Chemical Injury Research Articles

CLINICAL OUTCOMES AND EVALUATION OF CHEMICAL INJURIES OF THE EYE

Background: Ocular chemical injuries are a true ocular emergency and require immediate and intensive evaluation and treatment. The sequels of chemical injury may have signicant detrimental visual and psychological effects on the affected individual. Effective diagnosis and treatment within time following the initial injury often dictate the clinical course and can prevent tragic consequences. The aim of the present study is to study the clinical prole & visual outcome of ocular chemical injuries A Methodology: descriptive observational study was carried out including 100 eyes of 78 patients after meeting inclusion and exclusion criteria. Detailed history was taken and thorough ocular examination was carried out pertaining to the injury. Visual acuity was recorded by Snellen's chart. Clinical grading was done by Roper Hall Classication. Ocular investigations were done when required. Patients were managed medically and/ or surgically. Final visual outcome and slit lamp ndings were noted at Day 1,1 Week, 1 Month and 3 months for each affected eye. The Results: study included 77% (60) males and 23% (18) females. Mean age was 25 ± 10 yrs. Alkali injuries (68%) was more common than acid injuries (32%). Calcium carbonate (lime) 35% was the most common etiological agent. 56% cases had unilateral involvement. Maximum patients belonged to grade 1 (51%) followed by grade II (27%) and minimum to grade IV (7%). Final visual outcome was found to be dependent on initial grade and visual acuity. Presenting visual acuity and grade are important prognosti Conclusion: c factors. Higher grades of injuries (grade III and IV) have poorer outcomes than grade I and II. Final visual acuity is also dependent on grade of injury and presenting visual acuity.

Ocular Trauma During COVID-19 Pandemic: A Systematic Review and Meta-analysis.

This study aimed to summarize the latest literature on the trends and incidence of ocular trauma during the COVID-19 pandemic. Systematic review and meta-analysis. A systematic literature search was conducted to identify the relevant literature. The search period was between January 1, 2020, and September 20, 2021. The incidence of overall and various types of ocular trauma during the COVID-19 pandemic and the control period was analyzed. The data from different studies were pooled. The odds ratio (OR) and 95% confidence interval (CI) were calculated. A total of 32 articles were included. After pooling the data from all included studies, the incidence of total and pediatric ocular trauma during the COVID-19 pandemic was 67.7% and 54.3% of those in the control period, respectively. However, the proportion of ocular trauma in eye emergency visits increased during the pandemic (OR, 95% CI: 1.46, 1.04-2.06). The proportion of domestic ocular trauma increased (OR, 95% CI: 3.42, 1.01-11.62), while ocular trauma related to sports and outdoor activities and occupational ocular trauma decreased (OR, 95% CI: 0.64, 0.09-4.29 and 0.18, 0.10-0.33, respectively). It was also reported that chemical injury caused by alcohol-based sanitizers, photokeratitis caused by ultraviolet lamps, and mechanical eye injury caused by masks increased during the COVID-19 pandemic. There was a reduction in overall eye injuries and substantial differences in the spectrum of ocular trauma during the COVID-19 pandemic. Proper health education and supervision should be strengthened to prevent ocular injuries related to COVID-19 preventive interventions.

In Vitro Regeneration Capacity In Response To Chemical Injury Is Compromised In Aged Vs Young Muscle Cells

Healthy skeletal muscle can regenerate after ischemic, mechanical or toxin-induced injury. However, age-related changes in skeletal muscle cells and their niche may impair the regeneration potential. While strategies to regenerate muscle are being developed, in vitro models to investigate the regenerative potential after injury in aged versus young muscle cells are lacking. PURPOSE: This study aims to develop in vitro muscle models of injury and regeneration using cells from older and young donors. METHODS: Differentiated C2C12 murine and human myoblasts from old and young donors were injured after exposure to 12% barium chloride for 6 h. Regeneration was assessed by morphological analysis including myotube width, number of nuclei and fusion index. For that cells were stained with phalloidin and DAPI to label the cytoskeleton and nuclei of muscle cells, respectively. Images were acquired with the Leica fluorescence microscope and analysed using ImageJ. Timepoints were pre-injury (control), post-injury, end of proliferation and end of differentiation. RESULTS: The number of myonuclei post-injury remained unaltered compared to pre-injury among all cell lines (p > 0.05). Three days post-injury, nuclei number reached significance only in murine (p = 0.0006) and young (p = 0.03) muscle cells, whereas aged myotubes showed a significant increase in the total number of myonuclei at the end of the regeneration period (p = 0.01). Myotube diameter at the end of the regeneration recovered to pre-injury state in C2C12 and young but was significantly smaller in aged cells (p = 0.04). CONCLUSION: Delayed peak in proliferation and significantly smaller myotubes in aged compared to young and murine muscle cells at the end of the experiment could be attributed to declined proliferation rate of resident satellite cells that causes suspended myogenesis and regenerative response in older muscle cells. Barium chloride injury model is an easily reproducible in vitro approach that could be applied to study age and/or disease-related regenerative changes in different cell lines. KEYWORDS: regeneration, injury, skeletal muscle, ageing

A STUDY ON OCULAR CHEMICAL INJURIES WITH THEIR WISUAL OUTCOMES

Ocular chemical Injury comprises about 8-18% of overall ocular trauma. It is caused by various types of acids and alkalis.Alkalis penetrate deep and cause severe injury. Acid cause lesser severity of burns.It is more common in age group of 20-40 years.It is the true ocular emergency and requires immediate intervention. It can cause a greviousvisual impairment in younger economically productive age group. Hence, it is necessary to evaluate the damages caused by it with optimal intervention to restore vision. This study enables the analysis of current management of ocular chemical injury and its effect on the visual outcomes of the patients.

Development of potential COX inhibitor as anti-inflammatory agents from leaves of Lantana camara by in-vitro analysis, molecular docking and ADMET prediction

Lantana camara have several uses in medicinal field for the treatment of various diseases like ulcer, tetanus, asthma, rheumatism, malaria and cancer. Following these properties, it is expected that it may have good COX-1/2 inhibition property which is responsible for inflammation at the site of physical and chemical injury. The fractions were tested for COX-1/2 inhibition activity by in-vitro method. Further following obtained data reported phytochemicals of the plant were In silico screened for their COX-1/2 inhibition activity by molecular docking method. Among all the extracts the methanolic extract shows highest percentage inhibition against COX-2 (91.87% IC50 value 4.06 ± 0.42 μg/ml) and COX-1 (81.87%).The molecular docking study of pre-isolated phytochemicals from the leaf of plant also shows that compound 1, 6, 7, 8, 30 and 32 possess a great inhibition potential against COX-1 and COX-2 while ligand 30 and 1 shows selectivity for COX-2 over COX-1.

The role of Tenonplasty in the management of limbal and scleral ischemia due to acute ocular chemical burns.

Of the various manifestations of ocular chemical burns (OCBs), ischemia of the limbus and the peri-limbal sclera indicates poor prognosis and in severe cases threaten the integrity of the globe. Tenonplasty is a surgical procedure which involves advancing the Tenon's capsule over the ischemic areas to provide a vascular supply and to enable migration of the conjunctival epithelium. This review aims to provide an overview of the diagnosis of limbal ischemia and its management with Tenonplasty. A literature review was conducted using the keywords "Tenonplasty," "Tenon's capsule," "ocular chemical injury," "ocular thermal injury," "Tenon advancement," "scleral ischemia," and "limbal ischemia," and outcomes were studied from seven selected articles. In addition to clinical evaluation, in vivo imaging techniques such as anterior segment optical coherence tomography angiography can provide an objective method of measuring and monitoring the ischemia and re-perfusion of the peri-limbal vasculature. Tenonplasty can be performed in eyes with acute OCBs with scleral or limbal ischemia by dissecting the Tenon's layer from the orbit and securing it to the limbus. The indications, mechanism of action, peri-operative considerations, surgical technique, and post-operative care of Tenonplasty are discussed in detail. The average time for post-operative re-epithelization ranges from 1 to 6 months with the formation of a symblepharon being the most common complication. In conclusion, Tenonplasty is a globe-salvaging procedure in cases with severe limbal and scleral ischemia because of OCBs and has good anatomical outcomes priming the globe for subsequent re-constructive and vision-restoring surgeries.

903 Improving Documentation of Tourniquet Use in a Trauma Unit in Line with New BOAST Guidelines

Abstract Aim Local tissue damage is a significant potential consequence of intraoperative pneumatic tourniquet use, via pressure-related damage or chemical injury. The British Orthopaedic Association released a guideline relating to the safe intraoperative tourniquet use in October 2021, dictating standards for documentation in operative records. Initial audit suggested that only 15% of the required information was documented. With updated operative record paperwork and electronic templates, we aimed to improve adherence to updated BOAST guidance. Method Following analysis of 14 days' worth of trauma operation records, results of local documentation of tourniquet use were presented at a departmental audit meeting. With departmental permission, changes were made to the operation notes template, to include tickboxes relating to the use of tourniquets, skin condition pre/post-operatively, method of isolation, method of exsanguination, and the pressure of tourniquet and duration of use in line with new guidelines. Additionally, a new, time-efficient template was designed for those using electronic records. Results were re-audited over a 14-day period following dissemination of the relevant templates. Results Initial results suggested that only 15% of required data surrounding tourniquet use was being documented, with none recording information relating to skin condition pre/post-operatively, method of isolation or exsanguination. Following aforementioned interventions led to an improvement in documentation from 15% compliance to 64% compliance. Conclusions Further efforts are required in order to further improve documentation of tourniquet use, particularly when in electronic format, initial re-audit results are encouraging for improved adherence to new BOAST guidance in a local trauma unit.

Comparative evaluation of marginal accuracy of different provisional restorative materials fabricated by direct technique: An in vitro study

Objective: The most essential requirement of a provisional crown is an adequate marginal fit which is necessary for maintaining optimal periodontal health and protect the tooth from physical, chemical, thermal, and bacterial injuries. The purpose of this in vitro study was to evaluate and compare the vertical marginal accuracy of provisional crown materials using four different material systems (Autopolymerized PMMA powder-liquid system, light-activated UD single paste system, chemically activated Bis-GMA two paste auto mix system and dual cure polymerized resin two paste auto mix system) using a direct technique. Materials and Methods: Two customized stainless-steel dies, simulating prepared and unprepared tooth were used to fabricated a total no. of 48 provisional crowns. A vacuum-formed polypropylene sheet was used as a matrix. Twelve crowns, each of the four material systems used in the study (n = 12 × 4) were fabricated using the direct technique. The vertical marginal accuracy was analyzed by using stereomicroscope 20X magnification at four different points.

LOCAL ADVERSE EFFECTS CAUSED BY THE USE OF TOPICAL IMIQUIMOD ON ORAL MUCOSA: CASE REPORT

Imiquimod (INN) can cause chemical injury in the oral mucosa, leading to local irritation, including erythema, ulceration, and pain. We report a case of a 34-year-old male patient presenting a symptomatic lesion on the lower and upper lip mucosa. The patient reported that the lesion appeared after applying INN 5% cream prescribed by a dermatologist as treatment for a white, plaque-shaped, asymptomatic lower lip lesion. Clinically, the lesion presented redness and extensive ulceration and erosion area, which extended throughout the upper and lower labial mucosa. Therefore, the diagnostic hypothesis of chemical injury induced by topical INN was considered. Request for INN suspension and prescription of oral prednisone and topical clobetasol propionate were made. After 3 weeks, total lesion resolution was observed. Thus, we highlight that topical INN can cause local adverse effects, and an effective diagnosis is of utmost importance to promote adequate treatment.

Combining fecal microbiome and metabolomics to reveal the disturbance of gut microbiota in liver injury and the therapeutic mechanism of shaoyao gancao decoction.

Chemical liver injury is closely related to gut microbiota and its metabolites. In this study, we combined 16S rRNA gene sequencing, 1H NMR-based fecal metabolomics and GC-MS to evaluate the changes in gut microbiota, fecal metabolites and Short-chain fatty acids (SCFAs) in CCl4-induced liver injury in Sprague-Dawley rats, and the therapeutic effect of Shaoyao Gancao Decoction (SGD). The results showed that CCl4-induced liver injury overexpressed CYP2E1, enhanced oxidative stress, decreased antioxidant enzymes (SOD, GSH), increased peroxidative products MDA and inflammatory responses (IL-6, TNF-α), which were ameliorated by SGD treatment. H&E staining showed that SGD could alleviate liver tissue lesions, which was confirmed by the recovered liver index, ALT and AST. Correlation network analysis indicated that liver injury led to a decrease in microbiota correlation, while SGD helped restore it. In addition, fecal metabolomic confirmed the PICRUSt results that liver injury caused disturbances in amino acid metabolism, which were modulated by SGD. Spearman’s analysis showed that liver injury disrupted ammonia transport, urea cycle, intestinal barrier and energy metabolism. Moreover, the levels of SCFAs were also decreased, and the abundance of Lachnoclostridium, Blautia, Lachnospiraceae_NK4A136_group, UCG-005 and Turicibacter associated with SCFAs were altered. However, all this can be alleviated by SGD. More importantly, pseudo germ-free rats demonstrated that the absence of gut microbiota aggravated liver injury and affected the efficacy of SGD. Taken together, we speculate that the gut microbiota has a protective role in the pathogenesis of liver injury, and has a positive significance for the efficacy of SGD. Moreover, SGD can treat liver injury by modulating gut microbiota and its metabolites and SCFAs. This provides useful evidence for the study of the pathogenesis of liver injury and the clinical application of SGD.

Chemical Injury Due to Pain Balm: Case Series

Ocular chemical injuries accounts for 11.5% to 22.1% of ocular injuries. Ocular chemical burns are an ophthalmic emergency and requires immediate treatment. We report a series of three cases of chemical injury secondary to accidental exposure to red balm used as local application for headache. In our cases menthol, cajuput oil and capsaicin might have contributed to chemical injury and nerve damage. Nerve damage might have lead to delayed epithelial healing. All three patients improved completely within 1 to 3 week.

Community-level awareness of proper immediate steps regarding ocular chemical injury in Asir Region

Background: Although the eyes represent 0.1% of the total human body, such organs are exposed to multiple injuries, some of which possibly lead to permanent loss of vision. Chemical injuries occurring on the eyes is deemed to be a major ophthalmic emergency, requiring immediate clinical assessment and initiation of treatment. Concerning the awareness of the Saudi population regarding the risks of chemical-based ophthalmic injuries and the permanent repercussions brought about by such incidents, together with the proper steps and actions that should be taken in such cases, this has unfortunately still a degree of paucity within the state. In addition, it is of great importance to recognize areas of shortcomings by health care workers, in order to provide accurate and non-complex medical facts regarding chemical-based ophthalmic injuries Aims: This study focused on evaluating the general population’s awareness of the immediate responses that are required, following ophthalmic chemical injury incidents within the Asir province of Saudi Arabia. Methodology: This study contained a questionnaire that was randomly distributed across all segments of population in the Asir province of the Kingdom of Saudi Arabia (KSA), through various social media, entitled ‘The eyes are arguably the most important sensory organ of the human body’. This investigation lasted a total time span of six months (May – October 2021) and its design was classified as a descriptive cross-sectional survey of the local Asir community. Results: In relation to overall population awareness regarding chemical eye injury within the Asir region, KSA, among this cohort, 288 (48%) had good awareness level regarding chemical eye injury and its management, while 312 (52%) had poor awareness. Through our research we found out that individuals in the Asir population require greater awareness regarding the immediate steps of management in cases of ocular chemical eye injuries. Furthermore, there is a need to establish that the only solution required to irrigate the eye is water, with a mechanism of eye washing from the middle part of the face to the tip of the eye. Conclusions: These results should be evaluated by the Ministry of Health and the appropriate actions should be made, such as health awareness campaigns, regarding ocular chemical injuries and immediate corrective actions, in order to improve knowledge and to create a healthier society. Key words: ocular chemical injury, community awareness, Abha

839 Early biomarker identification for immune sensitization and prevention with oral vitamin D3

Reaction to chemical irritants is a common cause of dermatology consultation and a challenge for topical medication utility. Cholecalciferol (D3) mitigates inflammation from experimental sunburn in humans and chemical injury in mice. To investigate the clinical utility of D3 for chemical injury in humans, we conducted a double-blinded, placebo-controlled trial. 28 healthy subjects had 3x8mm2 of arm skin exposed to 0.0016% topical nitrogen mustard (NM) under occlusion. 2 weeks later, subjects were randomized to receive 200,000 IU oral D3 or placebo after repeat NM exposure on the other arm. By proteomic analysis of skin biopsies, placebo subjects have 22% more differentially expressed inflammatory proteins (DEP) than D3 subjects after 3 days and 650% after 6 weeks. PCA of RNAseq separates samples by exposure yet shows 2 intervention-independent subclusters in NM exposure 2. One shows a normal skin profile (NS) and the other shows immune memory cell enrichment, suggesting immune sensitization (S). S subjects show more inflammation than NS by skin erythema (p<0.01), edema (p=0.05), and histopathologic score (p<1e-4). S and NS D3 subjects express fewer DEPs than placebo at 3 days (S:41%;NS:82%) and 6 weeks (S:86%;NS:100%). Subgroup analysis of exposure 1 identifies early S-associated biomarkers. After exposure 2, NS subjects express no S markers if given D3 vs 75% if given placebo. Results identify biomarkers of sensitization that may have predictive clinical utility and demonstrate that oral D3 mitigates chemically-induced inflammation for up to 6-weeks. Thus, D3 may have preventative properties against chemical sensitization and serve as a low-risk treatment and prevention in those undergoing repetitive chemical exposure.

577 Synthetic melanin nanoparticles improve wound healing

Excessive release of reactive oxygen species (ROS) during acute skin injury has detrimental effects on wound healing. Since natural melanin is an efficient scavenger of radical species, we hypothesized that a topical treatment consisting of synthetic melanin nanoparticles (SMNP) would benefit the healing process. We utilized the mouse chemical skin injury model to test our hypothesis, using nitrogen mustard (NM) for injury induction. Topical treatment with SMNP resulted in significantly improved wound healing as evidenced by the rate of wound area reduction (p=0.006 at day 3, n=17), decreased edema (p=0.003 at day 2, n=9-10), and time of eschar detachment compared to the vehicle group (p=0.005, n=9-10).

Precision of limbal ischemia evaluation in ocular chemical injuries with anterior segment optical coherence tomography angiography.

To evaluate the limbal ischemia objectively in ocular surface chemical injuries by using anterior segment optical coherence tomography angiography (AS-OCTA). In this cross-sectional study, acute ocular surface chemical injury patients with less than 1week injury history were enrolled. Demographic data of the patients were noted, and detailed ophthalmological examination with Dua classification was performed. AS photographs and AS-OCTA images were obtained and used for the assessment of limbal ischemia. To visualize the limbal vasculature, the device was focused manually to get a sharp image for all 4 quadrants of the limbus. The absence of vasculature was regarded as "ischemia," and the amountof the ischemia was defined in clock hours. The limbal ischemia detected in clinical evaluation with biomicroscopy was compared with the AS-OCTA detected ischemia amount to make a conclusion for the correlation. Nineteen eyes of 18 patients with acute ocular surface chemical injury were enrolled to the study (2 female, 16 male). The mean age was 35.1 ± 10 (18-55), and the mean best corrected visual acuity was 0.75 ± 1 (0.1-3.1) LogMAR. The causative agents were acid in 6 and alkaline in 12 patients. Limbal ischemia detected by using AS-OCTA was greater ((5.8 ± 2.6 (2-10) clock hours) than that detected in biomicroscopy (4.8 ± 2.4 (2-12) clock hours). The difference was statistically significant (p < 0.0005). AS-OCTA has a significant importance on limbal vascularity visualization; therefore, its use for more objective and sensitive evaluation of limbal ischemia in ocular surface chemical injuries seems to have a crucial impact. AS-OCTA images may reveal the extension of limbal ischemia more precisely than clinical evaluation with biomicroscopy. However, future studies with higher number of patients are needed to come to a specific conclusion.

The Boston Keratoprosthesis-The First 50 Years: Some Reminiscences.

Millions of people worldwide are bilaterally blind due to corneal diseases including infectious etiologies, trauma, and chemical injuries. While corneal transplantation can successfully restore sight in many, corneal graft survival decreases in eyes with chronic inflammation and corneal vascularization. Additionally, the availability of donor cornea material can be limited, especially in underdeveloped countries where corneal blindness may also be highly prevalent. Development of methods to create and implant an artificial cornea (keratoprosthesis) may be the only option for patients whose eye disease is not suitable for corneal transplantation or who live in regions where corneal transplantation is not possible. The Boston Keratoprosthesis (B-KPro) is the most commonly implanted keratoprosthesis worldwide, having restored vision in thousands of patients. This article describes the initial design of the B-KPro and the modifications that have been made over many years. Additionally, some of the complications of surgical implantation and long-term care challenges, particularly complicating inflammation and glaucoma, are discussed.

Deep anterior lamellar limbo-keratoplasty for bilateral limbal stem cell deficiency with corneal scarring in chemical injury sequelae: Two case reports.

Introduction and importanceThis report describes a new technique of deep anterior lamellar limbo-keratoplasty for the management of bilateral limbal stem cell deficiency (LSCD) with corneal scarring.Presentation of casesA 45-year-old male presented with chronic sequelae of ocular chemical injury and had bilateral total LSCD with corneal scarring. The visual acuity (VA) in the right eye was counting fingers. A large diameter deep anterior lamellar limbo-keratoplasty (DAL-LK) was carried out and the donor cornea and limbus were sourced from a single tissue. The VA at the last visit, 2.5 years after the surgery was 20/80. A similar presentation was seen in a 31-year-old male with a VA of 20/320 in the right eye. He underwent a DAL-LK and 3 years after the procedure, the VA was 20/60. Both grafts remained clear with no episodes of rejection until the last follow up visit.DiscussionLimbal stem cell transplantation with keratoplasty or a keratoprosthesis is required to manage bilateral LSCD with stromal scarring. The former necessitates multiple interventions while the latter is associated with several globe threatening complications. DAL-LK was devised to overcome these disadvantages and offers a simple, single staged technique of simultaneously transplanting the corneal stroma with the limbal stem cells. As the host endothelium is preserved, there is no risk of rejection episodes.ConclusionDAL-LK can successfully restore stability of the ocular surface and visually rehabilitate cases with bilateral LSCD and stromal scarring. The procedure has stable long-term outcomes with a good safety profile.

Eye injuries: Understanding ocular trauma.

Ocular trauma may result in pathology ofthe ocular surface and adnexa, extraocular muscles, orbital walls, eyeandoptic nerve. Detailed history followed by a complete ocular and, ifindicated, radiological examination istherefore a crucial part of any trauma assessment. Itis important to determine whether a patient with ocular trauma can be reassured or requires immediate referral for further investigation and surgical repair. This review examines chemical eye injuries, orbital fractures, superficial corneal foreign bodies, closed globe injury and suspected open globe injury with orwithout intra-ocular foreign bodies. Astructured approach to the history and examination is provided. The aim of this article is to enhance clinician confidence when encountering these patients. Ocular trauma can lead to serious sight‑ and eye-threatening consequences. Accurate history-taking and astute observation are paramount for timely treatment or referral that may prevent blindness. This review discusses the management and referral pathways for common presentations of ocular trauma.

Supporting discovery and development of medical countermeasures for chemical injury to eye and skin

Vesicants, from vesica (Latin for blister), can cause local and systemic toxicity. They include the chemotherapy drug nitrogen mustard and chemical warfare agents sulfur mustard, Lewisite, and phosgene oxime. These agents are commonly released in vapor form and consequently, eyes and skin are the most vulnerable. The ocular and cutaneous injuries can be acute, subacute, or chronic, and can predispose casualties to secondary deleterious effects. Underlying these broad organ responses are shared and tissue-specific cellular and molecular biological cascades that attempt to counteract such chemical injuries. Depending on the severity of the chemical insult, biological responses often lead to inadequate wound healing and result in long-term pathology instead. Exposure to other toxic industrial chemicals such as acrolein, chloropicrin, and hydrogen fluoride, can also cause prominent eye and skin damage. There are currently no FDA-approved drugs to counteract these injuries. Hence, the possibility of a mass casualty emergency involving these chemicals is a major public health concern. Recognizing this critical challenge, the United States Department of Health and Human Services (HHS) is committed to the development of medical countermeasures to advance national health and medical preparedness against these highly toxic chemicals. Here, we provide an overview of various HHS funding and scientific opportunities available in this space, emphasizing parallels between eye and skin response to chemical injury. We also discuss a main limitation of existing data and suggest ways to overcome it.

Abstract 5431: FGF7 peptide (FGF7p) mimetic mitigates urothelial injury from cyclophosphamide or radiation

Abstract Background: Hemorrhagic cystitis from chemical or radiation bladder injury can cause life-threatening bleeding and/or urothelial cancer. Fibroblast growth factor 7 (FGF7) blocks urothelial apoptosis induced by cyclophosphamide (CPP) or radiation, but there are limitations to its use in patients, including an inability to apply it by direct bladder infusion and the high cost due to the large size of the protein (18.8 kDa). Purpose: We previously identified a small peptide that equaled or exceeded the mitigation effect of full length FGF2 on acute radiation syndrome. Benefits of developing a similar FGF7 biomimetic peptide include lower likelihood of inflammation, longer shelf life, higher purity, and lower cost than the full-length protein. Methods: Based on the 3-dimensional structure of FGF2 peptide, a corresponding FGF7 peptide (FGF7p) consisting of 19 amino acids was identified and synthesized. FGF7p or vehicle was given subcutaneously (SQ) to female mice subjected to sham injury, intraperitoneal (IP) CPP or external beam radiation over the bladder. One day after injury, bladders were harvested. Slides with paraffin embedded tissues underwent H&amp;E staining, TUNEL and immunofluorescence (IF) assays. Results: In uninjured control mice, a 20 mg/kg threshold dose of FGF7p induced expression of phosphorylated (activated) FRS2a (pFRS2a) and pAKT in urothelium (consistent with cytoprotective effects of FGF7, albeit at a 4x higher dose than full length FGF7). Unexpectedly, FGF7p activation of urothelial FRS2a and AKT was delayed 24 hours compared to FGF7. FGF7p (20 mg/kg) or vehicle was given at 72 and 48 hours prior to CPP (150 mg/kg). One day after CPP, TUNEL staining revealed an increase in apoptotic and sloughing urothelial cells in vehicle-treated mice compared to in FGF7p-treated mice. IF for pAKT and its targets, pS6K and pBAD, revealed minimal staining in vehicle-treated mice, but strong urothelial staining for all protective markers in FGF7p-treated mice. Using the same dosing strategy, we subjected anesthetized mice to 10 Gy radiation over the bladder. One day after injury, TUNEL staining revealed many more apoptotic urothelial nuclei in control mice, than in FGF7p-treated mice. Conclusions: FGF7p appears to block bladder urothelial apoptosis via AKT and its targets, in a similar manner to FGF7. FGF7p is 200x less expensive to make vs. FGF7 and is likely to work via direct bladder infusion (avoiding systemic side effects) due to its small size. Our future studies will clarify long term benefits of FGF7p and assess effectiveness of direct bladder infusion. Citation Format: Sridhar Narla, Lori P. Rice, David Ostrov, Steven G. Swarts, Dietmar W. Siemann, Carlton M. Bates. FGF7 peptide (FGF7p) mimetic mitigates urothelial injury from cyclophosphamide or radiation [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 5431.