Objective To investigate the differences of influencing factors of disqualification blood between individuals and groups blood donors in Dongying City of Shandong Province, in order to ensure the safety of blood donors and blood. Methods From January 2012 to December 2015, a total of 91 534 cases of voluntary blood donors in Dongying center blood station were included in this study. According to different organization modes of blood donors, they were divided into individual donors group (n=66 012) and groups donors group (n=25 522). In accordance with relevant provisions of Regulations for Technical Operations of Blood Station (2012 and 2015 edition), Quality Management Standards of Blood Station (2006 edition), and Quality Management Standards of Blood Station Laboratory (2006 edition), 5 projects of blood examination factors were detected, which included alanine aminotransferase (ALT), hepatitis B surface antigen (HBsAg), anti-hepatitis C virus (HCV), anti-Treponema pallidum (TP) and anti-human immunodeficiency virus (HIV). In accordance with relevant provisions of Quality Requirements for Whole Blood and Blood Components (GB 18469-2012) and International Society of Blood Transfusion (ISBT), 3 projects of non-blood examination factors, which including donation reaction, chylemia and insufficient blood collection were investigate. Furthermore, Chi-square test and trend Chi-square test were used to compare the differences of disqualification rates of each item in both groups. Results ① The disqualification rates of ALT, HBsAg and anti-HCV, anti-TP, anti-HIV, donation reaction, chylemia and insufficient blood collection of blood from donors in Dongying were basic stability from 2012 to 2015, and there were no significant differences (χfrend2=0.25, -0.14, -0.12, 0.09, 0.13, 0.41, 0.83, 0.28; P>0.05). Among 5 projects of blood examination factors, the disqualification rate of ALT was the highest, amount for 3.31% (3 032/91 534), and the difference was statistically significant (χ2=127 560.72, P<0.05). Among 3 projects of non-blood examination factors, the disqualification rate of chylemia was the highest, amount for 6.52%(5 969/91 534), and the difference was statistically significant (χ2=169 443.63, P<0.05). ②The total disqualification rate of projects of blood examination factors in individual donors group was lower than that of groups donors group, and the difference was statistically significant (3.90% vs 5.70%, χ2=138.20, P<0.05). Comparison of disqualification rates of ALT, HBsAg and anti-HCV, anti-TP, anti-HIV between individual donors group and groups donors group, the differences were statistically significant (χ2=345.42, 31.31, 24.55, 14.22, 4.01; P<0.05). ③The total disqualification rate of projects of non-blood examination factors in individual donors group was lower than that of groups donors group, and the difference was statistically significant (9.26% vs 15.9%, χ2=828.72, P<0.05). Comparison of disqualification rates of donation reaction, chylemia and insufficient blood collection between individual donors group and groups donors group, the differences were statistically significant (χ2=162.47, 603.23, 828.72; P<0.05). Conclusions For individual blood donors, the health consultation of them should be strengthened before donation, and the staff of blood station ought to collect safer blood from low-risk populations. For group blood donors, authors recommend performing blood collection by reducing the number of blood donors, or increasing the quantities of bloodmobiles and staff. Through eliminating overly centralized collection, the amount of donation reactions and blood deficiency could be reduced. In addition, matters-needing-attention propaganda before group donation should be strengthened, in order to reduce the disqualification rates of chylemia and ALT. Key words: Blood donation; Blood donors; Blood safety; Individual blood donation; Group blood donation