AbstractStream nitrate levels in a small catchment of mixed land use (the Slapton Wood catchment) have been studied since September 1970; a record of this length is possibly unique in the United Kingdom for such a small basin (94 ha). A sustained increase in nitrate concentration has been observed during the study period. In addition to this long‐term trend, short‐term changes in nitrate concentrations relate to stream discharge levels and to seasonal variations. Multivariate statistical analysis has been used to quantify these trends and to identify those factors controlling the production and loss of nitrate from the catchment system. The main period of nitrate removal occurs in winter when high concentrations coincide with the main period of throughflow generation. The influence of climatic variability is illustrated by reference to the 1975/76 drought and post‐drought period.