Quality evaluation of red blood cell suspension and platelet concentrate separated and prepared from whole blood stored at 22℃ for 24 hours

Abstract

Objective To evaluate the quality of red blood cell suspension and platelet concentrate separated and prepared from whole blood stored at 22℃ for 24 h. Methods From May 2014 to February 2015, a total of 60 voluntary blood donors who were recruited by Maoming Central Blood Station of Guangdong Province were included into this study, using simple random sampling method. The inclusion criterion for subjects was that the physical examination and related hematology tests results of all blood donors before blood donation met the relevant regulations in the Whole Blood and Blood Component Donor Selection Requirements (GB18467-2011). All the 60 donors were randomly divided into study group (n=30) and control group (n=30). A total of 60 bags of whole blood (400 mL) were collected from the blood donors in the two groups, using quintuple blood collection bags. In the study group, the whole blood was stored and transported at 22 ℃, and prepared into red blood cell suspension and platelet concentrate 24 h after blood collection. In the control group, the whole blood was also stored and transported at 22 ℃, and prepared into red blood cell suspension and platelet concentrate within 8 h after blood collection. The preparation of red blood cell suspension and platelet concentrate in both groups was conducted by the buffy coat method. Red blood cell suspension was stored at 4 ℃ until the end of the storage period (35 d after preparation), and its sterility test was using Bact/ALERT full-automatic microbial detection system. Moreover, red blood cell count, hematocrit (HCT), hemoglobin (Hb) level, free hemoglobin (FHb) level, hemolytic ratio at the end of the storage period, and K+ , Na+ and Cl- concentrations in red blood cell suspension were compared between the study group and control group. Also, platelet concentrate was stored at 22 ℃ until the end of the storage period (5 d after preparation), and its sterilty test was using Bact/ALERT full-automatic microbial detection system. Additionally, platelet content, red cell volume, FHb level, pH value at the end of the storage period, adhesion rate, aggregation rate, and K+ , Na+ and Cl- concentrations in platelet concentrate were compared between the study group and control group. Results ① In the study group and the control group, there was no bacterial growth found in red blood cell suspension 35 d after storage, and in platelet concentrate 5 d after storage. ② In the red blood cell suspension of both groups 35 d after storage, all test results of HCT, Hb level and hemolytic ratio at the end of the storage period met the relevant requirements in Quality Requirements for Whole Blood and Blood Components (GB18469-2012). There were no significant differences comparisons of red blood cell count, HCT, level of Hb, level of FHb, hemolytic ratio at the end of the storage period, and K+ , Na+ and Cl- concentrations in the red blood cell suspension of both groups (t=0.55, 0.51, 1.18, 0.48, 0.72, 2.86, 2.07, 2.40; P>0.05). ③ In the platelet concentrate of both groups 5 d after storage, all test results of platelet content, red cell volume and pH value at the end of the storage period met the relevant requirements in Quality Requirements for Whole Blood and Blood Components (GB18469-2012). There were no significant differences comparisons of platelet content, red cell volume, level of FHb, platelet adhesion rate, platelet aggregation rate, K+ , Na+ and Cl- concentrations, and pH value in the platelet concentrate of both groups (t=0.17, 0.16, 0.56, 2.43, 0.36, 2.50, 1.85, 1.75, 0.32; P>0.05). Conclusions The quality of the red blood cell suspension and platelet concentrate prepared by whole blood stored at 22 ℃ for 24 h met national standards. Therefore, the separation and preparation of red blood cell suspension and platelet concentrate using whole blood stored at 22 ℃ overnight is feasible. Key words: Blood storage; Blood component removal; Red blood cell; Platelet; Quality control