Abstract -Thermal comfort is a state of mind that expresses satisfaction with the thermal environment. There are two main categories in the effort to obtain a comfort index; empirical and analytical. Empirical is based on social surveys, while analysis is based on the physics principle of heat flow.Bandung city is one of the historical cities in Indonesia which has many historical heritage buidings. One of them which is commonly known as colonial buildings in Bandung is Lautze 2 Mosque, located at Tamblong Street in the city center of Bandung. This research was conducted to analyze the thermal comfort in the Lautze 2 Mosque Bandung. The analytical method used in this study is a quantitative research method by measuring the main factors namely air temperature, air humidity and air velocity. The main factors become obstacles to get thermal comfort data at the Lautze 2 Mosque Bandung is the location of buildings located in areas with tropical climate conditions with high air temperatures, high air humidity, and low air velocitys.The research conclude that thermal comfort in the Lautze 2 Mosque Bandung building can be categorized as uncomfortable, it can be seen from the measurement results of related factors, namely the temperature and humidity that exceeds the comfort limit and the low air velocity in the building becomes an obstacle to obtain thermal comfort. Keywords: colonial buildings, mosque, thermal comfort, tropical climate.