP (r = 0.439), GGT (r = 0.387), Na (r = -0.428), K (r = 0.354), and Se (r = 0.354) with progressive motility. The SP concentration of Na (r = -0.401), Cl (r = -0.521), and K (r = 0.350) was associated (p < 0.05) with normal morphology. Only Mg was associated (p < 0.05) with membrane damage (r = -0.335). The concentration of Na, Cl, and Zn (1681.0 vs. 1701.0 µg/dL) was different between groups (p < 0.05). Repeated measures showed significant differences in time but only for Na, Mg, and Zn (p < 0.05). In conclusion, several biochemical components of SP were related to semen quality. The analysis of biochemical parameters could provide extra information about reproductive health of AI boars.

Etravirine concentrations in seminal plasma in HIV-infected patients

To determine etravirine concentrations and the HIV-1 viral load (VL) in blood plasma (BP) and seminal plasma (SP) of HIV-infected patients. Ten adult antiretroviral-experienced HIV-1 patients receiving an etravirine-containing regimen for at least 1 month were enrolled. Semen and blood samples were collected ~12 or 24 h after the last etravirine dose, depending on twice-daily or once-daily dosing, respectively. Liquid chromatography tandem mass spectrometry was used to determine etravirine concentrations and HIV-1 VL was determined by real-time PCR (detection limit 40 copies/mL). Results are presented as the median (range) unless otherwise indicated. Ten blood and 20 semen samples were collected. The CD4 count was 502 (252-817) cells/mm(3) and the BP VL was <40 (<40-362) copies/mL. The time on etravirine was 52 (12-124) weeks. The BP etravirine concentration was 452.5 (258-751) ng/mL. The SP etravirine concentration was 62.9 (31.2-166.0) ng/mL and values were above the IC(50) range (0.39-2.4 ng/mL) in all cases. The median etravirine SP:BP ratio was 0.16 (0.07-0.26). The SP VL was <40 copies/mL in all patients, whereas the BP VL was detectable in one patient with poor adherence to treatment. Etravirine concentrations in male genital secretions are modest, reaching only 16% of the BP concentration. Nevertheless, they are more than 10 times greater than the wild-type IC(50) range (not adjusted for protein binding).

Quantification studies in human seminal plasma samples identify prolactin inducible protein as a plausible marker of azoospermia

Background: Prolactin inducible protein (PIP) is a ~17 kDa protein, which is known to play vital roles in immunoregulation, fertility, antimicrobial activity, apoptosis and tumour progression.Objectives: This study reports quantification of PIP concentration in human seminal plasma (SP) samples.Methodology: PIP was purified by immunoprecipitation and its concentration in human SP samples was quantified by ELISA method.Results: Average concentration of PIP in normozoospermia, oligozoospermia and azoospermia was 290.3 ± 71.5 µg/mL, 306.4 ± 71.2 µg/mL and 60.5 ± 23.6 µg/mL respectively.Conclusion: There was no significant variation in PIP levels in normozoospermia and oligozoospermia while its expression was down-regulated in azoospermia, indicating that PIP may be a plausible marker of azoospermia.

Correlation between cytokine concentrations in seminal plasma of fertile and subfertile patients and sperm parameters and their effect on ICSI outcome

Correlation between cytokine concentrations in seminal plasma of fertile and subfertile patients and sperm parameters and their effect on ICSI outcome

Identificação do fator de crescimento semelhante à insulina i no plasma seminal de varrões e sua influência na qualidade espermática

A presente pesquisa foi realizada para investigar as relações entre os parâmetros dos espermatozóides e as concentrações de IGF-I no plasma seminal de varrões. Amostras de sêmen (duplicatas) foram coletadas de machos maduros (n=27). Em cada ejaculação, as características macroscópicas e microscópicas foram determinadas. Após a centrifugação para separar espermatozóides do plasma seminal, as concentrações de IGF-I em amostras de plasma seminal foram determinadas por kit de imunoensaio humano específico. A concentração média de IGF-I do plasma seminal de varrões foi de 1,5±0,2 ng/mL (n=54 amostras). Não houve correlação (p&gt;0,05) entre IGF-I com a maioria dos parâmetros avaliados. Houve apenas uma correlação baixa negativa (-0,29, p=0,0331) entre a concentração de IGF-I no plasma seminal e a taxa de degradação da motilidade. Este estudo sugere que o IGF-I no plasma seminal de varrões não está relacionado com os parâmetros seminais iniciais do sêmen in natura. No entanto, o hormônio aumentou a duração da motilidade espermática.

Rapid measurement of 8-oxo-7,8-dihydro-2′-deoxyguanosine in human biological matrices using ultra-high-performance liquid chromatography–tandem mass spectrometry

Interaction of reactive oxygen species with DNA results in a variety of modifications, including 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), which has been extensively studied as a biomarker of oxidative stress. Oxidative stress is implicated in a number of pathophysiological processes relevant to obstetrics and gynecology; however, there is a lack of understanding as to the precise role of oxidative stress in these processes. We aimed to develop a rapid, validated assay for the accurate quantification of 8-oxodG in human urine using solid-phase extraction and ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) and then investigate the levels of 8-oxodG in several fluids of interest to obstetrics and gynecology. Using UHPLC–MS/MS, 8-oxodG eluted after 3.94min with an RSD for 15 injections of 0.07%. The method was linear between 0.95 and 95nmol/L with LOD and LOQ of 5 and 25fmol on-column, respectively. Accuracy and precision were 98.7–101.0 and <10%, respectively, over three concentrations of 8-oxodG. Recovery from urine was 88% with intra- and interday variations of 4.0 and 10.2%, respectively. LOQ from urine was 0.9pmol/ml. Rank order from the greatest to lowest 8-oxodG concentration was urine>seminal plasma>amniotic fluid>plasma>serum>peritoneal fluid, and it was not detected in saliva. Urine concentrations normalized to creatinine (n=15) ranged between 0.55 and 1.95pmol/μmol creatinine. We describe, for the first time, 8-oxodG concentrations in human seminal plasma, peritoneal fluid, amniotic fluid, and breast milk, as well as in urine, plasma, and serum, using a rapid UHPLC–MS/MS method that will further facilitate biomonitoring of oxidative stress.

1089 HEAT-SHOCK PROTEIN 70 EXPRESSION IN THE SEMINAL PLASMA OF PATIENTS WITH CHRONIC BACTERIAL PROSTATITIS AND CHRONIC PROSTATITIS/CHRONIC PELVIC PAIN SYNDROME

You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Prostate & Genitalia1 Apr 20121089 HEAT-SHOCK PROTEIN 70 EXPRESSION IN THE SEMINAL PLASMA OF PATIENTS WITH CHRONIC BACTERIAL PROSTATITIS AND CHRONIC PROSTATITIS/CHRONIC PELVIC PAIN SYNDROME hui guo, yuemin xu, and zhangqun ye hui guohui guo shanghai, China, People's Republic of More articles by this author , yuemin xuyuemin xu shanghai, China, People's Republic of More articles by this author , and zhangqun yezhangqun ye wuhan, China, People's Republic of More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1196AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The purpose of this study was to assess the diagnostic value and potentially protective capacity of heat-shock protein 70 (HSP70) in chronic bacterial prostatitis and chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS). METHODS In this study, seminal plasma levels of cytokines (tumor necrosis factor-alpha (TNF-alpha) and interleukin 1beta (IL-1beta)) and HSP70 were evaluated by enzyme-linked immunosorbent assay in 80 men: 15 healthy controls, 16 men with chronic bacterial prostatitis, 23 men with CP/CPPS IIIA and 26 with CP/CPPS IIIB. The HSP70 levels in chronic bacterial prostatitis and CP/CPPS patients were correlated with chronic prostatitis symptom index (CPSI). RESULTS Significantly increased levels of cytokines (TNF-alpha and IL-1beta) and HSP70 were observed in seminal plasmas from patients with chronic bacterial prostatitis compared with CP/CPPS patients and controls. However, only IL-1beta was significantly elevated compared with CP/CPPS IIIB and controls in patients with CP/CPPS IIIA. HSP70 levels in CP/CPPS patients were significantly lower than that in controls. HSP70 concentration in seminal plasma was negatively correlated with CPSI in chronic bacterial prostatitis. Whereas,the expression of HSP70 did not correlate with the NIH-CPSI total scores in CP/CPPS patients. CONCLUSIONS In conclusion, HSP70 and IL-1b are measurable in human semen plasma and they provide useful information in diagnosing prostatic inflammatory conditions and categorizing CPPS III subclasses, respectively. Levels of HSP70 expression are feasible to regard as diagnosis and prognostic indicators for chronic bacterial prostatitis. HSP70 levels may be suggested to assess the severity of prostatic inflammation in these patients.IL-1b appears to represent a useful biomarker in CP/CPPS and can be used to discriminate CP/CPPS IIIA from IIIB. HSP70 provided cellular protection capacity that has pivotal roles in the regulation of cell functions in chronic bacterial prostatitis. CP/CPPS may disrupt the ability of T cells to suppress the immune system and consequently decrease HSP70 expression in these patients.It is further proved that the CP/CPPS is a kind of immune disease. Autoimmune may have a prominent role in the induction and progression of CP/CPPS. It should be novel treatments for chronic bacterial prostatitis and CP/CPPS and merit further study by enhancing HSP70 expression and exogenous HSP70 administration. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e442 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information hui guo shanghai, China, People's Republic of More articles by this author yuemin xu shanghai, China, People's Republic of More articles by this author zhangqun ye wuhan, China, People's Republic of More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...

Leptin and insulin in the seminal plasma of zebu bulls in peripuberty

In order to determine the profile of leptin and insulin values in seminal plasma and their correlations with sperm patterns during the peripuberty, a total of 16 crossbred Gyr dairy bulls were monitored from 60 days before to 60 days after puberty, separated into two groups, early and regular, according to the onset of puberty. Hormone values were determined by radioimmunoassay after gel filtration in fast performance liquid chromatography and semen patterns, according to the Brazilian College of Animal Reproduction. Insulin concentrations in seminal plasma did not differ between groups, nor between the ages, with the pooling of the data showing average of 35.15±16.93, 36.60±26.21, 26.82±09.81, and 43.56±31.71 32.24±16.71 U/mL for the pubertal period of -60, -30, 0, +30 and +60 days, respectively. These values were not correlated with sperm parameters, but differed in the group of animals with the highest percentage of major defects (31.08±18.58 U/mL) compared with those with lower percentage of these sperm defects (40.01±25.37 U/mL). Leptin was not correlated with sperm parameters and did not differ between groups or stage of sexual development, with concentrations of 23.10±10.43, 24.35±9.42, 22.41±9.90, 23.76±9.69 and 24.99±11.42 ng/mL for the periods of puberty aforementioned, respectively, after being grouped. These results demonstrated the binding of insulin to the structural quality of the sperm cell, and this hormone is indicated in andrologycal evaluation of future breeding soundness.