Dynamic Monitoring on the Bacterial Endotoxin Concentration in Whole Blood and Blood Component

Abstract

Objective To explore the importance of dynamic monitoring on the bacterial endotoxin and assess the blood safety to guarantee the safety of blood transfusion,through dynamically monitoring the bacterial endotoxin concentration in whole blood and blood component. Methods From March to April 2009,300 donors' samples,which had met the requirements of donors' health check,were included in this study.According to the blood component,all these samples were performed into three groups:whole blood group (n=50),red blood cells (RBC) suspension group (n=150),platelet (PLT) components group (n=100).Dynamic turbidity in blood which were respectively stored at early stage,metaphase and later stage,were used as the detection quantitative method of bacterial endotoxin.And the results were further analyzed by variance of repeated measurement data. Results There was no difference of the bacterial endotoxin concentration during the preservation in PLT suspension and RBC suspension(P＞0.05).Meanwhile,there was significant difference of the bacterial endotoxin concentration between preservation periods of the whole blood(P＜0.05).Conclusions Through dynamically monitoring the bacterial endotoxin concentration in whole blood and blood component,the stability of bacterial endotoxin concentration during the preservation period coincides with a negative result of the bacterial culture.It could be shown that the quality control of the blood collection and supply procedure is effective to guarantee the safety of blood. Key words: whole blood; blood component; bacterial endotoxin; monitoring