Quantitative Estimation of Variability in Content of Asiaticoside in Centella asiatica Linn Collected from Different Geographical Regions of India by HPLC

Abstract

Centella asiatica Linn is a significant and highly valued medicinal plant in various indigenous system of medicine Ayurveda, Siddha and Unani Indian system of medicines and Chinese medicines. It is one of the chief herbs for treating skin disorder, wounds healing, gastrointestinal disorders, asthma, ulcers, leprosy, vein diseases and memory improvement. In the present study an attempt is made to develop HPLC method for qualitative and quantitative estimation of an important bio active marker Asiaticoside in Centella asiatica collected from different geographical regions of India. Three samples were collected from different geographical regions of India and analyzed by HPLC. The compound separation was achieved on C18 Eclipse, XDB, 4.6mm × 150mm, 5μm particle size column using mobile phase consisted of Acetonitrile: Buffer (25:75v/v) in isocratic elution with flow rate 1.8ml/min. The detection of analytes at 210nm was carried out by using Diode Array Detector (DAD). The comparative study was carried out for variations of Asiaticoside content in Centella Asiatica Linn Collected from different geographical regions of India i.e. Chennai (Tamilnadu), Jhansi (Uttar Pradesh) and Raniketh (Uttarakhand). The studies reveal that the maximum amount of the marker compounds Asiaticoside (0.6157%) is present in the sample collected from Chennai compare to the other regions samples.