Common Cause Of Abortion Research Articles

Rapid diagnosis of the most common causes of abortion in Awassi ewes in Nineveh Governorate, Iraq

The study aimed to determine the rates of infection with the most common causes of abortion by Rapid methods of diagnosis, and abortion rates in the governorate’s areas. Rapid methods of diagnosis are important to help the veterinarian in diagnosing abortions in order to reduce the economic loss of sheep by taking the appropriate decision and treatment within a short time. This study was conducted on 92 samples (46 vaginal swabs, 46 blood) representing 60 flocks, the study was conducted from August 2022 to January 2023. The following tests were used, FASTest CHLAM Ag rapid screening test for Chlamydia, Rose Bengal Agglutination Test for Brucella, and Latex agglutination test for Toxoplasma. Results indicate that 13/46 positive samples were 28.26% for chlamydia, 29/46 positive samples 63.04% for Brucella, and 17/46 positive samples 36.95% for toxoplasma. It has been concluded that with a high infection rate of Chlamydia abortus in ewes in Nineveh, the rapid test was ideal for the diagnosis of Chlamydia. A high infection rate of Chlamydia in the center and northern areas of Nineveh.

Diagnosis of imminent placentitis in mares

Pregnancy loss and neonatal mortality have a significant economic impact on the equine industry. Placentitis is a common cause of abortion, stillbirths, and neonatal infections. Early diagnosis of placentitis allows prompt treatment and increases the chances of a pregnancy reaching term. Our study was designed to identify blood parameters that are diagnostic of imminent placentitis under natural conditions. We hypothesized that differences from physiological parameters of a healthy pregnancy indicate imminent placentitis. Blood sample collections and reproductive exams were prospectively performed, monthly, between 180 to 320 days of gestation, in a cohort of mares from 2 large broodmare herds in Lexington, KY. Twelve mares were diagnosed with placentitis based on rectal ultrasonographic examination (opening at the cervix, thickened placenta, fluid collection, placental edema, and/or placental separation). Blood samples collected on the day of placentitis diagnosis were designated as early onset, and the blood samples collected in the previous reproductive exam with no ultrasonographic abnormalities of the fetoplacental unit were named as pre-onset (before placentitis diagnosis). In addition, 12 gestion and age-matched control mares carrying healthy pregnancies based on the same parameters were paired to the mares with placentitis. We measured several immunological, inflammatory, and hormonal parameters (Table 1). Values were compared between groups within the same timepoint (placentitis vs control) and between timepoints (pre-onset vs early onset) using Paired Student's T test and Mann-Whitney U test, and significance set at p < 0.05. According to the gestational age, the interval between pre-onset timepoint and the diagnosis of placentitis (early onset) was around 40 days (227±6 days and 267±5 days, respectively).No differences were found between groups and timepoints for immunological and inflammatory parameters in the maternal circulation, including serum amyloid A (SAA) and alpha-fetoprotein (AFP). Both proteins have been shown to markedly increase in maternal circulation after experimental induction of placentitis. In the hormonal analyses, no differences were found in plasma progesterone levels and estradiol-17β/progesterone ratio. Plasma estradiol-17β concentration approached statistical difference (p=0.08) in mares at early onset of placentitis (408±35 pg/mL) compared to the control group (498±35 pg/mL). In conclusion, our data showed that early events of spontaneous placentitis do not increase inflammatory and immunological parameters in the maternal circulation. In addition, plasma estradiol-17β may be further investigated as an early indicator of placental dysfunction in mares with naturally occurring placentitis. Acknowledgments: We thank the Harry M. Zweig Memorial Fund for Equine Research for supporting our study.

Serological survey of Chlamydia abortus in Greek dairy sheep flocks

Chlamydiosis due to Chlamydia abortus is one of the most common causes of abortion in small ruminant flocks worldwide. Although the causative agent is zoonotic, chlamydiosis is not a reportable disease. There is lack of recent data concerning sheep chlamydiosis in Greece.In the current study, a serological investigation for Chlamydia abortus was conducted. Blood samples from 26 randomly selected sheep flocks not vaccinated against chlamydiosis were collected. From each flock, 15 to 20 blood samples were taken from adult female sheep. In total 464 blood samples were examined. One hundred and six samples were positive (22.8%), while 24 samples (5.24%) were doubtful. Moreover, at farm level, in 18 out of the total number of 26 farms, at least one positive animal was detected (69.2%).Chlamydiosis is considered a highly likely cause of sheep abortion in Greece. Therefore, vaccination of the sheep flocks is strongly recommended for the prevention and control of the disease.

Vaginal misoprostol vs intramuscular carboprost in first trimester abortion

Background: A number of conditions demands first trimester abortions including medical and social causes. This study was mainly undertaken in order to compare the oral misoprostol with the intramuscular carboprost for cervical ripening in suction evacuation for medical termination of pregnancy up to 12 weeks of gestation. Material and methods: A randomized controlled trial was undertaken to determine the safety and efficacy of misoprostol over carboprost in first trimester abortions for cervical ripening. A total 120 pregnant women were randomized in to two equal groups where one group received oral misoprostol and another group received intramuscular carboprost. Two groups were studied and compared for the baseline characteristics, induction abortion interval, cervical dilation, blood loss and complications. Results: The mean age of misoprostol group was 26.2 years and carboprost group was 25.8 years. There was no statistically significant difference in marital status, gravidity, parity and gestational age in this study. Incomplete abortion was the common cause of abortion in both the groups. The induction abortion interval was 195.2 minutes in misoprostol group and 89.9 minutes in carboprost group. The mean cervical dilation was 8.35 minutes in misoprostol group and 9.31 minutes in carboprost group.

Complete genome sequencing of a Tequintavirus bacteriophage with a broad host range against Salmonella Abortus equi isolates from donkeys.

Salmonella enterica subspecies enterica serovar abortus equi (S. Abortus equi) is the most common cause of abortion in mares. It has recently been found to cause abortion in donkeys more frequently in China. A novel virulent bacteriophage vB_SabS_Sds2 (hereafter designated as Sds2) was isolated from the feces of donkeys using a S. Abortus equi strain as a host. Phage Sds2 had an isometric polyhedral head and an uncontracted long tail, belonging to the Tequintavirus, Markadamsvirinae, Demerecviridae, Caudovirales. The genome of phage Sds2 was 114,770 bp, with a GC content of 40.26%. The genome contained 160 open reading frames (ORFs), and no ORFs were associated with pathogenicity, drug resistance, or lysogenization by sequence analysis. Both genome annotation and phylogenetic analysis indicated that phage Sds2 was highly similar to T5-like bacteriophages. Phage Sds2 could lyse 100% (30/30) of S. Abortus equi strains, 25.3% (24/95) of other serotypes of Salmonella strains, and 27.6% (8/29) of Escherichia coli strains using the double-layer agar plate method. The in vitro test showed that phage Sds2 had high bactericidal activity against S. Abortus equi at a wide range of MOIs. The in vivo test indicated that phage Sds2 had an inhibitory effect on abortion in mice challenged with S. Abortus equi. In general, phage Sds2 is a novel lytic phage with a wide host range and has the potential to prevent abortion caused by S. Abortus equi.

Causes of Different Types of Abortion in Women Referring To Educational and Medical Centers in Shiraz, Iran

Background & aim: Abortion in Iran, like some countries in the world, has long been a culturally, socially, politically and religiously sensitive issue. So the present study was conducted to investigate the causes of abortions in pregnant women referring to Shiraz educational and medical centers.Methods: : In this cross-sectional study, 437 woman who had an abortion, were selected by examining 5176 pregnant women using sequential sampling method during 4 months from educational and forensic medicine centers in Shiraz, Iran, in 2017. A self-structured questionnaire including demographic characteristics and obstetric data was completed for each mother. Data analysis was carried out using descriptive statistics.Results: Out of 88 abortions with a legal permission from the forensic medicine, 9.1% were due to maternal illness and 90.9% were due to fetal disease, the most common cause of which was fetal cranial and cerebral disorders (17.1%). Out of 105 cases of spontaneous abortion, 8 cases were due to maternal cause (the most common cause of uterine defects (4.8%) and 97 cases were due to fetal cause. Out of 244 induced abortions with obstetric indication, the most common cause was missed abortion (32%).Conclusion: In all three types of abortion, fetal causes were the most common cause of abortion. It seems that education, access to information and awareness raising activities involving the whole community will have a significant effect on preventing the occurrence of abnormalities. Emphasis is placed on the importance of conducting prenatal screening before 20 weeks of gestation.

Sheep abortion – a roundtable discussion

Foreword Abortion takes a heavy toll on the sheep industry, not only economically, but in emotional terms too. Enzootic abortion in ewes is consistently the most frequent diagnosis followed in descending order by infection with Toxoplasma gondii, Campylobacter spp., Salmonella spp. and Listeria spp. Causes of abortion also lead to increased barren rates, and the birth of weak lambs, so the impact of these pathogens is greater than just abortion. Fortunately there are vaccines for the two most common causes of abortion, providing farmers and the veterinary surgeons advising them with effective and sustainable tools in the control of enzootic abortion and toxoplasmosis. The uptake of these vaccines, however, remains lower than might reasonably be expected. In this roundtable discussion the barriers to the implementation of the extant measures of abortion control were discussed and suggestions made as to how abortion can better be prevented.

Seroprevalence of brucellosis and chlamydiosis in sheep and goats with history of abortion in Iran

• Brucellosis and chlamydiosis are common causes of abortion in livestock. • The prevalence of brucellosis was significantly higher in goats compared to sheep. • Inversely, goats showed a lower prevalence of chlamydiosis compared to sheep. • Brucella melitensis biovars 1 was identified in sheep and goats aborted fetuses. Abortion in small ruminants is a critical issue in many developing countries including Iran and causes devastating economic losses in small ruminant industry. The aim of this study was to evaluate the seroprevalence of brucellosis and chlamydiosis in sheep and goats with a history of abortion during lambing seasons in Iran. Samples were collected during 2019 from sheep and goat flocks located in Fars province (Southern Iran). A total of 124 sera (101 sheep and 23 goats) from aborted females as well as 29 aborted fetuses were sampled and analyzed for Chlamydia abortus and Brucella spp. using indirect enzyme-linked immunosorbent assay (i-ELISA) for chlamydiosis and a set of serological (RBT, SAT and 2-ME and i-ELISA) and bacterial tests for brucellosis. Seropositivity to C. abortus was also detected in 21.7 % and 4.3 % of sheep and goats, respectively. Brucella specific antibodies were found in 29.1 %, 22.6 %, 20.9 % and 23.4 % of tested sheep and goats using RBT, SAT,2-ME and i-ELISA, respectively. Bacterial isolation and biotyping led to the characterization of B. melitensis biovars 1 in sheep and goats aborted fetuses of Fars province. High prevalence of infection associated with brucellosis and chlamydiosis in both sheep and goat with a history of abortion highlights risks of human zoonotic infections by these pathogens. Therefore, special attention is required for further study and control program to counteract these infections in animals and humans.

In Silico Investigation of Conserved miRNAs and Their Targets From the Expressed Sequence Tags in Neospora Caninum Genome.

Neospora caninum is a protozoan parasite, the etiologic agent of Neosporosis—a common cause of abortion in cattle worldwide. Herd level prevalence of Neosporosis could be as high as 90%. However, there is no approved treatment and vaccines available for Neosporosis. MicroRNA (miRNA) based prophylaxis and therapeutics could be options for Neosporosis in cattle and other animals. The current study aimed to investigate the genome of Neospora caninum to identify and characterize the conserved miRNAs through Expressed Sequence Tags (ESTs) dependent homology search. A total of 1,041 mature miRNAs of reference organisms were employed against 336 non-redundant ESTs available in the genome of Neospora caninum. The study predicted one putative miRNA “nca-miR-9388-5p” of 19 nucleotides with MFEI value -1.51 kcal/mol and (A + U) content% 72.94% corresponding with its pre-miRNA. A comprehensive search for specific gene targets was performed and discovered 16 potential genes associated with different protozoal physiological functions. Significantly, the gene “Protein phosphatase” was found responsible for the virulence of Neospora caninum. The other genes were accounted for gene expression, vesicular transport, cell signaling, cell proliferation, DNA repair mechanism, and different developmental stages of the protozoon. Therefore, this study finding will provide pivotal information to future aspirants upon Bovine Neosporosis. It will also serve as the baseline information for further studies of the bioinformatics approach to identify other protozoal miRNAs.

Review on Major Protozoal Causes of Abortion in Cattle

Neospora caninum was organism described as parasite of domestic dog. The tissues cysts seen in neural tissues. The disease can be transmitted by vertical and horizontal. Trans-placental infected pubis: the typical presentation is a flaccid and hind limb paresis Neospora abortion tends to peaks at mid gestation. Serological positive calves will ultimately give birth to calves that are infective and seronegative. Seropositive cow produce less milk than seronegative cows. Neosparn caninum was shown to use cattle as intermediate host and dog as definitive hosts. The most recommendation for prevention is try to prevent fecal contamination of feed and water on the dairy. Trichomonas fetus found in the vagina, uterus, macerated fetus, prepuce, epididymis and vasdeferense. Bovine trichomoniasis is venereal disease manifest in the cow and heifers by infertility, abortion up to 5 ts month after breading pyometra, and fetal mummification. Therefore, some of the common causes of abortion on cattle are discussed in detail in this review. Keywords: abortion, cattle, trichomoniasis, diagnosis, neosporiasis DOI : 10.7176/JBAH/9-21-06 Publication date: November 30 th 2019

Histologically, immunohistochemically, ultrastructurally, and molecularly confirmed neosporosis abortion in an aborted equine fetus

Neosporosis is a common cause of abortion in cattle worldwide but is rare in horses. Here, the first case of histologically, ultrastructurally, immunohistochemically, and molecularly confirmed equine abortion caused by neosporosis is reported. Samples of lung, heart, liver, skeletal muscle, tongue, brain, and the placenta from a female fetus aborted at 280 days of gestation were fixed in formalin and submitted for diagnosis. Histologically, there was disseminated neosporosis with severe lesions in lungs, liver and the heart. Protozoal tachyzoites in all tissues reacted with polyclonal anti-Neospora caninum rabbit antibodies. Transmission electron microscopic observation on lung tissue revealed tachyzoites consistent with Neospora, including many rhoptries. Polymerase-chain reaction (PCR) using primers designed to amplify the rRNA gene internal transcribed spacer 1 (ITS1) of the Sarcocystidae was performed on DNA extracted from fetal tissues. Comparison of the ITS1 amplified from the foal tissue to sequences available in GenBank revealed 100% sequence identity to the ITS1 from three isolates of Neospora hughesi.

Detection of the presence of Coxiella burnetii in a case of goat abortion: a first report from India

Abortion is a major cause of economic loss to the goat industry. Coxiella burnetii the causative agent of Q fever is an important zoonotic agent known to be prevalent worldwide. In the present investigation, we detected the presence of Coxiella burnetii by the modified Ziehl Neelsen method of staining and its DNA by trans-PCR assay in the placenta obtained from the aborted goat. We also ruled out other common causes of abortion in this case. Based on a literature survey, this is the first report on the direct detection of Coxiella burnetii from an aborted goat to be reported from India.

Ascenderende placentitis bij de merrie

Een ascenderende infectie van de placenta wordt bij 3 tot 7% van de drachtige merries gezien. Het is een veel voorkomende oorzaak van abortus, premature geboorte en zwak geboren veulens (Troedsson, 2003; LeBlanc, 2010). Gezien de infectie opklimt vanuit de caudale geslachtstractus, zijn de eerste zichtbare en meest uitgesproken letsels te vinden ter hoogte van de caudale pool van het allantochorion waar dit tegen de baarmoedermond aan ligt. De symptomen zijn niet altijd even duidelijk of worden pas heel laat in het ziekteproces opgemerkt. Dit maakt een tijdige diagnose en initiatie van therapie uitermate moeilijk. Experimentele modellen om placentitis te bestuderen zijn moeilijk te managen en dubbel-blindexperimenten met controlegroepen zijn zeldzaam, waardoor het moeilijk is “evidence-based” advies te formuleren. In dit artikel wordt de diagnose, gebaseerd op de symptomatologie en het echografisch en endocrinologisch onderzoek, besproken en worden enkele therapeutische en prognostische aspecten benadrukt.

Sero-Detection of Cytomegalovirus and Rubella Virus IgG Antibodies Among Sudanese Pregnant Women in Khartoum State-Sudan

Abstract Background: Cytomegalovirus and Rubella virus are the most common causes of congenital infections, which increase morbidity and mortality at birth and one of the common causes of abortion in developing countries. Methodology: Plasma samples obtained from 87 pregnant womens, the samples were obtained from Omdurman Friendship Hospital 53(61%), AL-Saudi Specialized Hospital 21(25%) and Ultra lab Diagnostic Centre 13(14%).all samples were examined for presence of CMV and Rubella virus IgG antibodies by using an ELISA test. Results: The result showed that out of 87 blood samples investigated, 64(73.6%) were positive for CMV, while the reslt 23(26.4%) were negative and 85(97.7%) were positive for rubella, while the reslt 2(2.3%) were negative, where was 62(71.2%) samples had both CMV and Rubella virus IgG antibodies, 25(28.8%) had either CMV or Rubella virus IgG antibodies and there was no sample negative for both CMV and Rubella virus IgG antibodies. Conclusion: The present study observed the high prevalence rate of CMV and rubella virus IgG antibodies among pregnant women in Khartoum State. The level of infections is higher in pregnant women without history of miscarriage than those aborted women.

Chromosomal abnormalities of embryos as a major factor recurrent spontaneous abortion after artificial and natural fertilization

Chromosomal abnormalities are the most common causes of abortion in the first trimester. Most miscarriages in this term of pregnancy are due to aneuploidy, the frequency of which is 50–80%. Autosomal trisomy is the most common chromosomal abnormalities.The aim of the study was to investigate chromosomal abnormalities of abortive material in women with recurrent spontaneous abortion after in vitro fertilization and after natural fertilization.Cytogenetic and molecular cytogenetic studies include 440 samples of chorionic villi from women with recurrent miscarriage, among whom 240 patient had the pregnancy in IVF cycles (group I) and 200 women had a naturally way pregnancy (group II). The average age of patients was 33 years.Spectral karyotyping by fluorescent hybridization in situ on interphase nuclei of chorionic villi cells was performed with direct hybridization labeled with fluorescent dyes DNA probes.Results of cytogenetic and molecular cytogenetic studies showed that 43.33% abortions after IVF and 65.00% ones following natural conception had an abnormal karyotype, including autosomal trisomies respectively 35.00% and 42.00%; disomy X – 1.25% and 0.50%; monosomy X – 2.08% and 3.0%; monosomy 21 – 0.83% and 0.50%; polyploidy – 2.92% and 13.00%; structural chromosomal abnormalities – 0.83% and 4.0%; marker chromosome – 0.42% and 1.00%.Cytogenetic study of chorionic villi in recurrent spontaneous abortion is an integral part of the diagnostic evaluation. If at the cytogenetic study a spontaneous chromosomal anomaly detected, the next pregnancy proposed planning without a more detailed genetic examination. If a hereditary chromosomal anomaly, it is assessed the risk of its occurrence during the next pregnancy, carried out medical and genetic counseling.An artificial insemination with controlled selection of sperm and embryos in women with recurrent miscarriage history leads to a smaller number of chromosomal abnormalities in the embryo than in miscarriages after natural conception.

Aborto habitual. Factor infeccioso

El aborto habitual se define como la expulsión espontánea y consecutiva de por lo menos tres gestaciones de hasta 12 semanas de duración. Para que un agente infeccioso se pueda calificar de causante de aborto habitual, este debe permanecer vivo y virulento en el huésped, sintomáticamente o no, por un período lo suficientemente largo de tiempo como para lesionar a tres gestaciones en forma consecutiva.

Isolation of viable Neospora caninum from brains of wild gray wolves (Canis lupus)

Neospora caninum is a common cause of abortion in cattle worldwide. Canids, including the dog and the dingo (Canis familiaris), the coyote (Canis latrans), and the gray wolf (Canis lupus) are its definitive hosts that can excrete environmentally resistant oocysts in the environment, but also can act as intermediate hosts, harboring tissue stages of the parasite. In an attempt to isolate viable N. caninum from tissues of naturally infected wolves, brain and heart tissue from 109 wolves from Minnesota were bioassayed in mice. Viable N. caninum (NcWolfMn1, NcWolfMn2) was isolated from the brains of two wolves by bioassays in interferon gamma gene knockout mice. DNA obtained from culture-derived N. caninum tachyzoites of the two isolates were analyzed by N. caninum-specific Nc5 polymerase chain reaction and confirmed diagnosis. This is the first report of isolation of N. caninum from tissues of any wild canid host.

Diagnosis and control of small ruminant abortion

Abortion, as either an outbreak or enzootic disease issue, is an important production-limiting disease of sheep and goats. This article describes how to approach diagnosis and management of the more common causes of abortion in the US and Canada, i.e. Campylobacter fetus subspecies fetus, Campylobacter jejuni, Chlamydophila abortus, Toxoplasma gondii, and iodine deficiency goiter. It also gives an overview of the less-common causes of abortion in sheep and goats. Zoonotic implications of the various disease agents will also be addressed, as well as how to reduce risk to humans. Coxiella burnetii is covered in a separate lecture.

Detection and characterization of Leptospira spp. isolated from aborted bovine clinical samples

Leptospirais recognized as an important public health problem worldwide, especially in tropical countries, and is a common cause of abortion in dairy and beef herds. The aim of the present study was to detect and characterizeLeptospiraas the causative agent of abortion in cattle using a PCR-RFLP in Chaharmahal va Bakhtiari and Isfahan provinces, Iran. A total of 220 bovine aborted foetuses and 120 vaginal discharges from an aborted calf were collected from 64 commercial dairy herds. After isolation of 60Leptospiraspp. from samples, RFLP analysis was carried out withHindIII andHaeIII restriction enzymes in reference strains and isolated for characterization. In a total of 340 specimens, 46 (20.9%) and 14 (11.66%) were identified positive forLeptospiraspp. from aborted bovine foetuses and vaginal discharges, respectively. The present results also suggest thatL. interrogansserovarhardjohas the highest prevalence in the region under study andL. hardjois a major pathogen causing bovine abortion in Chaharmahal va Bakhtiari and Isfahan provinces of Iran.

Efficiency of a phase 1 vaccine for the reduction of vaginal Coxiella burnetii shedding in a clinically affected goat herd

The main route of human Q fever infections is inhalation of infectious aerosols. The bacterial agent, Coxiella burnetii, is frequently found throughout domestic ruminants. Females constitute potential shedders of C. burnetii through vaginal mucus, faeces and milk. Q fever is also a common cause of abortion, especially in goats. Massive load of bacteria is associated with placentas and aborted foetuses. In experimental conditions, the Coxevac inactivated phase 1 vaccine (CEVA Sante Animale, Libourne, France) was efficient, and dramatically reduced abortion and excretion of bacteria [1]. The aim of the present study was to evaluate the Coxevac vaccination impact on bacterial shedding in goats affected by Q fever in natural conditions.