Colostrum Feeding Research Articles

PSV-7 Colostrum Intake Level Is Related to Level of Total Circulating Proteins and Essential Amino Acids

Abstract Colostrum intake potentiates piglet survival by transfer of immune factors. Additionally, colostrum also contains nutritional and bioactive molecules which could promote piglet’s growth and development. The objective of this study was to determine the effect of colostrum intake amount during the first 24h on plasma insulin, glucose, protein, and amino acid (AA) concentrations. Two female piglets were selected from 8 litters (n = 16) at birth and fed 10% (COL10) or 20% (COL20) colostrum based on birth weight over 12 bottle feedings occurring every 2h. Colostrum was collected from multiple sows and mixed to create a homogeneous pool. At birth and 24h, blood sample were collected, plasma separated and used to measure insulin, glucose, total protein, and AA concentrations. Analysis of the variance (PROC MIXED, SAS v.9.4) was used to evaluate significant (P≤0.05) differences between colostrum dose and time (birth vs. 24h). Colostrum intake did not influence plasma insulin at 24h (P=0.54). Glucose was higher at 24h (P< 0.001), but did not differ between COL10 and COL20 piglets (P=0.74). Total protein was greater at 24h (P< 0.001) as well as in COL20 compared to COL10 (P=0.006). Non-essential AAs (Ala, Asn, Asp, Cys, Gly and Glu) were reduced at 24h compared to birth (P< 0.03), while essential AAs (His, Ile, Met, Phe, Trp and Val) were increased after 24h of colostrum feeding (P< 0.05). At 24h, COL20 piglets had greater Ile and Met (P< 0.03) and tended (P=0.08) to have greater Leu and Phe. In conclusion, colostrum intake increases glycemia as well as total circulating proteins, and the dose of colostrum is related with greater circulation of total protein and essential AAs.

Use of Improved Production Technologies Among Goat Farmers in Abia State Nigeria

The study provided an empirical evidence on the use of improved goat production technologies among rural farmers in Abia State, Nigeria. The specific objectives of the study were, ascertain the extent of use of improved goat production technologies, determine factors influencing use of improved goat production technologies and identify constraints to access and use of improved goat production technologies. A multi-stage random sampling procedure was adopted in selecting 120 respondents. Data were collected through the use of questionnaire and analysed with frequency, percentage, mean and regression model. Respondents were aware of dipping (100%),deworming (99.2%) fostering of kids (93.3%) and colostrum feeding (71.7%), among others. On extent of use of improved goat production technologies, respondents highly used most of improved goat production technologies [grand mean = X̄ = 3.20]. All the respondents [100%] agreed that lack of access to credit was a constraint to use, 100% agreed on lack of credibility from source of technological information, 99.2% agreed that they were afraid of taking risk, 93.3% agreed on difficulty in technology application, among others. Age education, household size, farming experience, farm size, income and access to credit were the determinants of use of improved goat production technologies at 5% level of probability Greater use of available improved technologies will promote productivity, and therefore there is need for proper sensitization and awareness by relevant agencies. Again, credit should be made available to farmers by relevant governmental and nongovernmental agencies to increase the level of use of available improved technologies.
 Keywords: improved goat production technologies and farmers

Metabolic programming and monitoring tools in pre-weaned dairy calves

The current knowledge on the effects of nutritional and environmental factors in the late embryonic and neonatal phase of dairy calves on performance later in life is summarised. The importance of epigenetic factors is increasingly recognised in this context. Prolonged colostrum feeding, discouraged on most farms to prevent the spread of Johne's disease, can have beneficial effects on the calf's immune system and has been shown to give protection against rotavirus. The importance of colostrum hygiene for uptake of antibodies is explained. Intensive milk or milk replacer feeding leads to a more productive animal while reducing the rearing costs. Iron deficiency anaemia is a common condition in whole milk fed dairy calves, and monitoring haemoglobin and supplementing iron where necessary leads to higher growth rates in calves. Other monitoring tools like lung ultrasound scanning, and beta-hydroxy-butyrate testing at weaning are briefly introduced. In conclusion, the benefits of many early intervention measures are likely to be underestimated.

Rotavirus in Calves and Its Zoonotic Importance.

Rotavirus is a major pathogen responsible for diarrheal disease in calves, resulting in loss of productivity and economy of farmers. However, various facets of diarrheal disease caused by rotavirus in calves in the world are inadequately understood, considering that diarrheal disease caused by rotavirus is a vital health problem in calves that interrupts production benefits with reduced weight gain and increased mortality, and its potential for zoonotic spread. The pathological changes made by rotavirus are almost exclusively limited to the small intestine that leads to diarrhea. It is environmentally distributed worldwide and was extensively studied. Reassortment is one of the important mechanisms for generating genetic diversity of rotaviruses and eventually for viral evolution. So, the primary strategy is to reduce the burden of rotavirus infections by practicing early colostrum's feeding in newborn calves, using vaccine, and improving livestock management. Rotaviruses have a wide host range, infecting many animal species as well as humans. As it was found that certain animal rotavirus strains had antigenic similarities to some human strains, this may be an indication for an animal to play a role as a source of rotavirus infection in humans. Groups A to C have been shown to infect both humans and animals. The most commonly detected strains in both human and animals are G2, G3, G4, and G9, P [6]. Therefore, this review was made to get overview epidemiology status and zoonotic importance of bovine rotavirus.

Colostrum source and passive immunity transfer in dairy bull calves

Colostrum is essential for good neonate health; however, it is not known whether different calves absorb the nutrients from colostrum equally well. In this study, the absorption of protein, IgG, and γ-glutamyl transferase was compared in newborn dairy bull calves for 1 wk after feeding colostrum from different sources. Thirty-five Holstein-Friesian bull calves were randomly allocated into 3 groups and fed colostrum within 4 h after birth. Group A calves (n = 12) were bottle fed colostrum from their own dam for 3 d. Colostrum from these group A cows was also used as foster cow colostrum for the group B calves (n = 12), such that each group A and B calf pair received identical colostrum from each milking of the respective group A dam (10% of birth weight per day). The group C calves (n = 11) were fed 1 bottle (2 L) of pooled colostrum and transition milk (referred to as pooled colostrum), as was the standard practice on the dairy farm. The pooled colostrum was collected from the other dairy cows on the farm 0 to 4 d postpartum and stored at 4°C for less than 12 h. Blood was sampled from calves before the first feeding and at 1, 2, 3, and 7 d after birth. Levels of total solids, total protein, and IgG were higher in the dam colostrum than in the pooled colostrum. At birth, there were no differences between the calf groups for any measurements, and all calves had very low IgG levels. After receiving colostrum, the glucose, plasma γ-glutamyl transferase, serum total protein, and IgG concentrations increased significantly in all calves. There were no differences in any blood measurements at any time point between the pairs of group A and group B calves that received colostrum from the same cow except for the IgG concentration 2 d after birth. However, the group A calves had a higher total serum protein level and IgG concentration than the group C calves for all the time points after the first feeding. The group B calves had a higher IgG concentration than the group C calves on d 1, 2, and 7 after birth. Compared with groups A and B, there was no difference in the proportion of calves in group C that failed to have passive immunity transferred adequately based on the IgG threshold (<10 g/L). Thus, the calves receiving identical colostrum from the same cow had the same levels of IgG, and even the pooled colostrum provided sufficient transfer of IgG as the calves were fed within 4 h after birth.

FcRn is not the receptor mediating the transfer of serum IgG to colostrum in pigs.

In contrast to humans or rabbits, in which maternal IgG is transmitted to offspring prenatally via the placenta or the yolk sac, large domestic animals such as pigs, cows and sheep transmit IgG exclusively through colostrum feeding after delivery. The extremely high IgG content in colostrum is absorbed by newborns via the small intestine. Although it is widely accepted that the neonatal Fc receptor, FcRn, is the receptor mediating IgG transfer across both the placenta and small intestine, it remains unclear whether FcRn also mediates serum IgG transfer across the mammary barrier to colostrum/milk, especially in large domestic animals. In this study, using a FcRn knockout pig model generated with a CRISPR-Cas9-based approach, we clearly demonstrate that FcRn is not responsible for the IgG transfer from serum to colostrum in pigs, although like in other mammals, it is involved in IgG homeostasis and mediates IgG absorption in the small intestine of newborns.

Quantitative comparative phosphoproteomic analysis of the effects of colostrum and milk feeding on liver tissue of neonatal calves

Posttranslational modifications, mostly phosphorylation, are critical for protein structure and function. However, the association between liver phosphoproteins in neonatal calves and colostrum intake is not well understood. In this study, we examined the liver phosphoproteome profile in neonatal calves after receiving colostrum or milk. Liver tissue samples were collected from control calves (CON, n = 3) 2 h after birth and from calves that received colostrum (CG, n = 3) or milk (MG, n = 3) 24 h after birth. Hepatic phosphoprotein expression profiles were analyzed using quantitative proteomics based on the liquid chromatography-tandem mass spectrometry method. In total, 1,587 phosphorylated sites were identified in 1,011 liver proteins. The most abundant phosphorylation site AA was serine (87.5%), followed by threonine (11.9%) and tyrosine (0.5%). Among the 1,011 phosphoproteins, 219, 453, and 26 displayed differential expression in the CG versus MG, CG versus CON, and MG versus CON comparisons, respectively. Differentially expressed phosphoproteins in the CG-MG comparison included 3-phosphoinositide-dependent protein kinase 1, glucose transporter member 4, protein kinase N2, and vinculin, which were mainly involved in the glycogen metabolic process, transport, growth and development, and cell adhesion process, according to Gene Ontology analysis. Pathway analysis indicated their enrichment in the insulin signaling pathway, spliceosome, and adherens junction. The CG-CON comparison identified differentially expressed phosphoproteins and their target genes that were largely involved in the cellular process, macromolecule metabolic process, developmental process, and transport. Pathway analysis indicated their association with endocytosis, mechanistic target of rapamycin, AMP-activated protein kinase, and insulin signaling pathways. These data demonstrate that changes in the phosphoproteins of liver tissues may play an important role in energy metabolism and immune response in the calves that received colostrum. These results provide novel insights into the crucial roles of protein phosphorylation during the early life of newborn calves.

Delayed breast feeding initiation increases the odds of colostrum avoidance among mothers in Northwest Ethiopia: a community-based cross-sectional study

BackgroundColostrum is a yellowish and sticky breast milk produced in late pregnancy. Annually, 60% of 10.9 million under-five deaths globally are due to malnutrition. Of these, over two-thirds of the deaths are accounted by sub-optimal feeding practices in the first year of life, including colostrum discarding. However, evidence on the magnitude of colostrum avoidance and its associated factors at the community level is very limited in Ethiopia, particularly in the study area.Thus, this study aimed to assess the magnitude of colostrum avoidance and associated factors among mothers who gave birth in the last six months in Gozamen district, northwest Ethiopia, 2019.MethodsA community-based cross-sectional study was conducted among 741 (741) mothers who gave birth in the last six months in Gozamen district from August 1 to September 12, 2019. A stratified cluster sampling technique was used to select the study participants. Data were collected by face-to-face interviewer-administered, pretested, and semi-structured questionnaire. Binary logistic regressions (bi-variable and multivariable) were fitted to identify statistically significant variables. Adjusted Odds Ratio (AOR) with 95% Confidence Interval (CI) was used to declare statistically significant variables on the basis of p-value < 0.05 in the multivariable binary logistic regression.ResultsThis study indicated that the magnitude of colostrum avoidance was 22.1% (95% CI, 19.0, 25.2%).Mothers who did not get counseling on timely initiation of breast feeding (AOR = 3.91[95% CI, 1.98, 7.72]), not participate in pregnant woman forum (AOR = 2.59[95% CI, 1.30, 5.14]), initiate breast-feeding lately (more than 1 h) (AOR 2.27[95% CI, 1.18, 4.34]), and those having unfavorable attitude towards colostrum feeding (AOR = 7.35[95% CI, 3.89, 13.91]) were factors associated with the increased likelihood of colostrum avoidance. However, institutional delivery (AOR; 0.06[95% CI, 0.02, 0.19]) and prelacteal feeding (AOR; 0.10[95% CI, 0.05, 0.21]) were predictors associated with reduced likelihood of colostrum avoidance.ConclusionColostrum avoidance is a common practice in the study area. Therefore, in order to reduce this practice, strengthening infant feeding counseling, promoting institutional delivery, timely initiation of breastfeeding, health education, and community advocating are recommended interventions. In addition, creating awareness on the benefits of colostrum feeding is very instrumental to tackle the practice of colostrum avoidance.

Supplementary Bovine Colostrum Feedings to Formula-Fed Preterm Pigs Improve Gut Function and Reduce Necrotizing Enterocolitis.

Exclusive feeding with bovine colostrum (BC) protects preterm pigs against necrotizing enterocolitis (NEC) and BC has recently been tested as a supplement to a mother's own milk or formula (FOR) for very preterm infants. Using preterm pigs as a model for infants, we investigated if BC has gut- and NEC-protective effects at different proportions of the daily enteral intake given as BC. Sixty-eight caesarean-delivered preterm piglets (90% gestation) were allocated into four groups with increasing proportions of eight daily bolus feedings as BC: BC00 (only FOR feeding), BC25 (25% BC), BC50 (50% BC), or BC75 (75% BC). On day 5, the gut was collected for biochemical analyses. Body growth was increased in BC50 and BC75 piglets (2-fold, P < 0.05 vs BC00). The incidence of mild NEC-like lesions was similar among groups (67-86%), but BC75 reduced severe NEC-like lesions (27% vs 79% in BC00, P < 0.05). BC50 and BC75 improved hexose absorption and mucosal structure and reduced gut permeability (P < 0.05 vs BC00), while enzyme activities (lactase, aminopeptidase N and A, dipeptidyl peptidase IV) were improved in all pigs fed BC (P < 0.05). Across the measured variables, beneficial effects were most clear for the BC75 group, including reductions in colon tissue cytokine levels (interleukin 8, interleukin 1β, tumor necrosis factor α) and expression of immune- and apoptosis-related genes (LBP, TLR4, TLR2, IL8, STAT3, IL17, C3, all P < 0.05, relative to BC00). A proportion of 50-75% of daily enteral intake as BC is required to improve the intestinal structure, function, immunology, and NEC resistance in preterm piglets also fed formula. Further studies are required to show if and how supplementary BC may support gut development in preterm infants during the immediate postnatal period. It is challenging to translate results on optimal feeding regimens between species, and preterm infants would not receive a majority of their daily enteral intake as BC.

Survey on Colostrum Management by Dairy Farmers in the Netherlands.

Colostrum feeding is essential for the transfer of passive immunity and health of newborn calves. Information on current colostrum management practices to reduce calf morbidity and mortality is important but lacking for Dutch dairy herds. We therefore conducted a survey to investigate colostrum management strategies on Dutch dairy farms. The survey was specifically focused on the most recently born calf and was returned by 107 respondents (response rate of 13.4%). The mean amount of colostrum fed at first feeding was 2.9 liters. Overall, 79% of farmers provided the calf with at least 6 liters of colostrum in up to three feedings. The majority of respondents (84%) claimed to provide the calf with colostrum for the first time within 2 h post-partum. Using ordinal logistic regression and Wilcoxon rank sum test, we found no differences in time to first colostrum feeding or total amount of colostrum fed between bull calves and heifer calves, respectively. Ordinal logistic regression showed no significant differences in time to first colostrum feeding or time between calving and removing the calf from the dam between AMS and conventional milking herds. Two sample T-test comparing the total volume of colostrum showed no significant difference between AMS and conventional milking herds. Time of day at which a calf was born affected both volume fed at first colostrum feeding and time until first colostrum feeding. Calves born between 00.00 and 06.00 were significantly at risk of receiving the first colostrum later as compared to calves born at other times. Calves born in the evening received on average a lower amount of colostrum at first feeding. Survey results on colostrum management on most Dutch dairy farms are in agreement with the advice to feed as soon as possible after parturition and to provide at least 6 liters within 24 h of age. The current study points at time of calving as a potential risk factor for sub-optimal colostrum feeding. Further research is necessary to determine the consequences of this observation.

Breastfeeding Knowledge, Attitude and Practices of New-Mothers Presenting in Obstetrical Out-Patient Department

Background: Exclusive breastfeeding (EBF) with colostrum feeding improves the survival of the newborns. This varies with the time of initiation of breast-feeding, its duration and the age of weaning. Knowledge of breast feeding, attitude of mothers and their practices varies with many factors among populations. Objective: (1) To determine the prevalence of exclusive breastfeeding practice among mothers of infants 0–6 months of age presenting in obstetrical out-patient department. (2) To assess the level of knowledge, attitude and practices of new-mothers regarding breastfeeding presenting in obstetrical out-patient department. Study Design: Cross-sectional study. Settings: Department of Obstetrics &amp; Gynecology, Faisalabad Medical University, Faisalabad Pakistan. Duration: One month from August 01, 2020. Methodology: All new mothers of healthy infants 0–6 months old born between 37 and 42 gestation weeks attending the obstetrics OPD were included in the study. A detailed history was taken from all mothers. Parity, current age of infant, weight, height, BMI, BMI category, educational status, exclusive breastfeeding (EBF) and any pre-lacteal feed given, was noted. The independent variables for the study were maternal knowledge and attitudes towards EBF. 15 questions for assessment of knowledge and 12 for maternal attitude were used. Results: Among these 100 women, 66% (n=66) were currently feeding their newborns, but only 17% (n=17) of the total 100 women were practicing exclusive-breast-feeding. 95% were having good and above average knowledge regarding breast feeding practices. Still only half of the mothers had a positive attitude towards breast feeding; only 66% of the women were practicing breast feeding. Among educated mother 47.6% had a positive attitude toward breast feeding. Mothers with knowledge of breast-feeding above average/good were of 25 years or above age group. Conclusion: Education of the mothers can be linked to their high knowledge but is not significantly associated with their positive attitudes. Positive campaigns are required focusing not only un-educated young mothers, but also involving young educated primiparous women. Women should be encouraged from the first pregnancy regarding exclusive breast feeding and colostrum feeding, without the use of any pre-lacteal feeds, whatever the mode of delivery is.

Prevalence and Determinants of Exclusive Breastfeeding Practice among Mothers of Children Aged 6-24 Months in Hail, Saudi Arabia.

Background The WHO recommends that infants should be exclusively breastfed for the first six months of life to achieve optimal growth, development, and health. Nonadherence to exclusive breastfeeding (EBF) depends largely on the individual, sociocultural context, and institutional factors. The aim of this study is to estimate coverage and factors associated with adherence to EBF among mothers in the urban Hail region, Saudi Arabia. Methods A cross-sectional study was carried out during February–June 2019 among 450 mothers of children aged 6–24 months attending immunization and well-baby clinics in 6 primary healthcare centers in Hail city. A pretested structured questionnaire was used to interview the consented participants. Results The majority of mothers (72.9%) were aware of EBF; 24% reported initiation of breastfeeding within one hour after delivery; however, 71.1% did during the first 24 hours. The majority (76.8%) fed colostrum to their newborn; nevertheless, 50.1% had given a prelacteal feeding. Mothers who reported EBF practice were 50.7% (CI 45.9–55.4). The adjusted logistic regression analysis revealed that mother's awareness about EBF (aOR 3.03; 95% CI 1.78–5.18), antenatal care received at the governmental facility (aOR 2.63; 95% CI 1.28–5.41), breastfeeding a previous child (aOR 2.42; 95% CI 1.46–4.03), counseling received after delivery (aOR 2.47; 95% CI 1.34–4.53), and colostrum feeding given (aOR 4.24; 95% CI 2.31–7.77) were positively associated with EBF practice. On the other hand, mother's education (OR 0.39; 95% CI 0.15–0.99), higher family income (aOR 0.04; 95% CI 0.00–0.31), and practice prelacteal feeding (aOR 0.61; 95% CI 0.38–0.97) were negatively associated with EBF practice. Conclusion EBF rate in urban Hail is still far below WHO recommendations. Efforts to strengthen mothers' counseling/support during antenatal care and immediately after delivery are needed to promote EBF practice, especially in the private sector.

Impact of 2 Versus 1 Colostrum Meals on Failure of Transfer of Passive Immunity, Pre-Weaning Morbidity and Mortality, and Performance of Dairy Calves in a Large Dairy Herd.

Simple SummaryThis retrospective study of records from a dairy farm explored the impact of receiving a second feeding of colostrum 5–6 h after an initial meal of 3 L of quality-tested colostrum on calf health and productivity until the first lactation. Calves that received a second feeding were less likely to develop failure of transfer of passive immunity or morbidity pre-weaning than calves only receiving one feeding. Similarly, calves receiving a second feeding showed a greater growth rate until weaning and tended to produce more milk in the first lactation. However, receiving a second feeding was not associated with differences in pre-weaning mortality or reproductive efficiency. Our results show that feeding calves a second meal of colostrum within the first few hours of life has a positive impact on calf health and might have positive effects into the first lactation.Failure of transfer of passive immunity (FTPI) due to inadequate ingestion of colostral immunoglobulins by calves is associated with increased mortality and morbidity risks. Feeding calves a sufficient amount of quality-tested colostrum within the first hours of life is essential for successfully transferring passive immunity. Many farms have implemented a second meal of colostrum to maximize the opportunities for passive immunity transfer. However, excellent passive immunity can be achieved with a single feeding of sufficient quality-tested colostrum. Moreover, there is currently no evidence demonstrating the impact of a second colostrum feeding within 24 h of life in calves receiving adequate volumes of quality-tested colostrum in an initial feeding. Hence, the objective of this retrospective cohort study was to compare the risks of FPTI, pre-weaning morbidity and mortality, and growth and performance between dairy calves that received one or two feedings of colostrum. For this, the health and production records of a large dairy herd were analyzed. At this farm, newborn calves receive 3 L of quality-tested colostrum soon after birth, followed by another 2 L 5–6 h later. However, at times of shortages of colostrum, calves only receive the initial 3 L meal. The records of 2064 male and 2272 female calves were analyzed, where 4156 and 180 calves received 2 and 1 colostrum meals, respectively. Data from both sexes were included in the analysis of the risks of FTPI, morbidity, and mortality; however, only data from heifer calves were utilized for growth and performance analysis. Survival analysis, and logistic and linear regression models were used to investigate the impact of receiving two feedings of colostrum on calf FTPI status, morbidity, mortality, reproductive indices, pre-weaning average daily gain (ADG), and first lactation 305-d Mature Equivalent milk production (305ME). Calves that received two feedings of colostrum had lower odds of FTPI, a lower probability of being treated for respiratory disease, diarrhea, or any disease, and a greater pre-weaning ADG. However, there was no association between the number of colostrum feedings and pre-weaning mortality, and the probabilities of first insemination and first calving, although heifers receiving two colostrum feedings tended to receive fewer inseminations and to have a greater first lactation 305ME. Collectively, our results suggest that feeding calves a second feeding of colostrum 5–6 h after the initial feeding soon after birth could be an effective strategy to decrease FTPI and morbidity and optimize ADG in dairy calves pre-weaning.

English

The current study was planned to evaluate the transplacental transmission potentials of Anaplasma marginale among naturally infected adult dairy cattle in district Jhang, Punjab, Pakistan. A dairy farm was selected after a district level survey having highest number of blood smear, cELISA and PCR positives of A. marginale infected pregnant crossbred cows (Holstein Friesian × Cholistani). Blood samples were collected from dams (within 4 months of their pregnancies and at the time of parturitions) as well as from the new-born calves (before colostrum feeding within 3 h of the birth). Eighty pregnant cows were screened through the cELISA and PCR. Out of these, 54 were found positive for anaplasmosis. Further follow up revealed that 38 cows remained positive at the time of parturition. Finally, based on the criteria, 32 calves without colostrum feeding qualified for the vertical transmission trial. The results of present study revealed that 28 (9/32) and 13% (4/32) of the neonatal calves acquired intrauterine infection confirmed by cELISA and PCR, respectively. Overall occurrence of the transplacental transmission was 31%. Nevertheless, we concluded that transplacental transmission occurs and this route of transmission, can lead to a significant number of neonatal deaths. Hence, the transplacental route of disease transmission should also be considered for devising the prevention and control strategies regarding anaplasmosis in the dairy cattle. © 2021 Friends Science Publishers

Isolation and Molecular Characterization of Enterotoxigenic Escherichia coli (ETEC) Strains From Industrial Dairy Farms of Hamedan, Iran

Background: Enterotoxigenic Escherichia coli (ETEC) is considered as one of the most common causes of infectious diarrhea in calves, infecting animals during the first week of age. The secretory diarrhea is attributed to the virulence factors of ETEC strains mainly including heat stable toxin (STa), as well as F5 (K99) and F41 fimbriae. Objectives: The present study was undertaken to investigate ETEC infection in neonatal calves of industrial dairy farms of Hamedan, Iran. Additionally, it was undertaken to investigate the genotypic screening of virulence genes in enterotoxigenic E. coli isolated from from dairy farms calves of Hamedan county. Materials and Methods: A total of 120 rectal swab samples were collected from healthy and diarrheic calves at one week of age belonging to eight farms. Conventional bacteriological methods, multiplex PCR, and antibiotic susceptibility test of the ETEC isolates were performed. Results: Nine E. coli isolates were found to be ETEC strains, carrying STa enterotoxin along with F5 and/or F41 fimbriae as the indicators of ETEC cells. Additionally, antibiotic susceptibility test of the ETEC isolates revealed that all of them were sensitive to trimethoprim-sulfamethoxazole, ciprofloxacin, and enrofloxacin, whereas complete resistance was observed against amoxicillin-clavulanic acid (100%) and polymyxin B (100%). The present study, conducted for the first time in Hamedan, indicated a prevalence of 7.5% for Enterotoxigenic Escherichia coli in the examined animals. Conclusion: Regarding economic losses of the infection in claves as well as the zoonotic nature of ETEC cells, it is recommended that measures should be taken, such as immunization of pregnant cows prior to the delivery, feeding of adequate colostrum to newborn calves at the right time, and adherence to hygiene practices on the farms to prevent and/or reduce the incidence of diarrhea cases caused by infection with these bacteria.

Assessment of gaps of knowledge and practices of frontline community workers in Chandragiri Mandal, Chittoor district, Andhra Pradesh: maternal and child health services

Background: The undernutrition status among the vulnerable groups in rural areas still persists, despite provision of Anganwadi services in villages. Assessment knowledge and practices about maternal and child health services of frontline workers, will identify knowledge and implementation gaps. Objective was to assess the knowledge, attitude and practices of community health workers of maternal and child health services for identifying gaps for designing and implementing intervention.Methods: In this community based prospective, pre and post intervention-based study, Knowledge, Attitude and Practices (KAP) of the community health workers, in 16 villages and 13 Anganwadi centres of Chandragiri Mandal, Chittoor district, Andhra Pradesh about maternal and child health services were collected. The basic infrastructure of the anganwadis and health centres of Auxiliary nurse midwifes were assessed using a prevalidated survey instrument. Descriptive analysis was used.Results: The knowledge of antenatal and postnatal care was least among Anganwadi workers. Awareness regarding need of colostrum feeding was high in AWW compared to ASHA. ANM and AWW had lesser knowledge about duration of the exclusive breast feeding to the infant. Only 57.1% of ANM had sufficient knowledge about protein energy malnutrition consequences. AWW, ASHA did not have knowledge of vitamin A deficiency signs and symptoms although providing massive dose of vitamin A to children. ASHAs did not have awareness about anaemia and the knowledge was below 50% in ANMs.Conclusions: The study emphasizes the need for proper training with interactive sessions to these frontline workers, which will enhance their knowledge and skills for provision of quality services.

Accuracy of direct and indirect methods for assessing bovine colostrum quality using a latent class model fit within a Bayesian framework

Feeding high-quality colostrum is essential for calf health and future productivity. Therefore, accurate assessment of colostrum quality is a key component of dairy farm management plans. Direct and indirect methods are available for assessment of colostrum quality; however, the indirect methods are rapid, inexpensive, and can be performed under field settings. A hierarchical latent class model fit within a Bayesian framework was used to estimate the sensitivity (Se) and specificity (Sp) of the radial immunodiffusion (RID) assay, transmission infrared (TIR) spectroscopy, and digital Brix refractometer for the assessment of low-quality bovine colostrum in Atlantic Canada dairy herds. The secondary objective of the study was to describe the distribution of herd prevalence of low-quality colostrum. Colostrum quality of 591 samples from 42 commercial Holstein dairy herds in 4 Atlantic Canada provinces was assessed using RID, TIR spectroscopy, and digital Brix refractometer. The accuracy of all tests at different Brix value thresholds was estimated using Bayesian latent class models to obtain posterior estimates [medians and 95% Bayesian credibility intervals (95% BCI)] for each parameter. Using a threshold of <23% for digital Brix refractometer and <50 g/L for RID and TIR spectroscopy, median (95% BCI) Se estimates were 73.2 (68.4-77.7), 86.2 (80.6-91.0), and 91.9% (89.0-94.2), respectively. Median (95% BCI) Sp estimates were 85.2% (81.0-88.9) for digital Brix refractometer, 99.4% (97.0-100) for RID, and 90.7% (87.8-93.2) for TIR spectroscopy. Median (95% BCI) within-herd low-quality colostrum prevalence was estimated at 32.5% (27.9-37.4). In conclusion, using digital Brix refractometer at a Brix threshold of <23% could reduce feeding of low-quality colostrum to calves and improve colostrum and calf management practices in Atlantic Canada dairy herds. The TIR spectroscopy showed high Se in detection of low-quality colostrum. However, the RID assay, which is used as the reference test in several studies, showed limited Se for detection of low-quality colostrum.

Heat treatment of bovine colostrum: effects on colostrum metabolome and serum metabolome of calves

Bovine colostrum is important for neonates' health due to its nutritive and non-nutritive components. Heat treatment of colostrum is a well-established management tool, but it may influence colostrum components and affect the health status of calves. In our previous studies, we had shown that colostrum proteome and serum proteome of calves were altered by heat treatment to different degrees. Our objectives in this study were to investigate the effects of heat treatment on colostrum metabolome and the effect of feeding heat-treated colostrum on the serum metabolome of newborn calves. Further, the changes in serum metabolome from before to after colostrum feeding were characterized. Newborn Holstein female calves (n = 10) were randomized within pairs and fed heat-treated (n = 5; 60 °C, 60 min) or raw (n = 5) colostrum at 8.5% of birth BW by esophageal feeder within 1 h of birth. After a single colostrum feeding, calves were not fed until after the 8 h time point. Blood samples were taken immediately prior to feeding (0 h) and 8 h after feeding. The colostrum and serum metabolome were first analyzed using reverse-phase chromatography and tandem MS, and serum metabolome was then further analyzed using hydrophilic interaction chromatography and tandem MS. In colostrum metabolome, 458 features were identified and 328 were annotated and a trend of separation between raw and heat-treated colostrum could be observed through multivariate analysis. In serum metabolome, 3 360 features were identified and 1 439 were annotated, but no trend of separation was observed between the two groups of calves fed raw colostrum vs. heat-treated colostrum. The serum metabolome presented substantial differences comparing before (0 h) and after colostrum feeding (8 h); in particular, a tripeptide, β-homovaline-β-homoalanine-β-homoleucine, and 1-(2-acetamido-2-deoxy-α-d-glucopyranosyl)-1D-myo-inositol had higher concentrations after colostrum feeding than before, along with other metabolites that were not fully annotated. Based on a relatively small sample size, our findings point to the effect of heat treatment on the change of colostrum metabolome, but not on the change of serum metabolome of calves fed raw colostrum vs. heat-treated colostrum. Further studies using larger sample size and complementary analytical techniques are warranted to further explore potential heat treatment-induced alterations in colostrum metabolome.

Practical rules and tools of colostrum management

Colostrum management, from administration of dry cow vaccines to harvesting, feeding, and monitoring its success, is a significant opportunity for direct veterinary involvement and oversight on all dairy herds. It is well known there is a critical need for colostrum to provide calories and a source of immunoglobulins for transfer of immunity from dam to her naïve offspring. The purpose of this article is to review practical steps for helping dairy clients achieve these new guidelines, from the dry period to colostrum harvest, feeding, and monitoring colostrum programs.

A calf-level study on colostrum management practices associated with adequate transfer of passive immunity in Québec dairy herds

The objective of this study was to identify the calf-level colostrum management practices associated with an adequate transfer of passive immunity (TPI; defined as serum Brix refractance ≥8.4% in the first week of life) in small-sized herds. A total of 818 calves from 61 commercial Holstein dairy farms were included in this observational cross-sectional study. For each calf, sex, colostrum delivery method, colostrum volume fed at first meal, and time to first feeding (delay between birth and first colostrum meal) were noted. Blood and colostrum samples were collected to estimate the serum and colostrum quality using Brix refractometry. To quantify the level of bacterial contamination in colostrum samples, total bacteria count and total coliform count (TCC) were measured using the Petrifilm (3M, St. Paul, MN) culture system. In this study, 68% of calves had an adequate TPI (≥8.4%). For data distribution, the 25th, 50th, and 75th percentiles were 1.3, 2.8, and 3.3 L for the colostrum volume fed at the first meal; 20.9, 23.5, and 26.5% Brix; and 1.1, 3.1, and 6.5 h for the time to first feeding of colostrum, respectively. The odds of adequate TPI were 2.6 times higher in calves receiving ≥2.5 L colostrum at their first meal, 2.9 times higher in calves receiving colostrum with ≥24.5% Brix, and 1.6 times higher in calves receiving colostrum within 3 h after birth, than in calves not meeting these criteria. In the present study, median bacterial contamination distribution (interquartile range) in the first colostrum meal was 14,000 cfu/mL (3,000-83,000 cfu/mL) for total bacteria count, and 0 cfu/mL (0-1,000 cfu/mL) for TCC. Total bacteria count and TCC were not associated with the odds of adequate TPI in the final model. Overall, these results suggest that specific calf-level colostrum management practices are associated with adequate TPI in small- to medium-sized dairy herds.