Detection of haptoglobin in seminal plasma of Awassi rams and the relation with its level in serum and some semen parameters

Abstract

The study was conducted to detect haptoglobin in seminal plasma (SP-Hp) of Awassi rams and the effect of the breeding season on its concentration, along with determining the correlation with its concentration in serum (S-Hp) and main semen variables. Pre-warmed artificial vagina was used to collect semen samples biweekly from five Awassi rams. Semen samples were evaluated for volume, concentration and sperm motility. Blood samples were collected 10–30min after semen collection. The concentration of serum and seminal plasma Hp was determined using ELISA. The mean±SESP-Hp concentrations ranged from 0.25±0.05 to 0.81±0.44μg/ml, whereas those of S-Hp-ranged from 0.99±0.29 to 2.99±0.18mg/ml. There was a significant (P<0.05) positive correlation (r=0.329) between SP-Hp and S-Hp concentrations. Both SP-Hp and S-Hp concentrations were significantly (P<0.05) higher in winter as compared with the other seasons. The concentrations of SP-Hp and S-Hp during the breeding season were significantly lower (P<0.01) than those of the out season period. SP-Hp concentration negatively correlated with semen volume and sperm concentration (r=−0.164 and −0.121), whereas sperm concentration positively correlated with individual sperm motility (r=0.100). No significant correlation was detected between SP-Hp and semen parameters. It can be concluded that, Hp is present in ram seminal plasma and its concentration was about 2000 folds lower than that of the serum, and Hp concentration was lower during the breeding season, but its concentration in seminal plasma has no significant correlation with semen parameters.