This study was carried out in order to determine some morphological characteristics of 240 tomato genotypes at S2 level and to demonstrate the usability of these materials in breeding programs. Accordingly, the average fruit weight was between 553.11 gr and 74.83 gr. The mean pericarp thickness, carpel number, and soluble dry matter content of the genotypes were 7.38 mm, 3.6 pieces and 4.04% brix, respectively. Based on these measurements and observations, tomato genotypes were also investigated using the Cluster and Principal Component Analysis (PCA) methods. According to the PCA results, the first component explained 24.1% of the total variance, and the number of fruit carpels, average fruit weight, and fruit width were the most highly explained parameters. The second and third main components constituted 13.06% and 11.16% of the total. Based on the results of fruit characterization studies, genotypes 233, 447, 126, 22, 391, 118, 71, 100, 340, 102 were determined as superior. Overall, A high degree of morphological variation was detected among the characterized tomato genotypes.