Chemical Composition Of Elephant Grass Research Articles

Mesquite pods as a source of soluble carbohydrates in elephant grass silages

The objective of this study was to evaluate the fermentation parameters and chemical composition of elephant grass silage with inclusion of crushed mesquite pods, at the levels of 8, 16, 24 and 32% as a source of soluble carbohydrates, in addition to a control treatment (no mesquite pod). Fermentation parameters were evaluated for gas losses (GL), effluent losses (EL), dry matter recovery (DMR), pH, ammonia nitrogen (NH3-N), and chemical composition related to dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEM), cellulose (CEL), lignin (LIG) and total digestible nutrients (TDN). A quadratic behavior (p<0.05) was found for GL, with a reduction of 45.58% with 32% inclusion. There was a decrease (p<0.05) in EL (59.22%) and NH3-N (38.21%) and an increase in DMR by 17.57% as the mesquite pod meal was added to the silage. As for the chemical composition, the inclusion of mesquite pod meal promoted increments (p<0.05) of 72.28%, 69.58% and 29.80% for the DM, CP and TDN, respectively, and a decrease in NDF (29.92%) and ADF (31.84%) up to the highest level of inclusion of mesquite pod meal (32%). The inclusion of up to 32% mesquite pod meal in elephant grass silage promotes better fermentation stability in the silo, by reducing losses and increasing the DM content of the ensiled mass, which minimizes nutrient leaching. The use of mesquite pod meal as a source of soluble carbohydrates can also improve the silage chemical composition, increasing the content of CP and TDN, and reducing the content of NDF, ADF and Lignin.

Effects of eucalyptus wood (Eucalyptus globulus) ash on biomass yield and nutritive value of elephant grass (Pennisetum purpureum) at Habru District, North Wollo Zone, Ethiopia

The objective of this study was to determine the biomass yield and chemical composition of Elephant grass (Pennisetum purpureum) at two successive harvests using five levels of eucalyptus (Eucalyptus globulus) ash as a fertilizer. Thus, a 2 x 5 factorial experiment was used in a randomized complete block design (RCBD) with four replications. The factors considered were successive harvests (first harvest performed after 90 days of planting and second harvest after 70 days of first harvest), and five levels of eucalyptus ash (0.0, 0.5, 1.0, 1.5 and 2.0 kg/m2). The highest plant height (2.5 ± 0.14 m) was recorded on plots to which a 2.0 kg/m2 eucalyptus ash was applied. The highest mean value of tiller numbers per plant (41± 1.5), green biomass yield (GB) (5.8 ± 0.2 MT/ha) and CP contents (8.85 %) were significantly increased with an increase in the level of eucalyptus ash with increasing successive harvests. The highest mean dry matter yield (DMY) 6.2 ± 0.16 was attained at the first harvest which receives 2.0 kg/m2 of eucalyptus ash. The highest mean value of ash (20.9 ± 8.4) was recorded at the two higher ash levels at the first harvest. However, the lowest mean value of NDF (53.3 ± 3.35) and ADF (40 ± 0) were recorded at the second harvest with the highest ash levels. All the fiber contents of the forage grass decreased with increase in successive harvests and amount of ash levels. It can be concluded that the second harvest and application of 2.0 kg/m2 of eucalyptus ash improved yield and nutritive value of elephant grass.

Effects of inclusion of patauá, Oenocarpus bataua meal on elephant grass silage

ABSTRACT Elephant grass silages have high moisture content, causing nutrient loss, mainly by effluent production. The use of moisture absorbent additives, such as patauá, Oenocarpus bataua meal, can be a strategy to reduce losses in these silages. The aim of this study was to determine the fermentation losses, microbiological composition, fermentative characteristics, and chemical composition of elephant grass silage with increasing levels of inclusion of patauá meal. The experiment was conducted in a completely randomized design, with four concentrations of patauá meal (7%, 14%, 21%, and 28%, based on fresh matter) and a control without patauá, and five replicates. The elephant grass was collected manually after 60 days of growth. After chopping, the grass was homogenized, mixed with the patauá meal according to the established treatments, and ensiled in experimental silos of 15 L. The inclusion of patauá meal in elephant grass silage had positive effects on the chemical composition and effluent loss. It significantly increased dry matter, organic matter, crude protein, nitrogen insoluble in neutral detergent and acid, ethereal extract, lignin, and non-fibrous carbohydrates of the silages. It also reduced effluent losses, neutral and acid detergent fiber contents, and cellulose. Our findings contribute towards reducing the environmental impact caused by the production of effluents during ensilage of tropical grasses and providing a potential use for the residue from the production of patauá oil, thus avoiding disposal in the environment.

Fermentation characteristics and chemical composition of elephant grass silage with ground maize and fermented juice of epiphytic lactic acid bacteria

This study aimed to evaluate the microbial populations, fermentation profile, dry matter recovery and chemical composition of elephant grass silage with ground maize (GM) and the fermented juice of epiphytic lactic acid bacteria (FJLB). A factorial design was used with four levels of GM (0 g/kg, 50 g/kg, 100 g/kg and 200 g/kg), untreated or treated with FJLB, in a completely randomized design with five replicates. A pre-experiment was undertaken to determine the optimum level of sucrose to be added to fermented juice for the development of epiphytic microflora. In this pre-experiment, a completely randomized design with three replications was used. The treatments were represented by the levels of sucrose (0 g/kg, 5 g/kg, 10 g/kg, 20 g/kg, 40 g/kg, 60 g/kg and 80 g/kg, fresh matter basis). The microbial populations, dry matter recovery, and effluent losses were affected by the interaction between GM and FJLB. Dry matter (DM) and crude protein (CP) contents increased linearly with the inclusion of GM. The addition of GM enhanced the fermentation process via a reduction in losses, and improvements were identified in the nutritional value of elephant grass silages. The use of fermented juice increased dry matter recovery, and its effect was more pronounced when ground maize was added. Keywords: dry matter recovery, lactic acid, microbiology, Pennisetum purpureum

Fermentative losses and chemical composition of elephant grass silage added with castor bean hull

Fermentation and chemical composition of elephant grass silage was evaluated with 0, 10, 20 and 30% castor been hull based on the natural matter. The experimental design was completely randomized with five replications. Silos were weighed out during manufacture, and reweighed before and after the opening after storage for 30 days. We determined the chemical composition, specific mass and effluent and gas losses, dry matter recovery, pH and ammonia nitrogen of the silage. The specific mass, pH, dry matter content, crude protein, acid detergent fiber, total carbohydrates and cellulose increased linearly (P<0.05) with the inclusion of castor bean hull. Quadratic effect was observed for effluent and gas losses, ammonia nitrogen and non-fibrous carbohydrates with minimal point when the castor bean hull was included at 25.63; 31.19; 28.07 and 11.7%, respectively. Dry matter recovery and neutral detergent fiber adjusted to a quadratic equation with maximum point when the castor bean hull was added at 24.20 and 20.62%, respectively. The inclusion of castor bean hull in elephant grass silage reduces losses during fermentation without compromising the chemical composition when included in the ratio of up to 25%, based on the natural matter.

Parâmetros fermentativos e composição química de silagem de capim elefante cv. Roxo com adição de vagens de faveira

The objective of this study was to evaluate the fermentative parameters and chemical composition of elephant grass silages with the addition of faveira pods. The pods were added to the forage at levels of 8, 16 and 24% on a dry matter (DM) basis, together with the control treatment (0%), in a completely randomized design. The pH, losses through effluent and gas, dry matter recovery, crude protein (CP), neutral detergent fiber (NDF), acid detergent (ADF), lignin and acid detergent insoluble nitrogen (ADIN) were quantified, and cellulose and hemicellulose were estimated. There was a linear increase (P<0.05) in losses through gas and a reduction in effluent production (1.17 kg/ton DM) at 24%, with pH remaining at the ideal level (3.76). Regarding the chemical composition of silage, an increase in DM and CP (6.53%) was observed at the highest levels of pod inclusion, with lower proportions of NDF (52.99%) and ADF (36.48%). However, ADIN levels increased with increasing pod levels in the silage (+ 6.81%). Faveira pods up to 24% are indicated as additives for elephant grass silage, as they improve silage quality, fermentation and chemical composition.

Agronomic and nutritional responses of Carajas elephant grass fertilized with protected and non-protected urea

The objective of this study was to assess the agronomic and nutritional responses of the elephant grass cultivar Carajas when subjected to fertilization with protected and conventional urea. A randomized block design with 4×2+1 factorial arrangement was adopted. The treatments consisted of four doses (100, 200, 400, and 800 kg N ha-1), two nitrogen sources (conventional and protected urea), and one additional treatment without fertilization. The experiment was carried out from October 2015 to March 2016, in a semi-arid region with a dystrophic yellow latosol type soil. The application of conventional urea was done with each of the multiple cuts performed per year, whereas the protected urea was applied in a single dose. Total senescence rate (TSR), leaf and stem elongation, height, tiller density, total forage biomass, green leaf and stem biomass, and dry matter all increased with increasing N doses. However, the phyllochron, live material/dead material ratio, and agronomic efficiency all decreased with N increasing dose. Plants fertilized with protected urea presented greater TSR and phyllochron. Mineral matter content was higher in plants fertilized with conventional urea. Neutral detergent fiber content was maximized at a dose of 437.63 kg N ha-1. The protected urea resulted in a higher value of leaf/stem ratio at the 400 kg N ha-1 dose, whereas ether extract and crude protein at the 800 kg N ha-1. With application of conventional urea, the highest crude protein value occurred at a dose of 100 kg N ha-1. The morphogenesis, structure, production, and chemical composition of elephant grass ‘Carajas’ were all positively influenced when nitrogen doses were increased, with the recommended optimal dose for use being 400 kg N ha-1 for both sources. The use of protected urea is recommended because of its slow release and need for only a single application.

Impacts of citrus pulp addition and wilting on elephant grass silage quality

The objective of this work was to evaluate the use of citrus pulp addition and wilting on fermentative characteristics, chemical composition as well as gas and effluent losses of elephant grass silage. The elephant grass (70 growth days) was collected manually; one portion was immediately chopped and another one was allowed to wilt in the sun for a period of 6 h and subsequently chopped for the production of silage. Experimental PVC silos (10 cm diameter x 30 cm height) were used and sand bags were placed at the bottom of the silos. Citrus pulp (80 g kg-1 of MN) was added at the beginning of ensilage. The ensiled material was manually compressed to provide a specific mass of approximately 600 kg m-3 of silage. After 60 days, the silos were opened; gas and effluent losses were calculated and we determined pH, dry matter (DM), crude protein(CP), neutral detergent fibre (NDF), lignin, ammonia nitrogen, ash and in vitro dry matter digestibility (IVDMD). We added 0 or 80 g kg-1 of citrus pulp to wilted and un-wilted elephant grass. We used a completely randomised design with a 2 x 2 factorial arrangement (wilted or un-wilted) x (with or without citrus pulp), totalling treatments with five repetitions. Average values were compared using the F test with a probability of 5%. The addition of citrus pulp resulted in reduced levels of NDF, lignin, ash, N-NH3 and pH and in increased values of DM and IVDMD of silages. Wilting increased the DM, NDF and lignin values and reduced the concentrations of CP, IVDMD and N-NH3. Based on our results, citrus pulp addition improves the chemical composition of elephant grass silage and increases its in vitro dry matter digestibility.

Rendimento de hidrólise e produção de etanol lignocelulósico a partir de biomassa de capim elefante

Due to search for alternative biomasses, that are easy to access, low cost and high in cellulose concentration, for the production of 2nd generation ethanol - or lignocellulosic - this study was carried out with Pennisetum purpureum, known as elephant grass. The study was conducted to determine the chemical composition of elephant grass forage to evaluate its use in the production of lignocellulosic ethanol. The elephant grass stems were reaped in the experimental area of Feira Agrotecnologica de Palmas (AGROTINS) on august 2015. The results have shown that the elephant grass presented approximately 60% cellulose, demonstrating the potential use of this biomass to obtain reducing sugars. The biomass underwent chemical pretreatment before the enzymatic hydrolysis step using commercial cellulase enzyme Cellic CTEC2. After hydrolysis, gave a 54% yield of reducing sugars, which has shown a yield of 53,9% in the hydrolysis. In addition, the yield for ethanol production was 87,8%. Due to this, it is possible to assure that the elephant grass biomass has a great potential as cellulose source, therefore it can be used as an alternative that is cheap and huge in availability to the production of lignocellulosic ethanol.

Chemical composition of elephant grass silages supplemented with different levels of dehydrated cashew bagasse

The objective of the present study was to evaluate the chemical composition of elephant grass silages supplemented with different levels dried cashew bagasse (DCB). Our experiment used a randomized design replicated four times, each replicate consisting of the following five treatments: 100% elephant grass; 95% elephant grass + 5% DCB; 90% elephant grass + 10% DCB; 85% elephant grass + 15% DCB; and 80% elephant grass + 20% DCB. The elephant grass was cut manually to a residual height of 5 cm at 80 days of age, and cashew bagasse was obtained from the processing of cashew stalks used in fruit pulp manufacturing in Mossoró/RN. Plastic buckets were used as experimental silos, and 90 days after ensiling the experimental silos were opened and the contents analyzed. The addition of dried cashew bagasse to silage linearly increased the levels of dried matter and crude protein by 0.59% and 0.13%, respectively, for each 1% addition (P &lt; 0.05). The neutral detergent fiber and acid detergent content of the silages was reduced by 0.22% and 0.09%, respectively, for each 1% addition of the bagasse. The total carbohydrate content was not influenced by the bagasse addition (P &gt; 0.05), and averaged 82.29%. The levels of non-fiber carbohydrate showed linear growth (P &lt; 0.05) as the dehydrated cashew bagasse was added, and pH and ammoniacal nitrogen levels were reduced. The addition of the dehydrated bagasse to elephant grass silage improves its chemical composition, and it can be effectively added up to the level of 20%.

Silage fermentation and chemical composition of elephant grass inoculated with rumen strains of Streptococcus bovis

The objective of this study was to evaluate the effects of Streptococcus bovis JB1 and HC5 on the fermentation and chemical composition of elephant grass ensiled in laboratory silos. A complete randomized design was used with four treatments: elephant grass silage, elephant grass silage inoculated with 106CFU/g S. bovis JB1 strains; elephant grass silage inoculated with 106CFU/g S. bovis HC5 strains; elephant grass silage inoculated with 106CFU/g Enterococcus faecium, and six replications. The highest number of enterobacteria, fungi and yeast occurred in the control and elephant grass silages inoculated with E. faecium (P<0.05). The silage inoculated with Streptococcus bovis JB1 and HC5 presented the lowest enterobacteria, fungi and yeast populations and the highest number of lactic acid bacteria, 10.03 and 9.97logCFU/g, respectively. The pH and ammoniac nitrogen values were low (P<0.05) in the treatments with Streptococcus bovis JB1 and HC5, respectively. The observed pH values were 3.83 and 3.96 in the treatments with S. bovis JB1 and HC5, respectively, lower than the observed value of 4.01 in the control treatment. The silages inoculated with S. bovis JB1 and HC5 presented the highest (P<0.05) lactic acid values, 67.8 and 66.2g/kg DM, respectively, and the lowest acetic, butyric and propionic acid values. Inoculation with Streptococcus bovis resulted in a higher (P<0.05) crude protein content in the silages, highest dry matter and crude protein recovery and lowest losses through gases and effluents. Treatments with S. bovis JB1 and HC5 presented values of 93.94 and 92.45 for dry matter recovery. There were no statistical differences (P>0.05) for the other chemical characteristics. Both S. bovis JB1 and HC5 improved the fermentation profile, reduced nutrient losses and increased the nutritional value of the elephant grass silage.

Silagem de capim-elefante aditivada com produtos alternativos

The objective was to evaluate the fermentation pattern and chemical composition of elephant grass silage using different additives. The experimental design was completely randomized with six treatments (elephant grass no additive; with 100 kg of rice bran ton-1 of green mass (GM); with 100 kg of soybean hulls ton-1 GM; with 100 kg of corn meal ton-1 GM; with 200 kg of sugar cane ton-1 GM; with inoculants) and four replications. PVC silos were used, adopting a density of 600 kg of green mass m-3. With the exception of inoculant, all additives used resulted in increased dry matter content (27.95 to 31.65%) and soluble carbohydrate contents (15.20 to 17.14%) in the forage being ensiled. The additives promoted an adequate fermentation pattern for pH values (3.70 to 3.96) and ammonia nitrogen (3.67 to 4.44% of total N) observed in the silage. The rice bran additive yielded silage with higher contents of crude protein (6.41%) and total digestible nutrients (61.91%) compared to the treatment without any additive.

Predição do consumo de pasto de capim-elefante (Pennisetum purpureum, Schumack) por vacas mestiças Holandês x Zebu em lactação

Foram desenvolvidas equações de predição de consumo de pasto de capim-elefante (Pennisetum purpureum, Schumack) por vacas mestiças Holandês x Zebu em lactação, utilizando-se procedimentos de stepwise em regressões múltiplas, aplicados a um banco de dados de experimentos conduzidos ao longo de três anos na Embrapa Gado de Leite (Coronel Pacheco, MG). As variáveis independentes disponíveis foram relacionadas a características inerentes às vacas (dias em lactação; teores de proteína, gordura e extrato seco total e produções destes componentes no leite; produção de leite in natura ou corrigida para 4% de gordura; ordem de lactação; peso vivo atual; peso vivo ao parto e grau de sangue Holandês x Zebu); ao manejo (dias de pastejo; disponibilidade de forragem e período de descanso da pastagem); ao ambiente (estação do ano e precipitação pluviométrica) e à alimentação (digestibilidade in vitro e parâmetros da composição química do pasto de capim-elefante e da cana-de-açúcar - Saccharum officinarum (L.) corrigida com 1% de uréia; consumos de suplemento volumoso (cana corrigida com uréia) e concentrado; concentrações fecais de proteína bruta e de fibras em detergente neutro e ácido). Efeitos linear e quadrático e transformações logarítmicas foram adicionalmente incluídos no banco de dados. Foram obtidas equações de predição de consumo de pasto de capim-elefante (expresso em kg/vaca/dia ou % do peso vivo) com coeficientes de determinação de 65,2 a 67,0%. As principais variáveis independentes incluídas nas equações foram o consumo do suplemento volumoso usado na estação seca do ano (cana corrigida com uréia); a digestibilidade in vitro do pasto de capim-elefante; a precipitação pluviométrica; a produção de leite corrigida para 4% de gordura; o peso vivo atual ou, em alternativa a este, o valor da pesagem realizada após o parto da vaca; além do consumo de suplemento concentrado, que evidenciou um efeito de substituição àquele do pasto de capim-elefante.

Efeito de aditivos biológicos comerciais na silagem de capim-elefante (Pennisetum purpureum Schum)

Avaliaram-se o pH e a composição bromatológica de silagens de capim-elefante var. Napier, sem e com três aditivos biológicos comerciais (B, C e D) em duas concentrações de diluição, a recomendada pelo fabricante (concentração 1) ou com o dobro (concentração 2), feitas em microsilos de PVC. O delineamento experimental foi inteiramente ao acaso, com sete tratamentos e seis repetições por tratamento. Apenas o aditivo B em ambas as concentrações (3,73; 4,07) foi eficiente em reduzir (P&lt;0,05) o pH em relação ao tratamento-controle (5,01). A porcentagem de matéria seca aumentou (P&lt;0,05) com o uso do aditivo B, nas concentrações 1 (29,9) e 2 (31,3), e dos aditivos C e D, na concentração 2 (26,8; 25,7). Quanto à proteína bruta, somente houve diferença (P&lt;0,05) entre o tratamento-controle (6,6) e os dois tratamentos com aditivo B (13,4; 12,09). As frações de fibra em detergente neutro e lignina foram menores (P&lt;0,05) nos tratamentos com aditivo B em relação ao tratamento-controle. A duplicação da dose do aditivo B não aumentou sua eficiência. Nos macro-elementos cálcio e fósforo das silagens, todos os aditivos elevaram a concentração de cálcio e não alteraram a de fósforo. Concluiu-se que o aditivo B, constituído de Lactobacillus plantarum e Pediococcus acidilactici, melhorou tanto a qualidade de fermentação quanto o valor nutritivo da silagem de capim-elefante, e que a duplicação da concentração dos produtos não aumentou (P&lt;0,05) a qualidade das silagens.

Composição química do capim-elefante cv. Roxo cortado em diferentes alturas

O objetivo deste trabalho foi verificar o efeito dos cortes efetuados a 0, 15, 30 e 45 cm sobre a composição química do capim-elefante (Pennisetum purpureum, Schum.) cv. Roxo, em épocas seca e chuvosa. As amostras foram obtidas de uma área útil de 8,4 m² de cada parcela. Após o corte de uniformização, efetuaram-se dois cortes no período seco, em intervalos de 90 dias, e três no período chuvoso, em intervalos de 60 dias. De cada parcela foi tomada uma amostra de 3 a 5 perfilhos, desidratada em estufa e triturada para analises laboratoriais. Foram avaliados os percentuais de matéria seca (MS), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), celulose (CEL), hemicelulose (HCEL), lignina (LIG) e cinzas. As alturas de corte não influenciaram a composição química da forrageira, nem houve interação com as épocas. Com exceção de hemicelulose e cinzas, os cortes na época seca mostraram resultados superiores à chuvosa. As médias nas duas épocas foram 19,70 e 17,44% para MS; 7,74 e 7,25% para PB; 76,41 e 71,13% para FDN; 42,75 e 41,02% para FDA; 31,44 e 30,43% para CEL; 30,66 e 30,28% para HCEL; 9,25 e 7,83% para LIG; e 1,97 e 3,38% para cinzas, nos períodos seco e chuvoso, respectivamente.