Compatibility Testing Research Articles

PENERAPAN METODE ISO 9126 DALAM PENGUJIAN KUALITAS SISTEM INFORMASI AKADEMIK SATYA WACANA (SIASAT) UNIVERSITAS KRISTEN SATYA WACANA

The development of information systems has changed human activity, especially education. Universitas Kristen Satya Wacana has made changes by creating a website called SIASAT (Sistem Informasi Akademik Satya Wacana). SIASAT can be accessed by all UKSW communities, features include Student Registration, Course Registration, Study Card, Study Results, lecture schedules, transcripts, student activity, and book borrowing. Previous researchers said SIASAT was still lacking in standardization, so testing was carried out using ISO 9126 with functional and non-functional approaches, containing the characteristics of Functionality, Reliability, Usability, Efficiency, and Portability. The data collection process using Google Forms throughout 132 IT students and interviews with 2019 IT students. The Functional test result scored 80.8%, classified as “good”. Reliability testing can be declared reliable, with a value of 0.874, so exceeds the provision of 0.7. Validity testing results in a table of 0.171 "valid". Non-Functional Results on the Susceptibility Test obtained a “C” score of "medium risk". The Load Testing test got loading time results reaching 3 seconds and 158 MB of memory used "Enough". Stress test results and scalability "Less". The results of the efficiency test obtained a D score of "Less". The Compatibility Test results were very good because SIASAT can be accessed on different browsers. Functional aspects of the approach can be said to be "Good" and Non-Functional Aspects are said to be "Sufficient".

A comprehensive compatibility study of ganciclovir with some common excipients

Objectives: The aim of the present study is to illustrate compatibility testing of ganciclovir (GCV) with some common excipients that would be used to manufacture solid oral dosage forms. Different spectroscopy techniques were utilized to see the interaction of GCV with excipients such as lactose, microcrystalline cellulose (MCC), magnesium stearate, and talc, and dicalcium phosphate. Further, a molecular docking study was also done to know the interaction of GCV with excipients. In vitro study of a physical mixture of GCV with excipients was performed to get the release of drug. Material and Methods: A number of analytical techniques (differential scanning calorimetry [DSC] using DSC-Q20, TA instruments, Fourier-transform infrared spectroscopy [FTIR] spectroscopy using Spectrum RX 1, nuclear magnetic resonance [NMR] using Bruker Advance Neo 500 MHz NMR spectrometer, etc.) have been used to explore the drug-excipient compatibility. Further, a suspected interaction was evaluated by thin-layer chromatography (TLC). In vitro dissolution studies in different sets of experiments were accomplished to determine the influence of hydrophobic and hydrophilic attributes of excipients (MCC, lactose, dicalcium phosphate, and talc) on the dissolution profile of GCV using USP1-type dissolution apparatus. Furthermore, in silico molecular docking studies were also performed to evaluate any probable molecular interactions among drugs and excipients using Auto Dock VINA 1.2.0 software and GROMACS 5.0 software. Results: Comparing FTIR and 1H NMR spectra of GCV and physical mixtures of GCV and excipients, no significant deviation of characteristic peaks in infrared spectroscopy and 1H NMR signals was observed. The DSC of GCV showed two sharp endothermic peaks at 238.82°C and 255°C. The endothermic peak of GCV in DSC thermogram of physical mixtures was observed in nearly the same position except with lactose and dicalcium phosphate. A slightly deviated peak of GCV with a physical mixture of drug and lactose and dicalcium phosphate indicated that there were suspected interactions between the drug with lactose and dicalcium phosphate. These interactions were evaluated by thin-layer chromatography (TLC) and it confirmed that there was no interaction between drugs and excipients. In vitro dissolution studies determined the influence of hydrophobic and hydrophilic attributes of excipients on the dissolution profile of GCV. The physical mixture of GCV with MCC displayed a maximum amount (66.48%) of drug release in 10 min. On the other hand, a physical mixture of GCV with talc showed a minimum amount (12.08%) of drug release in 10 min. Docking study predicted that the number of interactions were more between GCV and lactose (four nos.) in comparison to GCV and MCC (two nos.). This interaction supported the in vitro drug release of a physical mixture of GCV with MCC which was higher than a mixture of GCV with lactose. Conclusion: Compatibility testing of GCV with used excipients by analytical techniques confirmed that GCV should be compatible with used excipients. Drug dissolution of GCV and physical mixture of MCC exhibited the maximum amount of drug release whereas a mixture of GCV with talc released the minimum amount of drug for both short (10 min.) and long (60 min.) periods. Docking studies disclosed that the lactose complex showed less deviation with less root mean square deviation value in comparison to the microcrystalline complex. Thus, the lactose complex has more hydrogen bonds and it was more stable as compared with the MCC complex. GCV indicates that the total energy of the MCC complex is less than that of the lactose complex. This indicates that GCV is more soluble when combined with the microcrystalline complex. Therefore, GCV and used excipients could be used for solid dosage formulations.

Failure probability analysis of hydrogen doped pipelines based on the Bayesian network

Damage from hydrogen, such as hydrogen embrittlement in natural gas pipelines, is a possibility. There are currently lacking ways for calculating the failure of hydrogen doped pipelines quantitatively; instead, research on the safety of them is mostly concentrated on hydrogen compatibility tests and consequence analysis. A method for quantifying hydrogen doped pipelines losses using Bayesian network-based failure probability analysis is presented. The influence of hydrogen on pipeline failure is introduced using the expert scoring method based on the analysis of historical failure data for gas pipelines. The failure probabilities of various components of hydrogen doped pipelines are then quantified by combining fuzzy mathematical theory and hierarchical analysis, which is subsequently utilized as prior probabilities to input into the Bayesian network for quantitative calculation. The results show that the Bayesian network can more accurately quantify the failure probability of hydrogen doped pipelines. They also show that the Bayesian network can provide a practical approach for quantitative risk assessment and safety evaluation, as well as a decision basis for the prevention of hydrogen doping pipeline failure accidents.

Optimizing Overall Equipment Effectiveness Using Kepner-Tregoe Decision Analysis: A Case Study of Semi-lean Pumps in Ammonia Plant 2 of PT Pupuk Kalimantan Timur

The increasing demand for Ammonia production requires innovative approaches to ensure equipment reliability and operational efficiency in its production facility. This study employs a multi-faceted approach to address the challenges associated with the reliability of Semi-lean Pump 107-JB in Ammonia Plant 2 of PT Pupuk Kalimantan Timur, a critical equipment in its production facility. Utilizing the Kepner-Tregoe Decision Analysis Method, the study evaluates various alternative solutions to enhance the pump’s reliability and overall equipment effectiveness (OEE). The research identifies the root causes of the pump’s low reliability, indicated by its declining OEE from 2021-2022. It is done through a series of OEE calculations, 6 Big Losses analysis, and Current Reality Tree analysis. Four major phenomena contributed to the issue: inadequate maintenance procedures and equipment, pump operation beyond its design capacity, insufficient training and competency of manpower, and improper material selection and compatibility testing. The study employs a combination of SCAMPER techniques and the Kepner-Tregoe Decision Analysis framework to develop feasible alternative solutions, which categorizes evaluation criteria into ‘Musts’ and ‘Wants.’ Factors such as cost, feasibility, and potential impact on OEE are assessed. Among the alternatives evaluated, implementing IoT Probe Sensors for real-time monitoring emerged as the most effective solution, addressing the research objective. The thesis culminates in the development and execution of a Maintenance Implementation Plan, which is subsequently assessed through OEE performance metrics. Post-implementation, the Semi-lean Pump 107-JB is estimated to experience an increase in OEE from 63.63% in 2022 to 96.74% in 2024, significantly reducing downtime and contributing an estimated savings of $12.6 million to the company. The research provides actionable insights for improving Semi-lean Pump 107-JB’s reliability. It offers a structured methodology for evaluating alternative solutions for similar applications in industrial settings, particularly rotating equipment in the petrochemical industry.

Significance of red cell alloantibodies other than anti-D during pregnancy and their effect on the newborn: A case series

Contrary to well-established guidelines in developed countries, awareness regarding red cell alloantibodies in antenatal period are lacking in India. Investigating for indirect antiglobulin test (IAT) is mostly limited to the Rh D negative antenatal cases. This case series revisits this vital aspect of maternal and fetal safety. Instances of alloantibody other than anti-D are reported.Study was done in Transfusion Medicine department of a tertiary care hospital in North India during 2019-2020. IAT was performed not during the 1st or 2nd trimesters of pregnancy but as a routine compatibility test during delivery. Patients with positive IAT were further evaluated for the detection of alloantibody by using identification panel red cells. Result: Eight antenatal cases with irregular antibodies other than anti-D during 2019-2020 are described. Antibodies detected per patient were single (three cases of anti-E, one of anti-Fya, one of anti-M) or multiple (two cases of anti-E plus anti-c, one of anti-E plus anti-K). Direct antiglobulin test of four babies born to these mothers was found to be positive, one of whom was still born and rest recovered with medical management. Two other babies had DAT negative and two mothers presented late after still birth. Alloantibody titer indicated in patient with anti-E during mid-pregnancy had titer was undetectable by standard tube technique.Non anti-D alloantibodies can potentially affect fetus, asserting equal attention as anti-D. IAT should not be missed in pregnancy as it is common to investigations for compatibility as well as for fetal wellbeing assessment.

Development and evaluation of the in vitro schistosomicidal activity of solid dispersions based on 2-(-5-bromo-1-H-indole-3-yl-methylene)-N-(naphthalene-1-ylhydrazine-carbothiamide

Among all the neglected diseases, schistosomiasis is considered the second most important parasitic infection after malaria. Praziquantel is the most widely used drug for this disease, but its exclusive use may result in the development of drug-resistant schistosomiasis. To increase the control of the disease, new drugs have been developed as alternative treatments, among them 2-(-5-bromo-1-h-indole-3-yl-methylene)-N-(naphthalene-1-ylhydrazine-carbothiamide (LQIT/LT-50), which showed promising schistosomicidal activity in nonclinical studies. However, LQIT/LT-50 presents low solubility in water, resulting in reduced bioavailability. To overcome this solubility problem, the present study aimed to develop LQIT/LT-50 solid dispersions for the treatment of schistosomiasis. Solid dispersions were prepared through the solvent method using Soluplus©, polyethylene glycol (PEG) or polyvinylpyrrolidone (PVP K-30) as hydrophilic carriers. The formulations with the best results in the compatibility tests, aqueous solubility and preliminary stability studies have undergone solubility tests and physicochemical characterizations by Fourier-transform infrared spectroscopy (FTIR), x-ray diffractometry (XRD), exploratory differential calorimetry (DSC), thermogravimetry (TG) and Raman spectroscopy. Finally, the schistosomicidal activity was evaluated in vitro. The phycochemical analyzes showed that when using PVP K-30, there was an interaction between the PVP K-30 and LQIT/LT-50, proving the successful development of the solid dispersion. Furthermore, an increase in the solubility of the new system was observed (LQIT/LT-50:PVP K-30) in addition to the improvement in the in vitro shistosomidal activity at 1:4 (w/w) molar ratio (i.e., 20% drug loading) when compared to LQIT/LT-50 alone. The development of the LQIT/LT-50:PVP K-30 1:4 solid dispersion is encouraging for the future development of new pharmaceutical solid formulations, aiming the schistosomicidal treatment.

Blood donation practices, processing and utilisation of blood components in government tertiary hospitals in Nigeria: a multicentre cooperative study.

Timely access to safe blood and blood components is still a challenge in Nigeria. This study aimed to determine blood donation practices, processing and utilization of blood components across government tertiary hospitals (THs) in Nigeria. This was a descriptive cross-sectional study done in Nigeria in June-July 2020. Data were analysed with SPSS version 21.0. Data were collected from 50 THs. The majority (68%) of the THs lack facilities for blood component preparation and only 18% and 32% provide cryoprecipitate and platelet concentrate, respectively. Whole blood was most commonly requested (57.04%). All facilities tested blood for HIV, HBV and HCV, but the majority (23 [46%]) employed rapid screening tests alone and nucleic acid testing was not available in any hospitals. The manual method was the most common method of compatibility testing in 90% (45/50) and none of the THs routinely perform extended red cell typing. The average time to process routine, emergency and uncross-matched requests were a mean of 109.58±79.76min (range 45.00-360.00), 41.62±25.23 (10.00-240.00) and 11.09±4.92 (2.00-20.00), respectively. Facilities for blood component preparation were not widely available. Concerned government authorities should provide facilities for blood component preparation.

Microrheological characterisation of Cyanoflan in human blood plasma

Naturally occurring polysaccharidic biopolymers released by marine cyanobacteria are of great interest for numerous biomedical applications, such as wound healing and drug delivery. Such polymers generally exhibit high molecular weight and an entangled structure that impact the rheology of biological fluids. However, biocompatibility tests focus not so much on rheological properties as on immune response. In the present study, the rheological behaviour of native blood plasma as a function of the concentration of a cyanobacterium biopolymer is investigated via multiple particle tracking microrheology, which measures the Brownian motion of probes embedded in a sample, and cryogenic scanning electron microscope microstructural characterisation. We use Cyanoflan as the biopolymer of choice, and profit from our knowledge of its chemical structure and its exciting potential for biotechnological applications. A sol–gel transition is identified using time-concentration superposition and the power-law behaviour of the incipient network's viscoelastic response is observed in a variety of microrheological data. Our results point to rheology-based principles for blood compatibility tests by facilitating the assignment of quantitative values to specific properties, as opposed to more heuristic approaches.

Dimethyl fumarate modulates the dystrophic disease program following short-term treatment.

New medicines are urgently required to treat the fatal neuromuscular disease, Duchenne muscular dystrophy (DMD). Dimethyl fumarate (DMF) is a potent immunomodulatory small molecule nuclear erythroid 2-related factor 2 (Nrf2) activator with current clinical utility in the treatment of multiple sclerosis and psoriasis that could be effective for DMD and rapidly translatable. Here, we tested two weeks of daily 100mg/kg DMF versus 5mg/kg standard care prednisone (PRED) treatment in juvenile mdx mice with early symptomatic DMD. Both drugs modulated seed genes driving the DMD disease program and improved force production in fast-twitch muscle. However, only DMF showed pro-mitochondrial effects, protected contracting muscles from fatigue, improved histopathology and augmented clinically compatible muscle function tests. DMF may be a more selective modulator of the DMD disease program than PRED warranting follow-up longitudinal studies to evaluate disease modifying impact.

Curcumin-encapsulated fish gelatin-based microparticles from microfluidic electrospray for postoperative gastric cancer treatment

Gastric cancer is the fifth most frequently diagnosed malignant neoplasm and the third leading cause of cancer-related mortality. Nevertheless, the therapeutic efficacy of conventional surgical and chemotherapeutic interventions in clinical practice is often unsatisfactory. Curcumin (Cur) has shown promise as a therapeutic agent in prior studies. However, its progress in this context has been impeded by challenges including low solubility, instability in aqueous environments, and rapid metabolism. In this study, we develop methacrylate fish gelatin (FGMA) hydrogel microparticles (FGMPs@Cur) encapsulating Cur via microfluidic electrospray technology for postoperative comprehensive treatment of gastric cancer. Comprehensive characterizations and analyses were conducted to assess the cytotoxicity against gastric cancer cells and potential tissue reparative effects of FGMPs@Cur. In vitro experiments revealed that FGMPs@Cur exhibited a remarkable cytotoxic effect on nearly 80 % of gastric cancer cells while maintaining at least 95 % viability of normal cells in cell compatibility tests. In vivo results demonstrated that FGMPs@Cur significantly reduced tumor volume to 47 % of the control group, and notable tissue regeneration was observed at the surgical site. These properties indicated that such a hydrogel microparticle system is a promising candidate for postoperative gastric cancer treatment in practical application.

STUDY OF PACKER'S SWELLING RATE IN DIFFERENT LIQUIDS

Cementing is currently the only method used in oil fields to establish zonal isolation. Another method for zonal isolation involves the use of swellable packers. Swellable packers are designed using elastomeric components that have a unique swelling property when exposed to water, water-based fluids or oil. The swelling of the elastomer creates a tight seal. Swellable packers offer several advantages in well construction and maintenance. They can be used for a variety of purposes including preventing gas migration, shutting off water flow, and facilitating fracturing operations. Swellable packer technology involves the selection of elastomers that can expand in the presence of water, oil, or certain brines. It is assumed that the degree of swelling and rate of expansion depends on factors such as elastomer composition, conditions in the wellbore, and the salinity of the surrounding fluids. The use of swellable packers requires careful consideration of well conditions and the fluids present. Matching the packer's swelling properties to the intended well’s fluids is essential to achieve effective zonal isolation. Proper elastomer compatibility testing and evaluation is recommended prior to field deployment to ensure successful and reliable packer operation. in this regard, the purpose of this study is to experimentally confirm the possibility of expanding packers in fluid with different salt concentrations and oil. The study highlights the importance of conducting a comprehensive quality assessment of elastomers prior to their use in field operations. Keywords: cementing, zonal isolation, swellable packers, swelling property, salinity, elastomers, crude oil, swelling rate.

Lipid-Based Nano-Formulation Development to Enhance Oral Bioavailability of Weakly Aqueous-Soluble Drug for Obesity and Hypertension

The most practical method of drug delivery is oral administration because it has a high rate of patient compliance. However, there are significant obstacles to effective oral medication delivery, including low drug enzymatic/metabolic stability and poor water solubility. Especially in the development of drug formulations for the treatment of obesity and hypertension. This research article aims to formulate solid lipid nanoparticles (SLN) of Fucoxanthin and Ramipril by the emulsification and ultrasonication methods. The nanoparticles size, polydispersity index, and the zeta potential, among other parameters, were computed. In addition, FT-IR analysis of compatibility tests between the SLNs and the loaded drug and in vitro drug release experiments were carried out. Lipid-based nano preparations have drawn plenty of interest as efficient vehicles to increase the oral bioavailability of these kinds of medications. We observed that lipid nanoparticles, have enhanced the oral bioavailability of poorly water-soluble drugs used for obesity and hypertension. Provided the above information, formulated SLNs should be further investigated using cutting-edge scientific methodologies to improve its bioavailability.

Characteristics of polypropylene composites filled with palm oil-modified cellulose powder

Hydrophilic cellulose powder was modified with palm oil for hydrophobicity to strengthen the interfacial bonding between cellulose powder and hydrophobic polypropylene (PP). The palm oil-modified cellulose powders with various palm oil contents were analyzed by compatibility test, FTIR spectroscopy and TGA. The IR absorption peak for the ester bridges between cellulose powder and palm oil increased with the palm oil content attached to the cellulose powder. The PP/cellulose (80/20 by weight) composites with the pristine or one of the modified cellulose powders were prepared by melt blending followed by compression molding. The mechanical properties of the composites were measured by an impact and tensile test. The impact strength, elongation at break and water-resistance increased but tensile modulus decreased with the palm oil content. The composite with 4.87 wt.% of the palm oil showed optimum tensile properties. The SEM images showed that the interfacial bonding between the cellulose powders and the PP matrix was strengthened by the modification of the cellulose powder with the palm oil.

Pengoptimalan Komunikasi Bahasa Isyarat Abjad dengan Augmented Reality

One of the activities that humans always do is communication. This communication is carried out by everyone, but there are special ways of communication that are carried out by people with special needs, such as the deaf. The communication uses sign language, where learning the language is quite difficult, especially for students who are deaf. There is a solution that can solve this problem, namely the use of technology. One of the technologies that can be used is Augmented Reality. Augmented Reality is able to combine the physical world and digital elements that can be used to facilitate human activities. Augmented Reality is designed and packaged in the form of a mobile-based application to make it easier to learn sign language. The use of this designed application is specifically aimed at students with special needs. In addition, the use of the application can also be felt by a group of people who are learning sign language. Application testing involves various aspects, such as functional testing, device compatibility testing, performance testing, AR accuracy and compatibility testing, interaction testing, usability testing, and testing in real environments. App testing engages users, either in user testing or through the feedback they provide. The users involved are divided into 2, namely users as experts in AR testing and users as customers or in this case teachers. The test results prove the value with a high percentage. Tests by experts achieve an average value of 93.13% and tests by customers achieve an average value of 93.60%. This average value can be classified as a reference that the application functions properly, according to needs, is safe, responsive, and provides a satisfying experience.

Electromagnetic Compatibility Testing Method and Application of Generators Based on Flexible Loading System

As an important part of the extended-range new-energy vehicle, the electromagnetic compatibility of the generator system will affect the overall performance of the vehicle. Therefore, it is necessary to conduct electromagnetic compatibility testing on the generator system, which includes two parts: electromagnetic emission and electromagnetic immunity. By implementing EMC testing of generators based on flexible loading equipment, we simulate the working state of the generator system during the real vehicle operation in the 3m anechoic chamber and evaluate the electromagnetic compatibility performance of the generator system. Flexible loading equipment can solve the adverse effects of large engine system volume, high noise, and exhaust emissions on the anechoic chamber environment in extended-range new-energy vehicles, and obtain real-time testing information of the generator system, achieving the standard requirements of quantifiable and evaluable test results.

Expression of defense responsive genes in tripartite interaction of cucumber mosaic virus and plant growth promoting rhizobacteria in ridge gourd (Luffa acutangula (L.) Roxb)

The mosaic caused by cucumber mosaic virus (CMV) on ridge gourd (Luffa acutangula (L.) Roxb) is an important disease. An attempt was made to use a consortium of plant growth promoting rhizobacteria to manage the disease with an aim to reduce the usage of pesticide to control its vector. Eleven isolates of Bacillus spp. were selected based on their plant gowth promotion ability on ridge gourd cv. COH1. Among these, Bacillus pumilus (BSC 4), B. subtilis (Bbv 57) and B. subtilis (BST 8) had shown their potentiality in reducing the severity of CMV disease under artificial conditions. The compatibility test showed that these isolates were compatible to each other. A Bacillus consortium was prepared and evaluated for its efficacy against CMV on ridge gourd and the results showed a reduction of 49.98 % in disease severity over control. The quantitative gene expression analysis revealed that the defense genes viz., peroxidase, polyphenol oxidase, catalase and super oxide dismutase were upregulated in consortium treated ridge gourd plants. The upregulation of these genes indicated the activation of defense pathways which witnessed the induction of systemic resistance in Bacillus consortium treated plants. All gene expressions were reached its peak nearly two to three fold in the consortium treated plants except catalase, its peak expression was found in salicylic acid treated plants which served as a positive control. Correspondingly, the quantitative enzymatic analysis of these defense enzymes at different time intervals was also reflected in similar manner. The elevated level of defense enzymes in plant growth promoting rhizobacteria consortium treated plants was the evident for the onset of systemic resistance.

Design and implementation of student work management system in the context of deep learning

Abstract This paper aims to analyze and design the system’s functions by student work management requirements, focusing on the business and user modules. The security testing algorithm based on the deep learning model verifies the system’s operation, analyzes the adversarial sample attack in image and text scenarios, and uses a black-box-white-box attack algorithm and defense algorithm for system security testing. Perform environment testing, compatibility testing, data response performance testing, and instance model evaluation for implementing the student work management system. Less than 500ms is the response time, over 400 requests are processed per second, and the timeout response rate is below 5%. The security evaluation coefficient was 66.982 for Example Model I and 74.628 for Example Model II, showing the system has good loadability and security.

Prevalence of A2 and A2B Subgroups among Blood Groups A and AB in healthy donors.

To determine the frequency of A2 and A2B subgroups among blood groups A and AB in healthy donors. It was a Cross-Sectional study, conducted at the Department of Hematology & Transfusion Medicine, UCHS, The Children's Hospital Lahore and Sundas foundation Lahore from June 2022 to December 2022 including 13,120 healthy blood donors of both genders, after taking informed consent. Venous blood samples of donors were collected in EDTA vials (3ml) and serum gel vial for routine blood grouping which was done by standard tube method. Further testing of donors positive for an antigen (blood Group-A and AB) was performed using anti-A1 lectin by standard tube method as per manufacturer's instruction. The data was analyzed using SPSS version 23. Among 13120 blood donors, 12857 (97.9%) were male and 263 (2.0%) were female with mean age of 36.7 years ± 15.04 years. Majority of them (91.7%) were of Punjabi ethnicity. Donors having blood group phenotype A and AB were 3890 (29.6%). Among blood Group-A donors, A1 was found in 97.8% and A2 in 2.2% donors. While among Blood Group-AB, 96.7% donors belonged to A1B blood group and 3.2% belonged to A2B blood group. Blood group A2 and A2B do exist in blood donors of Punjabi ethnicity. The knowledge of presence of these blood groups' phenotypes in our population can provide a better base for transfusion staff to do troubleshooting in compatibility testing and to avoid any rare but hazardous transfusion outcome.

Effect of the Thermophilic Bacterial Biculture Consortium from Mudiak Sapan Hot Springs on Biofuel Production

Biofuels are fuels derived from biomass and consist of biogas, biodiesel and bioethanol. Bioethanol is a biofuel whose main ingredients are from plants and generally use microorganisms in the fermentation process. One way is to use thermophilic bacteria with the advantage of low contamination levels and better product quality. Bacteria that exist in nature are not only in a single form but also exist in the form of a consortium in which there are beneficial or detrimental bacterial interactions. Favorable bacterial interactions indicate that the bacteria are compatible. Using a consortium with compatible bacteria gives better results than using a single bacteria. This study aims to determine the compatibility and effect of a consortium of thermophilic bacteria from Mudiak Sapan hot springs on biofuel yields. This research is a descriptive research. To test the cooperation between isolates, a compatibility test was carried out using the disk diffusion method. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%. Then the biculture consortium isolates of thermophilic bacteria were fermented in liquid TMM medium (Thermophilic Minimum Media) and the bioethanol content was measured using a distillation apparatus. The results of this study showed that the six pairs of compatible consortium and MS 9-12 consortium produced the highest bioethanol, namely 1.0003%.

Y-site injection physical compatibility of remdesivir with select intravenous drugs used in palliative care and for treating coronavirus disease 2019.

No compatibility tests are available for remdesivir other than 0.9% sodium chloride. In this study, we aimed to evaluate the physical compatibility of remdesivir with drugs used in palliative care and COVID-19 treatment. Remdesivir was tested for compatibility with 10 different drugs (fentanyl, morphine, hydromorphone, oxycodone, heparin, furosemide, octreotide, acetated Ringer's injection, 2-in-1 peripheral parenteral nutrition, and 2-in-1 total parenteral nutrition). Remdesivir was formulated to a final concentration of 1 mg/mL, and the other drugs were prepared at clinical concentrations. Three test solutions were used for compatibility testing, with remdesivir and the target drugs compounded in a 1:1 ratio. Appearance measurements, including Tyndall effect, turbidity, and pH, were performed immediately after mixing and at 1 h and 4 h after mixing. Changes in appearance, including the Tyndall effect, turbidity (turbidity change of ≥ 0.5 nephelometric turbidity unit [NTU] based on control solution for each test drug), and pH (a change of ≥ 10% based on the pH immediately after mixing) were used to determine physical compatibility. All the drugs tested were compatible with remdesivir. The combination of remdesivir and furosemide produced the highest turbidity (0.23 ± 0.03 NTU) 1 h after mixing. The lowest and highest pH values were observed at 4 h after mixing for the combinations of remdesivir and morphine (3.23 ± 0.02) and remdesivir and furosemide (8.81 ± 0.06). The drugs tested in this study show Y-site physical compatibility with remdesivir.