병풀주정추출분말의 항염증 효능 검색

Abstract

Centella asiatica (C. asiatica) is currently widely used in wound healing and has recently been reported to have immune suppressive properties. This study investigated the anti-inflammatory effect with ethanol-extract of C. asiatica cultivated in a smart farm. Analysis of asiaticoside in C. asiatica extract was performed using high performance liquid chromatography (HPLC) and as a result, the asiaticoside content of C. asiatica extract was 23.17 mg/g. The effect of C. asiatica extract on the expression of inflammatory mediators, such as interleukin-1beta (IL-1β), IL-6 and tumor necrosis factor-alpha (TNF-α), and nitric oxide (NO) was examined in murine macrophage cell line, RAW264.7 using ELISA method. C. asiatica extract inhibited the production of NO, IL-1β, IL-6, and TNF-α in macrophages stimulated with lipopolysaccharide (LPS) at a dose dependent manner. C. asiatica extract also suppressed the expression of IL-6 and IL- 8 in HaCaT cells, a human keratinocytes, stimulated with TNF-α/IFN-. These results suggest that C. asiatica cultivated in a smart farm can be effectively utilized as a therapeutic agent for inflammatory disease.