Cherry Tomato Research Articles

Application of active and environment-friendly poly (3-hydroxybutyrate)/grapeseed oil/MgO nanocomposite packaging for prolonging the shelf-life of cherry tomatoes (Solanum lycopersicum L. var.cerasiforme)

Active packaging is one of the emerging technologies for maintaining the safety and quality of fresh produce. This work assessed the effectiveness of an active and eco-friendly poly (3-hydroxybutyrate) (PHB) based film loaded with 5 wt% grapeseed oil (GS) and 0.7 wt% MgO nanoparticles (MgO NPs) prepared via sonication-assisted solution casting route for storage and handling of the cherry tomatoes. The shelf-life study of cherry tomatoes in active (PHB/5GS/0.7MgO) packaging was carried out for 23 days in ambient room conditions, and results are compared with the storage of cherry tomatoes without packaging (control), in low-density polyethylene (LDPE) packaging, and in PHB packaging. Various parameters, viz. Visual appearance, weight loss, firmness, total soluble solids (TSS), titratable acidity (TA), ripening index, pH, color, and organoleptic factors, were evaluated every 3 days for cherry tomatoes in various storage groups. The results from the shelf-life study clearly showed that the cherry tomatoes stored in PHB/5GS/0.7MgO packaging experienced better preservation of quality than those in other storage groups, with no signs of surface wrinkling, shrinkage, microbial growth, or juice leakage till day 21 of their storage. This was further evidenced by minimal weight loss (4.45 ± 0.06% on day 21), minimal change in titratable acidity (0.74 ± 0.01% on day 0–0.67 ± 0.01% on day 21), and better retention of firmness (19.89 ± 0.12 N on day 0–15.77 ± 0.37 N on day 21), pH, color, and organoleptic attributes for the cherry tomatoes stored in this packaging group. Furthermore, it was found that the migration of MgO NPs from the nanocomposite packaging to cherry tomatoes was negligible. These findings suggest that the PHB/5GS/0.7MgO nanocomposite film is a promising choice for the storage of cherry tomatoes.

Antioxidant Profile, Amino Acids Composition, and Physicochemical Characteristics of Cherry Tomatoes Are Associated with Their Color.

This study was conducted to characterize different colored lines of cherry tomatoes and derive information regarding their metabolite accumulation. Different colored cherry tomato cultivars, namely 'Jocheong', 'BN Satnolang', 'Gold Chance', 'Black Q', and 'Snacktom', were assessed for their firmness, taste characteristics, and nutritional metabolites at the commercial ripening stage. The cultivars demonstrated firmness to withstand impacts during harvesting and postharvest operations. The significant variations in the Brix to acid ratio (BAR) and the contents of phenylalanine, glutamic acid, and aspartic acid highlight the distinct taste characteristics among the cultivars, and the nutritional metabolites are associated with the color of the cultivars. The cultivar choices would be the black-colored 'Black Q' for chlorophylls, β-carotene, total flavonoids, and anthocyanins; the red-colored 'Snacktom' for lycopene; the orange-colored 'Gold Chance' for total phenolics; and the green-colored 'Jocheong' for chlorophylls, vitamin C, GABA, glutamic acid, essential amino acids, and total free amino acids. The antioxidant capacity varied among the cultivars, with 'Gold Chance' consistently exhibiting the highest activity across the four assays, followed by 'Snacktom'. This study emphasizes the importance of screening cultivars to support breeding programs for improving the nutritional content and encourages the inclusion of a diverse mix of different colored cherry tomatoes in packaging to obtain the cumulative or synergistic effects of secondary metabolites.

Nisin/cellulose nanofiber/protein bio-composite antibacterial coating for postharvest preservation of fruits

The urgent need to prolong the shelf life of fruits and vegetables in a cost-effective and environmentally friendly manner has become paramount in addressing socio-economic challenges and minimizing food waste. In this study, a bio-composite coating was developed for fruit and vegetable preservation, utilizing egg protein as the bio-based film-forming component, egg yolk as the hydrophobic element, cellulose nanofibers for reinforcement, and Nisin as an antimicrobial agent. The preservation efficacy of this coating was examined using bananas and cherry tomatoes, and its mechanism was analyzed. The findings revealed that the coating successfully formed a thin layer on the surface of the fruits, ranging in thickness from nanometers to micrometers. This innovative coating prolonged the shelf life of the fruits by over 6 days under 20 °C, preserving their appearance, hardness, and nutrient content during storage. By inhibiting gas exchange and warding off microbial infestation, the coating effectively slowed the ripening and decay processes. Additionally, its edibility and washability alleviated food safety concerns without compromising the fruit's natural taste. This research offers an innovative solution to the global food waste problem, serving as an environmentally responsible and highly scalable method for maintaining freshness.

Flexible Multicavity SERS Substrate Based on Ag Nanoparticle-Decorated Aluminum Hydrous Oxide Nanoflake Array for Highly Sensitive In Situ Detection.

Flexible surface-enhanced Raman scattering (SERS) substrates are very promising to meet the needs for real-time and on-field detection in practical applications. However, high-performance flexible SERS substrates often suffer from complexity and high cost in fabrication, limiting their widespread applications. Herein, we developed a facile method to fabricate a flexible multicavity SERS substrate composed of a silver nanoparticle (AgNP)-decorated aluminum hydrous oxide nanoflake array (NFA) grown on a polydimethylsiloxane (PDMS) membrane. Strong plasmon couplings promoted by multiple nanocavities afford high-density hotspots within such a flexible AgNPs@NFA/PDMS film, boosting high SERS sensitivity with an enhancement factor (EF) of ∼1.54 × 109, and a limit of detection (LOD) of ∼7.4 × 10-13 M for rhodamine 6G (R6G) molecules. Furthermore, benefiting from the high sensitivity, high mechanical stability, and transparency of this substrate, in situ SERS detections of trace thiram and crystal violet (CV) molecules on the surface of cherry tomatoes and fish have been realized, with LODs much lower than the maximum allowable limit in food, demonstrating the great potential of such a flexible substrate in food safety monitoring. More importantly, the preparation processes are very simple and environmentally friendly, and the techniques involved are completely compatible with well-established silicon device technologies. Therefore, large-area fabrication with low cost can be readily realized, enabling the extensive applications of SERS sensors in daily life.

Modulation of cherry tomato performances in response to molybdenum biofortification and arbuscular mycorrhizal fungi in a soilless system

Molybdenum (Mo) is a crucial microelement for both, humans and plants. The use of agronomic biofortification techniques can be an alternative method to enhance Mo content in vegetables. Concomitantly, arbuscular mycorrhizal fungi (AMF) application is a valuable strategy to enhance plant performances and overcome plant abiotic distresses such as microelement overdose. The aim of this research was to estimate the direct and/or indirect effects of Mo supply at four doses [0.0, 0.5 (standard dose), 2.0 or 4.0 μmol L−1], alone or combined with AMF inoculation, on plant performances. In particular, plant height and first flower truss emission, productive features (total yield, marketable yield and average marketable fruit weight) and fruit qualitative characteristics (fruit dry matter, soluble solids content, titratable acidity, ascorbic acid, lycopene, polyphenol, nitrogen, copper, iron and molybdenum) of an established cherry tomato genotype cultivated in soilless conditions were investigated. Moreover, proline and malondialdehyde concentrations, as well as Mo hazard quotient (HQ) in response to experimental treatments were determined. A split-plot randomized experimental block design with Mo dosages as plots and +AMF or -AMF as sub-plots was adopted. Data revealed that AMF inoculation enhanced marketable yield (+50.0 %), as well as some qualitative traits, such as fruit soluble solids content (SSC) (+9.9 %), ascorbic acid (+7.3 %), polyphenols (+2.3 %), and lycopene (+2.5 %). Molybdenum application significantly increased SSC, polyphenols, fruit Mo concentration (+29.0 % and +100.0 % in plants biofortified with 2.0 and 4.0 μmol Mo L−1 compared to those fertigated with the standard dose, respectively) and proline, whereas it decreased N (−25.0 % and −41.6 % in plants biofortified with 2.0 and 4.0 μmol Mo L−1 compared to those fertigated with the standard dose, respectively). Interestingly, the application of AMF mitigated the detrimental effect of high Mo dosages (2.0 or 4.0 μmol L−1). A pronounced advance in terms of plant height 45 DAT, fruit lycopene concentration and fruit Fe, Cu and Mo concentrations was observed when AMF treatment and Mo dosages (2.0 or 4.0 μmol Mo L−1) were combined. Plants inoculated or not with AMF showed an improvement in the hazard quotient (HQ) in reaction to Mo application. However, the HQ - for a consumption of 200 g day−1 of biofortified cherry tomato - remained within the safety level for human consumption. This study suggests that Mo-implementation (at 2.0 or 4.0 μmol L−1) combined with AMF inoculation could represent a viable cultivation protocol to enhance yield, produce premium quality tomato fruits and, concomitantly, improve Mo dose in human diet. In the light of our findings, further studies on the interaction between AMF and microelements in other vegetable crops are recommended.

Fruit Quality Analysis and Flavor Comprehensive Evaluation of Cherry Tomatoes of Different Colors.

Cherry tomatoes are popular vegetables worldwide owing to their variety of colors and nutrients. However, an integrated evaluation of color and flavor has rarely been reported. This study examined the differences among red, brown, yellow, and green cherry tomatoes grown in the Jiuquan area. A comprehensive analysis of the flavor quality of these tomatoes, including sensory evaluation, electronic nose analysis, nutritional and flavor quality measurements, targeted metabolomics, and chemometrics, was conducted. Red tomatoes had the highest lycopene content, and green tomatoes had the highest soluble protein and vitamin C content. In cherry tomatoes, K is the most abundant macro element and Fe and Zn are the most abundant trace elements. Brown cherry tomatoes had significantly higher K, P, Mg, Cu and Fe contents than other colored tomatoes, and red tomatoes had significantly higher Zn content than other cherry tomatoes (218.8-724.3%). Yellow cherry tomatoes had the highest soluble sugar content, followed by red, brown and green tomatoes. A total of 20 amino acids of tomatoes were simultaneously determined by LC-MS. Yellow cherry tomatoes have the highest content of essential amino acids, aromatic amino acids and sweetness amino acids. Red tomatoes have the highest levels of non-essential and sourness amino acid contents. An analysis of 30 flavor indicators revealed that yellow tomatoes had the best flavor, followed by red, brown, and green tomatoes. Our work lays the foundation for future research on color and flavor formation in cherry tomatoes.

Multifunctional and edible egg white/amylose-tannin bilayer film for perishable fruit preservation

The substantial waste of perishable foods during transportation significantly contributes to greenhouse gas emissions, intensifying the climate crisis. To mitigate the rapid spoilage of fruits, an eco-friendly bilayer film was developed using natural egg white (EW), amylose (Am), and tannic acid (TA). The EW/Am-TA bilayer film features a primary layer of amphiphilic EW, ensuring a uniform coating on hydrophobic fruit surfaces, and a secondary layer composed of Am and TA, imparting notable tensile strength (5.3 ± 0.5 MPa) and elongation at break (28.5 ± 4.1 %). This bilayer film effectively shields fruits from UV-B and UV-C radiation (~0 % transmittance at 280 and 330 nm) and exhibits antioxidant and antibacterial properties due to the presence of TA. Fruits such as bananas, avocados, and cherry tomatoes, when dip-coated with the optimized EW/Am-TA bilayer, maintained their freshness, color, weight, and texture for up to seven days, demonstrating the effectiveness of this bilayer coating in food preservation. The natural materials in the coated film are edible and can be safely removed with tap water at room temperature in <10 s, posing no food safety risks. Thus, the proposed bilayer coating presents a significant solution to the global problem of food waste.

A selective harvesting robot for cherry tomatoes: Design, development, field evaluation analysis

AbstractWith the aging population and increasing labor costs, traditional manual harvesting methods have become less economically efficient. Consequently, research into fully automated harvesting using selective harvesting robots for cherry tomatoes has become a hot topic. However, most of the current research is focused on individual harvesting of large tomatoes, and there is less research on the development of complete systems for harvesting cherry tomatoes in clusters. The purpose of this study is to develop a harvesting robot system capable of picking tomato clusters by cutting their fruit‐bearing pedicels and to evaluate the robot prototype in real greenhouse environments. First, to enhance the grasping stability, a novel end‐effector was designed. This end‐effector utilizes a cam mechanism to achieve asynchronous actions of cutting and grasping with only one power source. Subsequently, a visual perception system was developed to locate the cutting points of the pedicels. This system is divided into two parts: rough positioning of the fruits in the far‐range view and accurate positioning of the cutting points of the pedicels in the close‐range view. Furthermore, it possesses the capability to adaptively infer the approaching pose of the end‐effector based on point cloud features extracted from fruit‐bearing pedicels and stems. Finally, a prototype of the tomato‐harvesting robot was assembled for field trials. The test results demonstrate that in tomato clusters with unobstructed pedicels, the localization success rates for the cutting points were 88.5% and 83.7% in the two greenhouses, respectively, while the harvesting success rates reached 57.7% and 55.4%, respectively. The average cycle time to harvest a tomato cluster was 24 s. The experimental results prove the potential for commercial application of the developed tomato‐harvesting robot and through the analysis of failure cases, discuss directions for future work.

Rapid in situ formation of κ-carrageenan-carboxymethyl chitosan-kaolin clay hydrogel films enriched with arbutin for enhanced preservation of cherry tomatoes

Food waste resulting from perishable fruits and vegetables, coupled with the utilization of non-renewable petroleum-based packaging materials, presents pressing challenges demanding resolution. This study addresses these critical issues through the innovative development of a biodegradable functional plastic wrap. Specifically, the proposed solution involves the creation of a κ-carrageenan/carboxymethyl chitosan/arbutin/kaolin clay composite film. This film, capable of rapid in-situ formation on the surfaces of perishable fruits, adeptly conforms to their distinct shapes. The incorporation of kaolin clay in the composite film plays a pivotal role in mitigating water vapor and oxygen permeability, concurrently bolstering water resistance. Accordingly, tensile strength of the composite film experiences a remarkable enhancement, escalating from 20.60 MPa to 34.71 MPa with the incorporation of kaolin clay. The composite film proves its efficacy by preserving cherry tomatoes for an extended period of 9 days at 28 °C through the deliberate delay of fruit ripening, respiration, dehydration and microbial invasion. Crucially, the economic viability of the raw materials utilized in the film, coupled with the expeditious and straightforward preparation method, underscores the practicality of this innovative approach. This study thus introduces an easy and sustainable method for preserving perishable fruits, offering a cost-effective and efficient alternative to petroleum-based packaging materials.

Inhibition Mechanism of Water-Soluble Chitosan-Curdlan Composite Coating on the Postharvest Pathogens of Serratia marcescens and Pseudomonas syringae in Cherry Tomatoes.

Cherry tomatoes, a very popular fruit, are highly susceptible to microbial infestation, which cause significant economic losses. In order to preserve cherry tomatoes better, we treat them with a Chitosan (CTS) and Curdlan (CUR) composite coating. The lowest inhibitory concentration of CTS/CUR composite coating on Serratia marcescens and Pseudomonas syringae, the growth curves, and the changes of the cell lysis rate were determined to explore the inhibitory mechanism of CTS/CUR composite coating on Serratia marcescens and Pseudomonas syringae and the microscopic morphology of Serratia marcescens and Pseudomonas syringae was observed using scanning electron microscopy at the same time. The results showed that the CTS/CUR composite coating could effectively inhibit the growth of Serratia marcescens and Pseudomonas, and the inhibitory effect reflected the concentration-dependent characteristics. The electron microscopy results indicated that the inhibition of Serratia marcescens and Pseudomonas syringae by the CTS/CUR composite coating might originate from its disruptive effect on the cell wall and cell membrane of the bacterium.

Comparison of biodegradable and non-biodegradable packaging for extending the shelf life of round and cherry tomatoes

Comparison of biodegradable and non-biodegradable packaging for extending the shelf life of round and cherry tomatoes

Pigmentos fotossintéticos, crescimento, e produção de tomate cereja sob estresse salino e peróxido de hidrogênio

ABSTRACT Excess of salts in water and/or soil stands out as one of the stresses that compromise the development of crops, including cherry tomato, requiring the use of strategies that reduce salt stress effects, such as foliar application of hydrogen peroxide. Thus, the objective of this study was to evaluate the effect of foliar application of H2O2 on the contents of photosynthetic pigments, growth, and production of cherry tomato under irrigation water salinity. The design used was randomized blocks, in a 5 × 5 factorial scheme, with five values of electrical conductivity of water - ECw (0.3, 1.0, 1.7, 2.4, and 3.1 dS m-1) and five concentrations of hydrogen peroxide - H2O2 (0, 15, 30, 45, and 60 μM). Application of hydrogen peroxide at concentration of 60 μM attenuated the deleterious effects of salt stress on the growth and number of leaves of cherry tomato up to ECw of 3.1 dS m-1. Hydrogen peroxide concentration of 45 μM increased root dry mass, synthesis of chlorophyll b and total chlorophyll under electrical conductivity of water of 0.3 dS m-1 and synthesis of chlorophyll a up to ECw of 0.8 dS m-1. Foliar application of 30 μM reduced the effects of salt stress on leaf, shoot and total dry mass accumulation up to ECw of 3.1 dS m-1. Electrical conductivity of water from 1.41 dS m-1 reduced the production of cherry tomato.

GGE analysis and stability of traits in tomato cultivars grown under organic farming conditions: A two-year study

The increasing conversion of agricultural land to organic farming requires the development of specifically adapted cultivars. So far, in tomato there is lack of research for selection of germplasm suitable for sustainable agroecosystems. In this study, we investigated the genotypic and environmental factors affecting the variation of plant, fruits, and root traits in 39 tomato genotypes grown under organic farming conditions. Four independent experiments were conducted in Italy and Spain across two consecutive seasons in 2019 and 2020. For all traits, the factorial linear regression model to estimate the main effects of genotype (G), location (L), year of cultivation (Y) and their interactions, revealed highly significant (P < 0.001) variations, with the G factor being largely predominant for most traits. The implementation of the “which-won-where”, “mean performance versus stability” and “discriminative vs representativeness” patterns in the GGE (Genotype plus Genotype by Environment interaction) analysis, allowed the identification of superior cultivars with high stability across the testing environments. Genomic characterization with 30 890 high quality SNPs from ddRADseq genotyping analysis, revealed that a specific cluster of cherry tomato accessions were low performing in terms of yield and fruit weight, on the contrary, showed a high content of soluble solids, which in agreement with GGE analysis. Results of this study provide a framework for the potential use of this locally adapted tomato germplasm to address the needs of more sustainable agriculture.

Exploring Co-labeling Strategies in Local Food Markets: Insights from a Choice Experiment on Southern Buyers’ Preferences for Cherry Tomatoes

Labeling strategies are often discussed in the context of local food purchase. Substantial research has been undertaken to discern buyers’ preferences for different labeling strategies associated with a production practice or a geographic location. Some studies have also emphasized the substitution or complementarity effects that may occur across these different labels. Using a large choice experiment with 1820 respondents across six US southern states, this research evaluates buyers’ preferences for co-labeling strategies, focusing on the association of a production practice and certifications (USDA Organic and Certified Naturally Grown) alongside six different production locations, ranging from local to imported sources. We focus on pint baskets of cherry tomatoes, chosen due to their popularity among purchasers of fresh produce. Based on the results provided by a Bayesian Mixed Logit model, we derived the respondent-specific posterior distribution of the partworths associated with each production location and regressed each of those against demographic indicators. Our findings highlight that most buyers substitute between USDA Organic and Certified Naturally Grown (CNG), and a minority consistently opt for the same production practice option. In addition, we underscore that price, or an indication of origin predominantly guides nearly half of buyers’ choices. We find that the premium for CNG is slightly superior to the organic one. Last, older respondents and respondents with a higher degree of education value produce grown within their state over neighboring states and more distant origins.

Effect of shellac coating on the properties of egg white protein (EWP) films for cherry tomato packaging

Effect of shellac coating on the properties of egg white protein (EWP) films for cherry tomato packaging

The Use of Ultraviolet Irradiation to Improve the Efficacy of Acids That Are Generally Recognized as Safe for Disinfecting Fresh Produce in the Ready-to-Eat Stage.

Fresh-cut produce is usually produced under standardized disinfection processes, which are unavailable at the ready-to-eat stage. Currently, chemical sanitizers are used for washing, but their disinfection efficacy is limited. In this study, UV-C (1.03 kJ/m2) was combined with organic acids that are generally recognized as safe (GRAS), including citric, malic, acetic, and lactic acids (LAs), to wash lettuce and cherry tomatoes that are contaminated with Escherichia coli O157:H7 and Salmonella Typhimurium. The results showed that LA was the most effective treatment among the single treatments, with a pathogen reduction and cross-contamination incidence of 2.0-2.3 log CFU/g and 28-35%, respectively. After combining with UV-C, the disinfection efficacy and cross-contamination prevention capacity of the four GRAS acids significantly improved. Among the combination treatments, the highest pathogen reduction (2.5-2.7 log CFU/g) and the lowest cross-contamination incidence (11-15%) were achieved by LA-UV. The analyses of ascorbic acid, chlorophyll, lycopene, antioxidant capacity, and ΔE indicated that neither the single nor combination treatments negatively affected the quality properties. These results provide a potential hurdle technology for fresh produce safety improvement at the ready-to-eat stage.

Mechanically robust carboxymethyl cellulose/graphene oxide composite cross-linked by polyetherimide for fruits packaging and preservation system

Modulating the interactions between biopolymer matrix and nanofillers highly determined the mechanical performances of composite packaging materials. Herein, we innovatively proposed a sort of eco-friendly and mechanically robust carboxymethyl cellulose/graphene oxide/tannic acid/polyetherimide (CMC/GO/TA/PEI, CGTP) composite by employing PEI as cross-linker and TA as proton donor. The amidation reaction between -NH2 and -COOH chemically connected the CMC/GO, CMC/CMC and GO/GO and the physical interaction (e.g. hydrogen bonds and molecular entanglements) was beneficial to form dense structures. The chemical/physical bonds among polymers and nanofillers contributed to dissipate the external energy. The toughness was effectively reinforced from 1.68 MJ/m3 for CGTP0 to 4.63 MJ/m3 for CGTP1.0. Furthermore, the CGTP1.0 composite film also delivered improved gas (moisture and oxygen) barriers, UV protection and antimicrobial features. Originating from these merits, the shelf life of fresh fruits (e.g. strawberries, blueberries and cherry tomatoes) was prolonged at least 5 days under ambient conditions when the packaging box was covered by the fabricated CGTP1.0 film. Our findings not only provided a facial strategy to reinforce the interactions between biopolymer matrix and nanofillers, but also boosted the development of eco-friendly packaging materials with robust structures in the area of food packaging.

The antimicrobial synergy of polymer based nanofiber mats reinforced with antioxidants intercalated layered double hydroxides as a potential active packaging material

Perishable food post-harvest loss is a major global concern, and research is currently concentrated on creating active packaging materials. This research is focused in multiple antioxidants intercalated Layered Double Hydroxides (LDH) that are combined in one matrix, and their overall effect that defines as synergism, which successfully preserves perishable food by releasing antioxidants slowly. For this purpose, a hybrid LDH material of ascorbic-LDH (AA-LDH), salicylic-LDH (SA-LDH), and citric-LDH (CA-LDH) was synthesized, characterized and incorporate into electrospun nanofiber mat to be used as a potential active packaging material. Antioxidants intercalated Mg/Al LDH was synthesized and successfully characterized by PXRD, FTIR, XPS, Raman, SEM, and EDS. The shifts in the LDHs’ peaks in PXRD indicated the successful incorporation of antioxidants into LDH. FTIR, Raman, and XPS data clearly indicated the establishment of metal-oxygen bonds by observing the characteristic peaks. Morphological features and the layered structure were clearly observed by SEM images. Antioxidants were slowly released from the LDHs, and it was evaluated for time intervals up to 24 h. The hybrid LDH material exhibited the highest antioxidant activity with an IC50 value of 132.5 μg ml−1, where 234.1, 354.5, and 402.2 μg ml−1 were reported for ascorbic-LDH, salicylic-LDH, and citric-LDH respectively. The hybrid LDH material incorporated electrospun mats showed the best antibacterial activity against the tested bacteria and clearly evidenced the synergistic activity of the combination of the nanohybrids. It has showed a minimal bacterial growth compared to the other control samples (∼2.41 log CFU/ml). The shelf life of cherry tomatoes was studied at different physiochemical parameters with and without hybrid LDH material incorporated electrospun mats. The fabricated mat showed an extended shelf life of 42 days for cherry tomatoes, whereas the control sample showed a shelf life of 17 days. It is concluded that hybrid LDH material exhibited synergistic performance and the best antioxidant activity when comparing with mono LDH materials.

Effect of Different Sources of Nitrogen and Bio Fertilizers on Growth, Yield and Quality of Cherry Tomato [Solanum lycopersicum L.) Var. Cerasiforme] in Polyhouse Conditions

The present investigation was carried out at Research Farm, Department of Horticulture, Naini Agricultural Institute, SHUATS, Naini, Prayagraj, Uttar Pradesh during the Kharif-2022 with a view to identify the effect of different sources of nitrogen and bio fertilizers on growth, yield and quality of cherry tomato” [Solanum lycopersicum L.) var. cerasiforme] in polyhouse conditions. The experiment was laid in Randomized block design (RBD) with 9 treatments and 3 replications with different combination in RDF and application of organic nutrition. Under this experiment, overall, 10 treatment were taken. From the above experimental finding it may be concluded that the treatment T9 (75% N through urea + 25% N through FYM + Azotobacter (4 kg ha-1) + PSB (4 Kg ha-1) was found to be best in the terms of growth parameters like highest plant height (283.35 cm) at 120 DAT, maximum number of branches per plant (72.33 branches) at 120 DAT. In terms of earliness, it was found to have minimum days to attain 50% flowering (55.93 DAT). In terms of yield T9 had highest number of fruits per cluster (5.57 fruits), and fruit yield per hectare (25.88 t ha-1).

Characterization and efficacy of Salmonella phage cocktail PHA46 in the control of Salmonella Newport and Typhimurium internalized into cherry tomatoes

Non-typhoid Salmonella enterica causes salmonellosis illness, and this bacterium can contaminate food throughout the production chain, including those that are consumed as raw products. Salmonella enterica can adhere to and internalize into fresh produce such as cherry tomatoes. It has been reported that lytic bacteriophages (phages) can be used as a biocontrol agent in the agricultural field, being an alternative for the control of Salmonella in red meat, fish, lettuce, and cabbage. The aim of this study was to characterize the two phages present in the PHA46 cocktail to determine their morphology, genome, host range, and resistance to different temperatures and pHs values; and later evaluate their lytic activity to reduce the adherence to and internalization of Salmonella enterica serovars Newport and Typhimurium into cherry tomatoes. In addition, in this work, we also explored the effect of the PHA46 cocktail on the virulence of S. Newport-45 and S. Typhimurium SL1344, recovered from the interior of cherry tomatoes, on the lifespan of the animal model Caenorhabditis elegans. The nematode C. elegans, recently has been used to test the virulence of Salmonella and it is easy to maintain and work with in the laboratory. The results revealed that the morphology obtained by Transmission Electron Microscopy of two phages from the PHA46 cocktail correspond to a myovirus, the analyses of their genomes sequences did not report virulence or antimicrobial resistance genes. The PHA46 sample is specific for 33 different serovars from different Salmonella strains and shows stability at 7 °C and pH 6. Also, the PHA46 cocktail was effective in reducing the adherence of S. Newport-45 and S. Typhimurium SL1344 to cherry tomatoes, at an average of 0.9 log10, respectively. Regarding internalized bacteria, the reduction was at an average of 1.2 log10, of the serovars mentioned above. The lifespan experiments in C. elegans showed by itself, that the PHA46 cocktail was harmless to the nematode, and the virulence from both Salmonella strains grown in vitro is diminished in the presence of the PHA46 cocktail. In conclusion, these results showed that the PHA46 cocktail could be a good candidate to be used as a biocontrol agent against Salmonella enterica.