Abstract
In order to determine the profile of leptin and insulin values in seminal plasma and their correlations with sperm patterns during the peripuberty, a total of 16 crossbred Gyr dairy bulls were monitored from 60 days before to 60 days after puberty, separated into two groups, early and regular, according to the onset of puberty. Hormone values were determined by radioimmunoassay after gel filtration in fast performance liquid chromatography and semen patterns, according to the Brazilian College of Animal Reproduction. Insulin concentrations in seminal plasma did not differ between groups, nor between the ages, with the pooling of the data showing average of 35.15±16.93, 36.60±26.21, 26.82±09.81, and 43.56±31.71 32.24±16.71 U/mL for the pubertal period of -60, -30, 0, +30 and +60 days, respectively. These values were not correlated with sperm parameters, but differed in the group of animals with the highest percentage of major defects (31.08±18.58 U/mL) compared with those with lower percentage of these sperm defects (40.01±25.37 U/mL). Leptin was not correlated with sperm parameters and did not differ between groups or stage of sexual development, with concentrations of 23.10±10.43, 24.35±9.42, 22.41±9.90, 23.76±9.69 and 24.99±11.42 ng/mL for the periods of puberty aforementioned, respectively, after being grouped. These results demonstrated the binding of insulin to the structural quality of the sperm cell, and this hormone is indicated in andrologycal evaluation of future breeding soundness.
Highlights
Seminal plasma is a complex mixture of secretions originated in the testes, epididymis and accessory sex glands (Manjunath et al, 1993), serving as a vehicle and containing factors that influence the fertilization capability of the ejaculated spermatozoids (Calvete et al, 1996)
It has been shown that this hormone can act at different levels on the hypothalamic-pituitary-testicular axis, and the sperm cell itself (Nikbakh et al, 2010), and can influence the mechanisms involved in the development of motility sperm and the acrosome reaction (Jorsaraei et al, 2008)
Hicks et al (1973), working with non-diabetic men found lower seminal plasma concentrations than those reported by Póvoa Júnior et al (1973) or in the present study, with values of 19±3 μU/mL, representing more than twice the values for human serum concentration (7.5±1.5), according to Hicks et al (1973)
Summary
Seminal plasma is a complex mixture of secretions originated in the testes, epididymis and accessory sex glands (Manjunath et al, 1993), serving as a vehicle and containing factors that influence the fertilization capability of the ejaculated spermatozoids (Calvete et al, 1996). It has been shown that this hormone can act at different levels on the hypothalamic-pituitary-testicular axis, and the sperm cell itself (Nikbakh et al, 2010), and can influence the mechanisms involved in the development of motility sperm and the acrosome reaction (Jorsaraei et al, 2008). Another hormone that plays a central role in the regulation of gonadal function and quality of seminal plasma is insulin. Insulin has the plasmatic membrane of the sperm and its acrosome as cytological targets (Silvestroni et al, 1992), which may affect fertilization by influencing the motility, the acrossomal status or the metabolism of the sperm cell (Shrivastav et al, 1989; Silvestroni et al, 1992)
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