We experimentally demonstrate a 100-km-reach, channel-reuse wavelength-division-multiplexing passive optical network (WDM-PON) with 40 Gb/s/λ downstream and 10 Gb/s/λ upstream transmission and a back-reflection optical signal detection-based automatic wavelength control method for a tunable laser used in a colorless optical network unit. Channel-reuse, full-duplex, 40 Gb/s/λ downstream and 10 Gb/s/λ upstream transmission on a 100 GHz WDM grid with 100 km reach was achieved. The transmission performance was measured using different optical signal to back-reflection optical signal ratios, optical signal to Rayleigh backscattering noise ratios, and different central wavelength shifts between the upstream and downstream signals in the channel-reuse system.