CAST Gene Research Articles

ГЕНЕТИЧЕСКАЯ СТРУКТУРА ОВЕЦ ЗАПАДНО-СИБИРСКОЙ МЯСНОЙ И КУЛУНДИНСКОЙ ТОНКОРУННОЙ ПОРОД ПО ГЕНАМ CAST, GDF9 И KRT1.2

The purpose of the study is to analyze the genotypic structure and population-genetic parameters of the West Siberian meat and Kulunda fine-wool breed of sheep by the CAST, GDF9 and KRT1.2 genes and blood groups. Molecularnogenetic and immunogenetic studies were carried out in the SRIAH (Siberian Research Institute of Animal Husbandry SFSCA RAS) biotechnology laboratory. PCR-RFLP analysis to determine genotypes was performed according to the described and approved methods on a C1000 “BioRad” amplifier; the results were visualized using an E-Box-CX5.TS-20.M gel documentation system. It was found that the frequency of genotypes and alleles of the CAST gene of West Siberian meat and Kulunda fine-wool breeds have no significant differences. In both breeds, CASTMM was predominant, with a frequency of 69.0–75.0%, CASTNN carriers being only 1.6–5.1%. The Kulunda fine-wool breed is characterized by a very high frequency of the GDF9GG genotype 92.7%, which is higher than that of the West Siberian meat sheep by 26.3%. GDF9AA genotype is extremely rare in breeds (0–5%). The GDF9G allele in the compared breeds was found to range from 0.811–0.960 and the GDF9A allele from 0.189–0.040. There are differences in the ratio of genotypes of the KRT1.2 gene in the compared breeds. In the Kulunda fine-wool breed, the vast majority of animals have homozygous genotype KRT1.2MM (95.5%), whereas in the West Siberian meat breed such sheep constitute 32.8%. Consequently, the frequency of the KRT1.2M allele is 0.565–0.978 and the KRT1.2N allele is 0.435–0.022. The genetic balance in the studied genes is not disturbed, χ2 = 0,033–1,025. The index of genetic similarity between breeds, calculated on the basis of the frequencies of genotypes and blood groups is 0.901 ± 0.028 and 0.833 ± 0.024. Population and genetic characteristics are practically identical in the compared breeds, except for gene homozygosity (Ca), which is higher in the Kulunda fine-wool sheep breed by 24.3% for the GDF9 gene and KRT1.2 by 29.1%, compared to the West Siberian meat sheep breed. The number of effective alleles is insignificant (1.04–1.46). The genetic variability (V) by individual genes in the breeds varies from 5.0 to 49.6%. The negative value of the Fis coefficient indicates the absence of inbreeding in sheep the studied breeds.

Genetic variation of CAST gene in Local Karnobat and Karnobat merino sheep breeds

Karnobat sheep plays an important role in the development of sheep breeding in Southeastern region of Bulgaria. They are valuable source of genetic material. The aim of present experiment was to determine the allele variation of CAST gene in Local Karnobat and Karnobat Merino sheep breeds. A total of 60 blood samples were collected – 30 per breed. DNA was extracted and genotypes of all animals were identified by means of PCR-RFLP technique. The restriction reactions were accomplished by specific enzymeMspI. As expected both breeds were characterized with low level of genetic diversity due to the fact that mostly maintaining selection has been implemented. In Local Karnobat sheep breed was identified only one heterozygous individual from all 30. In Karnobat merino were identified allele M with frequency 0,97 and allele N with frequency 0,03. Genotypes MM and MN were revealed with frequencies 0,93 and 0,07, respectively. According to the statistical analysis both breeds were in HWE equilibrium.

Genes and proteins associated with ribeye area and meat tenderness in a commercial Nellore cattle population.

Despite several studies on genetic markers and differentially expressed genes related to ribeye area (REA) and tenderness traits in beef cattle, there is divergence in the results regarding the genes associated with these traits. Thirteen genes associated with or exhibiting biological functions that might influence such phenotypes were included in this study. A total of five genes for REA (IGF-1, IGF-2, MSTN, NEDD4, and UBE4A) and eight genes for meat tenderness (CAPN1, CAPN2, CAST, HSPB1, DNAJA1, FABP4, SCD, and PRKAG3) were selected from previous studies on beef cattle. Genes and their respective proteins expression were validated in a commercial population of Nellore cattle using quantitative real-time PCR (RT-qPCR) and advanced mass spectrometry (LC/MS-MS) techniques, respectively. The MSTN gene was upregulated in animals with low REA. The CAPN1, CAPN2, CAST, HSPB1, and DNAJA1 genes were upregulated in animals with tough meat. The proteins translated by these genes were not differentially expressed. Our results confirm the potential of some of the studied genes as biomarkers for carcass and meat quality traits in Nellore cattle.

Genome-wide CNV analysis reveals variants associated with high-altitude adaptation and meat traits in Qaidam cattle

Qaidam cattle are local breeds that habitats in northwest China. It has many excellent characteristics, such as high cold and roughage tolerance, low oxygen adaptability, and tender meat quality. Copy number variation (CNV) can induce phenotypic changes in animals by a variety of effects, and thus affects the biological functions of the animals. To explore the molecular mechanism of its adaptation to extreme cold weather and muscle fat development, the CNV variations in the genome of three Qaidam cattle were detected by whole-genome sequencing, in this study. A total of 16,743 CNVs and 9498 copy number variable regions (CNVRs) were obtained after the screening, which accounts for 2.18% of the bovine genome. The CNVR length detected ranged from 0.3 KB to 10.77 KB, with a total length of 58.17 MB and an average length of 6.12 KB/ CNVR. Through functional enrichment of CNVR related genes, LDHB, and ME1 genes were screened as the key genes for Qaidam cattle to adapt to the cold and low oxygen environments, whereas KIT and FGF18 genes might be related to the coat color and growth. In the CNVR overlapped with QTLs, variation in CAPN1 and CAST genes might be closely related to the tender meat quality of Qaidam cattle. Therefore, this study provides new genetic insights on the environmental adaptability and important economic traits of Qaidam cattle. How to cite: Guo S, Wu X, Pei J, et al. Genome-wide CNV analysis reveals variants associated with high-altitude adaptation and meat traits in Qaidam cattle. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.07.006

PSVI-21 Fatty acid composition of blood lipids in lambs of different genotypes in ontogeny

Abstract The early period of postnatal ontogeny of sheep is characterized by a variety of metabolic processes that result in the formation and consolidation of a certain type of metabolism. A special role is played by lipids, which together with proteins and carbohydrates constitute the bulk of organic compounds, cells and the organism as a whole. The purpose of this research was to study the polymorphism of CAST and GH genes as well as its association with the fatty acid composition of blood plasma lipids in lambs. The content of fatty acids was determined by gas-liquid chromatography in the form of methyl esters. From the data obtained, it follows that in all studied ontogeny periods the peripheral blood of sheep with CASTNN and CASTMM genotypes had a higher concentration of fatty acids such as palmitic, stearic, linoleic, and arachidonic acids. The content is as follows: 27.58, 45.40, 3.16, 0.28% vs. 26.21, 45.31, 3.62, 0.18% at the age of 2 months; 25.07, 43.25, 6.05, 1.74% vs. 23.24, 38.76, 6.49, 1.32% at the age of 4 months; 20.64, 22.26, 14.17, 3.38% vs. 17.64, 19.91, 15.07, 2.46% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Studies of blood lipid composition of GHBBand GHAA genotype carriers showed that palmitic, stearic, linoleic, and arachidonic acids were dominant: 27.95, 27.96, 19.04% vs. 26.08, 23.88, 17.56% at the age of 2 months, 46.87, 39.87, 22.37% vs. 3.24, 6.87, 15.56% at the age of 4 months; 0.45, 1.68, 3.81% vs. 0.29, 1.29, 3.19% at the age of 8 months (P ˂ 0.05), (P ˂ 0.01). Thus, this study emphasizes that there is a close relationship between the ratio of fatty acids in total blood lipids in lambs and the intensity of growth and development, ensuring and controlling the direction of biochemical processes in the growth and development of young sheep.

Genetic structure of the herd by genes GDF9, GH, CAST in merino sheep of the North Caucasus region of Russia

One of the main tasks in sheep breeding is to increase productivity. The use of DNA markers for the selection of animals containing the desired alleles of genes of economically valuable traits is one of the new scientific approaches to solving this problem. The aim of the work was to study the genetic structure of sheep of various breeds by genes GDF9, GH, CAST. The studies were carried out on the basis of the laboratory of molecular diagnostics and biotechnology of farm animals of the Don State Agrarian University and the Center for the collective use of scientific equipment “Bioresources and bioengineering of farm animals” of the All-Russian Scientific Research Institute of Animal Husbandry named after academician L.K. Ernst. To carry out molecular genetic studies, samples of ear pits (tissue from the auricle) with an area of 1 cm2 were taken from sheep. The evaluation was carried out by PCR-RFLP method. As a result of DNA genotyping for genes GDF9, GH, CAST, the genetic structure of sheep of such breeds as: Salskaya, Stavropol, Volgograd and Soviet merino was revealed. A and B allelic variants of the GDF9 gene, alleles A and B of the GH gene, alleles A and B of the CAST gene were identified in all studied groups of sheep. Analysis of the data revealed the breed aspect in the distribution of the allele and genotype frequencies of the GDF9, GH, CAST genes in the populations under consideration.

Determination of CAST gene polymorphism in sheep of the Volgograd breed

The scientific publication presents the results of studies of CAST polymorphism in sheep of the Volgograd breed. Against the background of the limited application of the results of genetic studies when working with the Volgograd breed, the search for polymorphic variants of the calpastatin gene, which is a potential marker of meat quality during maturation after slaughter, and also, according to some data, the live weight of sheep, is an urgent task. Calpastatin is an enzyme belonging to the calpain family of enzymes. CAST gene polymorphism was determined by PCR-RFLP analysis. To carry out molecular genetic studies in sheep of the Volgograd breed (n = 131), tissue samples were taken from the auricle with an area of 1 cm2 (ear pinches). In sheep of the Volgograd breed, the presence of two genotypes MM, MN was established with a frequency of 88 and 12%, respectively. The M allele and the homozygous MM genotype had the highest frequency. The analysis of the materials obtained as a result of the control slaughter shows that the Volgograd sheep of the MN/CAST genotype exceeded their peers with the MM/CAST genotypes by 3.7 kg in pre-slaughter weight. The presence of variability at these loci provides a basis for controlling meat productivity in sheep of the Volgograd sheep population, therefore, in breeding farms, monitoring of polymorphism and selection for the calpastatin gene should be carried out.

Expression quantitative trait loci in sheep liver and muscle contribute to variations in meat traits

BackgroundVariants that regulate transcription, such as expression quantitative trait loci (eQTL), have shown enrichment in genome-wide association studies (GWAS) for mammalian complex traits. However, no study has reported eQTL in sheep, although it is an important agricultural species for which many GWAS of complex meat traits have been conducted. Using RNA sequence data produced from liver and muscle from 149 sheep and imputed whole-genome single nucleotide polymorphisms (SNPs), our aim was to dissect the genetic architecture of the transcriptome by associating sheep genotypes with three major molecular phenotypes including gene expression (geQTL), exon expression (eeQTL) and RNA splicing (sQTL). We also examined these three types of eQTL for their enrichment in GWAS of multi-meat traits and fatty acid profiles.ResultsWhereas a relatively small number of molecular phenotypes were significantly heritable (h2 > 0, P < 0.05), their mean heritability ranged from 0.67 to 0.73 for liver and from 0.71 to 0.77 for muscle. Association analysis between molecular phenotypes and SNPs within ± 1 Mb identified many significant cis-eQTL (false discovery rate, FDR < 0.01). The median distance between the eQTL and transcription start sites (TSS) ranged from 68 to 153 kb across the three eQTL types. The number of common variants between geQTL, eeQTL and sQTL within each tissue, and the number of common variants between liver and muscle within each eQTL type were all significantly (P < 0.05) larger than expected by chance. The identified eQTL were significantly (P < 0.05) enriched in GWAS hits associated with 56 carcass traits and fatty acid profiles. For example, several geQTL in muscle mapped to the FAM184B gene, hundreds of sQTL in liver and muscle mapped to the CAST gene, and hundreds of sQTL in liver mapped to the C6 gene. These three genes are associated with body composition or fatty acid profiles.ConclusionsWe detected a large number of significant eQTL and found that the overlap of variants between eQTL types and tissues was prevalent. Many eQTL were also QTL for meat traits. Our study fills a gap in the knowledge on the regulatory variants and their role in complex traits for the sheep model.

Polymorphism of CAST gene in five sheep breeds in Bulgaria

CAST gene is a candidate marker that influences the intensity of growth and meat quality. The aim of this study was to investigate the genetic variability of CAST gene in five Bulgarian sheep breeds – two merino, two local and one for milk. A total of 150 ewes, belonging to these breeds were investigated for polymorphisms of CAST gene by using PCR-RFLP method. A 622 bp fragment of Ovis aries CAST gene was amplified using PCR. After restriction with endonuclease MspI two alleles were observed in Ascanian, Caucasian, Breznik and Pleven Blackhead breeds. One allele (M) and one genotype (MM) were detected in Cooper-Red Shumen breed. The highest frequency of the allele N was established in the Ascanian merino breed (0.27) followed by Caucasian merino (0.13), Breznik (0.12) and Pleven Blackhead (0.07). Two genotypes - MM and MN, were observed in Caucasian, Breznik and Pleven Blackhead breeds. The three possible genotypes were found only in Ascanian merino ewes - MM, MN and NN. The lowest frequency of the homozygous genotype MM (0.50) and the highest frequency of the heterozygous genotype MN was established in the Ascanian merino breed (0.47). The obtained results confirm that the PCR-RFLP method can be used to identify different genotypic variation of CAST gene in Bulgarian sheep breeds. The established genetic diversity in the calpastatin gene indicates that, after further associative studies, this gene may be included in the breeding programs of certain sheep breeds.

ЖИРНОКИСЛОТНЫЙ СОСТАВ ЛИПИДОВ МЫШЕЧНОЙ ТКАНИ МОЛОДНЯКА ОВЕЦ РАЗНЫХ АЛЛЕЛЬНЫХ ВАРИАНТОВ ГЕНА CAST

The article presents the results of the analysis of the fatty acid composition of the lipids of the muscle tissue of sheep of diff erent genotypes of the Stavropol breed at the age of 8 months. The polymorphism of the calpastatin CAST gene was determined using PCR-PDRF.

Polymorphism of gh, cast genes in sheep in connection with resistance indicators

The article is devoted to one of the important problems of improving the safety and vitality of young animals. Progress in biotechnology and molecular genetics allows one to give an objective assessment of the genetic potential, to predict the resistance of the animal organism to diseases, unfavorable environmental factors at the early stages of postnatal ontogenesis. One of the advantages of DNA diagnostics is the ability to assess animals at birth. DNA diagnostics allows to obtain a large amount of information in a short time, as well as to determine the polymorphism of genes that control the characteristics of productivity in sheep. This allows you to objectively assess the prospects of young animals and select animals for inclusion in the selection process, in the early period of ontogenesis. In this regard, the goal was set to study the polymorphism of the somatotropin - GH, calpastatin - CAST genes and its relationship with the parameters of the immune reactivity of lambs of different genotypes. Using the PCR-RFLP method, polymorphism of genes, GH, CAST, represented by two GHA alleles, was revealed; GHB and CASTM; СASTN with different frequency of occurrence and three genotypes: GHAA, GHBB, GHAB; СASTММ, СASTNN, СASTМN. Taking into account the results of genotyping, experimental groups were formed: I - carriers of GHAA; II - GHBB; III - GHAB; IV - СASTММ; V– СASTNN; VI - СASTМN genotype. The genotypes of the carriers of marker alleles were revealed. Immune reactivity was assessed by the level of immunocompetent cells (T-, B-lymphocytes) and the ratio of their subpopulations (T-helpers; T-suppressors). The relationship between the size of live weight, average daily gains, and immune reactivity of different genotypes was established. The superiority in terms of live weight of lambs with genotypes GHBB and GHAB compared to GHAA CASTMN; which averaged 0.9 and 5.8%. The revealed regularity can serve as an evaluative criterion of the viability and productivity of sheep at an early age.

Associations of CAST, CAPN1 and MSTN Genes Polymorphism with Slaughter Value and Beef Quality – A Review

Abstract The slaughter value of cattle and beef quality are influenced by many factors, which can generally be divided into antemortem (breed, sex, age, housing system, diet, pre-slaughter handling) and postmortem (post-slaughter processing, chilling temperature, packaging). Studies of many authors have shown that meat quality traits can be also influenced by the individual genetic background of an animal. Numerous studies have been conducted worldwide to determine the functions of various genes as well as polymorphisms with potential effects on fattening and slaughter value of cattle and on beef quality. This study reviews the most important research done on the associations of polymorphisms in the calpain, calpastatin and myostatin genes with carcass traits and beef quality. Knowledge about the genes and chromosome regions associated with desired meat quality characteristics may prove very helpful when selecting pairs for mating and estimating the breeding value of offspring, mainly because it is difficult to improve meat quality traits based on conventional selection methods due to their low heritability and polygenic regulation. Furthermore, meat quality evaluation is expensive and can only be carried out after slaughter.

Association between polymorphisms of Myf5, MSTN and CAST genes and fattening performance in Brown Swiss and Holstein cattle breeds

This study determined the association between Myf5, CAST and MSTN genes and fattening performance traits in 228 (Holstein = 103 and Brown = 125) cattle breeds. Classification and regression tree (CART) was used to determine association between genes and fattening performance. The allele frequencies of Holstein cattle in terms of Myf5, CAST and MSTN genes were A:0.30, B:0.70; A:0.43, G:0.56; A:0.97, B:0.03, respectively, whereas in the Brown Swiss cattle were A:0.36, B:0.64; A:0.65, G:0.34; A:0.88, B:0.12 respectively. The Myf5, CAST, and MSTN genes were found to be in the Hardy–Weinberg equilibrium (p > 0.05) in both of the cattle breeds. As a result, the association between Myf5, CAST and MSTN genes and fattening performance traits were found to be significant according to CART analysis.

Investigating new SNPs of CAST, CAPN and SCD genes in 5’UTR of Bali cattle

Abstract. Jakaria, Ulum MF, Lestari D, Akwila S, Sihite DEWT, Priyanto R, Muladno, Sumantri C. 2020. Investigating new SNPs of CAST, CAPN and SCD genes in 5’UTR of Bali cattle. Biodiversitas 21: 2971-2976. The present investigation aims to explore specific SNPs of Bali cattle, focusing mainly on CAST, CAPN, and SCD genes in 5'UTR. DNA of Bali cattle (25 individuals) from Breeding Centre of Bali cattle in Bali Province, Indonesia, were extracted according to the protocol of GenAID. Amplification of the CAST, CAPN, and SCD genes were designed using the Primer3 program based on reference sequences (access numbers AH014526.2, AH009246.3, and AY241932). Furthermore, the PCR product was sequenced through forward primer sequencing. The data sequence results were analyzed using the Bioedit, MEGA7, Popgen 3.2, and DnaSP6 V6 programs. The results showed that SNPs were detected at g.921C&gt;T and g.922T&gt;G in 5'UTR for the CAST gene between the reference of GenBank AH014526.2 and Bali cattle, but it was monomorphic within the population. The high polymorphism of SNPs was found in g.232G&gt;T for the CAPN gene and g.134A&gt;del for the SCD gene. However, the SNP g.246C&gt;del SCD gene was detected as monomorphic in this region. Moreover, this result shows that low nucleotide diversities (Pi) for CAST, CAPN, and SCD genes in 5'UTR of Bali cattle were 0.00422, 0.00632, and 0.00449, respectively. This finding was important for developing marker-assisted selection (MAS) to improve the meat quality of Bali cattle.

Features of polymorphism of calpastatin and somatotropin genes in young sheep, obtained from crossing ewes of Kalmyk fat-rumped sheeps and dorper rams

The article presents the study of polymorphism of the CAST and GH genes, which determine the features of the manifestation of the productive and biological characteristics of sheep with ½ Kalmyk + ½ Dorper blood system. Calpastatin gene polymorphism represented by alleles M and N, whose frequency was 0,65 and 0,35; genotypes MM, MN – 30 and 70% accordingly. The desired NN genotype has not been identified. A relatively uniform incidence of allele N (0,35) CAST gene and B (0,40) GH was established, what contributed to an almost equal distribution of allele frequencies М (0,65) and А (0,60) genes of calpastatin and somatotropin accordingly. The frequency of heterozygous genotypes occurrence by CAST gene composed 0,7. By GH gene the following distribution of genotype frequencies is observed. Frequency of occurrence homozygous АА and heterozygous AB genotypes was equal to and is 0,4, wherein the frequency of occurrence advised homozygous ВВ genotype was 0,2. Among the animals studied, sheep with a complex genotype are most common CASTMNGHAB (40 %). The amount of percent CASTMMGHAA and CASTMNGHAA is in 22,2%. 10% is for genotypes CASTMMGHBB and CASTMNGHBB.

Research Article Polymorphism of CAST and GDF-8 genes and association with physical-chemical parameters of sheep meat

Research Article Polymorphism of CAST and GDF-8 genes and association with physical-chemical parameters of sheep meat

Association of CAST-gene polymorphism with mRNA levels and meat tenderness in goats

Context Meat tenderness is affected by numerous factors that can cause considerable economic loss as it is one of the characteristics of meat most appreciated by consumers. Higher expression of the calpastatin gene (CAST) has been associated with a reduced meat tenderness in different animal species. Aims Our main objective of the present study was to evaluate the association of the expression of the CAST gene with the shear force of goat muscle. We also assessed whether variations in gene expression could be explained by the polymorphism already identified by other authors or whether the polymorphism may be associated with phenotypic characteristics such as meat tenderness, slaughter weight and carcass weight. Methods Forty crossbred goats (Saanen × Alpine) were slaughtered at ~150 days of age. So as to obtain the genotype of each animal, blood samples were collected on the day of slaughter for subsequent DNA isolation. On the same day, samples of muscle tissue (Longissimus lumborum) were collected to analyse both gene expression and shear force. Key results Among the seven alleles already known from A to G, the C allele, which is the only one exhibiting exon variation, was not observed in our samples. We found, for the first time, that in goat, the highest CAST-gene expression levels are directly related to an increase in the muscle shear force, as has been described for other species. No statistical difference was observed for shear force, slaughter weight or carcass weight among the different genotypic groups evaluated. However, we observed that the level of expression of the CAST gene within Group 7 (Genotypes GG and GE) presented a higher value that in the other genotypes (P &amp;lt; 0.05), although we did not find a satisfactory explanation for the increase in expression in those genotypes. Conclusions Our results gave support what has already been verified in studies with other species, namely that the calpastatin-gene expression is related to the postmortem meat-tenderising process. Implications The tenderness is an important feature in the choice of meat. Several factors can alter this characteristic, such as breed, age, sex and slaughter weight of animals. The molecular mechanisms involved in ante-morten and postmortem processes are important for improving understanding of how we can identify animals that tend to have softer meat after slaughter by applying new technologies, such as molecular marker.

Next Generation Sequencing (NGS) Based Variation Analysis: A New Practical Biomarker for Beef Tenderness Assessment

Evaluation of some meat quality attributes using genetic analysis is steadily increasing. PCR based targeted variation analysis is one of the most commonly preferred techniques for this purpose. Recently, Next Generation Sequencing (NGS) method has drawn considerable attention because of its’ high analysis capacity. The purpose of the current study was to determine variations in CAST gene from Brangus and Simmental cattle by performing whole gene sequencing using NGS, and to investigate the potential of NGS method in evaluating meat tenderness based on the high genomic data it provides. Whole gene sequence analysis was performed on Calpastatin (CAST) gene of samples acquired from 52 Brangus and 52 Simmental beef cattle breeds using NGS method, and the variations detected were evaluated in terms of their potential in measuring meat tenderness and quality. NGS outputs were analyzed in Ensemble “cow” database platform and 13 variations were detected. One of these variations (EXON 8 c.439C&amp;gt;G/ p.L147V ) was evaluated as undeclared before. In 20 Brangus cattle and in 9 Simmental cattle, no variations were detected whereas 6 variations (V1, V2, V5, V8, V10 and V13) were found significantly different (p&amp;lt;0.05) based on their distribution in breeds.

Study of the frequencies of the single-nucleotide polymorphism Ser638Arg of the CAST gene in pigs of the crossbred PIC

Study of the frequencies of the single-nucleotide polymorphism Ser638Arg of the CAST gene in pigs of the crossbred PIC

The use of single-nucleotide polymorphism in creating a crossline of meat simmentals

The article presents the materials of the study on bull cross of the Bredy meat type simmentals, which include the genotyping of animals for the CAST and CAPN1 genes with the identification of frequencies of genotype and alleles, as well as testing the production of these animals expressed in the evaluation of aged within 8 days of meat for the indicators tenderness, juiciness, organoleptic assessment of taste. The results of the studies did not confirm, with certainty, the influence of the CAST gene polymorphism on the beef tenderness indicators, as well as no associations of this gene with the studied juiciness and meat taste indicators, while the carriers of the homozygous CC genotype CAPN1 exceeded other genotypes of the sample in tenderness, juiciness and taste of beef. And indicators of bull meat with a combination of TT* genes on CAST and CC* on CAPN1 had superiority over the average indicators of the total sample (P <0.001), as well as over the group of animals with the most preferred CC genotype for the CAPN1 gene by 1.22 points or 15.4% (P <0.01) by tenderness, by 1.44 points or 18.2% (P <0.05) by juiciness, by 1.56 points or 19.2% (P <0.001) by taste. Studies have confirmed the effect of the CAPN1 gene CC homozygotes polymorphic state itself, as well as the CAST and CAPN1 genes on beef tenderness in the part where the combination of TT genotypes in the CAST gene and CC in the CAPN1 gene gives a positive effect on beef tenderness, probably associated with activation the activity of μ-calpain in connection with the weakening of the effect of calpastatin as an inhibitor on μ-calpain, as a result of which the enzymatic effect on myofibrillary proteins acquires a different intensity, and possibly a different manifestation. What was the reason for the increase in sensory sensitivity to taste of prepared samples, tenderness and juiciness of meat.