Clinical Value Of Combined Detection Research Articles

Clinical value of combined detection of serum APE1-Aabs and CEACAM-1 in the diagnosis of colorectal cancer.

To analyze the clinical value of combined detection of serum APE1-Aabs and CEACAM-1 in pathological diagnosis of colorectal cancer. From December 2014 to July 2016, 60 patients with colorectal cancer and 50 healthy subjects were enrolled in this study. The levels of APE1-AAbs, CEACAM-1 and CEA in the serum were measured, and the clinical value of each index in the diagnosis of colorectal cancer was analyzed. The level of serum APE1-AAbs in colorectal cancer group was significantly higher than that in healthy control group (p < 0.05). The levels of serum CEACAM-1 and CEA in colorectal cancer patients were significantly higher than those in healthy control group (p < 0.05). The sensitivity and accuracy of APE1-AAbs and CEACAM-1 in the diagnosis of colorectal cancer were significantly higher than separate detection of APE1-AAbs, CEACAM-1 or CEA. Combined detection of serum APE1-AAbs and CEACAM-1 has the advantages of high sensitivity and good accuracy in the diagnosis of colorectal cancer, and it is worthy to be popularized in clinical practice.

Clinical value of combined detection of four common tumor markers in benign and malignant hepatobiliary system diseases

Clinical value of combined detection of four common tumor markers in benign and malignant hepatobiliary system diseases

Value of BCL-2 and PD-L1 Combined Detection in Predicting the Prognosis of Primary Acute Leukemia

To evaluate the clinical value of combined detection of BCL-2 and PD-L1 in predicting the prognosis of newly diagnosed patients with acute leukemia (AL). A total of 100 cases of AL in our hospital from Jan. 2013 to Sep. 2016 were enrolled in the study. The mRNA expression of BCL-2 and PD-L1 in peripheral blood of patients was detected by RT-PCR, and the prognosis of the patients was followed up. According to the follow-up results, these patients were divided into complete remission group and no remission group, the statistical analysis of different indexes predicting the prognosis was performed. Compared with the healthy control group, the positive rates of BCL-2 and PD-L1 in the bone marrow specimens of AL patients significantly increased (P<0.01). The BCL-2 predicting the prognosis of patients showed that the area under the curve (AUC) was 0.725(P=0.006), the diagnostic threshold was 1.550, the sensitivity(Sen) and specificity(Spe) were 72% and 84%, respectively. The single PD-L1 detection for predicting the prognosis of patients showed that AUC was 0.740 (P=0.004), diagnostic threshold was 12.500, Sen and Spe were 66.7% and 73.1%, respectively. The diagnostic index of combined detection was 77.90, which were higher than that of series detection 54.75, and higher than that of the independent diagnostic index. Detection of BCL-2 combined with PD-L1 can be used to evaluate the prognosis of patients with newly diagnosed acute leukemia, which can improve the specificity and sensitivity of the detection, and has higher clinical diagnostic value than single actection.

The clinical value of combined detection of plasma Lp-PLA2 and D-dimer in patients with hypertension complicated with stroke

Objective To investigate the predictive value of plasma lipoprotein phospholipase A2(Lp-PLA2) and D-dimer in patients with hypertension complicated with stroke. Methods 70 hypertensive patients with stroke were selected as A group, 72 patients with simple hypertension were selected as B group, and 71 healthy subjects were selected as C group.The light transmitting method was used to detect serum Lp-PLA2, and immunoturbidimetry was used to detect plasma D-dimer.The clinical value of the two joint detection and single detection in prediction of hypertension complicated with stroke was analyzed. Results The plasma Lp-PLA2 and D-dimer levels of A group were (471.88±181.49)ng/mL, (982.54±373.28)μg/mL, which were higher than those of B group[(195.17±34.74)ng/mL, (263.57±29.70)μg/mL] and C group[(122.94±46.25)ng/mL, (96.44±8.49)μg/mL](F=189.7, 171.1, all P=0.000). The plasma Lp-PLA2 level of A group was positively correlated with D-dimer(r=0.692, P 252g/mL forecast hypertension stroke risk sensitivity was 83.3% and specificity was 98.6%, the plasma Lp-PLA2>176.36ng/mL forecast hypertension stroke risk sensitivity was 92.9% and specificity was 83.1%, and the combined prediction of hypertension stroke risk sensitivity was 87.6%, specificity was 93.5%. Conclusion The plasma levels of Lp-PLA2 and D-dimer are significantly increased in hypertensive patients with stroke, and the combined detection of the two indicators can improve the prediction of stroke risk in patients with hypertension. Key words: Hypertension; Apoplexy; Plasma lipoprotein phospholipase A2; D-dimer

Clinical value of combined detection of serum CD4+ T cells and CD8+ T cells for advanced gastric cancer

Objective To explore the clinical value of combined detection of serum CD4+ T cells and CD8+ T cells for advanced gastric cancer (AGC). Methods Serum levels of CD4+ T cells and CD8+ T cells were measured by flow cytometry and compared in 20 patients with AGC (experimental group) and 20 healthy adults (control group). Also, serum levels of CD4+ T cells and CD8+ T cells were measured by flow cytometry and compared before and after surgical treatment in the patients of experimental group. Results The serum levels of CD4+ T cells were decreased and CD8+ T cells were increased in patients with AGC. However, the serum levels of CD4+ T cells were increased and CD8+ T cells were decreased after surgical treatment (P<0.05). Conclusions The serum levels of CD4+ T cells and CD8+ T cell help to assess the immune status of patients with AGC, which contributes to the illness monitoring for AGC. Key words: Stomach neoplasms/IM; CD4-positive T-lymphocytes/IM; CD8-positive T-lymphocytes/IM

Clinical significance of combined liver function and high-sensitivity C-reactive protein measurement in children with hand-foot-mouth disease.

Hand-foot-mouth disease (HFMD) is a common pediatric disease responsible for the development of rashes or herpes on the hand, foot, and mouth. Severe complications of HFMD include myocarditis, pulmonary edema, aseptic meningoencephalitis, and even death. Therefore, early diagnosis of HFMD is of particular importance. In this study, we determined the clinical value of the combined detection of liver function and high-sensitivity C-reactive protein (hs-CRP) expression in children with HFMD. Three hundred children with HFMD were recruited to this study between July 2013 and July 2015 and divided into the mild and severe HFMD groups (N = 150 per group). The liver function [aspartate aminotransferase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) levels] and hs-CRP expression were evaluated using standardized tests, and the clinical value of combined detection of these indices (in parallel and serially) was determined. Patients in the severe HFMD group showed significantly higher levels of ALT, AST, ALP, and hs-CRP compared to those in the mild HFMD group (P < 0.05). The hs-CRP and liver function tests had low specificity and sensitivity, respectively. However, parallel combined detection improved the sensitivity and negative predicted value of these indices, whereas serial combined detection improved the specificity and positive predicted value. In conclusion, hs-CRP and liver function play a major role in the diagnosis of HFMD (and identifying its severity), and serial combined detection of these indices enhances the positive predicted value, and could be employed to diagnose severe HFMD at an earlier stage.

Clinical value of combined detection of serum CA724, CEA and TNF-α for advanced gastric cancer

Objective To explore the clinical value of combined detection of serum carbohydrate antigen 724 (CA724), carcinoembryonic antigen (CEA), and tumor necrosis factor-α (TNF-α) for the diagnosis, assessment of curative effect, and illness monitoring for advanced gastric cancer (AGC). Methods Serum levels of CA724, CEA and TNF-α were measured by the methods of chemiluminescence and compared in 20 patients with AGC (Group A ), 20 patients with benign gastric disease (Group B), and 20 healthy adults (Group C). Also, serum levels of CA724, CEA and TNF-α in peripheral blood as well as in portal vein were measured and compared before and after surgical treatment in patients of group A. Results The serum levels of CA724, CEA and TNF-α in patients of group A were significantly higher than groups B and C (P<0.05). In addition, the serum levels of CA724, CEA and TNF-α in patients of group A were decreased after surgical treatment and increased slightly after one month (P<0.05). Conclusions The serum levels of CA724, CEA and TNF-α in most patients of AGC are significantly elevated and the combined detection of serum CA724, CEA and TNF-α contributes to the diagnosis, assessment of curative effect, and illness monitoring for AGC. Key words: Stomach neoplasms/DI/TH/ME; Antigens, tumor-associated, carbohydrate/ME; Carcinoembryonic antigen/ME; Tumor necrosis factor-alpha/ME

The clinical value of combined detection of GGT, AFU, DCP and AFP in diagnosis of hepatocarcinoma

Objective To detect the serum levels ofγ-glutamyltransferase(GGT),α-L-fucosidase(AFU),des-gamma-carbory prothrombin(DCP),and alpha fetoprotein(AFP) hepato carcinoma patients,and explore its clinical predictive value in the diagnosis of hepatocarcinoma. Methods GGT and AFU was measured by velocity method,radioimmunoassay was used in DCP measurement,and ELISA assay was adopted to measure AFP.The levels of serum GGT, AFU,DCP and AFP were measured in 47 patients with primary hepatic carcinoma,35 patients with secondary liver cancer,and 56 normal people.All results were analyzed by statistic method. Results The positive rates of serum GGT,AFU, DCP and AFPin primary hepatic carcinoma and secondary liver cancer were 89.4%,83.0%,74.5%,76.6% and 82.9%, 48.6%,68.6%,62.9%,respectively,which were higher than those of normal people(χ2GGT= 11.05,χ2AFU= 7.35,χ2DCP= 8. 34,χ2AFP= 14.58,all P<0.01).Combined detection of the four index,the positive rates were 95.7% and 88.6%,which was higher than that of normal people(χ2= 10.13,P<0.05). Conclusion Combined detection the levels of serum GGT,AFU, DCP and AFP can help the early diagnosis of hepatic carcinoma. Key words: Liver Neoplasms; Early Diagnosis; Transaminases; α-L-fucosidase; Prothrombin; alpha-Fetoproteins

Study on combined detection of serum β-HCG, estradiol, progesterone and antiphospholipid antibodies in predicting the outcome of threatened abortion in early pregnancy

Objective To explore the clinical value of combined detection of serum beta human chorionic gonadotropin(β- hCG), estradiol(E2), progesterone(P)and antiphospholipid antibodies(ACA)in predicting the outcomes of early threatened abortion. Methods 80 pregnant women were chosen from six to eight weeks singleton pregnancies, 50 cases of threatened abortion and 30 cases of normal pregnancy. The serum levels ofβ- HCG, estradiol, progesterone and ACA- IgG,ACA- Ig M and ACA- IgA were detected. The threatened abortion patients were given intramuscular progesterone 20 mg/day/time for 1 week, then, the pregnancy outcomes were analyzed and the serum levels ofβ- HCG,estradiol and progesterone were explored. Results The serum β-HCG, estradiol and progesterone of the threatened abortion group[(5 140.77±2 365.47)IU/L,(680.28±306.06)pg/mL,(15.59±3.65)pg/L]were lower than those of the normal pregnant women[(14 520.4±11 016.24)IU/L,(1 142.81±670.57)pg/mL,(27.6±4.78)pg/L](t= 3.224,P= 0.003;t = 2.43,P= 0.022;t= 7.72,P= 0.001). According to pregnancy outcome,patients with threatened miscarriages were divided into the two groups, including 45 patients with continuing pregnancies and 5 patients with inevitable miscarriages.The serum levels ofβ- HCG, estradiol and progesterone in ongoing pregnancies group[(10 829.42±6 926.28)IU/L,(832.9±397.91)pg/m L,(25.62±4.51)pg/L]were higher than those in inevitable miscarriages[(6 310.14±2 593.19)IU/L,(487.14±275.47)pg/m L,(13.6±4.84)pg/L](P< 0.05); The serum levels of ACA- IgG,ACA- Ig M and ACA- Ig A in 80 cases tested,only 2 cases who appeared in the threatened abortion group were detected with the positive of serum ACA- IgG,accounted for 4% (2/50)(χ2= 68.05,P= 0.000), occurred spontaneous abortion in the end. Conclusion Combined detection of serum ACA,β-HCG, estradiol and progesterone may be helpful for prognosis of threatened abortion. However, the clinical predictive value of ACA needs further study in the early threatened abortion. Key words: Threatened abortion; beta human chorionic gonadotropin(β-hCG); Estradiol; Progester-one; Antiphospholipid antibodies; Pregnancy outcome

Clinical value of combined detection of serum decoy receptor 3 and CA19-9 in diagnosis of pancreatic cancer

目的：探讨血清中诱骗受体3（decoy receptor3,DcR 3）和CA19-9联合检测对胰腺癌诊断的临床价值.方法：收集90例胰腺癌、20例胰腺良性肿瘤患者及20例健康对照者血清,应用酶联免疫吸附试验（ELISA）方法测定Dc R3水平,电化学发光免疫分析法测定血清CA19-9水平,采用受试者工作特征（receiver operating characteristic,ROC）曲线评估他们对胰腺癌诊断和可切除性判断的临床价值.结果：胰腺癌患者血清Dc R3和CA19-9水平分别为37.75 pg/m L和202.29 k U/L,明显高于胰腺良性肿瘤组和健康对照组,差异均有统计学意义（P〈0.01）.Dc R3和CA19-9诊断胰腺癌的曲线下面积（area under ROC curves,AUC）分别为0.81和0.89,两者联合检测的AUC为0.95.Dc R3和C A19-9对胰腺癌科切除性判断的AUC分别为0.68和0.59,两者联合检测的AUC为0.73.结论：Dc R3和CA19-9在胰腺癌患者血清中升高,两者联合检测有助于提高胰腺癌诊断价值和可切除性的判断.

Clinical significance of combined detection of D-Dimer, coagulation four indices and hs-CPR level for coronary heart disease

Objective To explore the clinical value of combined detection of D-dimer,coagulation four indices and hs-CRP in diagnosis of coronary heart disease (CHD).Methods D-Dimer,coagulation four indices and hsCPR level were detected in 67 patients with CHD[22 stable angina(SA) pectoris patients,20 unstable angina (UA) pectoris patients and 25 acute myocardial infarction (AMI) patients] and 20 healthy people(normol control group).According to the result of coronary arteriongraphy,the patients with CHD were divided into single vessel disease group (n =21),double vessel disease group (n =23) and three vessel disease group (n =23).The levels of plasma D-D,fibrinogen(FIB) and hs-CRP were detected and compared.Results Compared with UA group,SA group,control group respectively,D-D,fibrinogen and hs-CRP level of AMI group had statistical significance (P ＜ 0.01) ;Compared with SA group,control group,D-D,fibrinogen and hs-CRP level of UA group had statistical significance (P ＜ 0.05) ;Compared with control group,hs-CRP level of SA group had statistical significance (P ＜ 0.01).The comparision of PT,APTT and TT level between each two groups,there was no statistical difference (P ＞ 0.05).The levels of plasma DD and FBG in single vessel disease group,double vessel disease group and three vessel disease group were significantly higher than those in NC group(P ＜ 0.05).The levels of plasma DD and FBG in three vessel disease group were significantly higher than those in single vessel disease group and double vessel disease group(P ＜ 0.05).The levels of plasma DD and FBG in double vessel disease group were significantly higher than those in single vessel disease group (P ＜ 0.05).The comparision of PT,APTT and TT level between each two groups,there was no statistical difference (P ＞ 0.05).Conclusion The level of D-D,FIB and hs-CRP have close relationship with the severe degree of CHD and can help clinical doctor to predict the type of CHD. Key words: Coronary Arteriosclerosis ; D-dimer ; Hs-CRP

Clinical value of combined detection of serum human epididymal secretory protein E4 and CA(125) in the diagnosis of endometrial carcinoma

To explore the clinical value of combined detection of serum human epididymal secretory protein E4 (HE4) and CA(125) in the diagnosis of endometrial carcinoma. From Jan 2010 to Apr 2011, the serum specimens were collected from 124 cases of endometrial carcinoma, 97 cases of benign disease of uterus and 109 cases of healthy women. HE4 levels in the serum were detected by ELISA, and CA(125) levels in the serum were detected by the electro-chemiluminescent immunoassay. Those results were shown with median level. Accuracy of the diagnosis was evaluated by the area under the receiver operating characteristic curve (ROC-AUC). The median levels of HE4 and CA(125) were 78.09 pmol/L and 33.43 kU/L in serum of endometrial carcinoma group. The median levels of HE4 and CA(125) were 46.37 pmol/L and 18.26 kU/L in serum of benign disease of uterus group. The median levels of HE4 and CA(125) were 31.75 pmol/L and 12.64 kU/L in serum of healthy women group. The HE4 and CA(125) levels in serum of endometrial carcinoma group were significantly higher than those of benign disease of uterus group or healthy women group (all P < 0.05). Compared with that benign disease of uterus group, the ROC-AUC of HE4 and CA(125) in endometrial carcinoma group were 0.913 and 0.801, respectively. When the specificity was 95.0%, the sensitivities of HE4, CA(125), and combined detection of HE4 and CA(125) in endometrial carcinoma group were 41.1%, 22.6% and 46.0%, respectively. The positive rates of HE4 and CA(125) were 31% (27/86) and 12% (10/86) in stage I-II of endometrial carcinoma, while the positive rates were 63% (24/38) and 47% (18/38) in stage III-IV of endometrial carcinoma, in which there were significant difference between patients in stage III-IV and stage I-II (P < 0.01). The combined detection of serum HE4 and CA(125) is helpful to the diagnosis of endometrial carcinoma. The sensitivity, specificity and early diagnosis of HE4 are better than that of CA(125). The positive rates of HE4 and CA(125) in endometrial carcinoma are related to the clinical staging.

Clinical Value of serum CA125 ,CA72-4 and TSGF in ovarian cancer

Objective To investigate the clinical value of combined detection of the serum levels of CA125,CA72-4 and tumor specific growth factor(TSGF)in the diagnosis of ovary cancer and the curative effect.Methods Serum levels of CA125,CA72-4 and TSGF in 68 patients with ovary cancer,53 patients with benign ovary rumor and 50 normal controls were measured by chemiluminescent immunoassay(CLIA)and chemical colorimetry.Results and clinical data were statisticaly analyzed.Results The levels of CA125,CA72-4 and TSGF in ovary cancer group were higher than that in the benign ovarian tumor group and healthy controls(all P ＜0.01).When use three tumor markers united detection,sensitivity,specificity and accuracy were 91.2％,83.0％ and 87.4％ respectively.The sensitivity and accuracy was higher than any single detection.The specificity with single CA125 testing was consistently.The levels of CA125,CA72-4 and TSGF in patients with ovary cancer were significantly different after surgical treatment for five days(all P ＜0.05).Conclusion The combined detection of the three markers may increase the positive rate in the early diagnosis of ovarian cancer,and help to differentiate the benign and malignant ovarian tumor,but also is valuable to observe the curative rate and postoperative monitor of the ovary cancer. Key words: Ovary neoplasms; Carbohydrate antigen; Tumor specific growth factor

Clinical value of combined detection of CA 1 9 - 9, CEA, AFP and Fer in diagnosis of digestive system neoplasm

Objective To explore the clinical value of combined detection of serum carbohydrate antigen 19-9(CA19-9) ,carcinoembryonic antigen( CEA), alpha-fetoprotein(AFP) and ferritin(Fer) in the diagnosis of digestive system neoplasm. Methods 183 cases of digestive system neoplasm and 40 patients with benign digestive diseases and 40 healthy contrll were selected. The serum tumor marker levels were detected by electrochemiluminescence assay. Results In digestive system neoplasm patients and patients with benign digestive disease, the level of the tumor markers were increased,while the detection rate of four joint detection test than a single tumor marker in digestive system neoplasm were significantly increased. Conclusion Combined detection of serum CA19-9, CEA, AFP, and Fer level, could significantly improve the sensitivity of diagnosis of digestive tract tumors. Key words: Tumor markers; Carbohydrate antigen 19-9 ; Carcinoembryonic antigen; Alpha-fetoprotein; Ferritin

The value of MG7‐Ag and COX‐2 for predicting malignancy in gastric precancerous lesions

Here we aimed to first investigate the clinical value of combined detection of MG7-Ag and COX-2 (cyclo-oxygenase 2) in prediction of advances in gastric precancerous lesions. Immunohistochemical analysis was used to examine the expression of MG7-Ag and COX-2 in 396 cases of patients with gastric precancerous lesions, including 66 cases of atrophic gastritis, 106 cases of intestinal metaplasia, 174 cases of low-moderate-grade dysplasia and 50 cases of high-grade dysplasia. The relation of MG7-Ag and COX-2 staining with various clinicopathological features was analysed by follow-up study. The positive rates of MG7-Ag and COX-2 were increased gradually from atrophic gastritis (21.2%, 28.8%), intestinal metaplasia (36.8%, 44.3%), low-moderate-grade dysplasia (51.4%, 58.6%) to high-grade dysplasia (72%, 80%). Double positive staining of MG7-Ag and COX-2 in gastric precancerous lesions had an increased risk of precancerous progression over 22 times, compared with negative ones. However, the expression of MG7-Ag and COX-2 was not significantly correlated with age and gender of patients. MG7-Ag and COX-2 might play an important role in the process of carcinogenesis and progression of gastric cancer. Combined detection of MG7-Ag and COX-2 was of value of predicting early gastric cancer from precancerous lesions.

Clinical value of combined detection of tumor markers for nasopharyngeal carcinoma and squamous cell carcinoma in head and neck

Objective To study the clinical value of serum SCC,CYFRA21-1,NSE and CEA for patients with nasopharyngeal carcinoma (NPC) and squamous cell carcinoma of head and neck (NHSCC). Methods From Aug 2004 to Sep 2007, the serum levels of SCC, CYFRA21-1, NSE and CEA were detected in 79 NPC and 51 HNSCC before and after radiotherapy. The clinical related factors were analyzed. Results Before treatment, the positive rates of serum SCC, CYFRA21-1, NSE and CEA for patients with NPC were 10.53 %, 46.75 %, 17.5 % and 2.04 %, respectively. The sensitivity of combined detection was 78.95 %. The positive rates for patients with HNSCC were 22 %, 19.75 %, 11.11% and 0, respectively. The sensitivity of combined detection was 36.17 %. The level of SCC was related to staging in HNSCC group. CYFRA21-1 was related to the rote of treatment response in NPC group. Conclusion The sensitivity of combined detection of tumor markers were significantly increased. CYFRA21-1 associated with NPC and SCC with HNSCC were noted in this study. Key words: Head and neck neoplasms; Nasopharyngeal neoplasms; Radiotherapy, computer-assisted; Tumor markers, biological

Clinical value of combined detection of hepatitis C virus antibody and core antigen

Objective To discuss the clinical application value of combined detection of hepatitis C virus antibody and core antigen(HCV-cAg).Methods Serum sample were combined tested for HCV antibody and HCV-cAg by enzyme-linked immnosorbent assy(ELISA)kit,then the results were compared with those detected by fluores-cent quantitation PCR for hepatitis C virus ribonucleic acid(HCV-RNA).Results Among the 157 patients,149 were positive for HCV-Ab,99 positive for HCV-cAg and 115 for HCV-RNA.Respectively:5 patients who were negative for HCV antibody and positive for HCV-cAg were confirmed to be HCV infection by HCV-RNA.154 patients were positive for HCV antibody or HCV-cAg,the other 3 were excluded from HCV infection.Conclusion Combined detection of HCV antibody and HCV-cAS can improve the detection rate of HCV infection,avoid omission effectively,and is suitable for promotion in common medical institutions. Key words: HCV core antigen; HCV antibody; HCV—RNA

Clinical value of combined detection of AFP, CA125, CA199 and CEA in the diagnosis of primary hepatic carcinoma

Objective To study the clinical value of combined detection of the serum levels of AFP, CA125, CA199 and CEA in the diagnosis of primary hepatic carcinoma (PHC). Methods Serum levels of AFP, CA125, CA199 and CEA in 68 patients with PHC, 50 patients with benign liver disease and 58 normal controls were measured by Electrochemiluminescence immuno-assay (ECLIA). Results The levels of AFP, CA125, CA199 and CEA in PHC group were significantly higher than those in benign liver disease and normal controls. Sensitivities of AFP, CA125, CA199 and CEA were 76.4 %, 35.3 %, 44.1%, 29.4 %, respectively. Sensitivity of combined detection of four kinds tumor markets was 94.1%. Conclusion Combined detection of four kinds of serum tumor markers is useful to increase the detective positive rate in the diagnosis of PHC. Key words: Liver neoplasms; Radioimmunoassay; Serologic tests

Clinical value of combined detection of tumor necrosis factor-α, carbohydrate antigen 19-9 and carcinoembryonic antigen in gastric fluid of elderly patients with gastric cancer

Clinical value of combined detection of tumor necrosis factor-α, carbohydrate antigen 19-9 and carcinoembryonic antigen in gastric fluid of elderly patients with gastric cancer