In 1989,29 of 240 drylot Holstein cows from a New Mexico dairy aborted over a 5-month period. Seven of the 9 fetuses examined had multifocal necrotizing encephalitis and multifocal myocarditis. Neospora-like protozoa were found in tissues from 3 of the fetuses. More recently, a protozoan that reacted positively with anti-N. caninum serum was identified as the most common cause of abortion in California dairy cattle. 1,2 Many of these abortions were epizootic, with multiple abortions over a 1 or 2-month period. 1 Abortions and congenital infections in calves infected by a Neosporalike protozoan have been reported in the United States, but these were isolated cases involving only a single calf in each herd. This protozoan has never been isolated, so its exact identity remains unknown. Recent studies indicate that the organism is closely related to N. caninum, but some ultrastructural and antigenic differences exist between the bovine parasite and N. caninum isolated from dogs. Except for a single case of abortion in a beef cow from Maryland, the cases of fetal and congenital neosporosis previously reported from the United States are from the western states of New Mexico, 11 Califomia and Washington. Nineteen additional cases of bovine abortion associated with Neosporalike infection that were submitted to the South Dakota Animal Disease Research and Diagnostic Laboratory (SDADRDL) are herein reported. Specimens from cases of bovine abortions that are submitted to the SDADRDL undergo a routine protocol of testing to determine the cause of the abortion. Entire animals are necropsied and samples of brain, heart, lung, liver, kidney, placenta, tissues with gross lesions, and, since February 1991, skeletal muscle are fixed for histopathologic examination. Abomasal fluid is cultured for aerobic and microaerophilic bacteria and fungi. Scrapings of placenta are digested with potassium hydroxide and examined for fungal hyphae by a direct, nonspecific fluorescent antibody (FA) technique. If hyphae are found, the placenta is cultured for fungi. Impression smears of kidney are examined by a direct FA technique for 6 serovars of Leptospira. Direct FA tests are performed on frozen sections of kidney and spleen for infectious bovine rhinotracheitis (IBR) virus and bovine viral diarrhea (BVD) virus. Pools of brain, lung, liver, kidney, and spleen are homogenized and inoculated onto primary cultures of fetal bovine lung and turbinate cells for virus isolation. When received, a homogenate of placenta is inoculated by itself onto the same cell lines. Formalin-fixed paraffin-embedded
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