Common Formula Research Articles

DIA-based serum proteomics revealed the protective effect of modified siwu decoction against hypobaric hypoxia

Ethnopharmacological relevanceSiwu decoction (SWD) is a common traditional formula for nourishing blood, and its derived formulas are also widely used in traditional Chinese medicine (TCM) clinic. However, the protective effects of SWD and its derived formulas on blood deficiency and blood stasis caused by rushing to the plateau are rarely reported, and the underlying mechanism has not been fully elucidated. Aim of the studyThis study explores the pharmacological effects and mechanisms of modified siwu decoction (MSWD) adding Persicae Semans (Prunus persica (L.) Batsch) and Carthami Flos (Carthamus tinctorius L.) against hypobaric hypoxia (HH). The acute toxicity of MSWD was also evaluated to further validate the potential of MSWD as a therapeutic candidate for HH. Materials and methodsHypoxic models of C57BL/6 J and KM male mice were used to evaluate the pharmacological effect of MSWD. 2 μL serum sample of C57BL/6 J mice was digested into peptide mixtures and analyzed with DIA mode on an Orbitrap Fusion Lumos mass spectrometer after LC separation. The peptide and protein identifications were limited to a 1% FDR. Screening of differential expressed proteins, correlation analysis, hierarchical clustering analysis, principal components analysis and Mfuzz analysis were all performed by R packages. The protein−protein interaction network was analyzed using the STRING website and constructed with Cytoscape software. ResultsMSWD showed a protective effect against acute hypoxia exposure through increasing the number of red blood cells and improving hemodynamics indexes in mice. Meanwhile, the biochemical results showed that MSWD could reduce the inflammation and oxidative stress, reduce the content of organ injury biomarkers and significantly improve the high-intensity exercise ability of mice. Subsequently, serum DIA proteomic results revealed significant changes in proteomic characteristics after MSWD intervention. Specifically, proteins related to oxidative stress and ubiquitin-proteasome system, such as Sod1, Gstp1, Vcp and Usp14, were down-regulated after MSWD intervention, suggesting that the protective effect of MSWD involved the reduction of oxidative stress and energy expenditure. MSWD also intervened in energy metabolism and lipid metabolism processes by altering the expression levels of Eno1, Sphk1 and Apoa1 to ameliorate hypoxia-induced disorders. At the same time, MSWD acute toxicity test showed no obvious toxicity. ConclusionsMSWD has a good protective effect against HH by ameliorating hypoxia-induced disorders of energy and lipid metabolism, supporting MSWD as a safe drug candidate for the prevention and treatment of acute hypoxia fatigue.

A common recursive formula for Ramanujan’s ₁𝜓₁ and Bailey’s very-well-poised ₆𝜓₆ summation formulas

In this paper, we establish a general recursive formula via the usual telescoping method for certain bilateral truncated sum which may serve as a common source for Ramanujan’s 1 ψ 1 {}_1\psi _1 , Bailey’s very-well-poised 6 ψ 6 {}_6\psi _6 , and Rogers’ 6 ϕ 5 {}_6\phi _5 summation formulas. The corresponding derivations for these summation formulas are presented in details.

Tetravalent Terbium Chelates: Stability Enhancement and Property Tuning.

Coordination chemistry of rare-earth elements has been dominated by the +3 oxidation state. Complexes with higher-valence lanthanide ions are synthetically challenging but are of fundamental research interest and significance as advanced molecular materials. Herein, four tetravalent terbium complexes (2-5) of the common formula [Tb(OSiPh3)4L] (L = ethylene glycol dimethyl ether (DME), 2,2'-bipyridine (bpy), 2,2'-bipyrimidine (bpym), and 1,10-phenanthroline (phen)) are reported. Crystallographic analyses reveal in each of these complexes a hexacoordinate Tb(IV) ion situated in a distorted octahedral coordination environment formed by four triphenylsiloxido ligands and a bidentate chelating ligand. The use of chelating ligands enhances the stability of the resulting complexes over their THF solvate precursor. More significantly, the aromatic N-chelating ligands have been found to tune effectively the electronic structures of the complexes, as evidenced by the sizable potential shifts observed for the quasi-reversible redox Tb(IV/III) process and by the changes in their absorption spectra. The experimental findings are augmented with quantum theoretical calculations in which the ligand π-donation to the 5d orbitals of the Tb(IV) center is found to be primarily responsible for stability enhancement and the corresponding changes of physical properties observed. Magnetic measurements and results from electron paramagnetic resonance studies produced small absolute values of zero-field splittings of these complexes, ranging from 0.1071(22) to 1.1484(112) cm-1 and comparable to the values reported for analogous Tb(IV) complexes.

A study related to the treatment of gastric cancer with Xiang-Sha-Liu-Jun-Zi-Tang based on network analysis

PurposeXiang-Sha-Liu-Jun-Zi-Tang(XSLJZT) is a common formula for the treatment of Gastric Cancer(GC) and is widely used in clinical practice, however, there is a lack of investigation into its mechanism. MethodsWe collected and organized drug and disease targets, constructed the “XSLJZT-Active Ingredient-Target” visualization network, and performed GO and KEGG functional enrichment analysis of crossover genes, followed by molecular docking of active ingredients and core targets. The best docked monomers were combined with weighted gene co-expression network analysis(WGCNA) and macroscopically analyzed by GO and KEGG enrichment techniques. The results of cluster gene difference analysis, ROC evaluation, and CIBERSORT immune infiltration analysis were evaluated and finally supported by cellular experiments. ResultsThe main components of XSLJZT are quercetin, stigmasterol, and naringenin, effectively treat GC by targeting STAT3, TP53 and MAPK3, which are involved in IL-17, TNF and HIF-1 signaling pathways. The results of molecular docking showed that quercetin bound better to the core targets. We performed an in-depth analysis of this monomer and found that quercetin acts on the core targets of TP53, MMP9, TIMP1 and MYC, and is involved in two key signaling pathways, TNF and IL-17, thus effectively treating GC. The experimental results are consistent with our analysis that quercetin inhibits the proliferation of GC cells and promotes apoptosis, and TP53, MYC and TIMP1 are the quercetin targets for the treatment of GC. ConclusionThe present study tentatively suggests that quercetin, the main active ingredient in XSLJZT, can exert a therapeutic effect on GC by targeting TIMP1.

A Deep-Learning Approach to Spleen Volume Estimation in Patients with Gaucher Disease.

The enlargement of the liver and spleen (hepatosplenomegaly) is a common manifestation of Gaucher disease (GD). An accurate estimation of the liver and spleen volumes in patients with GD, using imaging tools such as magnetic resonance imaging (MRI), is crucial for the baseline assessment and monitoring of the response to treatment. A commonly used method in clinical practice to estimate the spleen volume is the employment of a formula that uses the measurements of the craniocaudal length, diameter, and thickness of the spleen in MRI. However, the inaccuracy of this formula is significant, which, in turn, emphasizes the need for a more precise and reliable alternative. To this end, we employed deep-learning techniques, to achieve a more accurate spleen segmentation and, subsequently, calculate the resulting spleen volume with higher accuracy on a testing set cohort of 20 patients with GD. Our results indicate that the mean error obtained using the deep-learning approach to spleen volume estimation is 3.6 ± 2.7%, which is significantly lower than the common formula approach, which resulted in a mean error of 13.9 ± 9.6%. These findings suggest that the integration of deep-learning methods into the clinical routine practice for spleen volume calculation could lead to improved diagnostic and monitoring outcomes.

Beyond views, productivity, and citations: measuring geopolitical differences of scientific impact in communication research

Scientometric analyses applying critical sociological frameworks have previously shown that high-prestige research output—with regards to both quantity and impact—is typically clustered in a few core countries and world regions, indicating uneven power relations and systematic biases within global academia. Although citation count is a common formula in these analyses, only a handful of studies investigated altmetrics (impact measures beyond citation-based metrics) in communication science. In this paper, we explore geopolitical biases of impact amongst the most productive scholars in the field of communication from 11 countries and 3 world regions. Drawing on SCOPUS data, we test three formulas that measure scholarly performance (citations per document; views per document; and citations per view) to investigate how geographical location affects the impact of scholars. Our results indicate a strong US-dominance with regard to citation-based impact, emphasizing a further need for de-Westernization within the field. Moreover, the analysis of altmetric formulas revealed that research published by Eastern European and Spanish scholars, although accessed similarly or even more often than American or Western European publications, is less cited than those. Country-level comparisons are also discussed.

Technology Transfer Assessment in Regional Business Contexts

This paper proposes a procedure for the organization of meetings between leaders of research groups and representatives of R&D&I departments in companies that is inspired by speed dating. The participating candidates were first assessed and selected based on their potential for an effective linkage in accordance with certain relevant variables taken from the literature on efficiency in technology and knowledge transfer activities. The process and findings of the application of this procedure were analyzed through the action research methodology in a real case, where 12 research groups and 55 companies participated in a pilot project fostered by the public authorities. Although meetings represent a common formula to promote collaboration between universities and industry, very few academic papers have analyzed the appropriate procedures for the organization of such meetings, which could help organizers not only to achieve the best interaction possible but also to improve the matching between a research group and the companies that are potentially interested in forming a stable collaboration for sustainable transfer activities. The results show that this procedure allows research groups to efficiently achieve first contact with companies in order to assess the potential for more lasting and sustainable collaboration.

Novel Zn- and Cd-Schiff base complexes as the potent anticancer agents: Synthesis, spectral characterization and theoretical study

A novel unsymmetrical bidentate Schiff base ligand and its complexes, with the common formula of MLX2 (M(II) = Zn(II) and Cd(II); X− = Cl−, Br− and I−), using a reaction between 1,2-diaminobenzene and (E)-3-(4-(Dimethylamino) phenyl) acrylaldehyde were successfully synthesized. The structure of all the as-made compounds was identified by techniques including 1H NMR,13C NMR, ESI-mass, FT-IR, UV–Vis, melting point and molar conductivity. The analytical and spectral data confirmed the metal to ligand ratio of 1:1 in the Zn(II) and Cd(II) complexes. The anticancer activity of the compounds on cancer cells was investigated on HCT116 cell line, where the MTT method was used to screen cytotoxicity effects. The flow cytometry method also was used to determine the mechanism of cell death. Our data reveal that CdLCl2 can block the cell cycle and induction apoptosis. In addition, using Gaussian 16 program, the structure of the samples was optimized, and some important properties such as enthalpy change, entropy change and total energy were extracted. The molecular electrostatic potential, nonlinear optical properties, natural bond orbital and frontier molecular orbitals analysis were calculated for all compounds. Further, the electrophilic and nucleophilic sites, energy gap, ionization potential, electron affinity, natural population analysis and natural atomic orbitals were studied.

Transient thrust behavior of low‐aspect‐ratio rocket propulsion systems**

AbstractIn this work, the transient thrust force of the solid propellant rocket motor is numerically computed to illustrate the shortcomings of the common thrust formula in transient periods of operation. The internal ballistics of the solid rocket motor is simulated using the quasi one‐dimensional governing equations in which the finite volume method and the Roe′s scheme are utilized in calculation procedure. This is demonstrated that in transient operation of the propulsion systems, the common thrust formula may ignore some parts of physical phenomena because it has been derived based on steady state assumption, but there are some moving waves in transient operation in the combustion chamber which induce the fluctuations on the thrust force. So, the thrust‐time history must be computed by pressure force integration over the whole solid boundaries of the motor at any instant. The results show that such transient thrust behavior is more intense in low aspect ratio propulsion systems like small space thrusters.

Network pharmacology and bioinformatics to identify molecular mechanisms and therapeutic targets of Ruyi Jinhuang Powder in the treatment of monkeypox.

Monkeypox outbreaks across the globe has aroused widespread concern. Ruyi Jinhuang Powder (RJP), a common formula in Chinese medicine, is used to treat pox-like illnesses. This study aimed to identify the molecular mechanisms and therapeutic targets of RJP for the treatment of monkeypox using network pharmacology and bioinformatics techniques. The bioactive substances and potential targets of each component of RJP were retrieved from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The differentially expressed genes (DEGs) of the monkeypox virus (MPXV) were identified from the GSE24125 by GEO2R. Key signaling pathways, bioactive components, and potential targets were obtained by bioinformatics analysis, including gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), disease ontology (DO), and protein-protein interactions (PPI) analyses. Finally, molecular docking was used to predict the interaction between active compounds and core targets. A total of 158 active ingredients and 17 drug-disease-shared targets of RJP were screened. Bioinformatics indicated that wogonin and quercetin might be potential drug candidates. Potential therapeutic targets were identified. Immune-related mechanisms that exerted antiviral effects included signaling pathways like TNF, age-rage, and c-type lectin receptor pathways. Our results illustrated the good therapeutic effect of RJP on monkeypox in terms of biological activity, potential targets, and molecular mechanism. This also offered a promising strategy to reveal the scientific basis and therapeutic mechanism of herbal formulas used to treat the disease.

Evolution of vertebral numbers in primates, with a focus on hominoids and the last common ancestor of hominins and panins

The primate vertebral column has been extensively studied, with a particular focus on hominoid primates and the last common ancestor of humans and chimpanzees. The number of vertebrae in hominoids—up to and including the last common ancestor of humans and chimpanzees—is subject to considerable debate. However, few formal ancestral state reconstructions exist, and none include a broad sample of primates or account for the correlated evolution of the vertebral column. Here, we conduct an ancestral state reconstruction using a model of evolution that accounts for both homeotic (changes of one type of vertebra to another) and meristic (addition or loss of a vertebra) changes. Our results suggest that ancestral primates were characterized by 29 precaudal vertebrae, with the most common formula being seven cervical, 13 thoracic, six lumbar, and three sacral vertebrae. Extant hominoids evolved tail loss and a reduced lumbar column via sacralization (homeotic transition at the last lumbar vertebra). Our results also indicate that the ancestral hylobatid had seven cervical, 13 thoracic, five lumbar, and four sacral vertebrae, and the ancestral hominid had seven cervical, 13 thoracic, four lumbar, and five sacral vertebrae. The last common ancestor of humans and chimpanzees likely either retained this ancestral hominid formula or was characterized by an additional sacral vertebra, possibly acquired through a homeotic shift at the sacrococcygeal border. Our results support the ‘short-back’ model of hominin vertebral evolution, which postulates that hominins evolved from an ancestor with an African ape–like numerical composition of the vertebral column.

Economic Damage Levels of the Green Shield Bug (Palomena prasina, Hemiptera: Pentatomidae) in Türkiye Hazelnut Orchards

The green shield bug (GSB) (Palomena prasina, Hemiptera: Pentatomidae) is one of the most important pests of hazelnut in Turkish hazelnut orchards. This harmful insect causes serious yield and quality losses by feeding directly on fruits every year. Their feeding on hazelnut fruits may result in nut abortion (empty nuts) in early season and cause shriveled and corked kernels in kernel expansion period. Insect pest management must be decided by depending on insect population level in field according to integrated pest management concept. Economic injury level (EIL) and economic threshold (ET) are the main essential points that must be considered in decision for insect pest control. Thus, it can be possible to protect the natural environment from unnecessary pesticide applications and the growers from high production costs. Therefore, determining of economic decision levels for controlling pests is critical. This is especially important for hazelnuts, which are grown on hundreds of thousands of hectares of land in Türkiye, and for the GSB, a serious pest that requires a couple of chemical applications per year. The economic decision levels vary mainly due to insect species and their damage potential, crop value in the market and control costs which can change over years and countries. This study aimed to calculate the EIL and ET values for GSB control action in hazelnut orchards in Türkiye using new economic market data. Based on previous research, the authors calculated the yield loss caused by one individual of GSB in this study. Direct yield loss, as well as quality and quantity losses from damaged kernels, were calculated separately and then totaled. The data, including crop value and control costs necessary for calculation was updated from free market sources. In the calculation of EIL/ET, the most common formula (EIL= C/ VxbxK) was used. As a result, the economic threshold for a single insecticide application was determined to be3.8 insect/da (=0.1 ha) for K=1 value and 4.76 insect/da for K=0.8 value, for single insecticide application. When ET values were converted in traditional Turkish approach that is special for hazelnut orchards; ET values for K=1 and K=0.8 were 0.76 insect/ 10 “hazelnut “ocak” (traditional growing of hazelnut plants together) and 0.95 insect/ 10 “ocak” respectively. If 2 applications per year for GSB were considered, ET values were doubled up and calculated to be 7.6 and 9.47 insect/da for K=1 and K=0.8 value respectively, and thusly 1.52 and 1.9 insect/ 10 “ocak” for Türkiye. For practical reasons, the ET value for GSB was recommended as 10 insect/da and 2 insects/10 “ocak”, for Türkiye, with consideration of 2 chemical applications and K= 0.8.

Exploring Human Milk, Nutrition, Growth, and Breastfeeding Rates at Discharge(HUMMINGBIRD Study): a protocol for a pilot randomised controlled trial

IntroductionMother’s own breast milk (MOM) is the optimal nutrition for preterm infants as it reduces the incidence of key neonatal morbidities and improves long-term outcomes. However, MOM shortfall is common...

Phenol-Croton Oil Chemical Peeling Induces Durable Improvement of Constitutional Periorbital Dark Circles.

Constitutional periorbital dark circles (PDC) are common in skin of color and represent a therapeutic challenge. To summarize the experience of the International Peeling Society on the safety and effectiveness of deep chemical peeling in the treatment of constitutional PDC. Multi-institutional, retrospective case series (1990-2020) of constitutional PDC treated by deep chemical peeling. Descriptive analysis by age, sex, Fitzpatrick phototype, phenol-croton formula, degree and durability of improvement, and complications. Fifty-five phenol-croton oil peels were performed in 52 patients: 3 patients received a second peel for periorbital rhytids 72 to 84 months after the first peel. 92% (48/52) of patients were women; the median age was 46 years (range, 23-68 years). 89% (46/52) of patients were Fitzpatrick III-IV. Most common formula included phenol 60% to 65% and croton oil 0.6% to 0.7%. 89% (49/55) of peels demonstrated >50% clinical improvement. The median duration of improvement was 24 months (range, 1.5-168 months), and 69% (36/52) of patients demonstrated ongoing improvement at the last follow-up. 4% (2/55) of peels exhibited complications of persistent erythema that resolved without scarring. Based on its safety and effectiveness, deep chemical peels are a treatment of choice for constitutional PDC.

The Deconstruction of Horror Film Formula in Midsommar by Ari Aster (2019)

The research entitled “The Deconstruction of Horror Film Formula in Midsommar by Ari Aster” aims to figure out the deconstruction of the horror film formula in Midsommar in contrast to horror film formula in general by Brigid Cherry. The researcher used a qualitative method to analyze the data. The primary data are taken from Ari Aster’s movie, Midsommar, in the form of cinematic codes such as setting, lighting, sound, and color. In addition, the researcher also used deconstruction theory in literature and cultural elements such as Scandinavian culture to analyze the deconstruction of horror film formulas in Midsommar film. The researcher found four binary oppositions of the horror film formula in Midsommar. The first one is the deconstruction of setting where Midsommar changes the common horror film formula setting of terrifying into peaceful. The second one is the deconstruction of lighting as Midsommar uses bright lighting instead of the common dark one. The third one is the deconstruction of sound as Midsommar uses dramatic sound instead of the common creepy one. Lastly, the deconstruction of color in Midsommar as it uses various colors instead of monotone ones that are commonly used in the horror film. This research illustrated the deconstruction of the horror film formula in the movie Midsommar from Brigid Cherry’s common original formula by reversing the non-dominant elements into becoming the dominant elements

New Modification of Polar Nonlinear Optical Iodate Fluoride PbF(IO3), the Family MX(IO3), M = Bi, Ba, Pb, X = O, F, (OH) Related to Aurivillius Phases and Similar Iodates

A new modification of PbF(IO3) has been obtained as single crystals from hydrothermal synthesis, alongside the known centrosymmetric, Pb(IO3)2, as a second phase. Measured with the Kurtz-Perry SHG method, the new crystals are phase-matchable for YAG:Nd laser radiation and demonstrate strong SHG output. According to an X-ray diffraction analysis conducted on a single crystal at low temperature, the new crystals appear monoclinic, of space group Pn, as opposed to the known orthorhombic modification of the PbF(IO3), of space group Iba2. The new crystals were also measured at room temperature, showing an orthorhombic disordered variant of the new phase (space group C2ma, standard Abm2). This variant presents an “average structure” with the mirror plane in the group. The low-temperature X-ray single-crystal experiment allowed us to find the correct structural model, where the mirror plane was found as a twin element in the real monoclinic Pn structure. A careful crystal chemical analysis led to a whole family of nonlinear optical crystals with a common formula, AX(IO3), A = Bi, Ba, Pb, X = O, F, (OH), currently counting six representatives, including the well-known BiO(IO3). All of them possess common central cationic layers similar to those known in Aurivillius-type phases, with anionic iodate layers attached above and below these layers instead of the perovskite-like, or halogens. The structure–property relationships are discussed with respect to the important role of the large cations: Pb2+, Bi3+ or Ba2+. A number of iodates with similar structures are also analyzed.

Synthesis, Characterization, Antimicrobial and Antifungal Activities of Mn(II) and Zn(II) Complexes with Azo Dye of 2-aminobenzimidazole and Indole

Azo dye namly 3-[2-(benzimidazolyl)azo]-indole (BIAI) derived from diazonium salt of 2-aminobenzimidazole and indole is reported and characterized based on IR, Proton nuclear magnetic resonanc (1H-NMR), elemental analyses, mass spectroscopy, X-ray diffraction (XRD) diffraction, electronic spectra. The BIAI was utilized to form Mn(II) and Zn(II) complexes. The complexes of BIAI ligands were identified via distinctive techniques (infrared, UV-vis, mass spectroscopies, molar conductance, Nuclear magnetic resonance (NMR) spectroscopy, X-ray diffraction (XRD) diffraction and magnetic sensitivity). The Mn(II) and Zn(II) complexes were characterized as octahedral complexes and non-electrolytic under the common formula [M(BIAI)2Cl2]. The BIAI and its complexes showed interesting antimicrobial activity against two sorts of bacterial strains, Staphylococcus aureus, Escherichia coli and Aspergillus niger fungi. The metal chelates of the BIPA ligand are non-electrolytes, according to molar conductance data. BIAI is coordinated to metal ions as a bidentate manner, according to IR spectra, with NN donor atoms of azomethine–N and methoxy–O. The BIPA ligand exhibited important chromic properties under pH changes.

One‐pot synthesis of 1,3‐disubstituted imidazo[1,5‐a]pyridines via acceptorless dehydrogenative coupling of primary alcohols promoted by binuclear ruthenium(II) N^O‐chelating complexes

A simple, straightforward and atom economic strategy to the synthesis of 1,3‐disubstituted imidazo[1,5‐a]pyridines using binuclear ruthenium(II) catalyst through acceptorless dehydrogenative coupling of primary alcohols with 2‐benzoylpyridine has been presented. New binuclear arene Ru(II) N^O chelating complexes of common formula [(η6‐benzene)2Ru2Cl2(μ‐L)], L = bis (furoylhydrazone) derivatives (1–3) were synthesized and characterized by analytical and spectral (FT‐IR, NMR and HR‐MS) techniques. The solid‐state molecular structure of one of the complexes (complex 1) has been authorized by single‐crystal XRD method. Further, a wide range of 1,3‐disubstituted imidazo[1,5‐a]pyridine derivatives have been successfully attained in good to excellent yields using binuclear arene Ru(II) catalysts with 1.0 mol% of catalyst loading. Further, control experiments unveil that the dehydrogenative coupling reaction mechanism proceeds via the formation of aldehyde and imine intermediates. The overall catalytic system releases H2 and water as only valuable by‐products.

Assessment of Wave Energy Resources in China

The evolution of renewable energy technologies may surmount fossil fuel disadvantages. Wave energy is considered one of the best alternatives to fossil energy due to its advantages. The 40-year (1979–2018) spatio-temporal distribution of wave energy is presented using European Centre for Medium-Range Weather Forecasts (ECMWF) Reanalysis v5 (ERA5) datasets. In addition, the common formula used for wave energy evaluation is derived from the linear wave theory in deep water, which is not applicable in shallow water. Therefore, a new equation that is suitable for both shallow and deep water is derived, which improves the accuracy of the wave energy evaluation for various water depths. The main aim is to investigate the spatio-temporal wave energy for offshore China from 1979 to 2018 and combine the new standard of classification to recommend the optimal area. The nearshore zone from Zhejiang province to Guandong province is considered the ideal zone, and the average annual wave energy density in this area is above 10 kW/m.

Influence of various spinel materials supported Ni catalysts on thermocatalytic decomposition of methane for the production of COx-free hydrogen

The thermocatalytic decomposition of methane is a promising method to produce COx-free hydrogen. The spinel powders with the common formula of AB2O4 and unparalleled features were widely used as a support of heterogeneous catalysts for the diverse catalytic process. In this research, the spinel powders containing the oxides of magnesium, calcium, barium, strontium, iron, and zinc were synthesized based on the solid-state mechanochemical preparation method, and the samples were used as catalyst support for the nickel-based catalysts in the thermal decomposition of methane for the production of COx-free hydrogen. XRD, BET, CO2-TPD, H2-TPR, TPO, and FESEM techniques were carried out to identify the physicochemical characteristics of the fresh and spent samples. The results showed that all samples have a nanocrystalline structure with excellent properties. The results demonstrated that the FeAl2O4-supported nickel catalyst presented the highest stability among the studied catalysts. The decline in the initial CH4 conversion was about 21% at 575 °C during the 300 min time on stream. However, other samples were completely deactivated during the stability test, which was related to the deposition and growth of the carbon nanofiber that covers the available catalyst active sites. The effect of the GHSV value (40000–60,000 ml.h−1.g−1cat), CH4:N2 (10:90–30:70), and reduction temperature (600–800 °C) were also investigated on the catalytic performance and rate of the deposited carbon of the 40 wt% NiO/FeAl2O4.