Centella Asiatica Research Articles

Comparative Evaluation of Efficacy of Chlorhexidine with Centella asiatica in the Management of Stage 2 Grade B Periodontitis in Type 2 Diabetes Mellitus Patients − A Randomised Controlled Clinical Trial

Introduction: Chemical plaque control agents are used as an adjunct to non-surgical periodontal therapy. Adjunctives can assist in proper oral hygiene maintenance. Type 2 diabetes mellitus is a metabolic disease that can reflect in the periodontium. Periodontal disease prevention and management in Type 2 diabetes patients need routine dental care. Over counter mouthwashes used by patients can result in side effects; this has popularised the use of herbal mouthrinses that have minimal side effects. Aim: The present study aimed at evaluating the efficacy of chlorhexidine (CHX) with Centella asiatica in the management of Stage 2 Grade B periodontitis in type 2 diabetes mellitus patients. Materials and method: Seventy Type 2 diabetes mellitus patients with periodontitis were recruited for this study. In Group A, 35 subjects received scaling and root planing (SRP) and 10% C. asiatica mouthwash. In Group B, 35 subjects received SRP and 0.2% CHX mouthwash (Colgate-plax, Colgate-Palmolive). Clinical periodontal outcomes measured were plaque index (PI), gingival index (GI), bleeding on probing (BOP) and probing depth (PD) at baseline, 1 month and 3 months post therapy. Salivary total antioxidant capacity (TAOC) and Interleukin 1β levels were also measured at baseline and 3 months. Results: Clinical parameters PI, GI, BOP and PD were reduced significantly in groups A and B, post-treatment, 3-month follow-up. However, the intergroup comparison didnot show any significant difference. Salivary TAOC levels were significantly increased in both groups after 3 months therapy. The intergroup comparison of salivary TAOC levels between groups didnot show any significant difference. Salivary interleukin 1β levels were significantly reduced in both groups post-treatment. Conclusion: This study suggests the anti-inflammatory and antioxidant potential of C. asiatica mouthwash and a safe alternative to CHX mouthwash as an adjunct in the management of Stage 2 Grade B periodontitis in type 2 diabetes mellitus patients.

Formulation and Performance of Polyherbal Antidandruff Hair Gel: A Multifaceted Solution

ABSTRACT Background: Wet-looking hairstyles that last for hours can be achieved using anti-dandruff hair gel. Natural components are included in the unique recipe of the gel to aid in dandruff treatment. Anti-dandruff hair gel is a preparation with antibacterial and antifungal activity that reduces dandruff and improves hair health. Materials and Methods: Five different formulations contain effective ingredients, such as Linum usitatissimum, Ocimum tenuiflorum, Lawsonia inermis, Tinospora cordifolia, and Centella asiatica, which exert their effects by showing antibacterial and antifungal activity. Results: The prepared hair gel was evaluated using parameters such as color, odor, homogeneity, texture, pH, viscosity, spreadability, washability, extrudability, stability, zone of inhibition, and in vitro skin irritation tests. By considering all the parameters, we conclude that our formulations exhibit better anti-dandruff activity. The overall experiment showed that the F2 formulation had more antibacterial activity and the F3 formulation had antifungal activity. Conclusion: The prepared anti-dandruff hair gel effectively combats the problem. Therefore, this hair gel may be preferable to other standard hair-setting or styling gels.

Study on hydrogel eye mask with Centella asiatica L and Aloe vera L extract

Study on hydrogel eye mask with Centella asiatica L and Aloe vera L extract

Amelioration of age-related cognitive decline and anxiety in mice by Centella asiatica extract varies by sex, dose and mode of administration.

Background: A water extract (CAW) of the Ayurvedic plant Centella asiatica administered in drinking water has been shown to improve cognitive deficits in mouse models of aging and neurodegenerative diseases. Here the effects of CAW administered in drinking water or the diet on cognition, measures of anxiety and depression-like behavior in healthy aged mice are compared. Methods: Three- and eighteen-month-old male and female C57BL6 mice were administered rodent AIN-93M diet containing CAW (0, 0.2, 0.5 or 1% w/w) to provide 0, 200mg/kg/d, 500mg/kg/d or 1,000mg/kg/d CAW for a total of 5 weeks. An additional group of eighteen-month-old mice were treated with CAW (10mg/mL) in their drinking water CAW for a total of 5weeks to deliver the same exposure of CAW as the highest dietary dose (1,000mg/kg/d). CAW doses delivered were calculated based on food and water consumption measured in previous experiments. In the fourth and fifth weeks, mice underwent behavioral testing of cognition, anxiety and depression (n = 12 of each sex per treatment group in each test). Results: Aged mice of both sexes showed cognitive deficits relative to young mice while only female aged mice showed increased anxiety compared to the young female mice and no differences in depression were observed between the different ages. CAW (1,000mg/kg/d) in the drinking water improved deficits in aged mice in learning, executive function and recognition memory in both sexes and attenuated the increased measures of anxiety observed in the aged female mice. However, CAW in the diet only improved executive function in aged mice at the highest dose (1,000mg/kg/d) in both sexes and did so less robustly than when given in the water. There were no effects of CAW on depression-like behavior in aged animals regardless of whether it was administered in the diet or the water. Conclusions: These results suggest that CAW can ameliorate age-related changes in measures of anxiety and cognition and that the mode of administration is important for the effects of CAW on resilience to these age-related changes.

Recent advancements in the role of phytochemicals and medicinal plants in prophylaxis and management of Alzheimer's disease.

Medicinal plants and phytochemicals are some of the major sources in the treatment of various neurodegenerative disorders including Alzheimer's disease (AD). There is no FDA-approved drug to target AD pathology directly. Full cognitive restoration and management of psychosis-like symptoms are still to be achieved. Being comparatively safer with fewer side effects, medicinal plants have been among the major areas of interest to be researched. Several mechanistic pathways are involved in AD including anticholinesterase activity, glutamate toxicity, free radicals generation, Amyloid β (Aβ) toxicity, inflammation, and mitochondrial dysfunction. Various phytochemicals such as paenol, andrographolide, isoquercitrin, flavonoids, and saponins obtained from different plant sources, various medicinal plants like Spirulina maxima, Salicornia europaea, Curcuma longa, Citrus Junos Tanaka, Cassiae semen, Centella asiatica as well as various traditional medicinal plants of China, Asia, Europe, Turkey, and Iran have been found effective against one or more of these targets. Large numbers of clinical trials are under process to evaluate the role of different phytoconstituents in AD management. Out of 143 agents under clinical trials, 119 have been categorized as disease-modifying agents. The present review extensively covers the recent advancements in the usage of phytochemicals and medicinal plants in various experimental AD models. It involves clinical trials and other research works divided into three sections, including those performed in vitro, in vivo, and in humans mainly from the last five years along with disease markers and mechanistic pathways involved. However, phytochemicals should be explored further in order to achieve neurorestoration in AD.

Centella asiatica effect on traumatic brain injury: A systematic review.

Mortality and morbidity in traumatic brain injury (TBI) cases remain a global problem. Various therapeutic modalities have been researched, including using herbal medicine. Centella asiatica has a lot of potential in neuropharmacology for various diseases. This systematic review aims to comprehensively review the currently available data about the impact of C. asiatica on TBI in a rat model. Systematic searches were conducted on PubMed, Scopus, and Google Scholar up to July 2023. This study follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses protocol. Researchers screened the titles and abstracts of all identified studies and then selected relevant studies through full-text reviews. Studies reported the effect of C. asiatica on animal model of TBI were included in the study. Data were extracted, and the result was reported using descriptive analysis. The risk of bias was evaluated using SYRCLE. Four studies met the inclusion criteria. One study highlighted the potential neuroprotective effects of Asiatic acid, one study explored spade leaf extract phytosome, while the rest used C. asiatica extracts. The primary findings of the included research revealed that C. asiatica might reduce oxidative stress, decrease neuronal apoptosis, have anti-inflammatory properties, alleviate neurological dysfunction, reduce cerebral edema, and boost cognitive performance in the TBI-induced rat's model. This review suggests that C. asiatica had the potential to benefit the TBI-induced rat model in terms of decreasing morbidity. Nevertheless, more studies are needed to perform a meta-analysis and ascertain the effects of C. asiatica on TBI in animal models.

Evidence of Potential Natural Products for the Management of Hypertrophic Scars.

Hypertrophic scarring is an aberrant wound-healing response to reestablish dermal integrity after an injury and can cause significant abnormalities in physical, aesthetic, functional, and psychological symptoms, impacting the patient's quality of life. There is currently no gold standard for preventing and treating hypertrophic scars. Therefore, many researchers have attempted to search for antihypertrophic scar agents with greater efficacy and fewer side effects. Natural therapeutics are becoming attractive as potential alternative anti-scarring agents because of their high efficacy, safety, biocompatibility, low cost, and easy accessibility. This review demonstrates various kinds of natural product-based therapeutics, including onion, vitamin E, Gotu kola, green tea, resveratrol, emodin, curcumin, and others, in terms of their mechanisms of action, evidence of efficacy and safety, advantages, and disadvantages when used as anti-scarring agents. We reviewed the literature based on data from in vitro, in vivo, and clinical trials. A total of 23 clinical trials were identified in this review; most clinical trials were ranked as having uncertain results (level of evidence 2b; n = 16). Although these natural products showed beneficial effects in both in vitro and in vivo studies of potential anti-scarring agents, there was limited clinical evidence to support their efficacy due to the limited quality of the studies, with individual flaws including small sample sizes, poor randomization, and blinding, and short follow-up durations. More robust and well-designed clinical trials with large-scale and prolonged follow-up durations are required to clarify the benefits and risks of these agents.

Study on the Soothing and Repairing Effects of Artemisia Annua Combined with Centella Asiatica Extract

Study on the Soothing and Repairing Effects of Artemisia Annua Combined with Centella Asiatica Extract

Potential of asiaticoside encapsulated alginate chitosan nanoparticles in the management of antiproliferative activity in C6 glioma cells

BackgroundAsiaticoside is a pentacyclic triterpenoid saponin constituent of the medicinal herb Centella asiatica, known for its neuroprotective, anticancer and other biological effects. The present study aims to develop and characterise asiaticoside encapsulated alginate chitosan nanoparticles (ACNPs) and evaluate their antiproliferative potential on C6 glioma cells. MethodsACNPs were prepared by the ionic gelation polyelectrolyte complexation technique and characterised by dynamic light scattering, scanning electron microscopy and transmission electron microscopy. Cytotoxicity and antiproliferative effect of ACNPs were studied on C6 glioma cells using MTT and EdU-assays. Apoptosis/necrosis in C6 glioma cells was determined by annexin V and acridine orange/ethidium bromide (AO/EtBr) assays. Intracellular ROS generation in C6 glioma cells exposed with ACNP was determined by DCFDA assay using flow cytometry. Cell cycle analysis in C6 glioma cells treated with ACNP was performed by flow cytometry. ResultsSynthesised ACNPs showed spherical shape with 200 nm particle size, good thermal stability, and an acceptable polydispersity index. ASI from synthesized ACNPs demonstrated 90% encapsulation efficiency (EE) and 10% drug release within 24 h. Upon ACNP treatment mBA cells exhibited good cell viability and intact cell morphology. However, C6 cells displayed dose-dependent cytotoxicity when treated with ACNP. Annexin V and acridine orange/ethidium bromide (AO/EtBr) assays revealed late apoptosis and necrosis (p < 0.05) in C6 glioma cells treated with ACNP. Likewise, ACNPs significantly augmented reactive oxygen species generation (p < 0.05) in C6 glioma cells. ACNP treatment also resulted in cell cycle arrest at the G1 phase with chromatin condensation in C6 glioma cells. ConclusionsThese findings suggest that ACNPs act as an antiproliferative agent against C6 glioma cells via an increased intracellular ROS pathway that promotes apoptosis/necrosis. This study throws light on more in-depth mechanistic aspects of managing brain disorders using C. asiatica and its phytoconstituents.

Mathematical Modelling on The Thin-Layer Drying of Centella Asiatica Leaves

The best drying conditions can be selected by using mathematical modeling of herb drying processes. Development of mathematical models for drying Centella asiatica leaves is the major goal of the current study. At temperatures of drying of 50, 55, and 60 C, the drying behavior of Centella asiatica leaves was examined. The drying data were fitted to the eight thin-layer models. Non-linear regression evaluates the accuracy with which validated mathematical models fit the data. By evaluating R2, reduced chi-square, and RMSE between the observed and predicted moisture ratios, the performance of various models was evaluated. The analysis of the drying curve of Cetella asiatica leaves revealed that the Midilli-Kucuk model was the most. accurate.

Standardized Centella asiatica extract ECa 233 alleviates pain hypersensitivity by modulating P2X3 in trigeminal neuropathic pain.

During oral surgery and temporomandibular joint repositioning, pain hypersensitivity often occurs due to irritation or inflammation of the nerve endings in the orofacial region. This study aimed to investigate the effects of ECa 233, a Centella asiatica-standardized extract, on the development of mechanical hyperalgesia and allodynia induced by chronic constriction injury of the infraorbital nerve in mice. The right infraorbital nerves of the mice were ligated. Oral carbamazepine (20 mg/kg) or ECa 233 (30, 100, or 300 mg/kg) was administered daily for 21 days. Von Frey and air-puff tests were performed on both sides of the whisker pad on days 0, 7, 14, and 21. Thereafter, the expression of purinergic receptor subtype 3 (P2X3) and voltage-gated sodium channel 1.7 (NaV1.7), a transmembrane protein, in the trigeminal ganglion and c-fos immunoreactivity-positive neurons in the trigeminal nucleus caudalis was assessed. After 21 days of infraorbital nerve ligation, the mice showed allodynia- and hyperalgesia-like behavior, P2X3 and NaV1.7 were upregulated in the trigeminal ganglion, and nociceptive activity increased in the trigeminal nucleus caudalis. However, the oral administration of carbamazepine (20 mg/kg), ECa 233 (100 mg/kg), or ECa 233 (300 mg/kg) mitigated these effects. Nevertheless, ECa 233 failed to affect NaV1.7 protein expression. Carbamazepine and ECa 233 can prevent pain hypersensitivity in mice. Considering the side effects of the long-term use of carbamazepine, ECa 233 monotherapy or combined ECa 233 and carbamazepine therapy can be used as an alternative for regulating the development of hypersensitivity in trigeminal pain. However, further detailed clinical studies should be conducted to provide comprehensive information on the use of ECa 233.

Production and Characterization of Nata from Yam Bean Juice using Komagataeibacter nataicola TISTR 975

This study aimed to produce nata from Komagataeibacter nataicola TISTR 975 using yam bean juice as the fermentation substrate. The chemical components of yam bean juice and the physical characteristics of the produced yam bean nata were investigated. Yam bean nata were processed with juices from lemongrass (Cymbopogon citratus, Stapf; LG), Gac fruit (Momordica cochinchinensis Spreng; GF), and Centella (Centella asiatica; CA) and subsequently subjected to CIELAB value analysis, texture profile analysis (TPA), and sensory evaluation. The wet weight and thickness of yam bean nata were recorded as 130.36 ± 0.45 g and 9.64 ± 0.50 mm, respectively. The color analysis of yam bean nata revealed that the L* value slightly tended toward brightness; the a* value was negative, indicating green; and the b* value shifted toward blue. Upon processing, the yam bean nata with LG, GF, and CA exhibited an L* value tending toward white, an a* value indicating green, and a b* value indicating yellow. TPA results revealed a statistically significant disparity in hardness among the processed yam bean nata. Sensory attribute assessment indicated that processing the yam bean nata with LG, GF, and CA juices increased the liking score for overall acceptability.

동물모델을 이용한 병풀의 항아토피 효능 평가

Centella asiatica (C. asiatica) is currently widely used in wound healing and has recently been reported to have&#x0D; immune suppressive properties. Atopic dermatitis (AD) is a chronic inflammatory and allergic disease. We herein&#x0D; investigated the anti-atopic effect of C. asiatica in vivo. To examine the anti-atopic dermatitis activity of C. asiatica the&#x0D; ovalbumin-induced BALB/c mouse model was used. Ovalbumin mixture was injected into the abdominal cavity and&#x0D; patched on the back to induce AD, and then treated by orally administering C. asiatica. C. asiatica reduced major signs&#x0D; of clinical skin severity such as erythema, pruritus, lichenification, edema/escoriations, and dryness. A decrease in the&#x0D; number of scratch was also seen in scratching counts. The level of serum IgE was also reduced in mice treated with C.&#x0D; asiatica. We therefore suggested C. asiatica extract can be used as a therapeutic agent for AD treatment.

병풀주정추출분말의 항염증 효능 검색

Centella asiatica (C. asiatica) is currently widely used in wound healing and has recently been reported to have immune suppressive properties. This study investigated the anti-inflammatory effect with ethanol-extract of C. asiatica cultivated in a smart farm. Analysis of asiaticoside in C. asiatica extract was performed using high performance liquid chromatography (HPLC) and as a result, the asiaticoside content of C. asiatica extract was 23.17 mg/g. The effect of C. asiatica extract on the expression of inflammatory mediators, such as interleukin-1beta (IL-1β), IL-6 and tumor necrosis factor-alpha (TNF-α), and nitric oxide (NO) was examined in murine macrophage cell line, RAW264.7 using ELISA method. C. asiatica extract inhibited the production of NO, IL-1β, IL-6, and TNF-α in macrophages stimulated with lipopolysaccharide (LPS) at a dose dependent manner. C. asiatica extract also suppressed the expression of IL-6 and IL- 8 in HaCaT cells, a human keratinocytes, stimulated with TNF-α/IFN-. These results suggest that C. asiatica cultivated in a smart farm can be effectively utilized as a therapeutic agent for inflammatory disease.

Total Phenolic Content and Antioxidant Activity of Pasteurized Milk With the Addition of Centella asiatica Leaf Extract

Milk has a high nutritional value. Minimal processing that can extend the shelf life of milk is pasteurization, but it can damage the nutritional content of milk. One of the additional efforts to improve milk quality and maintain the nutritional content of milk is to provide additional active compounds that act as antioxidants for milk consumers and the milk product itself. The use of Centella asiatica leaf extract as an ingredient is because there are at least 139 active compounds. This study aimed to study the total phenolic content and antioxidant activity of pasteurized milk with the addition of Centella asiatica leaf extract. The research was carried out in two stages, namely making gotu kola leaf extract and making pasteurized milk by adding gotu kola leaf extract concentrations of 0%, 0.25%, 0.5%, and 0.75%. Product analysis included total phenol analysis using Folin Ciocalteau and antioxidant activity using the DPPH method. Adding Centella asiatica leaf extract can increase total phenolic content and antioxidant activity. The total phenolic content obtained by adding Centella asiatica leaf extract at 0%, 0.25%, 0.50%, and 0.75% were 0.58 mgGAE/g, 1.93 mgGAE/g, respectively 2.33 mgGAE/g, and 3.41 mgGAE/g. At the same time, the results of antioxidant activity were 7.68%, 31.93%, 37.57, and 40.35%, respectively.

The effectiveness of topical gel formulation extract Centella asiatica and Curcuma domestica to fading striae gravidarum

Background: The incidence of striae gravidarum in Indonesia is 95%, and 79.7% are primigravida. Striae gravidarum is not a direct physical health risk but is associated with body image and decreased self-confidence. Curcuma domestica contains curcumin and vitamin C, which can stimulate the synthesis of type IV collagen. In contrast, the Centella asiatica plant contains triterpenoids and saponins, which can stimulate the synthesis of type I collagen, which helps regenerate skin cells and improve skin elasticity. However, it is necessary to see the effectiveness of the two herbs, Centella asiatica and Curcuma domestica, in fading striae gravidarum. Purpose: To prove the effectiveness of topical gel formulation extract Centella asiatica and Curcuma domestica for fading of striae gravidarum.Methods: This is a quasi-experimental study with a pretest-posttest control group design. The sample consisted of 32 primigravida pregnant women divided into two groups: the intervention group was given a combination of Curcuma domestica and Centella asiatica gel at a dose of 5 mg twice a day for 14 days, and the control group was given a mineral oil gel at a quantity of 5 mg twice a day for 14 days—measurement of striae gravidarum using Davey score. The statistical test used the Friedman and Wilcoxon test.Results: The average fading of striae gravidarum in the intervention group was more significant than in the control group, 11.44±4.131 to 4±3.033 vs.12.19±5.788 to 8.56±4.320 treatment on the 14th day. The analysis results showed that the topical gel formulation of Centella asiatica and Curcuma domestica was more effective in fading striae gravidarum than the placebo gel (p&lt;0.001).Conclusion: Administering topical gel containing 12% Centella asiatica extract and a 10% Curcuma domestica dose of 5 mg twice a day for 14 days affected the fading of striae gravidarum.

Quantitative Estimation of Variability in Content of Asiaticoside in Centella asiatica Linn Collected from Different Geographical Regions of India by HPLC

Centella asiatica Linn is a significant and highly valued medicinal plant in various indigenous system of medicine Ayurveda, Siddha and Unani Indian system of medicines and Chinese medicines. It is one of the chief herbs for treating skin disorder, wounds healing, gastrointestinal disorders, asthma, ulcers, leprosy, vein diseases and memory improvement. In the present study an attempt is made to develop HPLC method for qualitative and quantitative estimation of an important bio active marker Asiaticoside in Centella asiatica collected from different geographical regions of India. Three samples were collected from different geographical regions of India and analyzed by HPLC. The compound separation was achieved on C18 Eclipse, XDB, 4.6mm × 150mm, 5μm particle size column using mobile phase consisted of Acetonitrile: Buffer (25:75v/v) in isocratic elution with flow rate 1.8ml/min. The detection of analytes at 210nm was carried out by using Diode Array Detector (DAD). The comparative study was carried out for variations of Asiaticoside content in Centella Asiatica Linn Collected from different geographical regions of India i.e. Chennai (Tamilnadu), Jhansi (Uttar Pradesh) and Raniketh (Uttarakhand). The studies reveal that the maximum amount of the marker compounds Asiaticoside (0.6157%) is present in the sample collected from Chennai compare to the other regions samples.

Antibacterial Activity Test of Gotu Kola Leaf Extract (Centella asiatica) Against Staphylococcus aureus Bacteria Isolated from Wounds of Diabetes Mellitus Patients

Staphylococcus aureus, a gram-positive bacterium, is the causative agent of diabetic foot. Traditional medicine using plants needs much further research, especially for wound healing in diabetes mellitus patients. This study is an experimental laboratory investigation on the efficacy of Gotu kola (Centella asiatica) leaf extract against wound-isolated Staphylococcus aureus. Gotu kola leaf extract (12.5%, 25%, 50%, 75%, and 100%), cefixime suspension (positive control), and sterile distilled water were tested. The results of the research showed the formation of an inhibitory zone from testing Gotu kola leaf extract against Staphylococcus aureus bacteria, where the average inhibitory zone was at concentrations of 12.5% (7.2 mm), 25% (11 mm), 50% (13.7 mm), 75% (14 .3 mm), and 100% (19.5 mm). The higher the concentration used, the larger the inhibition zone formed. Research shows that Gotu kola leaves' active substances inhibit energy formation, nucleic acid formation, cell membrane permeability, structure and function, and membrane protein lysis and damage.

Harnessing postharvest light emitting diode (LED) technology of Centella asiatica (L.) Urb. to improve centelloside content by up-regulating gene expressions in the triterpenoid pathway

Centella asiatica (L.) Urb. has wound healing, anti-inflammatory, cognitive improvement, and neuroprotective properties which have been attributed to its centelloside content. However, the quantities of these bioactive compounds are limited and vary due to genetic and environmental factors. Light qualities are known to enhance the production of secondary metabolites in several plant species, both preharvest and postharvest. In this study, fresh leaves of C. asiatica were subjected to different light emitting diode (LED) quality including white, dark, red, blue, and green to assess centelloside content, phytochemical constituents, and transcription level expression of triterpenoid biosynthesis genes. Results showed that white and blue LEDs significantly increased centelloside content in C. asiatica leaves at 3 days postharvest (dph) by 73 % over the control group at 0 dph. Blue LEDs stimulated the expression of triterpenoid biosynthesis genes including C. asiatica squalene synthase (CaSQS), C. asiatica β-amyrin synthase (CabAS), and C. asiatica UDP gluclosyltransferase-73AH1 (CaUGT73AH1; CaUGT), while different LED conditions gave diverse results. Red LED treatment triggered higher total flavonoid content (TFC) and total triterpenoid content (TTC) while white LEDs enhanced total triterpenoid content (TTC). Taken together, our findings suggest that postharvest under blue LEDs is a great approach to increase centelloside production of C. asiatica through gene up-regulation in triterpenoid pathway. Therefore, postharvest technology by LEDs serves as an effective tool for improving raw material quality for medicinal plant industries.

Improvement in Yield of Extracellular Vesicles Derived from Edelweiss Callus Treated with LED Light and Enhancement of Skin Anti-Aging Indicators.

The process of skin aging is currently recognized as a disease, and extracellular vesicles (EVs) are being used to care for it. While various EVs are present in the market, there is a growing need for research on improving skin conditions through microbial and plant-derived EVs. Edelweiss is a medicinal plant and is currently an endangered species. Callus culture is a method used to protect rare medicinal plants, and recently, research on EVs using callus culture has been underway. In this study, the researchers used LED light to increase the productivity of Edelweiss EVs and confirmed that productivity was enhanced by LED exposure. Additionally, improvements in skin anti-aging indicators were observed. Notably, M-LED significantly elevated callus fresh and dry weight, with a DW/FW ratio of 4.11%, indicating enhanced proliferation. Furthermore, M-LED boosted secondary metabolite production, including a 20% increase in total flavonoids and phenolics. The study explores the influence of M-LED on EV production, revealing a 2.6-fold increase in concentration compared to darkness. This effect is consistent across different plant species (Centella asiatica, Panax ginseng), demonstrating the universality of the phenomenon. M-LED-treated EVs exhibit a concentration-dependent inhibition of reactive oxygen species (ROS) production, surpassing dark-cultured EVs. Extracellular melanin content analysis reveals M-LED-cultured EVs' efficacy in reducing melanin production. Additionally, the expression of key skin proteins (FLG, AQP3, COL1) is significantly higher in fibroblasts treated with M-LED-cultured EVs. These results are expected to provide valuable insights into research on improving the productivity of plant-derived EVs and enhancing skin treatment using plant-derived EVs.