Cell Retention Time Research Articles

Nitrite-Oxidizing Bacterial Strains Isolated from Soils of Andean Ecosystems and Their Potential Use in Nitrogen Reduction

Nitrification is part of the nitrogen cycle that occurs naturally in ecosystems. It is related to the presence of microorganisms and their metabolism, especially bacteria, which are involved in oxidizing compounds such as NH4+ and NO2− to NO3−. In this study, we evaluated the nitrification potential in 12 bacteria strains that belong to the genera Aeromonas, Bacillus, Buttiauxella, Mycobacterium, Paenibacillus, Serratia, and Yersenia, which are part of the cultivable microbial community from soil in a native forest and pine forest in The Labrado area within the Machangara micro-watershed in the Andes located in the south of Ecuador. This investigation aims to identify heterotrophic and lithoautotrophic strains using specific culture media for ammonium oxidative (AOL-AOH) and nitrate oxidation bacteria (ONL-ONH). The formation of nitrifying halos in the culture media allowed the identification of 10 strains with nitrifying potential. Five strains were from the pine forest, four were isolated from the native forest, and one strain was shared between both forests. The Serratia and Yersinia genera have a high NO2− oxidation capacity. Their inoculation in synthetic water rich in nitrogenous products allowed us to determine 40% and 94% nitrite reduction percentages and cell retention times of 20 to 40 days. Our results are promising for their possible potential use in environmental bioremediation processes through inoculation in wastewater for the biological removal of nitrogenous compounds.

Transplantation of Wharton's jelly mesenchymal stem cells encapsulated with Hydroactive® Gel promotes diabetic wound antifibrotic healing in type 2 diabetic rats.

Diabetic cutaneous ulcers (DCU) are a complication for diabetes patients, mostly occurring in the foot and causing non-healing diabetic foot ulcers. Mesenchymal stem cell (MSC)-based therapy is currently being investigated as a therapeutic avenue for chronic diabetic ulcers. However, poor engraftment, short retention, and low survival still limit the treatment effectiveness. Hydroactive® Gel is a sterile transparent gel made of natural hydrocolloid, which has been widely used for wound management. Whether transplantation of Wharton's jelly mesenchymal stem cells (WJMSCs) encapsulated with Hydroactive® Gel is helpful to diabetic ulcers wound healing remains to be explored. The biocompatibility experiments showed that WJMSCs embedded in Hydroactive® Gel did not influence the cell viability, survival, proliferation, and apoptosis of WJMSCs in vitro. RNA-seq results also implied that Hydroactive® Gel + WJMSCs transplantation activated the "cytokine-cytokine receptor interaction", "mononuclear cell differentiation", "regulation of cell-cell adhesion", and "chemokine receptor activity" to accelerate the inflammatory reaction and epidermis regeneration in diabetic wounds. Histological analysis results demonstrated that Hydroactive® Gel encapsulated WJMSCs transplantation promoted diabetic wound healing and regeneration, indicating improved dermis regeneration, sebaceous gland formation, and type III collagen fiber deposition. Besides, immunohistochemical analysis results showed that Hydroactive® Gel + WJMSCs transplantation also facilitated the transformation of pro-inflammatory M1 macrophages to anti-inflammatory M2 macrophages, cell proliferation, and neovascularization at the wound site. Hydroactive® Gel encapsulation further prolonged the retention time of WJMSCs at the diabetic wound site. Above all, Hydroactive® Gel accelerates WJMSCs-mediated diabetic wound healing by promoting macrophage transformation, facilitating cell proliferation and angiogenesis, and prolonging cell retention time. Our findings may potentially provide a useful therapeutic strategy based on the combination of WJMSCs and biomedical materials for patients with diabetic cutaneous ulcers.

Optimum operational conditions for mixotrophic microalgae growth and nutrient recovery

Microalgae have been presented as microorganisms with great potential to recover nutrient from wastewater. Mixotrophic cultivation of microalgae in nutrient rich wastewater can help eliminating the deficiencies of both phototrophic and heterotrophic growth by allowing the independent optimisation of respiration and photosynthesis processes. Nutrient control and uptake by mixotrophic microalgae can be achieved either in a single or two-stage process using sequential reactors in a continuous flow system. Therefore, this work aims at studying mixotrophic microalgae growth in a two-stage biological process under continuous flow conditions with biomass recycle to recover nutrients from wastewater, considering the effects of different operational conditions (hydraulic retention time (HRT), cell retention time (CRT) and different nitrogen sources). The optimum operational conditions for algal nutrient uptake were identified to be 48 h HRT and 14 d CRT, using a mix of nitrogen sources (Ammonium-N to Nitrate-N ratio of 1:1) with 40.0% and 93.2% of phosphorus of nitrogen recovery in algal biomass, respectively.

Design and Encapsulation of Immunomodulators onto Gold Nanoparticles in Cancer Immunotherapy.

The aim of cancer immunotherapy is to reactivate autoimmune responses to combat cancer cells. To stimulate the immune system, immunomodulators, such as adjuvants, cytokines, vaccines, and checkpoint inhibitors, are extensively designed and studied. Immunomodulators have several drawbacks, such as drug instability, limited half-life, rapid drug clearance, and uncontrolled immune responses when used directly in cancer immunotherapy. Several strategies have been used to overcome these limitations. A simple and effective approach is the loading of immunomodulators onto gold-based nanoparticles (GNPs). As gold is highly biocompatible, GNPs can be administered intravenously, which aids in increasing cancer cell permeability and retention time. Various gold nanoplatforms, including nanospheres, nanoshells, nanorods, nanocages, and nanostars have been effectively used in cancer immunotherapy. Gold nanostars (GNS) are one of the most promising GNP platforms because of their unusual star-shaped geometry, which significantly increases light absorption and provides high photon-to-heat conversion efficiency due to the plasmonic effect. As a result, GNPs are a useful vehicle for delivering antigens and adjuvants that support the immune system in killing tumor cells by facilitating or activating cytotoxic T lymphocytes. This review represents recent progress in encapsulating immunomodulators into GNPs for utility in a cancer immunotherapeutic regimen.

Advanced hybrid electro-oxidation & O3 technology for water reuse in the fruit and vegetable process industry.

Alternative sanitising agents are required in the fruit and vegetable processing industry, capable of effectively disinfecting both the product and the washing water to increase its reuse, while not producing harmful by-products like chlorine-based agents. In the present work, electro-oxidation (EO) and ozone are proposed for vegetable process water reuse, being optimised for their individual and combined application. The application of hybrid electro-oxidation and ozone achieved 7 log reduction after 15 min of treatment in batch using boron-doped diamond (BDD) as anode material, showing important synergistic effects when compared with the individual treatments. When the process was applied in continuous mode, up to 6 log reduction of total bacteria was achieved using EO alone with Ru as the anode and stainless steel as the cathode, under 11 mA/cm2 of current density, a cell retention time of 12 s and no addition of chemical reagents (electrolyte). Under these conditions, the absence of microorganisms in water was maintained for more than 24 h, no harmful by-products (chlorate, bromate) were detected and no damage to lettuce seeds were observed when evaluating water reuse potential.

AFM26 - Targeting B Cell Maturation Antigen (BCMA) for NK Cell-Mediated Immunotherapy of Multiple Myeloma

Significant progress in multiple myeloma (MM) treatment has been made in recent years and achieving sustained minimal residual disease (MRD) negativity now constitutes a major goal of treatment. High dose melphalan combined with autologous stem cell transplantation (ASCT) is currently regarded the standard of care in 1st line treatment of MM in eligible patients, but fails to eradicate MRD in the majority of patients ultimately leading to disease relapse. Consequently, additional therapies are needed to target residual disease to delay or potentially prevent relapse.The cell surface antigen B cell maturation antigen (BCMA, CD269) constitutes a highly attractive target antigen for immunotherapy because of its restricted expression in non-malignant tissue but almost universal expression on MM cells.AFM26 is a BCMA-targeted tetravalent bispecific antibody that selectively engages natural killer (NK) cells to induce myeloma cell lysis. Through its high-affinity bivalent binding to CD16A (FcγRIIIa), AFM26 possesses prolonged cell retention times, and has been shown to be less prone to interference by high levels of circulating IgG than antibody Fc-domains. Thus, AFM26 can be used to “arm” NK cells for effective killing of tumor cells. AFM26 also induces markedly lower levels of pro-inflammatory cytokines in peripheral blood mononuclear cell (PBMC) cultures in the presence of target cells compared to BCMA-directed T cell activating approaches. In contrast to other monoclonal antibodies (mAbs) such as daratumumab and elotuzumab, AFM26 does not confer target-independent NK cell activation or NK cell depletion.NK cells are promising effectors to target MRD. Due to their early reconstitution following ASCT and their ability to rapidly destroy malignant cells through direct cytotoxicity. Redirecting NK cells through AFM26 may therefore offer an effective treatment of MM patients early after ASCT.Employing a wide range of functional assays, we demonstrate here that AFM26 exhibits potent cytotoxic activity against primary myeloma cells using patient (autologous) and donor-derived (allogeneic) NK cells as well as against a panel of high and low BCMA expressing cell lines. In particular, we show that AFM26 induces NK cell cytotoxicity towards cells expressing almost undetectable surface levels of BCMA, suggesting the potential for broad and deep anti-MM activity. In summary, AFM26 is well-differentiated from other mAbs and a promising therapeutic candidate to address the unmet medical need of MRD positivity in MM. DisclosuresGantke:Affimed: Employment. Reusch:Affimed: Employment. Kellner:Affimed: Research Funding. Klausz:Affimed: Research Funding. Haneke:Affimed: Employment. Knackmuss:Affimed: Employment. Müller:Affimed: Employment. Ellwanger:Affimed: Employment. Fucek:Affimed: Employment. Schniegler-Mattox:Affimed: Employment. Koch:Affimed: Employment. Kerber:Affimed: Employment. Peipp:Affimed: Research Funding. Treder:Affimed: Employment.

A Logic-Compatible eDRAM Compute-In-Memory With Embedded ADCs for Processing Neural Networks

A novel 4T2C ternary embedded DRAM (eDRAM) cell is proposed for computing a vector-matrix multiplication in the memory array. The proposed eDRAM-based compute-in-memory (CIM) architecture addresses a well-known Von Neumann bottle-neck in the traditional computer architecture and improves both latency and energy in processing neural networks. The proposed ternary eDRAM cell takes a smaller area than prior SRAM-based bitcells using 6-12 transistors. Nevertheless, the compact eDRAM cell stores a ternary state (-1, 0, or +1), while the SRAM bitcells can only store a binary state. We also present a method to mitigate the compute accuracy degradation issue due to device mismatches and variations. Besides, we extend the eDRAM cell retention time to 200μs by adding a custom metal capacitor at the storage node. With the improved retention time, the overall energy consumption of eDRAM macro, including a regular refresh operation, is lower than most of prior SRAM-based CIM macros. A 128×128 ternary eDRAM macro computes a vector-matrix multiplication between a vector with 64 binary inputs and a matrix with 64 × 128 ternary weights. Hence, 128 outputs are generated in parallel. Note that both weight and input bit-precisions are programmable for supporting a wide range of edge computing applications with different performance requirements. The bit-precisions are readily tunable by assigning a variable number of eDRAM cells per weight or adding multiple pulses to input. An embedded column ADC based on replica cells sweeps the reference level for 2 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">N</sup> -1 cycles and converts the analog accumulated bitline voltage to a 1-5bit digital output. A critical bitline accumulate operation is simulated (Monte-Carlo, 3K runs). It shows the standard deviation of 2.84% that could degrade the classification accuracy of the MNIST dataset by 0.6% and the CIFAR-10 dataset by 1.3% versus a baseline with no variation. The simulated energy is 1.81fJ/operation, and the energy efficiency is 552.5-17.8TOPS/W (for 1-5bit ADC) at 200MHz using 65nm technology.

Dynamic simulation of continuous mixed sugar fermentation with increasing cell retention time for lactic acid production using Enterococcus mundtii QU 25

BackgroundThe simultaneous and effective conversion of both pentose and hexose in fermentation is a critical and challenging task toward the lignocellulosic economy. This study aims to investigate the feasibility of an innovative co-fermentation process featuring with a cell recycling unit (CF/CR) for mixed sugar utilization. A l-lactic acid-producing strain Enterococcus mundtii QU 25 was applied in the continuous fermentation process, and the mixed sugars were utilized at different productivities after the flowing conditions were changed. A mathematical model was constructed with the experiments to optimize the biological process and clarify the cell metabolism through kinetics analysis. The structured model, kinetic parameters, and achievement of the fermentation strategy shall provide new insights toward whole sugar fermentation via real-time monitoring for process control and optimization.ResultsSignificant carbon catabolite repression in co-fermentation using a glucose/xylose mixture was overcome by replacing glucose with cellobiose, and the ratio of consumed pentose to consumed hexose increased significantly from 0.096 to 0.461 by mass. An outstanding product concentration of 65.2 g L−1 and productivity of 13.03 g L−1 h−1 were achieved with 50 g L−1 cellobiose and 30 g L−1 xylose at an optimized dilution rate of 0.2 h−1, and the cell retention time gradually increased. Among the total lactic acid production, xylose contributed to more than 34% of the mixed sugars, which was close to the related contents in agricultural residuals. The model successfully simulated the transition of sugar consumption, cell growth, and lactic acid production among the batch, continuous process, and CF/CR systems.ConclusionCell retention time played a critical role in balancing pentose and hexose consumption, cell decay, and lactic acid production in the CF/CR process. With increasing cell concentration, consumption of mixed sugars increased with the productivity of the final product; hence, the impact of substrate inhibition was reduced. With the validated parameters, the model showed the highest accuracy simulating the CF/CR process, and significantly longer cell retention times compared to hydraulic retention time were tested.

Effects of encapsulation on the chemical inhibition of anaerobic hydrogen- and methane-producing microbial cells

Encapsulating microbial cells in an alginate matrix enables high-rate anaerobic biological treatment and resource recovery by increasing cell concentration and retention time. To determine whether encapsulation protected microbial cells from inhibitors, copper(II), chromium(VI) (as dichromate), ammonium, and chloroform were amended to hydrogen-producing anaerobic microbial cells that were either suspended or encapsulated in a composite-coated alginate matrix. Encapsulation mitigated dichromate inhibition by 36% based on the dose-response curve slope but increased ammonium inhibition by 210% and had no effect on inhibition by chloroform. This was explained by effective concentrations in the beads after measuring partition coefficients. Encapsulation rendered copper(II) unavailable through apparent chelation because it protected both the hydrogen-producing and a methane-producing community from copper(II) despite its high effective concentration in encapsulation. Understanding the effects that the encapsulation of microbial cells has on chemical inhibition can help expand the application conditions of wastewater treatment or resource recovery and avoid unanticipated toxicity.

Reducing DRAM refresh power consumption by runtime profiling of retention time and dual-row activation

Refresh power of dynamic random-access memory (DRAM) has become a critical problem due to the large memory capacity of servers and mobile devices. It is imperative to reduce refresh rate in order to reduce refresh power consumption. However, current methods of refresh rate improvement have limitations such as large area/performance overhead, low system availability, and lack of support at the memory controller. We propose a novel scheme which comprises three essential functions: (1) an adaptive refresh method that adjusts refresh period on each DRAM chip, (2) a runtime method of retention-time profiling that operates inside DRAM chips during idle time thereby improving availability, and (3) a dual-row activation method which improves weak cell retention time at a very small area cost. The proposed scheme allows each DRAM chip to refresh with its own refresh period without requiring the external support. Experiments based on real DRAM chip measurements show that the proposed methods can increase refresh period by 4.5 times at 58 °C by adjusting refresh period in a temperature-aware manner while incurring only a small overhead of 1.05% and 0.02% in DRAM chip area and power consumption, respectively. Below 58 °C, our method improves the refresh period by 12.5% compared with two state-of-the-art methods, AVATAR and in-DRAM ECC. In various memory configurations with SPEC benchmarks, our method outperforms the existing ones in terms of energy-delay product by 19.7% compared with the baseline, and by 15.4% and 12.4%, with respect to AVATAR and in-DRAM ECC, respectively.

Plant-Based Calcium Fructoborate as Boron-Carrying Nanoparticles for Neutron Cancer Therapy

Boron neutron capture therapy (BNCT) is an effective clinical method in cancer treatment based on fission reactions and nuclear capturing. In this method, use of the best boron-containing agents for boron therapy and boron delivery agent for transfer to the infectious site are the key points for efficienct treatment. Our research indicated that calcium fructoborate(CF) was the best compound as a boron-containing agent for boron therapy. Furthermore, studies have demonstrated that liposomes can selectively and effectively deliver large quantities of boron to cells and that the compounds delivered by liposomes have a longer cell retention time. Indeed, liposomal encapsulation technology of CF as nanostructured liposome carriers (NLCs) was extensively evaluated due to the ability of these nano-vehicles for the delivery of boron compounds. In this work, the molecular composition of the CF used as a carrier supplement for cancer therapy is deeply investigated. FTIR, XRD, TG, DSC and Raman spectroscopic analyses were used for the characterization of the carrier. The experimental measurements agreed very well with the molecular formula of Ca[(C6H10O6)2B]24H2O.

Expanded granular sludge bed bioreactor in wastewater treatment

The expanded granular sludge bed bioreactor appears today as a cheap, robust and more popular technology because it operates using a fluidized bed, which allows increasing in organic load and in cell retention times, generating higher efficiencies (up to 95 %) and renewable energy (i.e., biogas, biomethane, and biohydrogen). Nevertheless, the efficiency of this bioreactor mainly depends on the operating conditions. Thus, the content presented in this review paper focuses on the analysis of the operating conditions and performance of expanded granular sludge bed bioreactor for treating different types of industrial, agro-industrial and domestic wastewaters (e.g., agro-food, beverage, alcohol distillery, tannery, slaughterhouse, chemical, pharmaceutical, municipal sewage, among others). Because of this reason, this study aimed to analyze the operating conditions and type of substrate, which has been used in these bioreactors to improve future research to wastewater and renewable energy production. According to the review, it is concluded that the EGSB bioreactor is a novel sustainable alternative to treat different types of wastewaters and consequently change the paradigm of wastewater management from treatment and disposal to beneficial use as well as profitable effort.

On the Low-Frequency Noise Characterization of Z2-FET Devices

This paper addresses the low-frequency noise characterization of Z 2 -FET structures. These double-gated p-i-n diode devices have been fabricated at STMicroelectronics in an ultrathin body and box (UTBB) 28-nm FDSOI technology and designed to operate as 1T-DRAM memory cells, although other applications, as for example electro static discharge (ESD) protection, have been reported. The experimentally extracted power spectral density of current reveals that the high-diode series resistance, carrier number fluctuations due to oxide traps, and gate leakage current are the main noise contributors at high-current regimes. These mechanisms are expected to contribute to the degradation of cell variability and retention time. Higher flicker noise levels have been reported when increasing the vertical electric field. A simple model considering the contribution of the main noise sources is proposed.

Performance and bacterial diversity of bioreactors used for simultaneous removal of sulfide, solids and organic matter from UASB reactor effluents.

Two bioreactors were investigated as an alternative to post-treatment of effluent from an upflow anaerobic sludge blanket (UASB) reactor treating domestic sewage, with an aim of oxidizing sulfide into elemental sulfur, and removal of solid and organic material. The bioreactors were operated at different hydraulic retention times (HRTs) (6, 4, and 2 h) and in the presence or absence (control) of packing material (polypropylene rings). Greater sulfide removal efficiencies - 75% (control reactor) and 92% (packed reactor) - were achieved in both reactors for an HRT of 6 h. Higher organic matter (COD) and solid (TSS) removal levels were observed in the packed reactor, which produced effluent with low COD (100 mg CODL-1) and TSS concentrations (30 mg TSSL-1). Denaturing gradient gel electrophoresis results revealed that a metabolically diverse bacterial community was present in both bioreactors, with sequences related to heterotrophic bacteria, sulfur bacteria (Thiocapsa, Sulfurimonas sp., Chlorobaculum sp., Chromatiales and Sulfuricellales), phototrophic purple non-sulfur bacteria (Rhodopseudomonas, Rhodocyclus sp.) and cyanobacteria. The packed reactor presented higher extracellular sulfur formation and potential for elemental sulfur recovery was seen. Higher efficiencies related to the packed reactor were attributed to the presence of packing material and higher cell retention time. The studied bioreactors seemed to be a simple and low-cost alternative for the post-treatment of anaerobic effluent.

TWiCe: Time Window Counter Based Row Refresh to Prevent Row-Hammering

Computer systems using DRAM are exposed to row-hammering attacks, which can flip data in a DRAM row without directly accessing a row but by frequently activating its adjacent ones. There have been a number of proposals to prevent row-hammering, but they either incur large area/performance overhead or provide probabilistic protection. In this paper, we propose a new row-hammering mitigation mechanism named T ime Wi ndow C ount e r based row refresh (TWiCe) which prevents row-hammering by using a small number of counters without performance overhead. We first make a key observation that the number of rows that can cause flipping their adjacent ones (aggressor candidates) is limited by the maximum values of row activation frequency and DRAM cell retention time. TWiCe exploits this limit to reduce the required number of counter entries by counting only actually activated DRAM rows and periodically invalidating the entries that are not activated frequently enough to be an aggressor. We calculate the maximum number of required counter entries per DRAM bank, with which row-hammering prevention is guaranteed. We further improve energy efficiency by adopting a pseudo-associative cache design to TWiCe. Our analysis shows that TWiCe incurs no performance overhead on normal DRAM operations and less than 0.7 percent area and energy overheads over contemporary DRAM devices.

DARE

Power consumption and reliability of memory components are two of the most important hurdles in realizing exascale systems. Dynamic random access memory (DRAM) scaling projections predict significant performance and power penalty due to the conventional use of pessimistic refresh periods catering for worst-case cell retention times. Recent approaches relax those pessimistic refresh rates only on ``strong'' cells, or build on application-specific error resilience for data placement. However, these approaches cannot reveal the full potential of a relaxed refresh paradigm shift, since they neglect additional application resilience properties related to the inherent functioning of DRAM. In this article, we elevate Refresh-by-Access as a first-class property of application resilience. We develop a complete, non-intrusive system stack, armed with low-cost Data-Access Aware Refresh (DARE) methods, to facilitate aggressive refresh relaxation and ensure non-disruptive operation on commodity servers. Essentially, our proposed access-aware scheduling of application tasks intelligently amplifies the impact of the implicit refresh of memory accesses, extending the period during which hardware refresh remains disabled, while limiting the number of potential errors, hence their impact on an application's output quality. The stack, implemented on an off-the-shelf server and running a full-fledged Linux OS, captures for the first time the intricate time-dependent system and data interactions in the presence of hardware errors, in contrast to previous architectural simulations approaches of limited detail. Results demonstrate that by applying DARE, it is possible to completely disable hardware refresh, with minor quality loss that ranges from 2% to 18%.

Hydrodynamic Performance of an Expanded Granular Sludge Bed Reactor for the Removal of Organic Matter

Expanded granular sludge bed (EGSB) reactors are classified as third-generation anaerobic systems. They operate using a fluidized bed, which allows an increase in organic load, a decrease in hydraulic retention times, an increase in cell retention times, and higher treatment efficiencies compared with upflow anaerobic sludge blanket reactors. In this research, two EGSB reactors were employed and the upflow velocity, temperature, and organic load were varied. Reactors were operated with synthetic wastewater using a biodegradable substrate (dextrose). The residence time distribution of the reactor was analyzed using tracer tests. A temperature of 35°C influenced bacterial degradation kinetics, allowing an increase in the percentage of organic matter removal. At ambient temperature, a removal of 89.49% was obtained with an organic loading rate (OLR) of 3.39 kg/m3·day, while at 35°C the best removal was 93.98% with a higher OLR (4.23 kg/m3·day). Recirculation in reactors improved fluidization of the bed and mixing, favoring granulation of biomass and allowing operation of reactors under a fully mixed tank model. Biomass granulation improved settleability and made the process more efficient. It was found that an upflow velocity of 6 m/h at ambient temperature produced a high removal of chemical oxygen demand (96.83%).

A red-emissive antibody-AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells.

An antibody-AIEgen conjugate is designed and developed as a "turn-on" fluorescent probe for wash-free specific cancer cell imaging. The cetuximab-conjugated AIEgen shows red fluorescence only when it is internalized and accumulated in cancer cells with overexpressed epidermal growth factor receptor through endocytosis. The probe first lights up the lysosomes. After hydrolysis, its residue is accumulated in mitochondria, making them highly emissive with a long cell retention time. Compared with conventional "always-on" fluorescent probes, the antibody-AIEgen conjugate exhibits a very good image contrast during wash-free cancer cell imaging and less interference from normal cells. To the best of our knowledge, this is the first time "turn-on" antibody-AIEgen conjugates have been reported. This new strategy can be further extended to many proteins and water-soluble AIEgens, and many of their potential applications such as real-time tracking of cell dynamics and cancer theranostics will be explored. The present work is expected to inspire more marvellous research in the fields of AIE and cancer imaging.

Kinetics of bisphenol A degradation by Sphingomonas paucimobilis FJ-4

A chemostat was operated to characterize degradation of bisphenol A by Sphingomonas paucimobilis FJ-4. The chemostat at 30°C was fed with a medium containing 150mgL(-1) of BPA as the sole carbon and energy source. At the short cell retention time of 8h, the bacterial cells were washed out from the chemostat. At long cell retention times of 12, 16, 24, and 48h, steady-states of the bacterial growth on BPA degradation were achieved after a lag time of 16-57h. A mathematical model was applied to evaluate the BPA degradation ability of strain FJ-4. The maximum specific degradation rate, the half saturation constant, the cell yield, and the specific decay rate were estimated respectively as 0.46mg-BPA (mg-VSS h)(-1), 13.1mgL(-1), 0.39mg-VSS mg-BPA(-1), and 0.0014h(-1).

Trade-off between carbon emission and effluent quality of activated sludge processes under seasonal variations of wastewater temperature and mean cell retention time

Over the seasonal cycles, the mean cell retention time (MCRT) of the activated sludge process is varied to compensate the wastewater temperature variations. The effects of these variations on the carbon footprint (CFP) and effluent quality index (EQI) of a conventional activated sludge (CAS) process and a nitrification/denitrification (NDN) process were quantified. The carbon emission included both biogenic and non-biogenic carbon. Carbon emissions of wasted biosolids management were also addressed. Our results confirmed that the effluent quality indicated by EQI was not necessarily improved by increasing MCRT. Higher MCRT increased the carbon emission and reduced excess sludge production, which decreased the potential for biogas energy recovery. The NDN process was preferable to the CAS process from the perspective of effluent quality. This consideration extended to the whole plant CFP if the N2O emitted during NDN was limited ([N2O]<1% [NH4+]removed) as the carbon emission per unit effluent quality achieved by NDN process is less than that of the CAS process. By putting forward carbon emission intensity (γ) derived from CFP and EQI, our work provides a quantitative tool for decision makers evaluating process alternatives when there is a trade-off between carbon emission and effluent quality.