Comparative Performance Evaluation Research Articles

Comparative Performance Evaluation of High-Voltage Bidirectional, Conventional and Superjunction Planar DMOSFETs in 4H-SiC

We quantitively compare the static and dynamic performance for high-voltage SiC bidirectional (BD) conventional and superjunction (SJ) DMOSFETs by using 3D TCAD simulations. We extract the specific on-resistance (RON,sp) and the total specific switching charge (QT,sp), which is a sum of the specific gate charge (QG,sp) and drain charge (QDS,sp) to quantify both the static and switching characteristics respectively. We also develop a new Figure-of-Merit (FoM), which is the product of RON,sp . QT,sp, to evaluate the overall performance. We show that the high-voltage 4H-SiC BD SJ DMOSFET has the best FoM with substantial (>58%) improvement, compared to the BD conventional DMOSFETs, which increases with increasing breakdown voltage.

Comparative Evaluation of Performance of Traditional Method and Commercially Available Pedal Operated Paddy Thresher with the Developed Machine

Traditional method of threshing of the Paddy crop is categorized as very laborious, low output, high damage per cent, costly etc. The present study was conducted to design and develop a power operated paddy drum thresher cum winnower to increase the mechanization in paddy crop threshing process, reduce drudgery, and increase production and to make it cost effective etc. Different components of the power operated paddy drum thresher cum winnower were designed and developed at Department of Farm Machinery and Power Engineering, SVCAET and RS, FAE, IGKV RAIPUR. It consisted of threshing drum, blower, reciprocating sieve, lower sieve and upper sieve etc.The data were analyzed and optimized by Response Surface Method (RSM) using Design Expert 13.0 software. The optimum values of the independent variables of threshingunit viz., feed rate 275kg/h, peripheral speed 11 m/s and moisture 14.5% (db) the corresponding values of dependent parameters of threshing unit viz., threshing efficiency 98.7%, threshing capacity 210 kg/h, cleaning efficiency 90.1%and total grain loss percent1.96 were recorded. The total cost of operation was observed to be Rs. 1.04 per kg with breakeven point and payback period of 124.13hours per year and 2 years respectively.

Comparative Evaluation of Microwave Heating Performance across Structurally Diverse Tungsten Oxides

Comparative Evaluation of Microwave Heating Performance across Structurally Diverse Tungsten Oxides

Mitigation of fouling with swirling flow induced in 3-D double-sided straight channel for printed circuit heat exchanger

Fouling should be considered in the design of a printed circuit heat exchanger (PCHE) for industrial applications. The swirling flow, which is known to mitigate fouling in heat exchangers, can incorporate this aspect; however, realizing these flow characteristics in compact heat exchangers such as PCHE is difficult. Thus, this study proposed a three-dimensional (3-D) designed PCHE channel. To induce swirling flow, it was designed to face two semicircular straight channels with angles such that the interaction between the mainstreams in different flow directions induced a swirling flow. Compared to conventional two-dimensional (2-D) PCHE channels (straight, zigzag, and airfoil channels), the swirling flow induced in the proposed 3-D PCHE channel uniformly increased the wall shear stress while minimizing flow separation or recirculation. This minimized the low wall shear stress that was more likely to be fouled. A comparative evaluation of the thermal-hydraulic performance of the investigated channels was conducted by comparing the volume goodness factors under the equal hydraulic diameter. The proposed 3-D channels exhibited the potential to mitigate fouling, as well as a thermal-hydraulic performance comparable to that of conventional 2-D PCHE channels. Therefore, the proposed 3-D channel can be suggested as an advanced PCHE design option for high thermal-hydraulic performance and mitigation of fouling. Furthermore, because the swirling flow is known to mitigate fouling in heat exchangers as well as the heat transfer deterioration of sCO2, a double-sided straight channel with swirling flow is expected to be competitive.

Investigations of BLDC motor speed characteristics via THD under conventional and advanced hybrid controllers

This project investigates brushless direct current (BLDC) motor speed control through total harmonic distortion (THD) analysis, employing proportional integral (PI), fuzzy logic (FLC), adaptive neuro-fuzzy inference system (ANFIS), and an innovative hybrid ANFIS-PD/PI controller. Considering the vital role of BLDC motors in precision-dependent industries like robotics, electric vehicles, and industrial automation, our primary focus is on understanding BLDC motor operation and recognizing THD's significance as a performance metric. Controllers are meticulously implemented in real-time, fine-tuned, and optimized to achieve desired speed characteristics, incorporating considerations like response time, accuracy, and energy efficiency. The project's core involves THD analysis, quantifying harmonic content in the BLDC motor's speed waveform. This facilitates a comprehensive comparative evaluation of controller performance, assessing their capability to maintain speed stability and influence power quality. The discussion covers the merits and limitations of each controller, with a special emphasis on the hybrid ANFIS-PD/PI controller, seamlessly blending ANFIS adaptability with PD/PI control stability. Results illustrate the hybrid controller's excellence in optimizing BLDC motor speed control, demonstrating superior performance in speed accuracy, disturbance rejection, and THD reduction. These findings drive advancements in motor control technology, providing practical guidance for selecting controllers tailored to specific application requirements. Simulation results can be analyzed using MATLAB/Simulink 2018a Software.

Comparative Performance Evaluation of Active Greenhouse Solar Dryer with and without Reflective Floor Material

Comparative Performance Evaluation of Active Greenhouse Solar Dryer with and without Reflective Floor Material

Comparative performance evaluation of random access and real-time PCR techniques in the diagnosis of BK virus infections in transplant patients

PurposeThe study aims to compare random-access NeuMoDx values with artus qPCR values to validate the accuracy of NeuMoDx as an alternative to qPCR and provide an equation to convert copies/ml to IU/ml measurements. MethodsA total of 95 samples, including 61 transplant patient samples (n = 23 urine, n = 38 plasma) as the study group, 28 BKPyV-free samples as the control group, and six quality control samples, were included. One-Way ANOVA, Pearson correlation, Bland-Altman, Passing-Bablok, Deming regression analyses were used for statistical evaluation. ResultsOf 95 samples, 46 (48 %) were positive with NeuMoDx, while 40 (42 %) were positive with artus qPCR. Both techniques were statistically similar (p > 0.05). Deming correlation analysis (r = 0.9590), Passing Bablok and Bland Altman analyses demonstrated a strong correlation between NeuMoDx and artus values. The equation that provides the conversion between NeuMoDx and artus qPCR values was NeuMoDx= (1.12965 x artus qPCR) – 0.55016. BKPyV infections remain a concern for transplant patients globally, and effective new diagnostic methods are required. ConclusionsConsistency between the results of NeuMoDx and qPCR confirms that NeuMoDx may be a valuable alternative for detecting BKPyV to prevent viral propagation. Our findings may allow converting copy/ml results to IU/ml for diagnosing and monitoring BKPyV infections in transplant patients.

Comparative performance evaluation of dual-axis solar trackers: Enhancing solar harvesting efficiency

Nowadays, renewable energy is a much discussable topic because of its important specifications such as pollution and un end sources. This paper emphasizes the importance and capabilities of the dual axis solar tracking system (DASTS) of solar cell technologies. The study carried out a practical process of a dual-axis system design, which practically demonstrates the influence of dual-axis solar trackers, including their operational principles, advantages, and associated challenges. Furthermore, it has been shown that dual-axis solar trackers can significantly increase solar energy yield by capturing maximum sunlight, making them an important advancement in improving the efficiency and effectiveness of solar energy generation. The paper also presents a performance evaluation of a solar tracker system that improved energy output by 45 % compared to the fixed solar panel (FSP) while also reducing the numerical calculation (NC) efficiency of DASTS by 3.16 %. Based on these findings, solar tracker systems can provide sustainable and environmentally friendly energy solutions that are both feasible and substantial.

Specificity of serological screening tests and reference laboratory tests to diagnose gambiense human African trypanosomiasis: a prospective clinical performance study

BackgroundSerological screening tests play a crucial role to diagnose gambiense human African trypanosomiasis (gHAT). Presently, they preselect individuals for microscopic confirmation, but in future “screen and treat” strategies they will identify individuals for treatment. Variability in reported specificities, the development of new rapid diagnostic tests (RDT) and the hypothesis that malaria infection may decrease RDT specificity led us to evaluate the specificity of 5 gHAT screening tests.MethodsDuring active screening, venous blood samples from 1095 individuals from Côte d’Ivoire and Guinea were tested consecutively with commercial (CATT, HAT Sero-K-SeT, Abbott Bioline HAT 2.0) and prototype (DCN HAT RDT, HAT Sero-K-SeT 2.0) gHAT screening tests and with a malaria RDT. Individuals with ≥ 1 positive gHAT screening test underwent microscopy and further immunological (trypanolysis with T.b. gambiense LiTat 1.3, 1.5 and 1.6; indirect ELISA/T.b. gambiense; T.b. gambiense inhibition ELISA with T.b. gambiense LiTat 1.3 and 1.5 VSG) and molecular reference laboratory tests (PCR TBRN3, 18S and TgsGP; SHERLOCK 18S Tids, 7SL Zoon, and TgsGP; Trypanozoon S2-RT-qPCR 18S2, 177T, GPI-PLC and TgsGP in multiplex; RT-qPCR DT8, DT9 and TgsGP in multiplex). Microscopic trypanosome detection confirmed gHAT, while other individuals were considered gHAT free. Differences in fractions between groups were assessed by Chi square and differences in specificity between 2 tests on the same individuals by McNemar.ResultsOne gHAT case was diagnosed. Overall test specificities (n = 1094) were: CATT 98.9% (95% CI: 98.1–99.4%); HAT Sero-K-SeT 86.7% (95% CI: 84.5–88.5%); Bioline HAT 2.0 82.1% (95% CI: 79.7–84.2%); DCN HAT RDT 78.2% (95% CI: 75.7–80.6%); and HAT Sero-K-SeT 2.0 78.4% (95% CI: 75.9–80.8%). In malaria positives, gHAT screening tests appeared less specific, but the difference was significant only in Guinea for Abbott Bioline HAT 2.0 (P = 0.03) and HAT Sero-K-Set 2.0 (P = 0.0006). The specificities of immunological and molecular laboratory tests in gHAT seropositives were 98.7–100% (n = 399) and 93.0–100% (n = 302), respectively. Among 44 reference laboratory test positives, only the confirmed gHAT patient and one screening test seropositive combined immunological and molecular reference laboratory test positivity.ConclusionsAlthough a minor effect of malaria cannot be excluded, gHAT RDT specificities are far below the 95% minimal specificity stipulated by the WHO target product profile for a simple diagnostic tool to identify individuals eligible for treatment. Unless specificity is improved, an RDT-based “screen and treat” strategy would result in massive overtreatment. In view of their inconsistent results, additional comparative evaluations of the diagnostic performance of reference laboratory tests are indicated for better identifying, among screening test positives, those at increased suspicion for gHAT.Trial registrationThe trial was retrospectively registered under NCT05466630 in clinicaltrials.gov on July 15 2022.Graphical

Comparative Performance Evaluation of Ultrasonic Welding and Conventional Sewing Methods in Knitted Fabrics

Abstract When transforming two-dimensional fabric pieces into three-dimensional clothing with different sewing methods, choosing the proper technique is one of the primary factors affecting the quality of the product. Within the scope of the study, five different types of knitted fabric alternatives, which are frequently used in products for daily use, are discussed. The conventional joining methods (lock stitch and overedge stitching) and ultrasonic welding were evaluated in terms of joint performance. All the evaluations made in terms of seam strength and seam efficiency showed that the overedge stitch is more performant in sewing knitted fabrics. One of the critical advantages of ultrasonic welding, the absence of the need for needle and thread and the absence of these costs, necessitated the evaluation of one more feature of the joining methods in the study. In this context, the energy consumption values of all the methods were evaluated. As a result, the energy consumption value of ultrasonic welding is approximately 1.3 times higher than overedge stitching and approximately 10.7 times higher than lockstitching.

Preparation and Performance Study of SiC-Reinforced Fe-Based Wear-Resistant Composite Grinding Media.

During industrial and laboratory processes involving material grinding, the grinding media endure prolonged high-collision and friction environments, resulting in substantial wear. Consequently, this study adopts the hot-pressing sintering technique in powder metallurgy to prepare SiC-reinforced Fe-based wear-resistant composite grinding media, aiming to increase wear performance. For this purpose, Fe with 10 wt% SiC powders were milled for the fabrication of the composite. Then, sintering was performed by hot press at 1100 °C in a furnace. Scanning electron microscopy (SEM) and X-ray diffraction were employed to investigate the microstructures and phase of SiC-reinforced Fe-based matrix composite. Subsequently, comparative performance evaluations of the newly developed grinding media and traditional chromium-based media were conducted in terms of wear rate and grinding efficiency. The wear resistance tests revealed that the SiC-reinforced composite media displayed significantly superior wear resistance across various abrasives compared to the chromium-containing alternatives. Specifically, the composite media achieved a wear rate reduction of 2.9 times against standard sand over 1 h, and 2.3 and 2.4 times against sandstone and iron slag, respectively. Moreover, extended grinding for 3 hours further enhanced these reductions to 3.1, 2.4, and 2.7 times, respectively. Additionally, efficiency assessments indicated that at a 1:1 material ratio, the composite media outperformed the chromium-containing media in grinding efficiency by 7.5%, 12.5%, and 10.3% for standard sand, sandstone, and iron slag, respectively. Further increasing the material ratio to 3:1 resulted in efficiency improvements of 7.4%, 17.5%, and 11.3%, correspondingly.

Comparative evaluation of intubator performance and time taken for intubation in COVID patients using two different barrier devices requiring emergency endotracheal intubation: A randomized cross over study

BackgroundThe COVID-19 pandemic has necessitated use of barrier devices, such as goggles and face shields, during endotracheal intubation procedures to protect healthcare professionals. While there is existing research on the efficacy of these devices in simulated environments, there is a lack of published studies comparing their utility in real COVID-19 cases. This randomized crossover study aimed to compare the performance of face shields and goggles during endotracheal intubation in actual COVID-19 cases. MethodsThe study was registered in Clinical Trials Registry of India with registration no. CTRI/2022/07/044114 and was conducted at a tertiary care hospital emergency Operartion theatres (OTs) from May 2022 till March 2023. Experienced airway management professionals participated in the study, with a sample size of 128 participants: using either face shields (Group F) or goggles (Group G). The participants performed intubation attempts using both face shields and goggles in a crossover manner. Subgroups were formed based on whether participants wore medically prescribed spectacles or not. The numerical variables were analyzed by Mann-Whitney U test/Unpaired t-test. The categorical variables were analyzed by Chi-square test/Fischer exact-test. ResultsThe overall intubation time was significantly shorter in face shield group (12.75 ± 1.84 s) compared to the goggles group (14.30 ± 1.96 s) (P < 0.005). Additionally, the time taken for endotracheal tube insertion into the glottis was significantly shorter in the face shield group (3.52 ± 1.22 s) compared to the goggles group (4.26 ± 1.37 s) (P < 0.005). Participants rated the vision and clarity higher in the face shield group (P < 0.001). However, they found face shields to be more claustrophobic compared to goggles (P < 0.001). ConclusionThe study concludes that face shields may offer benefits in terms of faster intubation times, improved vision and clarity, better ergonomics and also more suited for healthcare workers with prescribed spectacles, but potential claustrophobic effects to be kept in mind while selecting protective equipment for healthcare professionals performing intubations.

Comparative Performance Evaluation of Double-Stranded RNA High-Throughput Sequencing for the Detection of Viral Infection in Temperate Fruit Crops.

There is limited information on the compared performances of biological, serological. and molecular assays with high-throughput sequencing (HTS) for viral indexing in temperate fruit crops. Here, using a range of samples of predetermined virological status, we compared two performance criteria (inclusivity and analytical sensitivity) of enzyme-linked immunosorbent assay (ELISA), molecular hybridization, reverse transcription (RT)-PCR, and double-stranded RNA (dsRNA) HTS for the detection of a total of 14 viruses (10 genera) and four viroids (three genera). When undiluted samples from individual plants were used, ELISA had the lowest performance, with an overall detection rate of 68.7%, followed by RT-PCR (82.5%) and HTS (90.7%; 100% if considering only viruses). The lower performance of RT-PCR reflected the inability to amplify some isolates as a consequence of point mutations affecting primer-binding sites. In addition, HTS identified viruses that had not been identified by other assays in nearly two-thirds of the samples. Analysis of serial dilutions of fruit tree samples allowed comparison of analytical sensitivities for various viruses. ELISA showed the lowest analytical sensitivity, but RT-PCR showed higher analytical sensitivity than HTS for most of the samples. Overall, these results confirm the superiority of HTS over biological indexing in terms of speed and inclusivity and show that while the absolute analytical sensitivity of RT-PCR tends to be higher than that of HTS, PCR inclusivity is affected by viral genetic diversity. Taken together, these results make a strong case for the implementation of HTS-based approaches in fruit tree viral testing protocols supporting quarantine and certification programs.

FPGA-BASED IMPLEMENTATION OF A GAUSSIAN SMOOTHING FILTER WITH POWERS-OF-TWO COEFFICIENTS

The purpose of the study is to develop methods for synthesizing a Gaussian filter that ensures simplified hardware and software implementation, particularly filters with powers-of-two coefficients. Such filters can provide effective denoising of images, including landscape maps, both natural and synthetically generated. The study also involves analyzing of methods for FPGA implementation, comparing their hardware complexity, performance, and noise reduction with traditional Gaussian filters. Results. An algorithm for rounding filter coefficients to powers of two, providing optimal approximation of the constructed filter to the original, is presented, along with examples of developed filters. Topics covered include FPGA implementation, based on the Xilinx Artix-7 FPGA. Filter structures, testing methods, simulation results, and verification of the scheme are discussed. Examples of the technological placement of the implemented scheme on the FPGA chip are provided. Comparative evaluations of FPGA resources and performance for proposed and traditional Gaussian filters are carried out. Digital modeling of the filters and noise reduction estimates for noisy images of the terrain surface are presented. The developed algorithm provides approximation of Gaussian filter coefficients as powers of two for a given window size and maximum number of bits with a relative error of no more than 0.18. Implementing the proposed filters on FPGA results in a hardware costs reduction with comparable performance. Computer simulation show that Gaussian filters both traditional and proposed effectively suppress additive white noise in images. Proposed filters improve the signal-to-noise ratio within 5-10 dB and practically match the filtering quality of traditional Gaussian filters.

Performance evaluation of ruthenium complexes and organic sensitizers in ZnO-based dye-sensitized solar cells

Ruthenium (Ru) dyes are a well-known player in the field of dye-sensitized solar cells (DSSCs) due to their high efficiency and excellent stability. Their properties and complexes have been studied for almost three decades. Although these sensitizers show better performances, their high cost makes these third-generation solar devices less economical. Organic dyes have recently been explored as an alternative to Ru-based dyes due to their easy and low-cost synthesis. A comparative performance evaluation of Ru complexes and dicyanoisophorone and rhodanine organic dyes in ZnO-based DSSCs is here reported. All the Ru complexes showed better performance in comparison to organic dyes except R-4. Among the Ru sensitizers, R-3 exhibited the highest efficiency of 1.21% followed by R-2, which is attributed to the presence of several anchoring groups such as carboxyl, nitro and amine. However, the presence of more nitrogen-based groups has drastically reduced the performance as observed for R-4, which is the least performing dye among the Ru-based ones. On the contrary, organic sensitizers S-06 and P-4 revealed to be less efficient with respect to R-3 owing to the presence of only one anchoring group and weak photoanode/dye interaction.

Comparative performance evaluation of voltage gate-spin orbit torque MTJ-based digital logic circuits with 45 nm CMOS technology

The development of advanced logic systems relies on low-power, compact devices with incredibly fast computational speeds. Voltage gate spin–orbit Torque Magnetic Tunnel Junction has emerged as a prominent solution, meeting these requirements and playing a crucial role in the design of digital circuits. This paper concentrates on the design, implementation, and performance evaluation of gates (AND, OR, XOR) and combinational circuits (2 × 1 Mux, subtractor, and adder) based on VgSOT MTJ. The performance characteristics of these circuits have been evaluated, and comprehensive comparisons have been made with conventional 45 nm CMOS and SOT/STT-based circuit designs. In this paper, based on the latency performance analysis, it has been established that the logic gates and combinational circuits utilizing VgSOT exhibit superior performance of approximately 96% compared to CMOS counterparts. Similarly, latency performance improvement of 44% and 75%, respectively, is seen for VgSOT-based logic gates and combinational circuits over SOT and STT-based circuits. Analysis of power consumption in VgSOT-based logic gates has revealed a remarkable power efficiency of ∼98% over CMOS- and SOT/STT-based circuit implementations. Implementing VgSOT MTJ-based design brings about notable improvements in the performance of different logic designs.

Accelerating Conjugate Heat Transfer Simulations in Squared Heated Cavities through Graphics Processing Unit (GPU) Computing

This research develops an innovative framework for accelerating Conjugate Heat Transfer (CHT) simulations within squared heated cavities through the application of Graphics Processing Units (GPUs). Although leveraging GPUs for computational speed improvements is well recognized, this study distinguishes itself by formulating a tailored optimization strategy utilizing the CUDA-C programming language. This approach is specifically designed to tackle the inherent challenges of modeling squared cavity configurations in thermal simulations. Comparative performance evaluations reveal that our GPU-accelerated framework reduces computation times by up to 99.7% relative to traditional mono-core CPU processing. More importantly, it demonstrates an increase in accuracy in heat transfer predictions compared to existing CPU-based models. These results highlight not only the technical feasibility but also the substantial enhancements in simulation efficiency and accuracy, which are crucial for critical engineering applications such as aerospace component design, electronic device cooling, and energy system optimization. By advancing GPU computational techniques, this work contributes significantly to the field of thermal management, offering a potential for broader application and paving the way for more efficient, sustainable engineering solutions.

Do socially responsible indices outperform conventional indices? Evidence from before and after the onset of Covid‐19

AbstractAccording to traditional portfolio theories constraints, restrictions, and screens applied in portfolio selection reduces the diversification opportunities which can impact financial performance. Is this true in the case of socially responsible investment (SRI)? To answer this, present study analyzes the performance of Socially Responsible (SR) indices in comparison to conventional indices in an emerging economy. The uniqueness of the study is that it analyses the performance of Shariah, ESG, and thematic indices in a single study. Further, the study measures the impact of Covid‐19 on them. Comparative performance evaluation was conducted by using absolute return analysis and risk‐adjusted measures namely, Sharpe ratio, Treynor ratio, tracking error, information ratio, capital asset pricing model (CAPM), Fama–French three‐factor, and Carhart's four‐factor models. The Structural break was identified, hence analysis was conducted for the total period (January 2017–March 2023) and two sub‐periods, that is, pre and post‐Covid‐19 period. No significant difference was found between the returns of SR indices and conventional indices as against the benchmark index on the basis of absolute return analysis. Sharpe ratio and Treynor ratio both were having negative values for all the SR and conventional indices. Tracking error for all the SR and Conventional indices were very low. The CAPM and both multi‐factor models univocally pointed toward the underperformance of all the SR (except S&amp;P BSE 100 ESG index which had equal performance) and both conventional indices against the benchmark index. Noteworthy point is that only Shariah indices gave the highest returns during post‐Covid period. This research will help in deepening the SRI in the capital market. Companies should increase their ESG scores and make efforts to be listed on the SR indices. Policymakers should announce some kind of rebates, or recognition for star‐performing companies in the field of sustainability to encourage other companies to adopt SR practices in their business operations. The novelty of the current study is that it adds to the socially responsible literature by analyzing the performance of Shariah, ESG, and Thematic indices and conventional indices in a single study in the fastest‐growing economy of India and analyses the impact of Covid‐19 on this performance.

Comparative Evaluation of Dissolution Performance in a USP 2 Setup and Alternative Stirrers and Vessel Designs: A Systematic Computational Investigation.

The dissolution testing method described in the United States Pharmacopeia (USP) Chapter ⟨711⟩ is widely used for assessing the release of active pharmaceutical ingredients from solid dosage forms. However, extensive use over the years has revealed certain issues, including high experimental intervariability observed in specific formulations and the settling of particles in the dead zone of the vessel. To address these concerns and gain a comprehensive understanding of the hydrodynamic conditions within the USP 2 apparatus, computational fluid dynamic simulations have been employed in this study. The base design employed in this study is the 900 mL USP 2 vessel along with a paddle stirrer at a 50 rpm rotational speed. Additionally, alternative stirrer designs, including the hydrofoil, pitched blade, and Rushton impeller, are investigated. A comparison is also made between a flat-bottom tank and the USP round-bottom vessel of the same volume and diameter. Furthermore, this work examines the impact of various parameters, such as clearance distance (distance between the bottom of the impeller and bottom of the vessel), number of impeller blades, impeller diameter, and impeller attachment angle. The volume-average shear rate (Stv), fluid velocity (Utv), and energy dissipation rates (ϵtv) represent the key properties evaluated in this study. Comparing the USP2 design and systems with the same stirrer but flat-bottom vessel reveals more homogeneous mixing compared to the USP2 design. Analyzing fluid flow streamlines in different designs demonstrates that hydrofoil stirrers generate more suspension or upward movement of fluid compared to paddle stirrers. Therefore, when impellers are of a similar size, hydrofoil designs generate higher fluid velocities in the coning area. Furthermore, the angle of blade attachment to the hub influences the fluid velocity in the coning area in a way that the 60° angle design generates more suspension than the 45° angle design. The findings indicate that the paddle stirrer design leads to a heterogeneous shear rate and velocity distributions within the vessel compared with the other designs, suggesting suboptimal performance. These insights provide valuable guidance for the development of improved in vitro dissolution testing devices, emphasizing the importance of optimized design considerations to minimize hydrodynamic variability, enhance dissolution characterization, and reduce variability in dissolution test results. Ultimately, such advancements hold potential for improving in vitro-in vivo correlations in drug development.

Comparative performance evaluation of four different methods for diagnosing Giardia infection in dogs and zoonotic assemblages’ identification

Giardia duodenalis (syn. G. intestinalis or G. lamblia) is a parasitic protozoan that infects the upper intestinal tract of a broad range of hosts, including humans and domestic animals. Thus, it has raised concerns about the public health risk due to companion animals. Recently, with the improvement of living standards and increasing contacts between pets and humans, the zoonotic transmission of Giardia has dramatically increased. From a genetic point of view, G. duodenalis should be viewed as a complex species that includes eight different species-specific genetic assemblages. The laboratory diagnosis is mainly based on the finding of microscopic cysts in stool samples by coprological examination. Other methods include the detection of antigens, immunoassays or PCR protocols, which allow the identification of Giardia assemblages. The study aimed to compare the performance of Direct Fluorescence Antibody test (DFA), zinc sulfate flotation technique (ZnSO4), rapid diagnostic test (RDT), end-point PCR amplification (PCR) for the detection of Giardia and to identify the concerning assemblages in a canine population from Central Italy. Direct fluorescence antibody test is the reference standard for laboratory diagnosis of Giardia in fecal samples from dogs, despite the microscopic examination after flotation remains the most useful method in many veterinary diagnostic centers. The present findings demonstrate the high performance of DFA and ZnSO4 in detecting Giardia, while RDT may be useful as alternative or complementary method to the DFA and ZnSO4. PCR performance was low, but it allowed determining Giardia B zoonotic assemblage in 25% of the PCR-positive specimens (15 out of 60), while the remaining PCR-positive isolates belonged to the dog-specific assemblage C. The 26% prevalence of G. duodenalis detected by DFA in owned dogs and the identification of potentially zoonotic assemblages underline the potential risk for public health and indicate frequent cross-species transmission of the parasite between humans and dogs.